Automated cassette-based production of high specific activity [203/212Pb]peptide-based theranostic radiopharmaceuticals for image-guided radionuclide therapy for cancer

Li, Mengshi; Zhang, Xiuli; Quinn, Thomas P.; Lee, Dongyoul; Liu, Dijie; Kunkel, Falk; Zimmerman, Brian E.; McAlister, Daniel; Olewein, Keith; Menda, Yusuf; Mirzadeh, Saed; Copping, Roy; Johnson, Frances L.; Schultz, Michael K.

doi:10.1016/j.apradiso.2017.05.006

Title: Automated cassette-based production of high specific activity [^203/212Pb]peptide-based theranostic radiopharmaceuticals for image-guided radionuclide therapy for cancer

Journal Article · 10 May 2017 · Applied Radiation and Isotopes

DOI: https://doi.org/10.1016/j.apradiso.2017.05.006 · OSTI ID:1491308

Li, Mengshi ^[1]; Zhang, Xiuli ^[2]; Quinn, Thomas P. ^[2]; Lee, Dongyoul ^[1]; Liu, Dijie ^[3]; Kunkel, Falk ^[4]; Zimmerman, Brian E. ^[5]; McAlister, Daniel ^[6]; Olewein, Keith ^[7]; Menda, Yusuf ^[8];

^[9];

^[9]; Johnson, Frances L. ^[10]; Schultz, Michael K. ^[11]

Univ. of Iowa, Iowa City, IA (United States). Interdisciplinary Graduate Program in Human Toxicology
Univ. of Missouri, Columbia, MO (United States). Dept. of Biochemistry
Univ. of Iowa, Iowa City, IA (United States). Stead Family Dept. of Pediatrics
Eckert & Ziegler Radiopharma GmbH, Berlin (Germany)
National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States)
Eichrom Technologies, LLC, Lisle, IL (United States)
Lantheus Medical Imaging North Billerica, MA (United States)
Univ. of Iowa, Iowa City, IA (United States). Dept. of Radiology
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Viewpoint Molecular Targeting, LLC, Coralville, IA (United States); Univ. of Iowa, Iowa City, IA (United States). Carver College of Medicine, Dept. of Internal Medicine
Univ. of Iowa, Iowa City, IA (United States). Interdisciplinary Graduate Program in Human Toxicology; Univ. of Iowa, Iowa City, IA (United States). Stead Family Dept. of Pediatrics; Univ. of Iowa, Iowa City, IA (United States). Dept. of Radiology; Viewpoint Molecular Targeting, LLC, Coralville, IA (United States); Univ. of Iowa, Iowa City, IA (United States). Carver College of Medicine, Dept. of Radiation Oncology; Univ. of Iowa, Iowa City, IA (United States). Dept. of Chemistry

A method for preparation of Pb-212 and Pb-203 labeled chelator-modified peptide-based radiopharmaceuticals for cancer imaging and radionuclide therapy has been developed and adapted for automated clinical production. Pre-concentration and isolation of radioactive Pb2+ from interfering metals in dilute hydrochloric acid was optimized using a commercially-available Pb-specific chromatography resin packed in disposable plastic columns. The pre-concentrated radioactive Pb2+ is eluted in NaOAc buffer directly to the reaction vessel containing chelator-modified peptides. Radiolabeling was found to proceed efficiently at 85 °C (45 min; pH 5.5). The specific activity of radiolabeled conjugates was optimized by separation of radiolabeled conjugates from unlabeled peptide via HPLC. Preservation of bioactivity was confirmed by in vivo biodistribution of Pb-203 and Pb-212 labeled peptides in melanoma-tumor-bearing mice. The approach has been found to be robustly adaptable to automation and a cassette-based fluid-handling system (Modular Lab Pharm Tracer) has been customized for clinical radiopharmaceutical production. Our findings demonstrate that the Pb-203/Pb-212 combination is a promising elementally-matched radionuclide pair for image-guided radionuclide therapy for melanoma, neuroendocrine tumors, and potentially other cancers.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1491308

Journal Information:: Applied Radiation and Isotopes, Vol. 127, Issue C; ISSN 0969-8043

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 29 works

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