Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope

Hachtel, Jordan A.; Huang, Jingsong; Popovs, Ilja; Jansone-Popova, Santa; Keum, Jong K.; Jakowski, Jacek; Lovejoy, Tracy C.; Dellby, Niklas; Krivanek, Ondrej L.; Idrobo, Juan Carlos

doi:10.1126/science.aav5845

Title: Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope

Abstract

The identification of isotopic labels by conventional macroscopic techniques lacks spatial resolution and requires relatively large quantities of material for measurements. We recorded the vibrational spectra of an α amino acid, l -alanine, with damage-free “aloof” electron energy-loss spectroscopy in a scanning transmission electron microscope to directly resolve carbon-site–specific isotopic labels in real space with nanoscale spatial resolution. An isotopic red shift of 4.8 ± 0.4 milli–electron volts in C–O asymmetric stretching modes was observed for ¹³ C-labeled l -alanine at the carboxylate carbon site, which was confirmed by macroscopic infrared spectroscopy and theoretical calculations. The accurate measurement of this shift opens the door to nondestructive, site-specific, spatially resolved identification of isotopically labeled molecules with the electron microscope.

Authors:

;

; Lovejoy, Tracy C.;

;

Publication Date:: Thu Jan 31 00:00:00 EST 2019

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1528642

Alternate Identifier(s):: OSTI ID: 1493991

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Published Article

Journal Name:: Science

Additional Journal Information:: Journal Name: Science Journal Volume: 363 Journal Issue: 6426; Journal ID: ISSN 0036-8075

Publisher:: American Association for the Advancement of Science (AAAS)

Country of Publication:: United States

Language:: English

Subject:: 74 ATOMIC AND MOLECULAR PHYSICS

Citation Formats


                    Hachtel, Jordan A., Huang, Jingsong, Popovs, Ilja, Jansone-Popova, Santa, Keum, Jong K., Jakowski, Jacek, Lovejoy, Tracy C., Dellby, Niklas, Krivanek, Ondrej L., and Idrobo, Juan Carlos. Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope.  United States: N. p., 2019. 
Web.  doi:10.1126/science.aav5845.

Copy to clipboard


                    Hachtel, Jordan A., Huang, Jingsong, Popovs, Ilja, Jansone-Popova, Santa, Keum, Jong K., Jakowski, Jacek, Lovejoy, Tracy C., Dellby, Niklas, Krivanek, Ondrej L., & Idrobo, Juan Carlos. Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope.  United States.  https://doi.org/10.1126/science.aav5845

Copy to clipboard


                    Hachtel, Jordan A., Huang, Jingsong, Popovs, Ilja, Jansone-Popova, Santa, Keum, Jong K., Jakowski, Jacek, Lovejoy, Tracy C., Dellby, Niklas, Krivanek, Ondrej L., and Idrobo, Juan Carlos. Thu .  
"Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope".  United States.  https://doi.org/10.1126/science.aav5845.

Copy to clipboard


                    
@article{osti_1528642,

  title        = {Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope},

  author       = {Hachtel, Jordan A. and Huang, Jingsong and Popovs, Ilja and Jansone-Popova, Santa and Keum, Jong K. and Jakowski, Jacek and Lovejoy, Tracy C. and Dellby, Niklas and Krivanek, Ondrej L. and Idrobo, Juan Carlos},

  abstractNote = {The identification of isotopic labels by conventional macroscopic techniques lacks spatial resolution and requires relatively large quantities of material for measurements. We recorded the vibrational spectra of an α amino acid, l -alanine, with damage-free “aloof” electron energy-loss spectroscopy in a scanning transmission electron microscope to directly resolve carbon-site–specific isotopic labels in real space with nanoscale spatial resolution. An isotopic red shift of 4.8 ± 0.4 milli–electron volts in C–O asymmetric stretching modes was observed for 13 C-labeled l -alanine at the carboxylate carbon site, which was confirmed by macroscopic infrared spectroscopy and theoretical calculations. The accurate measurement of this shift opens the door to nondestructive, site-specific, spatially resolved identification of isotopically labeled molecules with the electron microscope.},

  doi          = {10.1126/science.aav5845},

  journal      = {Science},

  number       = 6426,

  volume       = 363,

  place        = {United States},

  year         = {Thu Jan 31 00:00:00 EST 2019},

  month        = {Thu Jan 31 00:00:00 EST 2019}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Publisher's Version of Record
https://doi.org/10.1126/science.aav5845

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 75 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Exploring the capabilities of monochromated electron energy loss spectroscopy in the infrared regime
journal, April 2018

Hachtel, Jordan A.; Lupini, Andrew R.; Idrobo, Juan Carlos
Scientific Reports, Vol. 8, Issue 1
DOI: 10.1038/s41598-018-23805-5

Nanoscale momentum-resolved vibrational spectroscopy
journal, June 2018

Hage, Fredrik S.; Nicholls, Rebecca J.; Yates, Jonathan R.
Science Advances, Vol. 4, Issue 6
DOI: 10.1126/sciadv.aar7495

Vibrational spectroscopy with neutrons—Where are we now?
journal, May 2010

Kearley, G. J.; Johnson, M. R.
Vibrational Spectroscopy, Vol. 53, Issue 1
DOI: 10.1016/j.vibspec.2009.11.006

First-Principles Vibrational Electron Energy Loss Spectroscopy of $β$ -Guanine
journal, July 2017

Radtke, G.; Taverna, D.; Lazzeri, M.
Physical Review Letters, Vol. 119, Issue 2
DOI: 10.1103/PhysRevLett.119.027402

Isotope analysis in the transmission electron microscope
journal, October 2016

Susi, Toma; Hofer, Christoph; Argentero, Giacomo
Nature Communications, Vol. 7, Issue 1
DOI: 10.1038/ncomms13040

Vibrational spectroscopy in the electron microscope
journal, October 2014

Krivanek, Ondrej L.; Lovejoy, Tracy C.; Dellby, Niklas
Nature, Vol. 514, Issue 7521
DOI: 10.1038/nature13870

Probing low-energy hyperbolic polaritons in van der Waals crystals with an electron microscope
journal, July 2017

Govyadinov, Alexander A.; Konečná, Andrea; Chuvilin, Andrey
Nature Communications, Vol. 8, Issue 1
DOI: 10.1038/s41467-017-00056-y

Scattering delocalization and radiation damage in STEM-EELS
journal, September 2017

Egerton, R. F.
Ultramicroscopy, Vol. 180
DOI: 10.1016/j.ultramic.2017.02.007

Nanoscale Probing of Local Hydrogen Heterogeneity in Disordered Carbon Nitrides with Vibrational Electron Energy-Loss Spectroscopy
journal, May 2018

Haiber, Diane M.; Crozier, Peter A.
ACS Nano, Vol. 12, Issue 6
DOI: 10.1021/acsnano.8b00884

Hyperpolarized 13C Lactate, Pyruvate, and Alanine: Noninvasive Biomarkers for Prostate Cancer Detection and Grading
journal, October 2008

Albers, M. J.; Bok, R.; Chen, A. P.
Cancer Research, Vol. 68, Issue 20
DOI: 10.1158/0008-5472.CAN-08-0749

Structural analysis and mapping of individual protein complexes by infrared nanospectroscopy
journal, December 2013

Amenabar, Iban; Poly, Simon; Nuansing, Wiwat
Nature Communications, Vol. 4, Issue 1
DOI: 10.1038/ncomms3890

Monochromated STEM with a 30 meV-wide, atom-sized electron probe
journal, January 2013

Krivanek, Ondrej L.; Lovejoy, Tracy C.; Dellby, Niklas
Microscopy, Vol. 62, Issue 1
DOI: 10.1093/jmicro/dfs089

Local low rank denoising for enhanced atomic resolution imaging
journal, April 2018

Spiegelberg, Jakob; Idrobo, Juan Carlos; Herklotz, Andreas
Ultramicroscopy, Vol. 187
DOI: 10.1016/j.ultramic.2018.01.012

Vibrational Spectroscopy of Water with High Spatial Resolution
journal, July 2018

Jokisaari, Jacob R.; Hachtel, Jordan A.; Hu, Xuan
Advanced Materials, Vol. 30, Issue 36
DOI: 10.1002/adma.201802702

Precision neutron diffraction structure determination of protein and nucleic acid components. I. Crystal and molecular structure of the amino acid L-alanine
journal, April 1972

Lehmann, M. S.; Koetzle, T. F.; Hamilton, W. C.
Journal of the American Chemical Society, Vol. 94, Issue 8
DOI: 10.1021/ja00763a016

Vibrational and valence aloof beam EELS: A potential tool for nondestructive characterization of nanoparticle surfaces
journal, September 2017

Crozier, Peter A.
Ultramicroscopy, Vol. 180
DOI: 10.1016/j.ultramic.2017.03.011

Identification of individual isotopes in a polymer blend using tip enhanced Raman spectroscopy: Identification of individual isotopes in a polymer blend
journal, March 2015

Agapov, Rebecca L.; Scherger, Jacob D.; Sokolov, Alexei P.
Journal of Raman Spectroscopy, Vol. 46, Issue 5
DOI: 10.1002/jrs.4671

Uncertainties in Scaling Factors for ab Initio Vibrational Frequencies
journal, September 2005

Irikura, Karl K.; Johnson, Russell D.; Kacker, Raghu N.
The Journal of Physical Chemistry A, Vol. 109, Issue 37
DOI: 10.1021/jp052793n

The effect of pressure on the crystal structure of l-alanine
journal, January 2010

Funnell, Nicholas P.; Dawson, Alice; Francis, Duncan
CrystEngComm, Vol. 12, Issue 9
DOI: 10.1039/c001296c

Towards weighing individual atoms by high-angle scattering of electrons
journal, April 2015

Argentero, G.; Mangler, C.; Kotakoski, J.
Ultramicroscopy, Vol. 151
DOI: 10.1016/j.ultramic.2014.11.031

Transparent Window Vibrational Probes for the Characterization of Proteins With High Structural and Temporal Resolution
journal, January 2017

Adhikary, Ramkrishna; Zimmermann, Jörg; Romesberg, Floyd E.
Chemical Reviews, Vol. 117, Issue 3
DOI: 10.1021/acs.chemrev.6b00625

The infrared absorption of amino acid side chains
journal, January 2000

Barth, Andreas
Progress in Biophysics and Molecular Biology, Vol. 74, Issue 3-5
DOI: 10.1016/S0079-6107(00)00021-3

Advances in molecular quantum chemistry contained in the Q-Chem 4 program package
journal, September 2014

Shao, Yihan; Gan, Zhengting; Epifanovsky, Evgeny
Molecular Physics, Vol. 113, Issue 2
DOI: 10.1080/00268976.2014.952696

Structure and Vibrational Spectra of the Zwitterion l -Alanine in the Presence of Explicit Water Molecules: A Density Functional Analysis
journal, July 1998

Tajkhorshid, Emadeddin; Jalkanen, K. J.; Suhai, Sándor
The Journal of Physical Chemistry B, Vol. 102, Issue 30
DOI: 10.1021/jp9803135

The 3.8 A resolution cryo-EM structure of Zika virus
journal, March 2016

Sirohi, D.; Chen, Z.; Sun, L.
Science, Vol. 352, Issue 6284
DOI: 10.1126/science.aaf5316

Two-dimensional IR spectroscopy and isotope labeling defines the pathway of amyloid formation with residue-specific resolution
journal, April 2009

Shim, S. -H.; Gupta, R.; Ling, Y. L.
Proceedings of the National Academy of Sciences, Vol. 106, Issue 16
DOI: 10.1073/pnas.0805957106

Mapping vibrational surface and bulk modes in a single nanocube
journal, March 2017

Lagos, Maureen J.; Trügler, Andreas; Hohenester, Ulrich
Nature, Vol. 543, Issue 7646
DOI: 10.1038/nature21699

Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms
journal, June 2003

Radajewski, Stefan; McDonald, Ian R.; Murrell, J. Colin
Current Opinion in Biotechnology, Vol. 14, Issue 3, p. 296-302
DOI: 10.1016/S0958-1669(03)00064-8

Damage-free vibrational spectroscopy of biological materials in the electron microscope
journal, March 2016

Rez, Peter; Aoki, Toshihiro; March, Katia
Nature Communications, Vol. 7, Issue 1
DOI: 10.1038/ncomms10945

Electron-Beam Mapping of Vibrational Modes with Nanometer Spatial Resolution
journal, December 2016

Dwyer, C.; Aoki, T.; Rez, P.
Physical Review Letters, Vol. 117, Issue 25
DOI: 10.1103/PhysRevLett.117.256101

Development of electron energy-loss spectroscopy in the biological sciences
journal, January 2012

Aronova, M. A.; Leapman, R. D.
MRS Bulletin, Vol. 37, Issue 1
DOI: 10.1557/mrs.2011.329

Temperature Measurement by a Nanoscale Electron Probe Using Energy Gain and Loss Spectroscopy
journal, March 2018

Idrobo, Juan Carlos; Lupini, Andrew R.; Feng, Tianli
Physical Review Letters, Vol. 120, Issue 9
DOI: 10.1103/PhysRevLett.120.095901

Works referencing / citing this record:

Emerging Electron Microscopy Techniques for Probing Functional Interfaces in Energy Materials
journal, October 2019

Zachman, Michael J.; Hachtel, Jordan A.; Idrobo, Juan Carlos
Angewandte Chemie, Vol. 132, Issue 4
DOI: 10.1002/ange.201902993

Spectrally tunable infrared plasmonic F,Sn:In ₂ O ₃ nanocrystal cubes
journal, January 2020

Cho, Shin Hum; Roccapriore, Kevin M.; Dass, Chandriker Kavir
The Journal of Chemical Physics, Vol. 152, Issue 1
DOI: 10.1063/1.5139050

Far-field midinfrared superresolution imaging and spectroscopy of single high aspect ratio gold nanowires
journal, January 2020

Aleshire, Kyle; Pavlovetc, Ilia M.; Collette, Robyn
Proceedings of the National Academy of Sciences, Vol. 117, Issue 5
DOI: 10.1073/pnas.1916433117

Polarization Selectivity in Vibrational Electron-Energy-Loss Spectroscopy
journal, December 2019

Radtke, G.; Taverna, D.; Menguy, N.
Physical Review Letters, Vol. 123, Issue 25
DOI: 10.1103/physrevlett.123.256001

Efficient and Versatile Model for Vibrational STEM-EELS
journal, January 2020

Zeiger, Paul M.; Rusz, Ján
Physical Review Letters, Vol. 124, Issue 2
DOI: 10.1103/physrevlett.124.025501

Isotopic compositions, nitrogen functional chemistry, and low‐loss electron spectroscopy of complex organic aggregates at the nanometer scale in the carbonaceous chondrite Renazzo
journal, October 2019

Vollmer, Christian; Leitner, Jan; Kepaptsoglou, Demie
Meteoritics & Planetary Science, Vol. 55, Issue 6
DOI: 10.1111/maps.13389

Similar Records in DOE PAGES and OSTI.GOV collections:

Damage-free vibrational spectroscopy of biological materials in the electron microscope

Journal Article Rez, Peter ; Aoki, Toshihiro ; March, Katia ; ... - Nature Communications

Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an ‘aloof’ electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies o1 eV can be ‘safely’ investigated. To demonstrate the potential of aloof spectroscopy, we record electron energy loss spectra from biogenic guanine crystals in their native state, resolving their characteristic C–H, N–H and C=O vibrational signatures with nomore »« less
Cited by 96
https://doi.org/10.1038/ncomms10945

Full Text Available
Direct Nanoscale Characterization of Deep Levels in AgCuInGaSe₂ Using Electron Energy–Loss Spectroscopy in the Scanning Transmission Electron Microscope

Journal Article Deitz, Julia I. ; Paul, Pran K. ; Farshchi, Rouin ; ... - Advanced Energy Materials

A new experimental framework for the characterization of defects in semiconductors is demonstrated. Through the direct, energy-resolved correlation of three analytical techniques spanning six orders of magnitude in spatial resolution, a critical mid-bandgap electronic trap level (E_V + 0.56 eV) within Ag_0.2Cu_0.8In_1–xGa_xSe₂ is traced to its nanoscale physical location and chemical source. This is achieved through a stepwise, site-specific correlated characterization workflow consisting of device-scale (≈1 mm²) deep level transient spectroscopy (DLTS) to survey the traps present, scanning probe–based DLTS (scanning-DLTS) for mesoscale-resolved (hundreds of nanometers) mapping of the target trap state’s spatial distribution, and scanning transmission electron microscope basedmore »« less
Cited by 12
https://doi.org/10.1002/aenm.201901612

Full Text Available
D-Band EPR and ENDOR Spectroscopy of ¹⁵N-Labeled Photosystem I

Journal Article Niklas, Jens ; Brahmachari, Udita ; Utschig, Lisa M. ; ... - Applied Magnetic Resonance

For billions of years, nature has optimized the photosynthetic machinery that converts light energy into chemical energy. Key primary reactions of photosynthesis occur in large membrane protein-cofactor complexes. The light-induced sequential electron transfer reactions occur through a chain of donor/acceptor cofactors embedded in the protein matrix resulting in a long-lived transmembrane charge-separated state. EPR is the method of choice to study electron transfer and the interaction of protein environment with redox-active cofactors. However, the spectra of organic cofactor radicals typically are not fully resolved and severely overlap at conventional X-band EPR. Even at Q-band EPR, this overlap is present and often a serious problem. As a result, there is a large variation of the reported EPR data and limited understanding of electronic structures of several redox-active cofactors. These serious problems can often be overcome by the excellent spectral resolution provided by high-frequency EPR (HF EPR). In this work, we study the electronic structure of the primary electron donor P₇₀₀ and the secondary electron acceptor A₁ of Photosystem I (PSI) using 130 GHz (D-band) EPR and Electron-Nuclear-Double-Resonance (ENDOR) spectroscopy. PSI was isotopically labeled with ¹⁵N (I =more »« less
https://doi.org/10.1007/s00723-021-01438-8

Full Text Available
Development and implementation of an in situ high-resolution isotopic microscope for measuring metabolic interactions in soil mesocosms (Final Report)

Technical Report Shank, Elizabeth A. ; Anderton, Christopher R. ; Prabhakaran, Venkateshkumar ; ...

Many of our planet’s ecosystems rely on the activities of soil microbial communities. These microbes have significant and wide-ranging effects: they metabolize carbon and other nutrients, interact with plants and fungi, and perform other processes important to soil health. However, our ability to directly observe the enzymatic and metabolic activities of microbes within soil is currently limited, not only by the complexity of soil microbial communities themselves, but also by the lack of experimental tools to study them and their molecular interactions in situ. These challenges hinder our understanding of the life-sustaining processes of biomass decomposition and the manner inmore »« less
https://doi.org/10.2172/1987669

Full Text Available
Progress in ultrahigh energy resolution EELS

Journal Article Krivanek, Ondrej L. ; Dellby, Niklas ; Hachtel, Jordan A. ; ... - Ultramicroscopy

Electron energy loss spectroscopy (EELS) in the electron microscope has progressed remarkably in the last five years. Advances in monochromator and spectrometer design have improved the energy resolution attainable in a scanning transmission electron microscope (STEM) to 4.2 meV, and new applications of ultrahigh energy resolution EELS have not lagged behind. They include vibrational spectroscopy in the electron microscope, a field that did not exist 5 years ago but has now grown very substantially. Notable examples include vibrational mapping with about 1 nm spatial resolution, analyzing the momentum dependence of vibrational states in very small volumes, determining the local temperaturemore »« less
Cited by 66
https://doi.org/10.1016/j.ultramic.2018.12.006

Full Text Available

Similar Records

Title: Identification of site-specific isotopic labels by vibrational spectroscopy in the electron microscope

Abstract

Citation Formats

Exploring the capabilities of monochromated electron energy loss spectroscopy in the infrared regime journal, April 2018

Nanoscale momentum-resolved vibrational spectroscopy journal, June 2018

Vibrational spectroscopy with neutrons—Where are we now? journal, May 2010

First-Principles Vibrational Electron Energy Loss Spectroscopy of β -Guanine journal, July 2017

Isotope analysis in the transmission electron microscope journal, October 2016

Vibrational spectroscopy in the electron microscope journal, October 2014

Probing low-energy hyperbolic polaritons in van der Waals crystals with an electron microscope journal, July 2017

Scattering delocalization and radiation damage in STEM-EELS journal, September 2017

Nanoscale Probing of Local Hydrogen Heterogeneity in Disordered Carbon Nitrides with Vibrational Electron Energy-Loss Spectroscopy journal, May 2018

Hyperpolarized 13C Lactate, Pyruvate, and Alanine: Noninvasive Biomarkers for Prostate Cancer Detection and Grading journal, October 2008

Structural analysis and mapping of individual protein complexes by infrared nanospectroscopy journal, December 2013

Monochromated STEM with a 30 meV-wide, atom-sized electron probe journal, January 2013

Local low rank denoising for enhanced atomic resolution imaging journal, April 2018

Vibrational Spectroscopy of Water with High Spatial Resolution journal, July 2018

Precision neutron diffraction structure determination of protein and nucleic acid components. I. Crystal and molecular structure of the amino acid L-alanine journal, April 1972

Vibrational and valence aloof beam EELS: A potential tool for nondestructive characterization of nanoparticle surfaces journal, September 2017

Identification of individual isotopes in a polymer blend using tip enhanced Raman spectroscopy: Identification of individual isotopes in a polymer blend journal, March 2015

Uncertainties in Scaling Factors for ab Initio Vibrational Frequencies journal, September 2005

The effect of pressure on the crystal structure of l-alanine journal, January 2010

Towards weighing individual atoms by high-angle scattering of electrons journal, April 2015

Transparent Window Vibrational Probes for the Characterization of Proteins With High Structural and Temporal Resolution journal, January 2017

The infrared absorption of amino acid side chains journal, January 2000

Advances in molecular quantum chemistry contained in the Q-Chem 4 program package journal, September 2014

Structure and Vibrational Spectra of the Zwitterion l -Alanine in the Presence of Explicit Water Molecules: A Density Functional Analysis journal, July 1998

The 3.8 A resolution cryo-EM structure of Zika virus journal, March 2016

Two-dimensional IR spectroscopy and isotope labeling defines the pathway of amyloid formation with residue-specific resolution journal, April 2009

Mapping vibrational surface and bulk modes in a single nanocube journal, March 2017

Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms journal, June 2003

Damage-free vibrational spectroscopy of biological materials in the electron microscope journal, March 2016

Electron-Beam Mapping of Vibrational Modes with Nanometer Spatial Resolution journal, December 2016

Development of electron energy-loss spectroscopy in the biological sciences journal, January 2012

Temperature Measurement by a Nanoscale Electron Probe Using Energy Gain and Loss Spectroscopy journal, March 2018

Emerging Electron Microscopy Techniques for Probing Functional Interfaces in Energy Materials journal, October 2019

Spectrally tunable infrared plasmonic F,Sn:In 2 O 3 nanocrystal cubes journal, January 2020

Far-field midinfrared superresolution imaging and spectroscopy of single high aspect ratio gold nanowires journal, January 2020

Polarization Selectivity in Vibrational Electron-Energy-Loss Spectroscopy journal, December 2019

Efficient and Versatile Model for Vibrational STEM-EELS journal, January 2020

Isotopic compositions, nitrogen functional chemistry, and low‐loss electron spectroscopy of complex organic aggregates at the nanometer scale in the carbonaceous chondrite Renazzo journal, October 2019

Exploring the capabilities of monochromated electron energy loss spectroscopy in the infrared regime
journal, April 2018

Nanoscale momentum-resolved vibrational spectroscopy
journal, June 2018

Vibrational spectroscopy with neutrons—Where are we now?
journal, May 2010

First-Principles Vibrational Electron Energy Loss Spectroscopy of $β$ -Guanine
journal, July 2017

Isotope analysis in the transmission electron microscope
journal, October 2016

Vibrational spectroscopy in the electron microscope
journal, October 2014

Probing low-energy hyperbolic polaritons in van der Waals crystals with an electron microscope
journal, July 2017

Scattering delocalization and radiation damage in STEM-EELS
journal, September 2017

Nanoscale Probing of Local Hydrogen Heterogeneity in Disordered Carbon Nitrides with Vibrational Electron Energy-Loss Spectroscopy
journal, May 2018

Hyperpolarized 13C Lactate, Pyruvate, and Alanine: Noninvasive Biomarkers for Prostate Cancer Detection and Grading
journal, October 2008

Structural analysis and mapping of individual protein complexes by infrared nanospectroscopy
journal, December 2013

Monochromated STEM with a 30 meV-wide, atom-sized electron probe
journal, January 2013

Local low rank denoising for enhanced atomic resolution imaging
journal, April 2018

Vibrational Spectroscopy of Water with High Spatial Resolution
journal, July 2018

Precision neutron diffraction structure determination of protein and nucleic acid components. I. Crystal and molecular structure of the amino acid L-alanine
journal, April 1972

Vibrational and valence aloof beam EELS: A potential tool for nondestructive characterization of nanoparticle surfaces
journal, September 2017

Identification of individual isotopes in a polymer blend using tip enhanced Raman spectroscopy: Identification of individual isotopes in a polymer blend
journal, March 2015

Uncertainties in Scaling Factors for ab Initio Vibrational Frequencies
journal, September 2005

The effect of pressure on the crystal structure of l-alanine
journal, January 2010

Towards weighing individual atoms by high-angle scattering of electrons
journal, April 2015

Transparent Window Vibrational Probes for the Characterization of Proteins With High Structural and Temporal Resolution
journal, January 2017

The infrared absorption of amino acid side chains
journal, January 2000

Advances in molecular quantum chemistry contained in the Q-Chem 4 program package
journal, September 2014

Structure and Vibrational Spectra of the Zwitterion l -Alanine in the Presence of Explicit Water Molecules: A Density Functional Analysis
journal, July 1998

The 3.8 A resolution cryo-EM structure of Zika virus
journal, March 2016

Two-dimensional IR spectroscopy and isotope labeling defines the pathway of amyloid formation with residue-specific resolution
journal, April 2009

Mapping vibrational surface and bulk modes in a single nanocube
journal, March 2017

Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms
journal, June 2003

Damage-free vibrational spectroscopy of biological materials in the electron microscope
journal, March 2016

Electron-Beam Mapping of Vibrational Modes with Nanometer Spatial Resolution
journal, December 2016

Development of electron energy-loss spectroscopy in the biological sciences
journal, January 2012

Temperature Measurement by a Nanoscale Electron Probe Using Energy Gain and Loss Spectroscopy
journal, March 2018

Emerging Electron Microscopy Techniques for Probing Functional Interfaces in Energy Materials
journal, October 2019

Spectrally tunable infrared plasmonic F,Sn:In ₂ O ₃ nanocrystal cubes
journal, January 2020

Far-field midinfrared superresolution imaging and spectroscopy of single high aspect ratio gold nanowires
journal, January 2020

Polarization Selectivity in Vibrational Electron-Energy-Loss Spectroscopy
journal, December 2019

Efficient and Versatile Model for Vibrational STEM-EELS
journal, January 2020

Isotopic compositions, nitrogen functional chemistry, and low‐loss electron spectroscopy of complex organic aggregates at the nanometer scale in the carbonaceous chondrite Renazzo
journal, October 2019