Programming molecular topologies from single-stranded nucleic acids

Qi, Xiaodong; Zhang, Fei; Su, Zhaoming; Jiang, Shuoxing; Han, Dongran; Ding, Baoquan; Liu, Yan; Chiu, Wah; Yin, Peng; Yan, Hao

doi:10.1038/s41467-018-07039-7

Title: Programming molecular topologies from single-stranded nucleic acids

Journal Article · 01 November 2018 · Nature Communications

DOI: https://doi.org/10.1038/s41467-018-07039-7 · OSTI ID:1490645

^[1]; Zhang, Fei ^[1]; Su, Zhaoming ^[2];

^[1]; Han, Dongran ^[3]; Ding, Baoquan ^[4];

^[1]; Chiu, Wah ^[2];

^[3]; Yan, Hao ^[1]

Arizona State Univ., Tempe, AZ (United States)
Stanford Univ., Stanford, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)
Harvard Medical School, Boston, MA (United States); Harvard Univ., Boston, MA (United States)
National Center for Nanoscience and Technology, Beijing (China); Univ. of Chinese Academy of Sciences, Beijing (China)

Molecular knots represent one of the most extraordinary topological structures in biological polymers. Creating highly knotted nanostructures with well-defined and sophisticated geometries and topologies remains challenging. Here, we demonstrate a general strategy to design and construct highly knotted nucleic acid nanostructures, each weaved from a single-stranded DNA or RNA chain by hierarchical folding in a prescribed order. Sets of DNA and RNA knots of two- or three-dimensional shapes have been designed and constructed (ranging from 1700 to 7500 nucleotides), and they exhibit complex topological features, with high crossing numbers (from 9 up to 57). These single-stranded DNA/RNA knots can be replicated and amplified enzymatically in vitro and in vivo. Furthermore, this work establishes a general platform for constructing nucleic acid nanostructures with complex molecular topologies.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1490645

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 9; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Next-Generation Digital Information Storage in DNA Church, G. M.; Gao, Y.; Kosuri, S. Science, Vol. 337, Issue 6102 https://doi.org/10.1126/science.1226355	journal	August 2012
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