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Title: Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone

Abstract

Yarrowia lipolytica, known to accumulate lipids intracellularly, lacks the cellulolytic enzymes needed to break down solid biomass directly. This study aimed to evaluate the potential metabolic burden of expressing core cellulolytic enzymes in an engineered high lipid-accumulating strain of Y. lipolytica. Three fungal cellulases, Talaromyces emersonii-Trichoderma reesei chimeric cellobiohydrolase I (chimeric-CBH I), T. reesei cellobiohydrolase II (CBH II), and T. reesei endoglucanase II (EG II) were expressed using three constitutive strong promoters as a single integrative expression block in a recently engineered lipid hyper-accumulating strain of Y. lipolytica (HA1). In yeast extract-peptone-dextrose (YPD) medium, the resulting cellulase co-expressing transformant YL165-1 had the chimeric-CBH I, CBH II, and EG II secretion titers being 26, 17, and 132 mg L -1, respectively. Cellulase co-expression in YL165-1 in culture media with a moderate C/N ratio of ~4.5 unexpectedly resulted in a nearly two-fold reduction in cellular lipid accumulation compared to the parental control strain, a sign of cellular metabolic drain. Such metabolic drain was ameliorated when grown in media with a high C/N ratio of 59 having a higher glucose utilization rate that led to approximately twofold more cell mass and threefold more lipid production per liter culture compared to parental control strain,more » suggesting cross-talk between cellulase and lipid production, both of which involve the endoplasmic reticulum (ER). Most importantly, we found that the chemical chaperone, trimethylamine N-oxide dihydride increased glucose utilization, cell mass and total lipid titer in the transformants, suggesting further amelioration of the metabolic drain. In conclusion, this is the first study examining lipid production in cellulase-expressing Y. lipolytica strains under various C/N ratio media and with a chemical chaperone highlighting the metabolic complexity for developing robust, cellulolytic and lipogenic yeast strains.« less

Authors:
ORCiD logo [1];  [1];  [2];  [1]; ORCiD logo [1];  [1];  [1];  [1]; ORCiD logo [1];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. The Univ. of Texas at Austin, Austin, TX (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1490107
Alternate Identifier(s):
OSTI ID: 1491724
Report Number(s):
NREL/JA-2700-73065
Journal ID: ISSN 1664-302X
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; fungal cellulolytic enzymes; Yarrowia lipolytica; cellulosic biofuel; cellobiohydrolase I; endoglucanase II; lipid metabolism; endoplasmic reticulum stress; chemical chaperone

Citation Formats

Wei, Hui, Wang, Wei, Alper, Hal S., Xu, Qi, Knoshaug, Eric P., Van Wychen, Stefanie, Lin, Chien -Yuan, Luo, Yonghua, Decker, Stephen R., Himmel, Michael E., and Zhang, Min. Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone. United States: N. p., 2019. Web. doi:10.3389/fmicb.2018.03276.
Wei, Hui, Wang, Wei, Alper, Hal S., Xu, Qi, Knoshaug, Eric P., Van Wychen, Stefanie, Lin, Chien -Yuan, Luo, Yonghua, Decker, Stephen R., Himmel, Michael E., & Zhang, Min. Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone. United States. doi:10.3389/fmicb.2018.03276.
Wei, Hui, Wang, Wei, Alper, Hal S., Xu, Qi, Knoshaug, Eric P., Van Wychen, Stefanie, Lin, Chien -Yuan, Luo, Yonghua, Decker, Stephen R., Himmel, Michael E., and Zhang, Min. Wed . "Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone". United States. doi:10.3389/fmicb.2018.03276.
@article{osti_1490107,
title = {Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone},
author = {Wei, Hui and Wang, Wei and Alper, Hal S. and Xu, Qi and Knoshaug, Eric P. and Van Wychen, Stefanie and Lin, Chien -Yuan and Luo, Yonghua and Decker, Stephen R. and Himmel, Michael E. and Zhang, Min},
abstractNote = {Yarrowia lipolytica, known to accumulate lipids intracellularly, lacks the cellulolytic enzymes needed to break down solid biomass directly. This study aimed to evaluate the potential metabolic burden of expressing core cellulolytic enzymes in an engineered high lipid-accumulating strain of Y. lipolytica. Three fungal cellulases, Talaromyces emersonii-Trichoderma reesei chimeric cellobiohydrolase I (chimeric-CBH I), T. reesei cellobiohydrolase II (CBH II), and T. reesei endoglucanase II (EG II) were expressed using three constitutive strong promoters as a single integrative expression block in a recently engineered lipid hyper-accumulating strain of Y. lipolytica (HA1). In yeast extract-peptone-dextrose (YPD) medium, the resulting cellulase co-expressing transformant YL165-1 had the chimeric-CBH I, CBH II, and EG II secretion titers being 26, 17, and 132 mg L-1, respectively. Cellulase co-expression in YL165-1 in culture media with a moderate C/N ratio of ~4.5 unexpectedly resulted in a nearly two-fold reduction in cellular lipid accumulation compared to the parental control strain, a sign of cellular metabolic drain. Such metabolic drain was ameliorated when grown in media with a high C/N ratio of 59 having a higher glucose utilization rate that led to approximately twofold more cell mass and threefold more lipid production per liter culture compared to parental control strain, suggesting cross-talk between cellulase and lipid production, both of which involve the endoplasmic reticulum (ER). Most importantly, we found that the chemical chaperone, trimethylamine N-oxide dihydride increased glucose utilization, cell mass and total lipid titer in the transformants, suggesting further amelioration of the metabolic drain. In conclusion, this is the first study examining lipid production in cellulase-expressing Y. lipolytica strains under various C/N ratio media and with a chemical chaperone highlighting the metabolic complexity for developing robust, cellulolytic and lipogenic yeast strains.},
doi = {10.3389/fmicb.2018.03276},
journal = {Frontiers in Microbiology},
number = ,
volume = 9,
place = {United States},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3389/fmicb.2018.03276

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Cited by: 1 work
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Figures / Tables:

FIGURE 1 FIGURE 1: | Pedigree of transformants expressing single or multiple cellulases and the experimental outline for transformant characterization. The details for these strains are described in Table 1. CBH, cellobiohydrolase; EG, endoglucanase; FAME, fatty acid methyl esters.

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Works referenced in this record:

Cloning and Expression of Trichoderma reesei Cellobiohydrolase I in Pichia pastoris
journal, October 1999

  • Godbole, S.; Decker, S. R.; Nieves, R. A.
  • Biotechnology Progress, Vol. 15, Issue 5
  • DOI: 10.1021/bp9901116

Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production
journal, January 2013

  • Chang, Jui-Jen; Ho, Feng-Ju; Ho, Cheng-Yu
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-19

Cloning and sequencing of the alkaline extracellular protease gene of Yarrowia lipolytica.
journal, October 1987


Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach
journal, May 2017

  • Guo, Zhong-peng; Duquesne, Sophie; Bozonnet, Sophie
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0819-8

Heterologous protein expression in Hypocrea jecorina: A historical perspective and new developments
journal, January 2015


Analyzing real-time PCR data by the comparative CT method
journal, June 2008


Stimulation of Lignocellulosic Biomass Hydrolysis by Proteins of Glycoside Hydrolase Family 61 Structure and Function of a Large, Enigmatic Family
journal, April 2010

  • Harris, Paul; Welner, Ditte; McFarland, K.
  • Biochemistry, Vol. 49, Issue 15, p. 3305-3316
  • DOI: 10.1021/bi100009p

Heterologous Expression of Xylanase Enzymes in Lipogenic Yeast Yarrowia lipolytica
journal, December 2014


Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi
journal, July 2017


Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates
journal, January 2013

  • Franden, Mary Ann; Pilath, Heidi M.; Mohagheghi, Ali
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-99

Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005

  • Lynd, Lee R.; van Zyl, Willem H.; McBride, John E.
  • Current Opinion in Biotechnology, Vol. 16, Issue 5, p. 577-583
  • DOI: 10.1016/j.copbio.2005.08.009

Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters
journal, January 2000


Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel
journal, January 2012


Muscle LIM protein/CSRP3: a mechanosensor with a role in autophagy
journal, August 2015


Production of oils and fats by oleaginous microorganisms with an emphasis given to the potential of the nonconventional yeast Yarrowia lipolytica
journal, April 2018


An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica
journal, May 2015


SGAtools: one-stop analysis and visualization of array-based genetic interaction screens
journal, May 2013

  • Wagih, Omar; Usaj, Matej; Baryshnikova, Anastasia
  • Nucleic Acids Research, Vol. 41, Issue W1
  • DOI: 10.1093/nar/gkt400

Overexpression of native Saccharomyces cerevisiae ER-to-Golgi SNARE genes increased heterologous cellulase secretion
journal, October 2015

  • Van Zyl, John Henry D.; Den Haan, Riaan; Van Zyl, Willem H.
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 1
  • DOI: 10.1007/s00253-015-7022-2

Magnesium Ions Improve Growth and Ethanol Production of Zymomonas mobilis under Heat or Ethanol Stress
journal, January 2007

  • ., Pornthap Thanonkeo; ., Pattana Laopaiboon; ., Kaewta Sootsuwan
  • Biotechnology(Faisalabad), Vol. 6, Issue 1
  • DOI: 10.3923/biotech.2007.112.119

Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose
journal, November 2016


Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars
journal, February 2008


Development and physiological characterization of cellobiose-consuming Yarrowia lipolytica : Development and Physiological Characterization
journal, January 2015

  • Lane, Stephan; Zhang, Shuyan; Wei, Na
  • Biotechnology and Bioengineering, Vol. 112, Issue 5
  • DOI: 10.1002/bit.25499

Efficient direct ethanol production from cellulose by cellulase- and cellodextrin transporter-co-expressing Saccharomyces cerevisiae
journal, January 2013


The metabolic burden of cellulase expression by recombinant Saccharomyces cerevisiae Y294 in aerobic batch culture
journal, April 2012

  • van Rensburg, Eugéne; den Haan, Riaan; Smith, Justin
  • Applied Microbiology and Biotechnology, Vol. 96, Issue 1
  • DOI: 10.1007/s00253-012-4037-9

Is fat so bad? Modulation of endoplasmic reticulum stress by lipid droplet formation
journal, June 2011

  • Hapala, Ivan; Marza, Esther; Ferreira, Thierry
  • Biology of the Cell, Vol. 103, Issue 6
  • DOI: 10.1042/BC20100144

Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica
journal, December 2015

  • Ryu, Seunghyun; Hipp, Julie; Trinh, Cong T.
  • Applied and Environmental Microbiology, Vol. 82, Issue 4
  • DOI: 10.1128/AEM.03582-15

Methods for measuring cellulase activities
journal, January 1988


Acceleration of ethanol production from paper mill waste fiber by supplementation with β-glucosidase
journal, January 1993


Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production
journal, August 2012

  • Fan, L. -H.; Zhang, Z. -J.; Yu, X. -Y.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 33
  • DOI: 10.1073/pnas.1209856109

Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga
journal, November 2012


Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans
journal, September 2008

  • Li, Yanqun; Horsman, Mark; Wang, Bei
  • Applied Microbiology and Biotechnology, Vol. 81, Issue 4
  • DOI: 10.1007/s00253-008-1681-1

Engineering xylose utilization in Yarrowia lipolytica by understanding its cryptic xylose pathway
journal, July 2016

  • Rodriguez, Gabriel M.; Hussain, Murtaza Shabbir; Gambill, Lauren
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0562-6

Monitoring Rhodosporidium toruloides NCYC 921 batch fermentations growing under carbon and nitrogen limitation by flow cytometry
journal, October 2011

  • Andrade, Raoni; Leal, Rodrigo; Roseiro, José
  • World Journal of Microbiology and Biotechnology, Vol. 28, Issue 3
  • DOI: 10.1007/s11274-011-0920-2

Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing
journal, October 2014


Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species
journal, September 2013


Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+ transformants
journal, July 2011


Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity
journal, January 2012

  • Wei, Hui; Tucker, Melvin P.; Baker, John O.
  • Biotechnology for Biofuels, Vol. 5, Issue 1
  • DOI: 10.1186/1754-6834-5-20

Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome
journal, March 2012

  • Sun, Jie; Wen, Fei; Si, Tong
  • Applied and Environmental Microbiology, Vol. 78, Issue 11
  • DOI: 10.1128/AEM.07679-11

Immobilization of Recombinant Invertase (re-INVB) from Zymomonas mobilis on d -Sorbitol Cinnamic Ester for Production of Invert Sugar
journal, February 2008

  • Vallejo-Becerra, Vanessa; Marín-Zamora, María Elisa; Vásquez-Bahena, Jazmin Magdalena
  • Journal of Agricultural and Food Chemistry, Vol. 56, Issue 4
  • DOI: 10.1021/jf072646h

Maximizing biodiesel production from Yarrowia lipolytica Po1g biomass using subcritical water pretreatment
journal, May 2012


Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
journal, January 2007


Enabling xylose utilization in Yarrowia lipolytica for lipid production
journal, July 2016


The Spatial Organization of Lipid Synthesis in the Yeast Saccharomyces cerevisiae Derived from Large Scale Green Fluorescent Protein Tagging and High Resolution Microscopy
journal, February 2005

  • Natter, Klaus; Leitner, Peter; Faschinger, Alexander
  • Molecular & Cellular Proteomics, Vol. 4, Issue 5
  • DOI: 10.1074/mcp.M400123-MCP200

Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis
journal, December 2009


Yeast recombination: the association between double-strand gap repair and crossing-over.
journal, July 1983

  • Orr-Weaver, T. L.; Szostak, J. W.
  • Proceedings of the National Academy of Sciences, Vol. 80, Issue 14
  • DOI: 10.1073/pnas.80.14.4417

A novel strain of Yarrowia lipolytica as a platform for value-added product synthesis from glycerol
journal, August 2016

  • Mirończuk, Aleksandra M.; Rzechonek, Dorota A.; Biegalska, Anna
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0593-z

A conserved family of proteins facilitates nascent lipid droplet budding from the ER
journal, October 2015

  • Choudhary, Vineet; Ojha, Namrata; Golden, Andy
  • The Journal of Cell Biology, Vol. 211, Issue 2
  • DOI: 10.1083/jcb.201505067

Simultaneous saccharification and fermentation of cellulose in ionic liquid for efficient production of α-ketoglutaric acid by Yarrowia lipolytica
journal, March 2015

  • Ryu, Seunghyun; Labbé, Nicole; Trinh, Cong T.
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 10
  • DOI: 10.1007/s00253-015-6521-5

Comparison of the Heterologous Expression of Trichoderma reesei Endoglucanase II and Cellobiohydrolase II in the Yeasts Pichia pastoris and Yarrowia lipolytica
journal, May 2012

  • Boonvitthya, Nassapat; Bozonnet, Sophie; Burapatana, Vorakan
  • Molecular Biotechnology, Vol. 54, Issue 2
  • DOI: 10.1007/s12033-012-9557-0

Genetic suppression of HO-1 exacerbates renal damage: reversed by an increase in the antiapoptotic signaling pathway
journal, January 2007

  • Olszanecki, Rafal; Rezzani, Rita; Omura, Shinji
  • American Journal of Physiology-Renal Physiology, Vol. 292, Issue 1
  • DOI: 10.1152/ajprenal.00261.2006

Optimization of enzyme complexes for lignocellulose hydrolysis
journal, January 2007

  • Berlin, Alex; Maximenko, Vera; Gilkes, Neil
  • Biotechnology and Bioengineering, Vol. 97, Issue 2, p. 287-296
  • DOI: 10.1002/bit.21238

Unraveling fatty acid transport and activation mechanisms in Yarrowia lipolytica
journal, September 2015

  • Dulermo, Rémi; Gamboa-Meléndez, Heber; Ledesma-Amaro, Rodrigo
  • Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1851, Issue 9
  • DOI: 10.1016/j.bbalip.2015.04.004

Cellobiohydrolase secretion by yeast: Current state and prospects for improvement
journal, January 2013


Connecting lignin-degradation pathway with pre-treatment inhibitor sensitivity of Cupriavidus necator
journal, May 2014


Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization
journal, May 2016

  • Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-016-0486-7

Sorbitol required for cell growth and ethanol production by Zymomonas mobilis under heat, ethanol, and osmotic stresses
journal, January 2013

  • Sootsuwan, Kaewta; Thanonkeo, Pornthap; Keeratirakha, Nawapote
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-180

Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids
journal, January 2016


High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production
journal, August 2016

  • Back, Alexandre; Rossignol, Tristan; Krier, François
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-016-0546-z

Effect of different C/N ratios on carotenoid and lipid production by Rhodotorula glutinis
journal, June 2013

  • Braunwald, Teresa; Schwemmlein, Lisa; Graeff-Hönninger, Simone
  • Applied Microbiology and Biotechnology, Vol. 97, Issue 14
  • DOI: 10.1007/s00253-013-5005-8

Structural and Biochemical Properties of Lipid Particles from the Yeast Saccharomyces cerevisiae
journal, April 2008

  • Czabany, Tibor; Wagner, Andrea; Zweytick, Dagmar
  • Journal of Biological Chemistry, Vol. 283, Issue 25
  • DOI: 10.1074/jbc.M800401200

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica
journal, March 2017

  • Mirończuk, Aleksandra M.; Biegalska, Anna; Dobrowolski, Adam
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0772-6

Reduction of trimethylamine N-oxide byEscherichia coli as anaerobic respiration
journal, January 1978

  • Ishimoto, M.; Shimokawa, O.
  • Zeitschrift für allgemeine Mikrobiologie, Vol. 18, Issue 3
  • DOI: 10.1002/jobm.3630180304

Lipid droplets are functionally connected to the endoplasmic reticulum in Saccharomyces cerevisiae
journal, June 2011

  • Jacquier, N.; Choudhary, V.; Mari, M.
  • Journal of Cell Science, Vol. 124, Issue 14
  • DOI: 10.1242/jcs.076836

Trends and novel strategies for enhancing lipid accumulation and quality in microalgae
journal, March 2016

  • Singh, Poonam; Kumari, Sheena; Guldhe, Abhishek
  • Renewable and Sustainable Energy Reviews, Vol. 55
  • DOI: 10.1016/j.rser.2015.11.001

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae
journal, January 2011

  • Ilmén, Marja; den Haan, Riaan; Brevnova, Elena
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-30

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Effects of wet ball-milling on particle dispersion and mechanical properties of particulate epoxy composites
journal, February 1996


Efficient conversion of crude glycerol from various industrial wastes into single cell oil by yeast Yarrowia lipolytica
journal, May 2016


Trimethylamine oxide reduction by Salmonella
journal, December 1974

  • Kim, Kyung Eun; Chang, George W.
  • Canadian Journal of Microbiology, Vol. 20, Issue 12
  • DOI: 10.1139/m74-269

Comparison of Nitrogen Depletion and Repletion on Lipid Production in Yeast and Fungal Species
journal, August 2016


Genome-wide analysis of the endoplasmic reticulum stress response during lignocellulase production in Neurospora crassa
journal, April 2015


Secretory Protein Biogenesis and Traffic in the Early Secretory Pathway
journal, February 2013


Enzymatic properties of cellulases from Humicola insolens
journal, September 1997


High Lipid Induction in Microalgae for Biodiesel Production
journal, May 2012

  • Sharma, Kalpesh K.; Schuhmann, Holger; Schenk, Peer M.
  • Energies, Vol. 5, Issue 5
  • DOI: 10.3390/en5051532

Protocols and Programs for High-Throughput Growth and Aging Phenotyping in Yeast
journal, March 2015


Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production
journal, January 2013


Expression of oleosin and perilipins in yeast promotes formation of lipid droplets from the endoplasmic reticulum
journal, September 2013

  • Jacquier, N.; Mishra, S.; Choudhary, V.
  • Journal of Cell Science, Vol. 126, Issue 22
  • DOI: 10.1242/jcs.131896

Recent progress in consolidated bioprocessing
journal, June 2012

  • Olson, Daniel G.; McBride, John E.; Joe Shaw, A.
  • Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405
  • DOI: 10.1016/j.copbio.2011.11.026

Chemicals to enhance microalgal growth and accumulation of high-value bioproducts
journal, February 2015


Understanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach
journal, January 2018

  • de Ruijter, Jorg C.; Koskela, Essi V.; Nandania, Jatin
  • Yeast, Vol. 35, Issue 4
  • DOI: 10.1002/yea.3298

Correlating the ability of lignocellulosic polymers to constrain water with the potential to inhibit cellulose saccharification
journal, November 2014

  • Selig, Michael J.; Thygesen, Lisbeth G.; Felby, Claus
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/s13068-014-0159-x

Metabolic engineering of Yarrowia lipolytica for industrial applications
journal, December 2015


Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid
journal, January 2013


The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources
journal, July 2016

  • Gientka, Iwona; Bzducha-Wróbel, Anna; Stasiak-Różańska, Lidia
  • Electronic Journal of Biotechnology, Vol. 22
  • DOI: 10.1016/j.ejbt.2016.02.008

Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective
journal, April 2014


Design and characterisation of synthetic operons for biohydrogen technology
journal, November 2016


Influence of ylHog1 MAPK kinase on Yarrowia lipolytica stress response and erythritol production
journal, October 2018


Metabolic engineering in the host Yarrowia lipolytica
journal, November 2018


Effects of varying media, temperature, and growth rates on the intracellular concentrations of yeast amino acids
journal, July 1995

  • Martinez-Force, Enrique; Benitez, Tahia
  • Biotechnology Progress, Vol. 11, Issue 4
  • DOI: 10.1021/bp00034a004

Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes
journal, August 2015

  • Guo, Zhongpeng; Duquesne, Sophie; Bozonnet, Sophie
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0289-9

Mixtures of Thermostable Enzymes Show High Performance in Biomass Saccharification
journal, April 2014

  • Kallioinen, Anne; Puranen, Terhi; Siika-aho, Matti
  • Applied Biochemistry and Biotechnology, Vol. 173, Issue 5
  • DOI: 10.1007/s12010-014-0893-3

Yarrowia lipolytica and Its Multiple Applications in the Biotechnological Industry
journal, January 2014

  • Gonçalves, F. A. G.; Colen, G.; Takahashi, J. A.
  • The Scientific World Journal, Vol. 2014
  • DOI: 10.1155/2014/476207

Conditions of endoplasmic reticulum stress stimulate lipid droplet formation in Saccharomyces cerevisiae
journal, October 2009

  • Fei, Weihua; Wang, Han; Fu, Xin
  • Biochemical Journal, Vol. 424, Issue 1
  • DOI: 10.1042/BJ20090785

Quantitative analysis of fitness and genetic interactions in yeast on a genome scale
journal, November 2010

  • Baryshnikova, Anastasia; Costanzo, Michael; Kim, Yungil
  • Nature Methods, Vol. 7, Issue 12
  • DOI: 10.1038/nmeth.1534

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production
journal, January 2014

  • Blazeck, John; Hill, Andrew; Liu, Leqian
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4131

Oleaginous yeasts for biodiesel: Current and future trends in biology and production
journal, November 2014