Photo-controlled release of paclitaxel and model drugs from RNA pyramids

doi:10.1007/s12274-018-2174-x

Title: Photo-controlled release of paclitaxel and model drugs from RNA pyramids

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Abstract

Stimuli-responsive release of drugs from a nanocarrier in spatial-, temporal-, and dosage-controlled fashions is of great interest in the pharmaceutical industry. Paclitaxel is one of the most effective and popular chemotherapeutic drugs against a number of cancers such as metastatic or nonmetastatic breast cancer, non–small cell lung cancer, refractory ovarian cancer, AIDS-related Kaposi’s sarcoma, and head and neck cancers. Here, by taking the advantage of RNA nanotechnology in biomedical and material science, we developed a three-dimensional pyramid-shaped RNA nanocage for a photocontrolled release of cargo, using paclitaxel as a model drug. The light-triggered release of paclitaxel or fluorophore Cy5 was achieved by incorporation of photocleavable spacers into the RNA nanoparticles. Upon irradiation with ultraviolet light, cargos were rapidly released (within 5 min). In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed higher cytotoxicity as compared to RNA nanoparticles without the photocleavable spacer. The methodology provides proof of concept for the application of the light-triggered controlled release of drugs from RNA nanocages.

Authors:

Xu, Congcong ^[1]; Li, Hui ^[1]; Zhang, Kaiming ^[2]; Binzel, Daniel W. ^[1]; Yin, Hongran ^[1]; Chiu, Wah ^[3]; Guo, Peixuan ^[1]

The Ohio State Univ., Columbus, OH (United States). Center for RNA Nanobiotechnology and Nanomedicine; The Ohio State Univ., Columbus, OH (United States). Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy; The Ohio State Univ., Columbus, OH (United States). Dorothy M. Davis Heart and Lung Research Inst., College of Medicine and James Comprehensive Cancer Center
Stanford Univ., CA (United States). Dept. of Bioengineering, Microbiology and Immunology, and James H. Clark Center
Stanford Univ., CA (United States). Dept. of Bioengineering, Microbiology and Immunology, and James H. Clark Center; SLAC National Accelerator Lab., Menlo Park, CA (United States)

Publication Date:: 2018-09-17

Research Org.:: SLAC National Accelerator Lab., Menlo Park, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1490466

Grant/Contract Number:: AC02-76SF00515

Resource Type:: Accepted Manuscript

Journal Name:: Nano Research

Additional Journal Information:: Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1998-0124

Publisher:: Springer

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY; RNA nanotechnology; controlled release; paclitaxel; drug delivery; RNA nanoparticles; Phi29 three-way junction

Citation Formats


                    Xu, Congcong, Li, Hui, Zhang, Kaiming, Binzel, Daniel W., Yin, Hongran, Chiu, Wah, and Guo, Peixuan. Photo-controlled release of paclitaxel and model drugs from RNA pyramids.  United States: N. p., 2018. 
        Web.  doi:10.1007/s12274-018-2174-x.

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                    Xu, Congcong, Li, Hui, Zhang, Kaiming, Binzel, Daniel W., Yin, Hongran, Chiu, Wah, & Guo, Peixuan. Photo-controlled release of paclitaxel and model drugs from RNA pyramids.  United States.  doi:10.1007/s12274-018-2174-x.

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                    Xu, Congcong, Li, Hui, Zhang, Kaiming, Binzel, Daniel W., Yin, Hongran, Chiu, Wah, and Guo, Peixuan. Mon .  
        "Photo-controlled release of paclitaxel and model drugs from RNA pyramids".  United States.  doi:10.1007/s12274-018-2174-x.  https://www.osti.gov/servlets/purl/1490466.

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@article{osti_1490466,

  title        = {Photo-controlled release of paclitaxel and model drugs from RNA pyramids},

  author       = {Xu, Congcong and Li, Hui and Zhang, Kaiming and Binzel, Daniel W. and Yin, Hongran and Chiu, Wah and Guo, Peixuan},

  abstractNote = {Stimuli-responsive release of drugs from a nanocarrier in spatial-, temporal-, and dosage-controlled fashions is of great interest in the pharmaceutical industry. Paclitaxel is one of the most effective and popular chemotherapeutic drugs against a number of cancers such as metastatic or nonmetastatic breast cancer, non–small cell lung cancer, refractory ovarian cancer, AIDS-related Kaposi’s sarcoma, and head and neck cancers. Here, by taking the advantage of RNA nanotechnology in biomedical and material science, we developed a three-dimensional pyramid-shaped RNA nanocage for a photocontrolled release of cargo, using paclitaxel as a model drug. The light-triggered release of paclitaxel or fluorophore Cy5 was achieved by incorporation of photocleavable spacers into the RNA nanoparticles. Upon irradiation with ultraviolet light, cargos were rapidly released (within 5 min). In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed higher cytotoxicity as compared to RNA nanoparticles without the photocleavable spacer. The methodology provides proof of concept for the application of the light-triggered controlled release of drugs from RNA nanocages.},

  doi          = {10.1007/s12274-018-2174-x},

  journal      = {Nano Research},

  number       = 1,

  volume       = 12,

  place        = {United States},

  year         = {2018},

  month        = {9}

}

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DOI: 10.1007/s12274-018-2174-x

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