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Title: Dichotomous development of the gut microbiome in preterm infants

Abstract

Preterm infants are at risk of developing intestinal dysbiosis with an increased proportion of Gammaproteobacteria. In this study, we sought the clinical determinants of the relative abundance of feces-associated Gammaproteobacteria in very low birth weight (VLBW) infants. Fecal microbiome was characterized at ≤ 2 weeks and during the 3rd and 4th weeks after birth, by 16S rRNA amplicon sequencing. Maternal and infant clinical characteristics were extracted from electronic medical records. Data were analyzed by linear mixed modeling and linear regression. Clinical data and fecal microbiome profiles of 45 VLBW infants (gestational age 27.9 ± 2.2 weeks; birth weight 1126 ± 208 g) were studied. Three stool samples were analyzed for each infant at mean postnatal ages of 9.9 ± 3, 20.7 ± 4.1, and 29.4 ± 4.9 days. The average relative abundance of Gammaproteobacteria was 42.5% (0–90%) at ≤ 2 weeks, 69.7% (29.9–86.9%) in the 3rd, and 75.5% (54.5–86%) in the 4th week (p < 0.001). Hierarchical and K-means clustering identified two distinct subgroups: cluster 1 started with comparatively low abundance that increased with time, whereas cluster 2 began with a greater abundance at ≤ 2 weeks (p < 0.001) that decreased over time. Both groups resembled each other bymore » the 3rd week. Single variants of Klebsiella and Staphylococcus described variance in community structure between clusters and were shared between all infants, suggesting a common, hospital-derived source. Fecal Gammaproteobacteria was positively associated with vaginal delivery and antenatal steroids. We detected a dichotomy in gut microbiome assembly in preterm infants: some preterm infants started with low relative gammaproteobacterial abundance in stool that increased as a function of postnatal age, whereas others began with and maintained high abundance. Vaginal birth and antenatal steroids were identified as predictors of Gammaproteobacteria abundance in the early (≤ 2 weeks) and later (3rd and 4th weeks) stool samples, respectively. These findings are important in understanding the development of the gut microbiome in premature infants.« less

Authors:
 [1];  [2];  [3];  [4];  [1];  [5];  [6]
  1. Univ. of South Florida, Tampa, FL (United States). Dept. of Pediatrics. Morsani College of Medicine
  2. Univ. of South Florida, Tampa, FL (United States). Dept. of Pediatrics. Morsani College of Medicine. College of Nursing
  3. Univ. of South Florida, Tampa, FL (United States). College of Nursing
  4. Univ. of Chicago, IL (United States). Interdisciplinary Scientist Training Program. Microbiome Center. Dept. of Surgery
  5. Univ. of Chicago, IL (United States). Microbiome Center. Dept. of Surgery; Argonne National Lab. (ANL), Argonne, IL (United States)
  6. Univ. of South Florida, Tampa, FL (United States). Dept. of Pediatrics. Morsani College of Medicine; Johns Hopkins Univ., Baltimore, MD (United States). Dept. of Pediatrics
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of South Florida, Tampa, FL (United States); Univ. of Chicago, IL (United States); Johns Hopkins Univ., Baltimore, MD (United States)
Sponsoring Org.:
USDOE; National Inst. of Health (NIH) (United States)
OSTI Identifier:
1493912
Grant/Contract Number:  
AC02-06CH11357; NR015446; HL124078; HL133022; T32GM007281
Resource Type:
Accepted Manuscript
Journal Name:
Microbiome
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2049-2618
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; very low birth weight infant; Gammaproteobacteria; dysbiosis

Citation Formats

Ho, Thao T. B., Groer, Maureen W., Kane, Bradley, Yee, Alyson L., Torres, Benjamin A., Gilbert, Jack A., and Maheshwari, Akhil. Dichotomous development of the gut microbiome in preterm infants. United States: N. p., 2018. Web. doi:10.1186/s40168-018-0547-8.
Ho, Thao T. B., Groer, Maureen W., Kane, Bradley, Yee, Alyson L., Torres, Benjamin A., Gilbert, Jack A., & Maheshwari, Akhil. Dichotomous development of the gut microbiome in preterm infants. United States. doi:10.1186/s40168-018-0547-8.
Ho, Thao T. B., Groer, Maureen W., Kane, Bradley, Yee, Alyson L., Torres, Benjamin A., Gilbert, Jack A., and Maheshwari, Akhil. Wed . "Dichotomous development of the gut microbiome in preterm infants". United States. doi:10.1186/s40168-018-0547-8. https://www.osti.gov/servlets/purl/1493912.
@article{osti_1493912,
title = {Dichotomous development of the gut microbiome in preterm infants},
author = {Ho, Thao T. B. and Groer, Maureen W. and Kane, Bradley and Yee, Alyson L. and Torres, Benjamin A. and Gilbert, Jack A. and Maheshwari, Akhil},
abstractNote = {Preterm infants are at risk of developing intestinal dysbiosis with an increased proportion of Gammaproteobacteria. In this study, we sought the clinical determinants of the relative abundance of feces-associated Gammaproteobacteria in very low birth weight (VLBW) infants. Fecal microbiome was characterized at ≤ 2 weeks and during the 3rd and 4th weeks after birth, by 16S rRNA amplicon sequencing. Maternal and infant clinical characteristics were extracted from electronic medical records. Data were analyzed by linear mixed modeling and linear regression. Clinical data and fecal microbiome profiles of 45 VLBW infants (gestational age 27.9 ± 2.2 weeks; birth weight 1126 ± 208 g) were studied. Three stool samples were analyzed for each infant at mean postnatal ages of 9.9 ± 3, 20.7 ± 4.1, and 29.4 ± 4.9 days. The average relative abundance of Gammaproteobacteria was 42.5% (0–90%) at ≤ 2 weeks, 69.7% (29.9–86.9%) in the 3rd, and 75.5% (54.5–86%) in the 4th week (p < 0.001). Hierarchical and K-means clustering identified two distinct subgroups: cluster 1 started with comparatively low abundance that increased with time, whereas cluster 2 began with a greater abundance at ≤ 2 weeks (p < 0.001) that decreased over time. Both groups resembled each other by the 3rd week. Single variants of Klebsiella and Staphylococcus described variance in community structure between clusters and were shared between all infants, suggesting a common, hospital-derived source. Fecal Gammaproteobacteria was positively associated with vaginal delivery and antenatal steroids. We detected a dichotomy in gut microbiome assembly in preterm infants: some preterm infants started with low relative gammaproteobacterial abundance in stool that increased as a function of postnatal age, whereas others began with and maintained high abundance. Vaginal birth and antenatal steroids were identified as predictors of Gammaproteobacteria abundance in the early (≤ 2 weeks) and later (3rd and 4th weeks) stool samples, respectively. These findings are important in understanding the development of the gut microbiome in premature infants.},
doi = {10.1186/s40168-018-0547-8},
journal = {Microbiome},
number = ,
volume = 6,
place = {United States},
year = {2018},
month = {9}
}

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