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Title: Soil exchange rates of COS and CO 18O differ with the diversity of microbial communities and their carbonic anhydrase enzymes

Abstract

Here, differentiating the contributions of photosynthesis and respiration to the global carbon cycle is critical for improving predictive climate models. Carbonic anhydrase (CA) activity in leaves is responsible for the largest biosphere-atmosphere trace gas fluxes of carbonyl sulfide (COS) and the oxygen-18 isotopologue of carbon dioxide (CO 18O) that both reflect gross photosynthetic rates. However, CA activity also occurs in soils and will be a source of uncertainty in the use of COS and CO 18O as carbon cycle tracers until process-based constraints are improved. In this study, we measured COS and CO 18O exchange rates and estimated the corresponding CA activity in soils from a range of biomes and land use types. Soil CA activity was not uniform for COS and CO 2, and patterns of divergence were related to microbial community composition and CA gene expression patterns. In some cases, the same microbial taxa and CA classes catalyzed both COS and CO 2 reactions in soil, but in other cases the specificity towards the two substrates differed markedly. CA activity for COS was related to fungal taxa and β-D-CA expression, whereas CA activity for CO 2 was related to algal and bacterial taxa and α-CA expression. This studymore » integrates gas exchange measurements, enzyme activity models, and characterization of soil taxonomic and genetic diversity to build connections between CA activity and the soil microbiome. Importantly, our results identify kinetic parameters to represent soil CA activity during application of COS and CO18O as carbon cycle tracers.« less

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3]; ORCiD logo [4];  [2];  [5];  [6];  [7];  [8];  [9];  [10];  [7];  [8]
  1. Stanford Univ., Stanford, CA (United States); Univ. of Arizona, Tucson, AZ (United States)
  2. INRA/Bordeaux Science Agro, Bordeaux (France)
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  4. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  5. Univ. of Bonn, Bonn (Germany); McGill Univ., Montreal, QC (Canada)
  6. Univ. of Arizona, Tucson, AZ (United States)
  7. Carnegie Institution for Science, Stanford, CA (United States)
  8. Stanford Univ., Stanford, CA (United States)
  9. Univ. of Massachusetts, Amherst, MA (United States)
  10. Forschungszentrum Julich, Julich (Germany)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1493420
Alternate Identifier(s):
OSTI ID: 1560590
Grant/Contract Number:  
1331214; CSP 2033; AC02-76SF00515; AC02–05CH11231; 338264; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Volume: 13; Journal Issue: 2; Journal ID: ISSN 1751-7362
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Meredith, Laura K., Ogée, Jérôme, Boye, Kristin, Singer, Esther, Wingate, Lisa, von Sperber, Christian, Sengupta, Aditi, Whelan, Mary, Pang, Erin, Keiluweit, Marco, Brüggemann, Nicolas, Berry, Joe A., and Welander, Paula V. Soil exchange rates of COS and CO18O differ with the diversity of microbial communities and their carbonic anhydrase enzymes. United States: N. p., 2018. Web. doi:10.1038/s41396-018-0270-2.
Meredith, Laura K., Ogée, Jérôme, Boye, Kristin, Singer, Esther, Wingate, Lisa, von Sperber, Christian, Sengupta, Aditi, Whelan, Mary, Pang, Erin, Keiluweit, Marco, Brüggemann, Nicolas, Berry, Joe A., & Welander, Paula V. Soil exchange rates of COS and CO18O differ with the diversity of microbial communities and their carbonic anhydrase enzymes. United States. doi:10.1038/s41396-018-0270-2.
Meredith, Laura K., Ogée, Jérôme, Boye, Kristin, Singer, Esther, Wingate, Lisa, von Sperber, Christian, Sengupta, Aditi, Whelan, Mary, Pang, Erin, Keiluweit, Marco, Brüggemann, Nicolas, Berry, Joe A., and Welander, Paula V. Thu . "Soil exchange rates of COS and CO18O differ with the diversity of microbial communities and their carbonic anhydrase enzymes". United States. doi:10.1038/s41396-018-0270-2. https://www.osti.gov/servlets/purl/1493420.
@article{osti_1493420,
title = {Soil exchange rates of COS and CO18O differ with the diversity of microbial communities and their carbonic anhydrase enzymes},
author = {Meredith, Laura K. and Ogée, Jérôme and Boye, Kristin and Singer, Esther and Wingate, Lisa and von Sperber, Christian and Sengupta, Aditi and Whelan, Mary and Pang, Erin and Keiluweit, Marco and Brüggemann, Nicolas and Berry, Joe A. and Welander, Paula V.},
abstractNote = {Here, differentiating the contributions of photosynthesis and respiration to the global carbon cycle is critical for improving predictive climate models. Carbonic anhydrase (CA) activity in leaves is responsible for the largest biosphere-atmosphere trace gas fluxes of carbonyl sulfide (COS) and the oxygen-18 isotopologue of carbon dioxide (CO18O) that both reflect gross photosynthetic rates. However, CA activity also occurs in soils and will be a source of uncertainty in the use of COS and CO18O as carbon cycle tracers until process-based constraints are improved. In this study, we measured COS and CO18O exchange rates and estimated the corresponding CA activity in soils from a range of biomes and land use types. Soil CA activity was not uniform for COS and CO2, and patterns of divergence were related to microbial community composition and CA gene expression patterns. In some cases, the same microbial taxa and CA classes catalyzed both COS and CO2 reactions in soil, but in other cases the specificity towards the two substrates differed markedly. CA activity for COS was related to fungal taxa and β-D-CA expression, whereas CA activity for CO2 was related to algal and bacterial taxa and α-CA expression. This study integrates gas exchange measurements, enzyme activity models, and characterization of soil taxonomic and genetic diversity to build connections between CA activity and the soil microbiome. Importantly, our results identify kinetic parameters to represent soil CA activity during application of COS and CO18O as carbon cycle tracers.},
doi = {10.1038/s41396-018-0270-2},
journal = {The ISME Journal},
number = 2,
volume = 13,
place = {United States},
year = {2018},
month = {9}
}

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Figures / Tables:

Fig. 1 Fig. 1: Variation in soil exchange rates of COS and CO18O with biome and land use. a COS net soil exchange (colored bars) and partitioned contributions of COS production (white bar upper) and consumption (white bar lower). b Fraction of atmospheric CO2 molecules that equilibrated oxygen isotopes with soil watermore » within a 3-min residence time of the dynamic chamber measurement. Whiskers represent SD. Biome indicated by color key and agricultural sites denoted by symbol« less

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