Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway

Cai, Guodong; Si, Mengxue; Li, Xi; Zou, Hui; Gu, Jianhong; Yuan, Yan; Liu, Xuezhong; Liu, Zongping; Bian, Jianchun

doi:10.1002/tox.22696

Title: Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway

Abstract

Abstract Zearalenone (ZEA) is an estrogen‐like toxin produced by Fusarium that is widely found in cereals worldwide. In recent years, ZEA has been found to cause reproductive dysfunction in male animals, but the underlying mechanism remains unclear. This study examined the apoptosis of rat Sertoli cells induced by different concentrations (0, 5, 10, and 20 μmol/L) of ZEA via Fas‐Fas ligand and mitochondrial signaling pathway in vitro. Apoptosis rate was detected by flow cytometry. The mitochondrial membrane potential was detected by immunofluorescence assay and flow cytometry. Western Blot and qRT‐PCR were used to identify the signaling pathway. The results revealed that ZEA induced apoptosis of rat Sertoli cells, significantly reduced the transcription and expression of the anti‐apoptotic protein Bcl‐2, increased the transcription and expression of pro‐apoptotic proteins Bax and tBID, and Fas, FasL, FADD, and caspase‐8. ZEA also increased the activation of caspase‐8 and caspase‐9, and promoted the release of cytochrome C from mitochondria to cytoplasm. Moreover, addition of caspase‐8 inhibitor Z‐IETD‐FMK led to significant decrease in the mitochondrial membrane potential and apoptosis rate of the ZEA + Z‐IETD‐FMK group as compared to the ZEA treatment group. The release of cytochrome C from mitochondria to cytoplasm and the activation of caspase‐9 and caspase‐3more »« less

Authors:

Cai, Guodong ^[1]; Si, Mengxue ^[2]; Li, Xi ^[2]; Zou, Hui ^[2]; Gu, Jianhong ^[2]; Yuan, Yan ^[2]; Liu, Xuezhong ^[2]; Liu, Zongping ^[1];

^[1]

College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China, Jiangsu Co‐innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou Jiangsu China, Joint International Research Laboratory of Agriculture and Agri‐Product Safety of the Ministry of Education of China Yangzhou University Yangzhou Jiangsu China
College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China, Jiangsu Co‐innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou Jiangsu China

Publication Date:: Tue Jan 01 00:00:00 EST 2019

Sponsoring Org.:: USDOE

OSTI Identifier:: 1489294

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Environmental Toxicology

Additional Journal Information:: Journal Name: Environmental Toxicology Journal Volume: 34 Journal Issue: 4; Journal ID: ISSN 1520-4081

Publisher:: Wiley Blackwell (John Wiley & Sons)

Country of Publication:: United States

Language:: English

Citation Formats


                    Cai, Guodong, Si, Mengxue, Li, Xi, Zou, Hui, Gu, Jianhong, Yuan, Yan, Liu, Xuezhong, Liu, Zongping, and Bian, Jianchun. Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway.  United States: N. p., 2019. 
Web.  doi:10.1002/tox.22696.

Copy to clipboard


                    Cai, Guodong, Si, Mengxue, Li, Xi, Zou, Hui, Gu, Jianhong, Yuan, Yan, Liu, Xuezhong, Liu, Zongping, & Bian, Jianchun. Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway.  United States.  https://doi.org/10.1002/tox.22696

Copy to clipboard


                    Cai, Guodong, Si, Mengxue, Li, Xi, Zou, Hui, Gu, Jianhong, Yuan, Yan, Liu, Xuezhong, Liu, Zongping, and Bian, Jianchun. Tue .  
"Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway".  United States.  https://doi.org/10.1002/tox.22696.

Copy to clipboard


                    
@article{osti_1489294,

  title        = {Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway},

  author       = {Cai, Guodong and Si, Mengxue and Li, Xi and Zou, Hui and Gu, Jianhong and Yuan, Yan and Liu, Xuezhong and Liu, Zongping and Bian, Jianchun},

  abstractNote = {Abstract Zearalenone (ZEA) is an estrogen‐like toxin produced by Fusarium that is widely found in cereals worldwide. In recent years, ZEA has been found to cause reproductive dysfunction in male animals, but the underlying mechanism remains unclear. This study examined the apoptosis of rat Sertoli cells induced by different concentrations (0, 5, 10, and 20 μmol/L) of ZEA via Fas‐Fas ligand and mitochondrial signaling pathway in vitro. Apoptosis rate was detected by flow cytometry. The mitochondrial membrane potential was detected by immunofluorescence assay and flow cytometry. Western Blot and qRT‐PCR were used to identify the signaling pathway. The results revealed that ZEA induced apoptosis of rat Sertoli cells, significantly reduced the transcription and expression of the anti‐apoptotic protein Bcl‐2, increased the transcription and expression of pro‐apoptotic proteins Bax and tBID, and Fas, FasL, FADD, and caspase‐8. ZEA also increased the activation of caspase‐8 and caspase‐9, and promoted the release of cytochrome C from mitochondria to cytoplasm. Moreover, addition of caspase‐8 inhibitor Z‐IETD‐FMK led to significant decrease in the mitochondrial membrane potential and apoptosis rate of the ZEA + Z‐IETD‐FMK group as compared to the ZEA treatment group. The release of cytochrome C from mitochondria to cytoplasm and the activation of caspase‐9 and caspase‐3 were significantly decreased in the ZEA + Z‐IETD‐FMK group. These results suggested that ZEA can induce apoptosis of rat Sertoli cells, activate the Fas‐Fas ligand signaling pathway and participate in the regulation of mitochondrial apoptosis pathway.},

  doi          = {10.1002/tox.22696},

  journal      = {Environmental Toxicology},

  number       = 4,

  volume       = 34,

  place        = {United States},

  year         = {Tue Jan 01 00:00:00 EST 2019},

  month        = {Tue Jan 01 00:00:00 EST 2019}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Publisher's Version of Record
https://doi.org/10.1002/tox.22696

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 31 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Involvement of cyclin D1 and E2f1 in zearalenone-induced DNA damage in testis of rats
journal, November 2015

Cheraghi, Shabnam; Razi, Mazdak; Malekinejad, Hassan
Toxicon, Vol. 106
DOI: 10.1016/j.toxicon.2015.09.018

Crocin protects human embryonic kidney cells (HEK293) from α- and β-Zearalenol-induced ER stress and apoptosis
journal, April 2016

Ben Salem, Intidhar; Boussabbeh, Manel; Prola, Alexandre
Environmental Science and Pollution Research, Vol. 23, Issue 15
DOI: 10.1007/s11356-016-6741-y

Death Receptors: Signaling and Modulation
journal, August 1998

Ashkenazi, A.
Science, Vol. 281, Issue 5381
DOI: 10.1126/science.281.5381.1305

Involvement of the Fas and Fas ligand in testicular germ cell apoptosis by zearalenone in rat
journal, January 2010

Jee, Youngheun; Noh, Eun-Mi; Cho, Eun-Sang
Journal of Veterinary Science, Vol. 11, Issue 2
DOI: 10.4142/jvs.2010.11.2.115

A Novel Protein That Interacts with the Death Domain of Fas/APO1 Contains a Sequence Motif Related to the Death Domain
journal, April 1995

Boldin, Mark P.; Varfolomeev, Eugene E.; Pancer, Zeev
Journal of Biological Chemistry, Vol. 270, Issue 14
DOI: 10.1074/jbc.270.14.7795

Participation of the Fas-Signaling System in the Initiation of Germ Cell Apoptosis in Young Rat Testes after Exposure to Mono-(2-Ethylhexyl) Phthalate
journal, November 1999

Richburg, John H.; Nañez, Adrian; Gao, Hong
Toxicology and Applied Pharmacology, Vol. 160, Issue 3
DOI: 10.1006/taap.1999.8786

Influence of in vitro exposure to mycotoxin zearalenone and its derivatives on swine sperm quality
journal, August 2008

Benzoni, E.; Minervini, F.; Giannoccaro, A.
Reproductive Toxicology, Vol. 25, Issue 4
DOI: 10.1016/j.reprotox.2008.04.009

Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease
journal, December 2005

Fadeel, B.; Orrenius, S.
Journal of Internal Medicine, Vol. 258, Issue 6
DOI: 10.1111/j.1365-2796.2005.01570.x

The TNF and TNF Receptor Superfamilies
journal, February 2001

Locksley, Richard M.; Killeen, Nigel; Lenardo, Michael J.
Cell, Vol. 104, Issue 4
DOI: 10.1016/S0092-8674(01)00237-9

Zearalenone inhibits testosterone biosynthesis in mouse Leydig cells via the crosstalk of estrogen receptor signaling and orphan nuclear receptor Nur77 expression
journal, June 2014

Liu, Qing; Wang, Yajun; Gu, Jianhong
Toxicology in Vitro, Vol. 28, Issue 4
DOI: 10.1016/j.tiv.2014.01.013

Exploration of Bcl-2 family and caspases-dependent apoptotic signaling pathway in Zearalenone-treated mouse endometrial stromal cells
journal, August 2016

Hu, Jin; Xu, Minglong; Dai, Yujian
Biochemical and Biophysical Research Communications, Vol. 476, Issue 4
DOI: 10.1016/j.bbrc.2016.05.161

The mycotoxin Zearalenone induces apoptosis in human hepatocytes (HepG2) via p53-dependent mitochondrial signaling pathway
journal, October 2008

Ayed-Boussema, Imen; Bouaziz, Chayma; Rjiba, Karima
Toxicology in Vitro, Vol. 22, Issue 7
DOI: 10.1016/j.tiv.2008.06.016

The biochemistry of apoptosis
journal, October 2000

Hengartner, Michael O.
Nature, Vol. 407, Issue 6805
DOI: 10.1038/35037710

Deletion of the BH1 Domain of Bcl-2 Accelerates Apoptosis by Acting in a Dominant Negative Fashion
journal, March 2003

Kawatani, Makoto; Imoto, Masaya
Journal of Biological Chemistry, Vol. 278, Issue 22
DOI: 10.1074/jbc.M213038200

Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway
journal, January 2012

Zhu, Li; Yuan, Hui; Guo, Chengzhi
Journal of Cellular Physiology, Vol. 227, Issue 5
DOI: 10.1002/jcp.22906

Occurrence, detection and detoxification of mycotoxins
journal, November 2015

Aiko, Visenuo; Mehta, Alka
Journal of Biosciences, Vol. 40, Issue 5
DOI: 10.1007/s12038-015-9569-6

Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells
journal, March 2016

Zheng, WangLong; Pan, ShunYe; Wang, Guangguang
Environmental Toxicology and Pharmacology, Vol. 42
DOI: 10.1016/j.etap.2016.01.013

Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells
journal, December 2010

Banjerdpongchai, Ratana; Kongtawelert, Prachya; Khantamat, Orawan
Journal of Hematology & Oncology, Vol. 3, Issue 1
DOI: 10.1186/1756-8722-3-50

Effects of PFNA exposure on expression of junction-associated molecules and secretory function in rat Sertoli cells
journal, November 2010

Feng, Yixing; Fang, Xuemei; Shi, Zhimin
Reproductive Toxicology, Vol. 30, Issue 3
DOI: 10.1016/j.reprotox.2010.05.010

Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis
journal, March 2009

Zhang, Zhen; Shao, Shan; Shetty, Gunapala
REPRODUCTION, Vol. 137, Issue 3
DOI: 10.1530/REP-08-0120

Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis
journal, August 1998

Li, Honglin; Zhu, Hong; Xu, Chi-jie
Cell, Vol. 94, Issue 4
DOI: 10.1016/S0092-8674(00)81590-1

Regulation of death receptor-induced apoptosis induced via CD95/Fas and other death receptors
journal, February 2011

Lavrik, I. N.
Molecular Biology, Vol. 45, Issue 1
DOI: 10.1134/S0026893311010079

The influence of the mycotoxins deoxynivalenol and zearalenol on in vitro maturation of pig oocytes and in vitro culture of pig zygotes
journal, December 2002

Alm, H.; Greising, T.; Brüssow, K. -P
Toxicology in Vitro, Vol. 16, Issue 6
DOI: 10.1016/S0887-2333(02)00059-0

Involvement of the Fas/FasL pathway in the pathogenesis of germ cell tumours of the adult testis
journal, January 2002

Kersemaekers, Anne-Marie F.; van Weeren, Pascale C.; Wolter Oosterhuis, J.
The Journal of Pathology, Vol. 196, Issue 4
DOI: 10.1002/path.1058

Involvement of MACH, a Novel MORT1/FADD-Interacting Protease, in Fas/APO-1- and TNF Receptor–Induced Cell Death
journal, June 1996

Boldin, Mark P.; Goncharov, Tanya M.; Goltseve, Yury V.
Cell, Vol. 85, Issue 6
DOI: 10.1016/S0092-8674(00)81265-9

Effect of lead on Sertoli–germ cell coculture of rat
journal, July 2000

Adhikari, Neeta; Sinha, Neelima; Saxena, D. K.
Toxicology Letters, Vol. 116, Issue 1-2
DOI: 10.1016/S0378-4274(00)00199-5

Programmed Cell Death in Animal Development
journal, February 1997

Jacobson, Michael D.; Weil, Miguel; Raff, Martin C.
Cell, Vol. 88, Issue 3
DOI: 10.1016/S0092-8674(00)81873-5

Prognostic Significance of Soluble fas in the Serum of Patients with Bladder Cancer
journal, August 1998

Mizutani, Youichi; Yoshida, Osamu; Bonavida, Benjamin
Journal of Urology, Vol. 160, Issue 2
DOI: 10.1016/S0022-5347(01)62960-4

FADD, a novel death domain-containing protein, interacts with the death domain of fas and initiates apoptosis
journal, May 1995

Chinnaiyan, Arul M.; O'Rourke, Karen; Tewari, Muneesh
Cell, Vol. 81, Issue 4
DOI: 10.1016/0092-8674(95)90071-3

The influence of N-acetyl-l-cysteine on damage of porcine oocyte exposed to zearalenone in vitro
journal, December 2015

Lai, Fang-Nong; Ma, Jun-Yu; Liu, Jing-Cai
Toxicology and Applied Pharmacology, Vol. 289, Issue 2
DOI: 10.1016/j.taap.2015.09.010

Dose-response effects of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm
journal, January 2011

Filannino, Angela; Stout, Tom AE; Gadella, Bart M.
Reproductive Biology and Endocrinology, Vol. 9, Issue 1
DOI: 10.1186/1477-7827-9-134

Similar Records in DOE PAGES and OSTI.GOV collections:

Mollugin induces apoptosis in human Jurkat T cells through endoplasmic reticulum stress-mediated activation of JNK and caspase-12 and subsequent activation of mitochondria-dependent caspase cascade regulated by Bcl-xL

Journal Article Kim, Sun Mi ; Park, Hae Sun ; Jun, Do Youn ; ... - Toxicology and Applied Pharmacology

Exposure of Jurkat T cells to mollugin (15-30 muM), purified from the roots of Rubia cordifolia L., caused cytotoxicity and apoptotic DNA fragmentation along with mitochondrial membrane potential disruption, mitochondrial cytochrome c release, phosphorylation of c-Jun N-terminal kinase (JNK), activation of caspase-12, -9, -7, -3, and -8, cleavage of FLIP and Bid, and PARP degradation, without accompanying necrosis. While these mollugin-induced cytotoxicity and apoptotic events including activation of caspase-8 and mitochondria-dependent activation of caspase cascade were completely prevented by overexpression of Bcl-xL, the activation of JNK and caspase-12 was prevented to much lesser extent. Pretreatment of the cells with themore »« less
https://doi.org/10.1016/j.taap.2009.08.024
Cytotoxicity of diacetoxyscirpenol is associated with apoptosis by activation of caspase-8 and interruption of cell cycle progression by down-regulation of cdk4 and cyclin B1 in human Jurkat T cells

Journal Article Jun, Do Youn ; Institute of Genetic Engineering, Kyungpook National University, Daegu ; Kim, Jun Seok ; ... - Toxicology and Applied Pharmacology

To understand the mechanism underlying T-cell toxicity of diacetoxyscirpenol (DAS) from Fusarium sambucinum, its apoptogenic as well as growth retardation activity was investigated in human Jurkat T cells. Exposure to DAS (0.01-0.15 {mu}M) caused apoptotic DNA fragmentation along with caspase-8 activation, Bid cleavage, mitochondrial cytochrome c release, activation of caspase-9 and caspase-3, and PARP degradation, without any alteration in the levels of Fas or FasL. Under these conditions, necrosis was not accompanied. The cytotoxicity of DAS was not blocked by the anti-Fas neutralizing antibody ZB-4. Although the DAS-induced apoptotic events were completely prevented by overexpression of Bcl-xL, the cells overexpressingmore »« less
https://doi.org/10.1016/j.taap.2007.04.011
17{alpha}-Estradiol arrests cell cycle progression at G{sub 2}/M and induces apoptotic cell death in human acute leukemia Jurkat T cells

Journal Article Jun, Do Youn ; Park, Hae Sun ; Kim, Jun Seok ; ... - Toxicology and Applied Pharmacology

A pharmacological dose (2.5-10 {mu}M) of 17{alpha}-estradiol (17{alpha}-E{sub 2}) exerted a cytotoxic effect on human leukemias Jurkat T and U937 cells, which was not suppressed by the estrogen receptor (ER) antagonist ICI 182,780. Along with cytotoxicity in Jurkat T cells, several apoptotic events including mitochondrial cytochrome c release, activation of caspase-9, -3, and -8, PARP degradation, and DNA fragmentation were induced. The cytotoxicity of 17{alpha}-E{sub 2} was not blocked by the anti-Fas neutralizing antibody ZB-4. While undergoing apoptosis, there was a remarkable accumulation of G{sub 2}/M cells with the upregulatoin of cdc2 kinase activity, which was reflected in the Thr56more »« less
https://doi.org/10.1016/j.taap.2008.05.023
CD437, a synthetic retinoid, induces apoptosis in human respiratory epithelial cells via caspase-independent mitochondrial and caspase-8-dependent pathways both up-regulated by JNK signaling pathway

Journal Article Boisvieux-Ulrich, Emmanuelle ; Sourdeval, Matthieu ; Marano, Francelyne - Experimental Cell Research

The synthetic retinoid-related molecule CD437-induced apoptosis in human epithelial airway respiratory cells: the 16HBE bronchial cell line and normal nasal epithelial cells. CD437 caused apoptosis in S-phase cells and cell cycle arrest in S phase. Apoptosis was abolished by caspase-8 inhibitor z-IETD-fmk which preserved S-phase cells but was weakly inhibited by others selective caspase-inhibitors, indicating that caspase-8 activation was involved. z-VAD and z-IETD prevented the nuclear envelope fragmentation but did not block the chromatin condensation. The disruption of mitochondrial transmembrane potential was also induced by CD437 treatment. The translocation of Bax to mitochondria was demonstrated, as well as the releasemore »« less
https://doi.org/10.1016/j.yexcr.2005.02.005
N,N-dimethyl phytosphingosine induces caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells

Journal Article Kim, Byeong Mo ; Choi, Yun Jung ; Han, Youngsoo ; ... - Toxicology and Applied Pharmacology

N,N-dimethyl phytosphingosine (DMPS) blocks the conversion of sphingosine to sphingosine-1-phosphate (S1P) by the enzyme sphingosine kinase (SK). In this study, we elucidated the apoptotic mechanisms of DMPS action on a human leukemia cell line using functional pharmacologic and genetic approaches. First, we demonstrated that DMPS-induced apoptosis is evidenced by nuclear morphological change, distinct internucleosomal DNA fragmentation, and an increased sub-G1 cell population. DMPS treatment led to the activation of caspase-9 and caspase-3, accompanied by the cleavage of poly(ADP-ribose) polymerase (PARP) and led to cytochrome c release, depolarization of the mitochondrial membrane potential, and downregulation of the anti-apoptotic members of themore »« less
https://doi.org/10.1016/j.taap.2009.05.020

Similar Records

Title: Zearalenone induces apoptosis of rat Sertoli cells through Fas‐Fas ligand and mitochondrial pathway

Abstract

Citation Formats

Involvement of cyclin D1 and E2f1 in zearalenone-induced DNA damage in testis of rats journal, November 2015

Crocin protects human embryonic kidney cells (HEK293) from α- and β-Zearalenol-induced ER stress and apoptosis journal, April 2016

Death Receptors: Signaling and Modulation journal, August 1998

Involvement of the Fas and Fas ligand in testicular germ cell apoptosis by zearalenone in rat journal, January 2010

A Novel Protein That Interacts with the Death Domain of Fas/APO1 Contains a Sequence Motif Related to the Death Domain journal, April 1995

Participation of the Fas-Signaling System in the Initiation of Germ Cell Apoptosis in Young Rat Testes after Exposure to Mono-(2-Ethylhexyl) Phthalate journal, November 1999

Influence of in vitro exposure to mycotoxin zearalenone and its derivatives on swine sperm quality journal, August 2008

Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease journal, December 2005

The TNF and TNF Receptor Superfamilies journal, February 2001

Zearalenone inhibits testosterone biosynthesis in mouse Leydig cells via the crosstalk of estrogen receptor signaling and orphan nuclear receptor Nur77 expression journal, June 2014

Exploration of Bcl-2 family and caspases-dependent apoptotic signaling pathway in Zearalenone-treated mouse endometrial stromal cells journal, August 2016

The mycotoxin Zearalenone induces apoptosis in human hepatocytes (HepG2) via p53-dependent mitochondrial signaling pathway journal, October 2008

The biochemistry of apoptosis journal, October 2000

Deletion of the BH1 Domain of Bcl-2 Accelerates Apoptosis by Acting in a Dominant Negative Fashion journal, March 2003

Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway journal, January 2012

Occurrence, detection and detoxification of mycotoxins journal, November 2015

Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells journal, March 2016

Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells journal, December 2010

Effects of PFNA exposure on expression of junction-associated molecules and secretory function in rat Sertoli cells journal, November 2010

Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis journal, March 2009

Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis journal, August 1998

Regulation of death receptor-induced apoptosis induced via CD95/Fas and other death receptors journal, February 2011

The influence of the mycotoxins deoxynivalenol and zearalenol on in vitro maturation of pig oocytes and in vitro culture of pig zygotes journal, December 2002

Involvement of the Fas/FasL pathway in the pathogenesis of germ cell tumours of the adult testis journal, January 2002

Involvement of MACH, a Novel MORT1/FADD-Interacting Protease, in Fas/APO-1- and TNF Receptor–Induced Cell Death journal, June 1996

Effect of lead on Sertoli–germ cell coculture of rat journal, July 2000

Programmed Cell Death in Animal Development journal, February 1997

Prognostic Significance of Soluble fas in the Serum of Patients with Bladder Cancer journal, August 1998

FADD, a novel death domain-containing protein, interacts with the death domain of fas and initiates apoptosis journal, May 1995

The influence of N-acetyl-l-cysteine on damage of porcine oocyte exposed to zearalenone in vitro journal, December 2015

Dose-response effects of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm journal, January 2011

Involvement of cyclin D1 and E2f1 in zearalenone-induced DNA damage in testis of rats
journal, November 2015

Crocin protects human embryonic kidney cells (HEK293) from α- and β-Zearalenol-induced ER stress and apoptosis
journal, April 2016

Death Receptors: Signaling and Modulation
journal, August 1998

Involvement of the Fas and Fas ligand in testicular germ cell apoptosis by zearalenone in rat
journal, January 2010

A Novel Protein That Interacts with the Death Domain of Fas/APO1 Contains a Sequence Motif Related to the Death Domain
journal, April 1995

Participation of the Fas-Signaling System in the Initiation of Germ Cell Apoptosis in Young Rat Testes after Exposure to Mono-(2-Ethylhexyl) Phthalate
journal, November 1999

Influence of in vitro exposure to mycotoxin zearalenone and its derivatives on swine sperm quality
journal, August 2008

Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease
journal, December 2005

The TNF and TNF Receptor Superfamilies
journal, February 2001

Zearalenone inhibits testosterone biosynthesis in mouse Leydig cells via the crosstalk of estrogen receptor signaling and orphan nuclear receptor Nur77 expression
journal, June 2014

Exploration of Bcl-2 family and caspases-dependent apoptotic signaling pathway in Zearalenone-treated mouse endometrial stromal cells
journal, August 2016

The mycotoxin Zearalenone induces apoptosis in human hepatocytes (HepG2) via p53-dependent mitochondrial signaling pathway
journal, October 2008

The biochemistry of apoptosis
journal, October 2000

Deletion of the BH1 Domain of Bcl-2 Accelerates Apoptosis by Acting in a Dominant Negative Fashion
journal, March 2003

Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway
journal, January 2012

Occurrence, detection and detoxification of mycotoxins
journal, November 2015

Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells
journal, March 2016

Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells
journal, December 2010

Effects of PFNA exposure on expression of junction-associated molecules and secretory function in rat Sertoli cells
journal, November 2010

Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis
journal, March 2009

Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis
journal, August 1998

Regulation of death receptor-induced apoptosis induced via CD95/Fas and other death receptors
journal, February 2011

The influence of the mycotoxins deoxynivalenol and zearalenol on in vitro maturation of pig oocytes and in vitro culture of pig zygotes
journal, December 2002

Involvement of the Fas/FasL pathway in the pathogenesis of germ cell tumours of the adult testis
journal, January 2002

Involvement of MACH, a Novel MORT1/FADD-Interacting Protease, in Fas/APO-1- and TNF Receptor–Induced Cell Death
journal, June 1996

Effect of lead on Sertoli–germ cell coculture of rat
journal, July 2000

Programmed Cell Death in Animal Development
journal, February 1997

Prognostic Significance of Soluble fas in the Serum of Patients with Bladder Cancer
journal, August 1998

FADD, a novel death domain-containing protein, interacts with the death domain of fas and initiates apoptosis
journal, May 1995

The influence of N-acetyl-l-cysteine on damage of porcine oocyte exposed to zearalenone in vitro
journal, December 2015

Dose-response effects of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm
journal, January 2011