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Title: Characterization of Thylakoid Division Using Chloroplast Dividing Mutants in Arabidopsis

Abstract

Here, chloroplasts are double membrane bound organelles that are found in plants and algae. Their division requires a number of proteins to assemble into rings along the center of the organelle and to constrict in synchrony. Chloroplasts possess a third membrane system, the thylakoids, which house the majority of proteins responsible for the light-dependent reactions. The mechanism that allows chloroplasts to sort out and separate the intricate thylakoid membrane structures during organelle division remain unknown. By characterizing the sizes of thylakoids found in a number of different chloroplast division mutants in Arabidopsis, we show that thylakoids do not divide independently of the chloroplast division cycle. More specifically, we show that thylakoid division requires the formation of both the inner and the outer contractile rings of the chloroplast.

Authors:
 [1];  [1];  [1];  [1]
  1. Univ. of California, Davis, CA (United States)
Publication Date:
Research Org.:
Univ. of California, Davis, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
Contributing Org.:
University of California - Davis
OSTI Identifier:
1490426
Grant/Contract Number:  
FG02-03ER15405
Resource Type:
Accepted Manuscript
Journal Name:
BioRxiv
Additional Journal Information:
Journal Name: BioRxiv
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Ho, Jonathan, Kwan, Warren, Li, Vivian, and Theg, Steven M. Characterization of Thylakoid Division Using Chloroplast Dividing Mutants in Arabidopsis. United States: N. p., 2017. Web. doi:10.1101/200667.
Ho, Jonathan, Kwan, Warren, Li, Vivian, & Theg, Steven M. Characterization of Thylakoid Division Using Chloroplast Dividing Mutants in Arabidopsis. United States. doi:10.1101/200667.
Ho, Jonathan, Kwan, Warren, Li, Vivian, and Theg, Steven M. Tue . "Characterization of Thylakoid Division Using Chloroplast Dividing Mutants in Arabidopsis". United States. doi:10.1101/200667. https://www.osti.gov/servlets/purl/1490426.
@article{osti_1490426,
title = {Characterization of Thylakoid Division Using Chloroplast Dividing Mutants in Arabidopsis},
author = {Ho, Jonathan and Kwan, Warren and Li, Vivian and Theg, Steven M.},
abstractNote = {Here, chloroplasts are double membrane bound organelles that are found in plants and algae. Their division requires a number of proteins to assemble into rings along the center of the organelle and to constrict in synchrony. Chloroplasts possess a third membrane system, the thylakoids, which house the majority of proteins responsible for the light-dependent reactions. The mechanism that allows chloroplasts to sort out and separate the intricate thylakoid membrane structures during organelle division remain unknown. By characterizing the sizes of thylakoids found in a number of different chloroplast division mutants in Arabidopsis, we show that thylakoids do not divide independently of the chloroplast division cycle. More specifically, we show that thylakoid division requires the formation of both the inner and the outer contractile rings of the chloroplast.},
doi = {10.1101/200667},
journal = {BioRxiv},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {10}
}

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    Works referencing / citing this record:

    The Arabidopsis arc5 and arc6 mutations differentially affect plastid morphology in pavement and guard cells in the leaf epidermis
    journal, February 2018