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Title: Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Here, we developed a CRISPR/Cas9 and homology-directed-repair assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ;  [2] ;  [1] ;  [1] ;  [3] ; ORCiD logo [4]
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  2. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Shanghai Jiao Tong Univ., Shanghai (China)
  3. Metabolic Engineering Research Lab. (Singapore)
  4. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Metabolic Engineering Research Lab. (Singapore)
Publication Date:
Grant/Contract Number:
SC0018260
Type:
Accepted Manuscript
Journal Name:
Nature Biotechnology
Additional Journal Information:
Journal Volume: 36; Journal Issue: 6; Journal ID: ISSN 1087-0156
Publisher:
Springer Nature
Research Org:
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1489638

Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, and Zhao, Huimin. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. United States: N. p., Web. doi:10.1038/nbt.4132.
Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, & Zhao, Huimin. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. United States. doi:10.1038/nbt.4132.
Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, and Zhao, Huimin. 2018. "Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision". United States. doi:10.1038/nbt.4132.
@article{osti_1489638,
title = {Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision},
author = {Bao, Zehua and HamediRad, Mohammad and Xue, Pu and Xiao, Han and Tasan, Ipek and Chao, Ran and Liang, Jing and Zhao, Huimin},
abstractNote = {Here, we developed a CRISPR/Cas9 and homology-directed-repair assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.},
doi = {10.1038/nbt.4132},
journal = {Nature Biotechnology},
number = 6,
volume = 36,
place = {United States},
year = {2018},
month = {5}
}

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