Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS
Abstract
SufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe–S cluster assembly pathway. In Escherichia coli, this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically, this means SufS must shift between protecting a covalent persulfide intermediate and making it available for transfer to the next protein partner in the pathway, SufE. Here, we report five X-ray crystal structures of SufS including a new structure of SufS containing an inward-facing persulfide intermediate on C364. Additional structures of SufS variants with substitutions at the dimer interface show changes in dimer geometry and suggest a conserved β-hairpin structure plays a role in mediating interactions with SufE. Here, these new structures, along with previous HDX-MS and biochemical data, identify an interaction network capable of communication between active-sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities.
- Authors:
-
- Univ. of Alabama, Tuscaloosa, AL (United States)
- Univ. of South Carolina, Columbia, SC (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health
- OSTI Identifier:
- 1494761
- Grant/Contract Number:
- W-31-109-Eng-38; S10_RR25528; S10_RR028976; GM112919
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biochemistry
- Additional Journal Information:
- Journal Volume: 58; Journal Issue: 6; Journal ID: ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; interfaces; peptides and proteins; monomers; crystal structure; oligomers
Citation Formats
Dunkle, Jack A., Bruno, Michael R., Outten, F. Wayne, and Frantom, Patrick A. Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS. United States: N. p., 2018.
Web. doi:10.1021/acs.biochem.8b01122.
Dunkle, Jack A., Bruno, Michael R., Outten, F. Wayne, & Frantom, Patrick A. Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS. United States. https://doi.org/10.1021/acs.biochem.8b01122
Dunkle, Jack A., Bruno, Michael R., Outten, F. Wayne, and Frantom, Patrick A. Thu .
"Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS". United States. https://doi.org/10.1021/acs.biochem.8b01122. https://www.osti.gov/servlets/purl/1494761.
@article{osti_1494761,
title = {Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS},
author = {Dunkle, Jack A. and Bruno, Michael R. and Outten, F. Wayne and Frantom, Patrick A.},
abstractNote = {SufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe–S cluster assembly pathway. In Escherichia coli, this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically, this means SufS must shift between protecting a covalent persulfide intermediate and making it available for transfer to the next protein partner in the pathway, SufE. Here, we report five X-ray crystal structures of SufS including a new structure of SufS containing an inward-facing persulfide intermediate on C364. Additional structures of SufS variants with substitutions at the dimer interface show changes in dimer geometry and suggest a conserved β-hairpin structure plays a role in mediating interactions with SufE. Here, these new structures, along with previous HDX-MS and biochemical data, identify an interaction network capable of communication between active-sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities.},
doi = {10.1021/acs.biochem.8b01122},
journal = {Biochemistry},
number = 6,
volume = 58,
place = {United States},
year = {Thu Dec 20 00:00:00 EST 2018},
month = {Thu Dec 20 00:00:00 EST 2018}
}
Web of Science
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Works referencing / citing this record:
Hydrogen Sulfide From Cysteine Desulfurase, Not 3-Mercaptopyruvate Sulfurtransferase, Contributes to Sustaining Cell Growth and Bioenergetics in E. coli Under Anaerobic Conditions
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