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Title: Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope

The identification of isotopic labels by conventional macroscopic techniques lacks spatial resolution and requires relatively large quantities of material for measurements. We recorded the vibrational spectra of an α amino acid,l-alanine, with damage-free “aloof” electron energy-loss spectroscopy in a scanning transmission electron microscope to directly resolve carbon-site–specific isotopic labels in real space with nanoscale spatial resolution. An isotopic red shift of 4.8 ± 0.4 milli–electron volts in C–O asymmetric stretching modes was observed for 13C-labeledl-alanine at the carboxylate carbon site, which was confirmed by macroscopic infrared spectroscopy and theoretical calculations. The accurate measurement of this shift opens the door to nondestructive, site-specific, spatially resolved identification of isotopically labeled molecules with the electron microscope.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ;  [2] ; ORCiD logo [2] ; ORCiD logo [2] ; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Nion R&D, Kirkland, WA (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Science
Additional Journal Information:
Journal Volume: 363; Journal Issue: 6426; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
74 ATOMIC AND MOLECULAR PHYSICS
OSTI Identifier:
1493991

Hachtel, Jordan A., Huang, Jingsong, Popovs, Ilja, Jansone-Popova, Santa, Keum, Jong K., Jakowski, Jacek, Lovejoy, Tracy C., Dellby, Niklas, Krivanek, Ondrej L., and Idrobo, Juan Carlos. Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope. United States: N. p., Web. doi:10.1126/science.aav5845.
Hachtel, Jordan A., Huang, Jingsong, Popovs, Ilja, Jansone-Popova, Santa, Keum, Jong K., Jakowski, Jacek, Lovejoy, Tracy C., Dellby, Niklas, Krivanek, Ondrej L., & Idrobo, Juan Carlos. Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope. United States. doi:10.1126/science.aav5845.
Hachtel, Jordan A., Huang, Jingsong, Popovs, Ilja, Jansone-Popova, Santa, Keum, Jong K., Jakowski, Jacek, Lovejoy, Tracy C., Dellby, Niklas, Krivanek, Ondrej L., and Idrobo, Juan Carlos. 2019. "Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope". United States. doi:10.1126/science.aav5845.
@article{osti_1493991,
title = {Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope},
author = {Hachtel, Jordan A. and Huang, Jingsong and Popovs, Ilja and Jansone-Popova, Santa and Keum, Jong K. and Jakowski, Jacek and Lovejoy, Tracy C. and Dellby, Niklas and Krivanek, Ondrej L. and Idrobo, Juan Carlos},
abstractNote = {The identification of isotopic labels by conventional macroscopic techniques lacks spatial resolution and requires relatively large quantities of material for measurements. We recorded the vibrational spectra of an α amino acid,l-alanine, with damage-free “aloof” electron energy-loss spectroscopy in a scanning transmission electron microscope to directly resolve carbon-site–specific isotopic labels in real space with nanoscale spatial resolution. An isotopic red shift of 4.8 ± 0.4 milli–electron volts in C–O asymmetric stretching modes was observed for13C-labeledl-alanine at the carboxylate carbon site, which was confirmed by macroscopic infrared spectroscopy and theoretical calculations. The accurate measurement of this shift opens the door to nondestructive, site-specific, spatially resolved identification of isotopically labeled molecules with the electron microscope.},
doi = {10.1126/science.aav5845},
journal = {Science},
number = 6426,
volume = 363,
place = {United States},
year = {2019},
month = {2}
}

Works referenced in this record:

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