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Title: Metagenomic analysis of basal ice from an Alaskan glacier

Abstract

Background Glaciers cover ~ 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska. Results DNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome. Conclusion Our results present the first metagenomic assembly and bacterial draft genomes for a subglacial environment. These results extend our understanding of the chemical and biological processes in subglacial environments critically influenced by global climate change.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [4]
  1. Tsinghua Univ., Beijing (China). School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Lab. of Bioinformatics
  2. Texas A & M Univ., College Station, TX (United States). College of Geosciences
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division; Univ. of Chicago, IL (United States). Microbiome Center, Dept. of Surgery
  4. Tsinghua Univ., Beijing (China). School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Lab. of Bioinformatics
  5. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division; Univ. of Chicago, IL (United States). Microbiome Center, Dept. of Surgery; Marine Biological Lab., Woods Hold, MA (United States). Microbiome Center
  6. Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science, Biodiversity Inst.
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE; Ministry of Science and Technology of the People's Republic of China; National Natural Science Foundation of China (NNSFC); National Aeronautic and Space Administration (NASA)
OSTI Identifier:
1493906
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Microbiome
Additional Journal Information:
Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2049-2618
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 58 GEOSCIENCES; 54 ENVIRONMENTAL SCIENCES; Microbiome; Metagenomics; Glacier; Basal ice layer

Citation Formats

Kayani, Masood ur Rehman, Doyle, Shawn M., Sangwan, Naseer, Wang, Guanqun, Gilbert, Jack A., Christner, Brent C., and Zhu, Ting F. Metagenomic analysis of basal ice from an Alaskan glacier. United States: N. p., 2018. Web. doi:10.1186/s40168-018-0505-5.
Kayani, Masood ur Rehman, Doyle, Shawn M., Sangwan, Naseer, Wang, Guanqun, Gilbert, Jack A., Christner, Brent C., & Zhu, Ting F. Metagenomic analysis of basal ice from an Alaskan glacier. United States. doi:10.1186/s40168-018-0505-5.
Kayani, Masood ur Rehman, Doyle, Shawn M., Sangwan, Naseer, Wang, Guanqun, Gilbert, Jack A., Christner, Brent C., and Zhu, Ting F. Thu . "Metagenomic analysis of basal ice from an Alaskan glacier". United States. doi:10.1186/s40168-018-0505-5. https://www.osti.gov/servlets/purl/1493906.
@article{osti_1493906,
title = {Metagenomic analysis of basal ice from an Alaskan glacier},
author = {Kayani, Masood ur Rehman and Doyle, Shawn M. and Sangwan, Naseer and Wang, Guanqun and Gilbert, Jack A. and Christner, Brent C. and Zhu, Ting F.},
abstractNote = {Background Glaciers cover ~ 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska. Results DNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome. Conclusion Our results present the first metagenomic assembly and bacterial draft genomes for a subglacial environment. These results extend our understanding of the chemical and biological processes in subglacial environments critically influenced by global climate change.},
doi = {10.1186/s40168-018-0505-5},
journal = {Microbiome},
number = 1,
volume = 6,
place = {United States},
year = {2018},
month = {7}
}

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