skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Universal nanothin silk coatings via controlled spidroin self-assembly

Abstract

Biomimetic spidroin self-assembly allows formation of substrate-independent one-pot nanothin silk coatings.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [2]
  1. Department of Chemical & Biological Engineering, Rensselaer Polytechnic Institute, Troy, USA
  2. Department of Materials Science and Engineering, Department of Bioengineering, University of California – Berkeley, Berkeley, USA
  3. Department for Biomaterials, University of Bayreuth, 95440 Bayreuth, Germany
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1490499
Grant/Contract Number:  
SC0012704
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Biomaterials Science
Additional Journal Information:
Journal Name: Biomaterials Science Journal Volume: 7 Journal Issue: 2; Journal ID: ISSN 2047-4830
Publisher:
Royal Society of Chemistry (RSC)
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Zha, R. Helen, Delparastan, Peyman, Fink, Tanner D., Bauer, Joschka, Scheibel, Thomas, and Messersmith, Phillip B. Universal nanothin silk coatings via controlled spidroin self-assembly. United Kingdom: N. p., 2019. Web. doi:10.1039/C8BM01186A.
Zha, R. Helen, Delparastan, Peyman, Fink, Tanner D., Bauer, Joschka, Scheibel, Thomas, & Messersmith, Phillip B. Universal nanothin silk coatings via controlled spidroin self-assembly. United Kingdom. doi:10.1039/C8BM01186A.
Zha, R. Helen, Delparastan, Peyman, Fink, Tanner D., Bauer, Joschka, Scheibel, Thomas, and Messersmith, Phillip B. Tue . "Universal nanothin silk coatings via controlled spidroin self-assembly". United Kingdom. doi:10.1039/C8BM01186A.
@article{osti_1490499,
title = {Universal nanothin silk coatings via controlled spidroin self-assembly},
author = {Zha, R. Helen and Delparastan, Peyman and Fink, Tanner D. and Bauer, Joschka and Scheibel, Thomas and Messersmith, Phillip B.},
abstractNote = {Biomimetic spidroin self-assembly allows formation of substrate-independent one-pot nanothin silk coatings.},
doi = {10.1039/C8BM01186A},
journal = {Biomaterials Science},
number = 2,
volume = 7,
place = {United Kingdom},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1039/C8BM01186A

Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

An Engineered Spider Silk Protein Forms Microspheres
journal, June 2008

  • Slotta, Ute K.; Rammensee, Sebastian; Gorb, Stanislav
  • Angewandte Chemie International Edition, Vol. 47, Issue 24
  • DOI: 10.1002/anie.200800683

Spider Silk and Amyloid Fibrils: A Structural Comparison
journal, February 2007

  • Slotta, Ute; Hess, Simone; Spieß, Kristina
  • Macromolecular Bioscience, Vol. 7, Issue 2
  • DOI: 10.1002/mabi.200600201

Hierarchical structures made of proteins. The complex architecture of spider webs and their constituent silk proteins
journal, January 2010

  • Heim, Markus; Römer, Lin; Scheibel, Thomas
  • Chem. Soc. Rev., Vol. 39, Issue 1
  • DOI: 10.1039/B813273A

Elastin-like Polypeptide Linkers for Single-Molecule Force Spectroscopy
journal, May 2017


Changes in element composition along the spinning duct in a Nephila spider
journal, June 2001


Comparative studies of fibroins
journal, December 1960


Rheological characterization of hydrogels formed by recombinantly produced spider silk
journal, November 2005


Use of hydrogel coating to improve the performance of implanted glucose sensors
journal, March 2008


Injectable dynamic covalent hydrogels of boronic acid polymers cross-linked by bioactive plant-derived polyphenols
journal, January 2018

  • Huang, Zhuojun; Delparastan, Peyman; Burch, Patrick
  • Biomaterials Science, Vol. 6, Issue 9
  • DOI: 10.1039/C8BM00453F

Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings
journal, January 2009

  • Lee, Haeshin; Rho, Junsung; Messersmith, Phillip B.
  • Advanced Materials, Vol. 21, Issue 4
  • DOI: 10.1002/adma.200801222

Polymeric materials based on silk proteins
journal, September 2008


The role of proline in the elastic mechanism of hydrated spider silks
journal, June 2008

  • Savage, K. N.; Gosline, J. M.
  • Journal of Experimental Biology, Vol. 211, Issue 12
  • DOI: 10.1242/jeb.014225

Interactions of cells with silk surfaces
journal, January 2012

  • Leal-Egaña, Aldo; Scheibel, Thomas
  • Journal of Materials Chemistry, Vol. 22, Issue 29
  • DOI: 10.1039/c2jm31174g

Varying surface hydrophobicities of coatings made of recombinant spider silk proteins
journal, January 2012

  • Wohlrab, Stefanie; Spieß, Kristina; Scheibel, Thomas
  • Journal of Materials Chemistry, Vol. 22, Issue 41
  • DOI: 10.1039/c2jm35075k

Ten Years of Polydopamine: Current Status and Future Directions
journal, February 2018

  • Schanze, Kirk S.; Lee, Haeshin; Messersmith, Phillip B.
  • ACS Applied Materials & Interfaces, Vol. 10, Issue 9
  • DOI: 10.1021/acsami.8b02929

In vitro evaluation of the inflammatory potential of the silk fibroin
journal, September 1999


Surface properties of spider silk particles in solution
journal, January 2013

  • Helfricht, Nicolas; Klug, Maria; Mark, Andreas
  • Biomaterials Science, Vol. 1, Issue 11
  • DOI: 10.1039/c3bm60109a

Primary Structure Elements of Spider Dragline Silks and Their Contribution to Protein Solubility
journal, October 2004

  • Huemmerich, Daniel; Helsen, Christopher W.; Quedzuweit, Susanne
  • Biochemistry, Vol. 43, Issue 42
  • DOI: 10.1021/bi048983q

Assembly mechanism of recombinant spider silk proteins
journal, April 2008

  • Rammensee, S.; Slotta, U.; Scheibel, T.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 18
  • DOI: 10.1073/pnas.0709246105

Silk–Its Mysteries, How It Is Made, and How It Is Used
journal, September 2015


Micromechanical characterization of spider silk particles
journal, January 2013

  • Neubauer, Martin P.; Blüm, Claudia; Agostini, Elisa
  • Biomaterials Science, Vol. 1, Issue 11
  • DOI: 10.1039/c3bm60108k

The crystal structure of silk fibroins
journal, January 1956


Molecular Modeling of Spider Silk Elasticity
journal, December 1994


Surface-induced spreading phenomenon of protein clusters
journal, January 2009

  • Rabe, Michael; Verdes, Dorinel; Seeger, Stefan
  • Soft Matter, Vol. 5, Issue 5
  • DOI: 10.1039/b814053g

The elaborate structure of spider silk: Structure and function of a natural high performance fiber
journal, October 2008


Processing Conditions for the Formation of Spider Silk Microspheres
journal, May 2008


Structural Analysis of Spider Silk Films
journal, July 2006


Spider Silk: From Soluble Protein to Extraordinary Fiber
journal, May 2009

  • Heim, Markus; Keerl, David; Scheibel, Thomas
  • Angewandte Chemie International Edition, Vol. 48, Issue 20
  • DOI: 10.1002/anie.200803341

Understanding protein adsorption phenomena at solid surfaces
journal, February 2011

  • Rabe, Michael; Verdes, Dorinel; Seeger, Stefan
  • Advances in Colloid and Interface Science, Vol. 162, Issue 1-2
  • DOI: 10.1016/j.cis.2010.12.007

Elasticity, structure, and relaxation of extended proteins under force
journal, February 2013

  • Stirnemann, G.; Giganti, D.; Fernandez, J. M.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 10
  • DOI: 10.1073/pnas.1300596110

Spider Silk Coatings as a Bioshield to Reduce Periprosthetic Fibrous Capsule Formation
journal, January 2014

  • Zeplin, Philip H.; Maksimovikj, Nathalie C.; Jordan, Martin C.
  • Advanced Functional Materials, Vol. 24, Issue 18
  • DOI: 10.1002/adfm.201302813

Nanostructure and molecular mechanics of spider dragline silk protein assemblies
journal, June 2010

  • Keten, Sinan; Buehler, Markus J.
  • Journal of The Royal Society Interface, Vol. 7, Issue 53
  • DOI: 10.1098/rsif.2010.0149

Silk as a biomaterial
journal, August 2007


Functionalized silk-based biomaterials for bone formation
journal, January 2000


Liquid crystalline spinning of spider silk
journal, March 2001

  • Vollrath, Fritz; Knight, David P.
  • Nature, Vol. 410, Issue 6828
  • DOI: 10.1038/35069000

Mussel-Inspired Surface Chemistry for Multifunctional Coatings
journal, October 2007


The inflammatory responses to silk films in vitro and in vivo
journal, January 2005


Biomaterial Coatings by Stepwise Deposition of Silk Fibroin
journal, November 2005

  • Wang, Xianyan; Kim, Hyeon Joo; Xu, Peng
  • Langmuir, Vol. 21, Issue 24
  • DOI: 10.1021/la051862m

Nanomaterial Building Blocks Based on Spider Silk–Oligonucleotide Conjugates
journal, January 2014


Fiber Morphology of Spider Silk:  The Effects of Tensile Deformation
journal, May 1997

  • Grubb, David T.; Jelinski, Lynn W.
  • Macromolecules, Vol. 30, Issue 10
  • DOI: 10.1021/ma961293c

Single-blind, Randomised, Parallel Group Study of the Bard Biocath Catheter and a Silicone Elastomer Coated Catheter
journal, October 1991


Relationships between supercontraction and mechanical properties of spider silk
journal, November 2005

  • Liu, Yi; Shao, Zhengzhong; Vollrath, Fritz
  • Nature Materials, Vol. 4, Issue 12
  • DOI: 10.1038/nmat1534

The crystal structure of silk fibroin
journal, January 1955


Polymeric “smart” coatings to prevent foreign body response to implantable biosensors
journal, August 2013


Structural changes of thin films from recombinant spider silk proteins upon post-treatment
journal, September 2007


Silk protein-based hydrogels: Promising advanced materials for biomedical applications
journal, February 2016


New strategies to prevent catheter-associated urinary tract infections
journal, April 2012


From car parking to protein adsorption: an overview of sequential adsorption processes
journal, May 2000

  • Talbot, J.; Tarjus, G.; Van Tassel, P. R.
  • Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 165, Issue 1-3
  • DOI: 10.1016/S0927-7757(99)00409-4

Segmented nanofibers of spider dragline silk: Atomic force microscopy and single-molecule force spectroscopy
journal, April 2002

  • Oroudjev, E.; Soares, J.; Arcidiacono, S.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue Supplement 2
  • DOI: 10.1073/pnas.082526499

Polydopamine Surface Chemistry: A Decade of Discovery
journal, February 2018

  • Ryu, Ji Hyun; Messersmith, Phillip B.; Lee, Haeshin
  • ACS Applied Materials & Interfaces, Vol. 10, Issue 9
  • DOI: 10.1021/acsami.7b19865

Spider silk as a model biomaterial
journal, November 2005


Influence of repeat numbers on self-assembly rates of repetitive recombinant spider silk proteins
journal, June 2014

  • Humenik, Martin; Magdeburg, Michael; Scheibel, Thomas
  • Journal of Structural Biology, Vol. 186, Issue 3
  • DOI: 10.1016/j.jsb.2014.03.010

A Review of Organic and Inorganic Biomaterials for Neural Interfaces
journal, February 2014


The molecular structure of spider dragline silk: Folding and orientation of the protein backbone
journal, July 2002

  • van Beek, J. D.; Hess, S.; Vollrath, F.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 16
  • DOI: 10.1073/pnas.152162299

Using selected uniform cells in round shape with a micropipette to measure cell adhesion strength on silk fibroin-based materials
journal, December 2008


De novo engineering of reticular connective tissue in vivo by silk fibroin nonwoven materials
journal, May 2005


Three intrinsically unstructured mussel adhesive proteins, mfp-1, mfp-2, and mfp-3: Analysis by circular dichroism
journal, September 2012

  • Hwang, Dong Soo; Waite, J. Herbert
  • Protein Science, Vol. 21, Issue 11
  • DOI: 10.1002/pro.2147

Universal polymer coatings and their representative biomedical applications
journal, January 2015


Silk-inspired polymers and proteins
journal, July 2009

  • Hardy, John G.; Scheibel, Thomas R.
  • Biochemical Society Transactions, Vol. 37, Issue 4
  • DOI: 10.1042/BST0370677

Silk and Silk-Like Supramolecular Materials
journal, February 2018

  • Fink, Tanner D.; Zha, R. Helen
  • Macromolecular Rapid Communications, Vol. 39, Issue 17
  • DOI: 10.1002/marc.201700834

Transition to a β-Sheet-Rich Structure in Spidroin in Vitro:  The Effects of pH and Cations
journal, November 2004

  • Dicko, Cedric; Kenney, John M.; Knight, David
  • Biochemistry, Vol. 43, Issue 44
  • DOI: 10.1021/bi0483413

Controllable cell adhesion, growth and orientation on layered silk protein films
journal, January 2013

  • Bauer, Felix; Wohlrab, Stefanie; Scheibel, Thomas
  • Biomaterials Science, Vol. 1, Issue 12
  • DOI: 10.1039/c3bm60114e

Interactions of Fibroblasts with Different Morphologies Made of an Engineered Spider Silk Protein
journal, December 2011

  • Leal-Egaña, Aldo; Lang, Gregor; Mauerer, Carolin
  • Advanced Engineering Materials, Vol. 14, Issue 3
  • DOI: 10.1002/adem.201180072

Nanolayer biomaterial coatings of silk fibroin for controlled release
journal, August 2007


Impact of initial solvent on thermal stability and mechanical properties of recombinant spider silk films
journal, January 2011

  • Spiess, Kristina; Ene, Roxana; Keenan, Caroline D.
  • Journal of Materials Chemistry, Vol. 21, Issue 35
  • DOI: 10.1039/c1jm11700a

Mussel adhesion is dictated by time-regulated secretion and molecular conformation of mussel adhesive proteins
journal, October 2015

  • Petrone, Luigi; Kumar, Akshita; Sutanto, Clarinda N.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms9737

Spider silk as archetypal protein elastomer
journal, January 2006

  • Vollrath, Fritz; Porter, David
  • Soft Matter, Vol. 2, Issue 5
  • DOI: 10.1039/b600098n

Robust Strategies for Automated AFM Force Curve Analysis—I. Non-adhesive Indentation of Soft, Inhomogeneous Materials
journal, November 2006

  • Lin, David C.; Dimitriadis, Emilios K.; Horkay, Ferenc
  • Journal of Biomechanical Engineering, Vol. 129, Issue 3
  • DOI: 10.1115/1.2720924

“Nano-Fishnet” Structure Making Silk Fibers Tougher
journal, June 2016

  • Liu, Ruchuan; Deng, Qinqiu; Yang, Zhen
  • Advanced Functional Materials, Vol. 26, Issue 30
  • DOI: 10.1002/adfm.201600813

Determining cantilever stiffness from thermal noise
journal, January 2013

  • Lübbe, Jannis; Temmen, Matthias; Rahe, Philipp
  • Beilstein Journal of Nanotechnology, Vol. 4
  • DOI: 10.3762/bjnano.4.23

Ion and seed dependent fibril assembly of a spidroin core domain
journal, August 2015

  • Humenik, Martin; Smith, Andrew M.; Arndt, Sina
  • Journal of Structural Biology, Vol. 191, Issue 2
  • DOI: 10.1016/j.jsb.2015.06.021

Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins
journal, October 2012


Direct Evidence for the Polymeric Nature of Polydopamine
journal, December 2018

  • Delparastan, Peyman; Malollari, Katerina G.; Lee, Haeshin
  • Angewandte Chemie International Edition, Vol. 58, Issue 4
  • DOI: 10.1002/anie.201811763

Substrate-Independent Layer-by-Layer Assembly by Using Mussel-Adhesive-Inspired Polymers
journal, May 2008


Estimating the Proportion of Healthcare-Associated Infections That Are Reasonably Preventable and the Related Mortality and Costs
journal, February 2011

  • Umscheid, Craig A.; Mitchell, Matthew D.; Doshi, Jalpa A.
  • Infection Control & Hospital Epidemiology, Vol. 32, Issue 2
  • DOI: 10.1086/657912

Protein adsorption kinetics in different surface potentials
journal, February 2008


Determination of Elastic Moduli of Thin Layers of Soft Material Using the Atomic Force Microscope
journal, May 2002


On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon
journal, January 2001

  • Nakanishi, Kazuhiro; Sakiyama, Takaharu; Imamura, Koreyoshi
  • Journal of Bioscience and Bioengineering, Vol. 91, Issue 3
  • DOI: 10.1016/S1389-1723(01)80127-4