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Title: Over-Expression of a Maize N-Acetylglutamate Kinase Gene ( ZmNAGK) Improves Drought Tolerance in Tobacco

Abstract

Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize ( Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H 2O 2. The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. Here, these results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [1]
  1. Nanjing Agricultural Univ., Nanjing (China)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1492316
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; drought stress; ZmNAGK; tobacco; abiotic stress; arginine; NO

Citation Formats

Liu, Weijuan, Xiang, Yang, Zhang, Xiaoyun, Han, Gaoqiang, Sun, Xiujuan, Sheng, Yu, Yan, Jingwei, Scheller, Henrik Vibe, and Zhang, Aying. Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco. United States: N. p., 2019. Web. doi:10.3389/fpls.2018.01902.
Liu, Weijuan, Xiang, Yang, Zhang, Xiaoyun, Han, Gaoqiang, Sun, Xiujuan, Sheng, Yu, Yan, Jingwei, Scheller, Henrik Vibe, & Zhang, Aying. Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco. United States. doi:10.3389/fpls.2018.01902.
Liu, Weijuan, Xiang, Yang, Zhang, Xiaoyun, Han, Gaoqiang, Sun, Xiujuan, Sheng, Yu, Yan, Jingwei, Scheller, Henrik Vibe, and Zhang, Aying. Fri . "Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco". United States. doi:10.3389/fpls.2018.01902. https://www.osti.gov/servlets/purl/1492316.
@article{osti_1492316,
title = {Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco},
author = {Liu, Weijuan and Xiang, Yang and Zhang, Xiaoyun and Han, Gaoqiang and Sun, Xiujuan and Sheng, Yu and Yan, Jingwei and Scheller, Henrik Vibe and Zhang, Aying},
abstractNote = {Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H2O2. The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. Here, these results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.},
doi = {10.3389/fpls.2018.01902},
journal = {Frontiers in Plant Science},
number = ,
volume = 9,
place = {United States},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Figures / Tables:

FIGURE 1 FIGURE 1: Alignment and phylogenetic analysis of ZmNAGK with NAGKs from different species. (A) ZmNAGK encodes a protein of 345 amino acid residues and contains an AA-kinase domain. (B) The amino-acid sequences of NAGKs from Arabidopsis thaliana, Brachypodium distachyon, Nicotiana tabacum, Oryza sativa, Setaria italica, Sorghum bicolor, and Zea maysmore » were aligned. Identical and similar residues were shaded in black and gray, respectively. The putative plastid-target sequence, which was predicted by the iPSORT (http://ipsort.hgc.jp/) programs are boxed. (C) Phylogeny tree of NAGKs was calculated in the MEGA 6.0 program. The phylogenetic relationship between the examined sequences was performed using the Neighbor-Joining method with p-distance correction. Bootstrap values were derived from 1000 replicate runs. The accession numbers: AtNAGK (NP_191315.1), BdNAGK-like (XP_003570025.1), NtNAGK-like (XP_016482712.1), OsNAGK1 (XP_015633506.1), OsNAGK2 (BAD88532.1), SiNAGK-like (XP_004976436.1), SbNAGK-like (XP_002446901.2), ZmNAGK (XP_008668008.1).« less

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Works referenced in this record:

Nitric Oxide Is Required for Melatonin-Enhanced Tolerance against Salinity Stress in Rapeseed (Brassica napus L.) Seedlings
journal, June 2018

  • Zhao, Gan; Zhao, Yingying; Yu, Xiuli
  • International Journal of Molecular Sciences, Vol. 19, Issue 7
  • DOI: 10.3390/ijms19071912

Mechanisms for nitric oxide synthesis in plants
journal, December 2005

  • Crawford, Nigel M.
  • Journal of Experimental Botany, Vol. 57, Issue 3
  • DOI: 10.1093/jxb/erj050

Arginine and nitrogen storage
journal, December 2008

  • Llácer, José L.; Fita, Ignacio; Rubio, Vicente
  • Current Opinion in Structural Biology, Vol. 18, Issue 6
  • DOI: 10.1016/j.sbi.2008.11.002

Nitric oxide signalling in plants
journal, July 2003


The PII Signal Transduction Protein of Arabidopsis thaliana Forms an Arginine-regulated Complex with Plastid N -Acetyl Glutamate Kinase
journal, December 2005

  • Chen, Yan M.; Ferrar, Tony S.; Lohmeir-Vogel, Elke
  • Journal of Biological Chemistry, Vol. 281, Issue 9
  • DOI: 10.1074/jbc.M510945200

NO is involved in spermidine-induced drought tolerance in white clover via activation of antioxidant enzymes and genes
journal, September 2015


Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance
journal, November 2003


Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass
journal, July 2008

  • Xu, Z.; Zhou, G.
  • Journal of Experimental Botany, Vol. 59, Issue 12
  • DOI: 10.1093/jxb/ern185

Genes, enzymes and regulation of arginine biosynthesis in plants
journal, August 2005


Plant Productivity and Environment
journal, October 1982


OsDMI3 Is a Novel Component of Abscisic Acid Signaling in the Induction of Antioxidant Defense in Leaves of Rice
journal, November 2012

  • Shi, Ben; Ni, Lan; Zhang, Aying
  • Molecular Plant, Vol. 5, Issue 6
  • DOI: 10.1093/mp/sss068

Arginine Metabolism in Developing Soybean Cotyledons: III. Utilization
journal, September 1989

  • Micallef, Barry J.; Shelp, Barry J.
  • Plant Physiology, Vol. 91, Issue 1
  • DOI: 10.1104/pp.91.1.170

Superoxide Dismutases: I. Occurrence in Higher Plants
journal, February 1977

  • Giannopolitis, Constantine N.; Ries, Stanley K.
  • Plant Physiology, Vol. 59, Issue 2
  • DOI: 10.1104/pp.59.2.309

Overexpression of an MYB-Related Gene FvMYB1 from Fraxinus velutina Increases Tolerance to Salt Stress in Transgenic Tobacco
journal, March 2016


Abiotic stress response in plants: When post-transcriptional and post-translational regulations control transcription
journal, April 2008


TaASR1 , a transcription factor gene in wheat, confers drought stress tolerance in transgenic tobacco : The role of TaASR1 in drought stress
journal, February 2013

  • Hu, Wei; Huang, Chao; Deng, Xiaomin
  • Plant, Cell & Environment, Vol. 36, Issue 8
  • DOI: 10.1111/pce.12074

Global Synthesis of Drought Effects on Maize and Wheat Production
journal, May 2016


Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants
journal, November 2016

  • Kudo, Madoka; Kidokoro, Satoshi; Yoshida, Takuya
  • Plant Biotechnology Journal, Vol. 15, Issue 4
  • DOI: 10.1111/pbi.12644

Nitric Oxide Mediates Brassinosteroid-Induced ABA Biosynthesis Involved in Oxidative Stress Tolerance in Maize Leaves
journal, December 2010

  • Zhang, A.; Zhang, J.; Zhang, J.
  • Plant and Cell Physiology, Vol. 52, Issue 1
  • DOI: 10.1093/pcp/pcq187

Purification and characterization of N-acetylglutamate 5-phosphotransferase from pea (Pisum sativum) cotyledons
journal, April 1981

  • McKay, G.; Shargool, P. D.
  • Biochemical Journal, Vol. 195, Issue 1
  • DOI: 10.1042/bj1950071

Exogenously Applied Nitric Oxide Enhances the Drought Tolerance in Fine Grain Aromatic Rice ( Oryza sativa L.)
journal, August 2009


MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize
journal, August 2013

  • Zhu, Yuan; Zuo, Mingxing; Liang, Yali
  • Journal of Experimental Botany, Vol. 64, Issue 12
  • DOI: 10.1093/jxb/ert215

Functional roles of the pepper MLO protein gene, CaMLO2, in abscisic acid signaling and drought sensitivity
journal, November 2013


Polyamines: ubiquitous polycations with unique roles in growth and stress responses
journal, October 2009

  • Takahashi, Taku; Kakehi, Jun-Ichi
  • Annals of Botany, Vol. 105, Issue 1
  • DOI: 10.1093/aob/mcp259

Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber
journal, April 2009

  • Xia, Xiao-Jian; Wang, Yan-Jie; Zhou, Yan-Hong
  • Plant Physiology, Vol. 150, Issue 2
  • DOI: 10.1104/pp.109.138230

Exogenous nitric oxide donor and arginine provide protection against short-term drought stress in wheat seedlings
journal, May 2018

  • Hasanuzzaman, Mirza; Nahar, Kamrun; Rahman, Anisur
  • Physiology and Molecular Biology of Plants, Vol. 24, Issue 6
  • DOI: 10.1007/s12298-018-0531-6

Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana
journal, January 2012


Essential Role of Tissue-Specific Proline Synthesis and Catabolism in Growth and Redox Balance at Low Water Potential
journal, July 2011

  • Sharma, Sandeep; Villamor, Joji Grace; Verslues, Paul E.
  • Plant Physiology, Vol. 157, Issue 1
  • DOI: 10.1104/pp.111.183210

Drought tolerance improvement in crop plants: An integrated view from breeding to genomics
journal, January 2008

  • Cattivelli, Luigi; Rizza, Fulvia; Badeck, Franz-W.
  • Field Crops Research, Vol. 105, Issue 1-2
  • DOI: 10.1016/j.fcr.2007.07.004

Understanding regulatory networks and engineering for enhanced drought tolerance in plants
journal, April 2006


Nitric oxide production in tobacco leaf cells: a generalized stress response?
journal, November 2003


Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations
journal, April 2005


Physiological implications of arginine metabolism in plants
journal, July 2015

  • Winter, Gudrun; Todd, Christopher D.; Trovato, Maurizio
  • Frontiers in Plant Science, Vol. 6
  • DOI: 10.3389/fpls.2015.00534

Nitric Oxide Reduces Aluminum Toxicity by Preventing Oxidative Stress in the Roots of Cassia tora L.
journal, December 2005

  • Wang, You-Sheng; Yang, Zhi-Min
  • Plant and Cell Physiology, Vol. 46, Issue 12
  • DOI: 10.1093/pcp/pci202

Interaction of N-Acetylglutamate Kinase with a PII-Like Protein in Rice
journal, December 2004

  • Sugiyama, Kenjiro; Hayakawa, Toshihiko; Kudo, Toru
  • Plant and Cell Physiology, Vol. 45, Issue 12
  • DOI: 10.1093/pcp/pch199

Over-expression of a tomato N-acetyl-L-glutamate synthase gene (SlNAGS1) in Arabidopsis thaliana results in high ornithine levels and increased tolerance in salt and drought stresses
journal, April 2009

  • Kalamaki, Mary S.; Alexandrou, Dimitris; Lazari, Diamanto
  • Journal of Experimental Botany, Vol. 60, Issue 6
  • DOI: 10.1093/jxb/erp072

Ornithine biosynthesis, and arginine biosynthesis and degradation in plant cells
journal, January 1988


New Insights into Nitric Oxide Signaling in Plants
journal, June 2008


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