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Title: Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 Administration Reduces the Numbers of Candida albicans and Abundance of Enterobacteria in the Gut Microbiota of Familial Mediterranean Fever Patients

Abstract

Intestinal microorganisms play a crucial role in health and disease. The disruption of host–microbiota homeostasis has been reported to occur not only during disease development but also as a result of medication. Familial Mediterranean fever (FMF) is an inflammatory genetic disease characterized by elevated systemic reactivity against the commensal gut microbiota and high levels of Candida albicans in the gut. This study’s major objective was to investigate the effects of commercial probiotic Narine on the relative abundance of gut bacteria (specifically, enterobacteria, lactobacilli, Staphylococcus aureus, and enterococci) of C. albicans carrier and non-carrier FMF patients in remission. Our main finding indicates that the probiotic reduces numbers of C. albicans and abundance of enterobacteria in male and female patients of C. albicans carriers and non-carriers. It has pivotal effect on Enterococcus faecalis: increase in male non-carriers and decrease in female ones regardless of C. albicans status. No effect was seen for Lactobacillus and S. aureus. Our data suggest that M694V/V726A pyrin inflammasome mutations leading to FMF disease may contribute to gender-specific differences in microbial community structure in FMF patients. The study’s secondary objective was to elucidate the gender-specific differences in the gut’s microbial community of FMF patients. The tendency was detectedmore » for higher counts of enterobacteria in female FMF subjects. However, the small number of patients of these groups preclude from conclusive statements, pointing at the need for additional investigations with appropriate for statistical analysis groups of subjects involved in the study.« less

Authors:
 [1];  [2];  [1];  [3];  [2];  [4];  [3];  [5];  [6];  [7]
  1. Armenian National Agrarian Univ., Yerevan (Armenia). Dept. of Food Safety and Biotechnology
  2. International Association for Human and Animals Health Improvement, Yerevan (Armenia)
  3. Yerevan State Medical Univ., Yerevan (Armenia)
  4. Armenian National Agrarian Univ., Yerevan (Armenia)
  5. Kyorin Univ., Tokyo (Japan). School of Medicine
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  7. Rutgers Univ., New Brunswick, NJ (United States). Health Promoting Naturals Lab. School of Environmental and Biological Sciences
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA), Office of Defense Nuclear Nonproliferation; International Science and Technology Center (ISTC)
OSTI Identifier:
1489661
Grant/Contract Number:  
AC02-05CH11231; A-1980; A-2134
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Immunology
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-3224
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; familial Mediterranean fever; M694V/V726A mutations; gut microbiota; gender; Candida albicans; Lactobacillus acidophilus INMIA 9602 Er 317/402; Enteroccocus faecalis; Enterobacteriaceae

Citation Formats

Pepoyan, Astghik, Balayan, Marine, Manvelyan, Anahit, Galstyan, Lilit, Pepoyan, Sofi, Petrosyan, Susanna, Tsaturyan, Vardan, Kamiya, Shigeru, Torok, Tamas, and Chikindas, Michael. Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 Administration Reduces the Numbers of Candida albicans and Abundance of Enterobacteria in the Gut Microbiota of Familial Mediterranean Fever Patients. United States: N. p., 2018. Web. doi:10.3389/fimmu.2018.01426.
Pepoyan, Astghik, Balayan, Marine, Manvelyan, Anahit, Galstyan, Lilit, Pepoyan, Sofi, Petrosyan, Susanna, Tsaturyan, Vardan, Kamiya, Shigeru, Torok, Tamas, & Chikindas, Michael. Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 Administration Reduces the Numbers of Candida albicans and Abundance of Enterobacteria in the Gut Microbiota of Familial Mediterranean Fever Patients. United States. doi:10.3389/fimmu.2018.01426.
Pepoyan, Astghik, Balayan, Marine, Manvelyan, Anahit, Galstyan, Lilit, Pepoyan, Sofi, Petrosyan, Susanna, Tsaturyan, Vardan, Kamiya, Shigeru, Torok, Tamas, and Chikindas, Michael. Tue . "Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 Administration Reduces the Numbers of Candida albicans and Abundance of Enterobacteria in the Gut Microbiota of Familial Mediterranean Fever Patients". United States. doi:10.3389/fimmu.2018.01426. https://www.osti.gov/servlets/purl/1489661.
@article{osti_1489661,
title = {Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 Administration Reduces the Numbers of Candida albicans and Abundance of Enterobacteria in the Gut Microbiota of Familial Mediterranean Fever Patients},
author = {Pepoyan, Astghik and Balayan, Marine and Manvelyan, Anahit and Galstyan, Lilit and Pepoyan, Sofi and Petrosyan, Susanna and Tsaturyan, Vardan and Kamiya, Shigeru and Torok, Tamas and Chikindas, Michael},
abstractNote = {Intestinal microorganisms play a crucial role in health and disease. The disruption of host–microbiota homeostasis has been reported to occur not only during disease development but also as a result of medication. Familial Mediterranean fever (FMF) is an inflammatory genetic disease characterized by elevated systemic reactivity against the commensal gut microbiota and high levels of Candida albicans in the gut. This study’s major objective was to investigate the effects of commercial probiotic Narine on the relative abundance of gut bacteria (specifically, enterobacteria, lactobacilli, Staphylococcus aureus, and enterococci) of C. albicans carrier and non-carrier FMF patients in remission. Our main finding indicates that the probiotic reduces numbers of C. albicans and abundance of enterobacteria in male and female patients of C. albicans carriers and non-carriers. It has pivotal effect on Enterococcus faecalis: increase in male non-carriers and decrease in female ones regardless of C. albicans status. No effect was seen for Lactobacillus and S. aureus. Our data suggest that M694V/V726A pyrin inflammasome mutations leading to FMF disease may contribute to gender-specific differences in microbial community structure in FMF patients. The study’s secondary objective was to elucidate the gender-specific differences in the gut’s microbial community of FMF patients. The tendency was detected for higher counts of enterobacteria in female FMF subjects. However, the small number of patients of these groups preclude from conclusive statements, pointing at the need for additional investigations with appropriate for statistical analysis groups of subjects involved in the study.},
doi = {10.3389/fimmu.2018.01426},
journal = {Frontiers in Immunology},
number = ,
volume = 9,
place = {United States},
year = {2018},
month = {6}
}

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Cited by: 3 works
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Figures / Tables:

Table 1 Table 1: Hybidization scores for gut bacteria in Candida albicans-carrier and non-carrier familial Mediterranean fever (FMF) patients after probioticaa therapy; average ± SD.

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      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.