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Title: Vitamin B12 added as a fortificant to flour retains high bioavailability when baked in bread

Abstract

Vitamin B12 deficiency and depletion are common world-wide, particularly in populations that consume low amounts of animal source foods. WHO and the Food Fortification Initiative recommend that wheat flour be fortified with vitamin B12 in regions where intake of B12 is low. The purpose of this pilot study in five participants was to determine if fortification of flour with B12 produced a bread product with intact B12 still present and to determine if healthy elderly absorb sufficient B12 from bread fortified in this manner. High-purity crystalline 14C-B12 was dissolved in water and added to flour (2 µg B12/100 g flour) in a bread maker and made into rolls (average 1.17 kBq (31.5 nCi) 14C-B12 in a total of 0.8 µg B12 per roll). Excess 14C first appeared in plasma 4 h after ingestion of the 14C fortified bread and plasma levels returned almost to background by 72 h. Measurement of 14C in plasma verified that the dose was absorbed into the systemic circulation. The cumulative % dose recovered in urine was 4.8–37.0% (mean = 20.1%). Most of the 14C label in the stool appeared by day 4, and the cumulative % dose recovered in stool was 24.5–43.0% (mean = 31.8%).more » Bioavailability among the 5 participants, calculated by subtracting the sum of urinary and fecal 14C excretion from the administered dose, was 28.4–63.7% (mean = 48.0%). This study showed that when B12 is added as a fortificant to flour it survives the fermentation and baking processes, and retains ~50% bioavailability when fed in small doses to healthy subjects. The Recommended Dietary Allowance of B12 for adults is 2.4 µg/d. This recommendation assumes that usual bioavailability of low doses of the vitamin in the crystalline form is 60%, while for the same amount in foods such as meat and fish it is 50%. Lastly, our pilot study shows that B12 added to bread as a fortificant in flour was absorbed as well as it is from endogenous food sources such as meat and fish.« less

Authors:
 [1];  [2];  [3];  [2];  [4];  [1]
  1. US Dept. of Agriculture (USDA)., Davis, CA (United States). ARS Western Human Nutrition Research Center
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Center for Accelerator Mass Spectrometry
  3. Rutgers Univ., New Brunswick, NJ (United States). School of Environmental and Biological Science
  4. Univ. of California, Davis, CA (United States). Dept. of Medical Pathology and Lab. Medicine
Publication Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
OSTI Identifier:
1488800
Report Number(s):
LLNL-JRNL-738621
Journal ID: ISSN 0168-583X; 891866
Grant/Contract Number:  
AC52-07NA27344
Resource Type:
Accepted Manuscript
Journal Name:
Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
Additional Journal Information:
Journal Volume: 438; Journal Issue: C; Journal ID: ISSN 0168-583X
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; Vitamin B12; Bioavailability; Fortification; Cobalamin; Vitamin pharmacokinetics

Citation Formats

Garrod, Marjorie G., Buchholz, Bruce A., Miller, Joshua W., Haack, Kurt W., Green, Ralph, and Allen, Lindsay H. Vitamin B12 added as a fortificant to flour retains high bioavailability when baked in bread. United States: N. p., 2018. Web. doi:10.1016/j.nimb.2018.05.042.
Garrod, Marjorie G., Buchholz, Bruce A., Miller, Joshua W., Haack, Kurt W., Green, Ralph, & Allen, Lindsay H. Vitamin B12 added as a fortificant to flour retains high bioavailability when baked in bread. United States. https://doi.org/10.1016/j.nimb.2018.05.042
Garrod, Marjorie G., Buchholz, Bruce A., Miller, Joshua W., Haack, Kurt W., Green, Ralph, and Allen, Lindsay H. Mon . "Vitamin B12 added as a fortificant to flour retains high bioavailability when baked in bread". United States. https://doi.org/10.1016/j.nimb.2018.05.042. https://www.osti.gov/servlets/purl/1488800.
@article{osti_1488800,
title = {Vitamin B12 added as a fortificant to flour retains high bioavailability when baked in bread},
author = {Garrod, Marjorie G. and Buchholz, Bruce A. and Miller, Joshua W. and Haack, Kurt W. and Green, Ralph and Allen, Lindsay H.},
abstractNote = {Vitamin B12 deficiency and depletion are common world-wide, particularly in populations that consume low amounts of animal source foods. WHO and the Food Fortification Initiative recommend that wheat flour be fortified with vitamin B12 in regions where intake of B12 is low. The purpose of this pilot study in five participants was to determine if fortification of flour with B12 produced a bread product with intact B12 still present and to determine if healthy elderly absorb sufficient B12 from bread fortified in this manner. High-purity crystalline 14C-B12 was dissolved in water and added to flour (2 µg B12/100 g flour) in a bread maker and made into rolls (average 1.17 kBq (31.5 nCi) 14C-B12 in a total of 0.8 µg B12 per roll). Excess 14C first appeared in plasma 4 h after ingestion of the 14C fortified bread and plasma levels returned almost to background by 72 h. Measurement of 14C in plasma verified that the dose was absorbed into the systemic circulation. The cumulative % dose recovered in urine was 4.8–37.0% (mean = 20.1%). Most of the 14C label in the stool appeared by day 4, and the cumulative % dose recovered in stool was 24.5–43.0% (mean = 31.8%). Bioavailability among the 5 participants, calculated by subtracting the sum of urinary and fecal 14C excretion from the administered dose, was 28.4–63.7% (mean = 48.0%). This study showed that when B12 is added as a fortificant to flour it survives the fermentation and baking processes, and retains ~50% bioavailability when fed in small doses to healthy subjects. The Recommended Dietary Allowance of B12 for adults is 2.4 µg/d. This recommendation assumes that usual bioavailability of low doses of the vitamin in the crystalline form is 60%, while for the same amount in foods such as meat and fish it is 50%. Lastly, our pilot study shows that B12 added to bread as a fortificant in flour was absorbed as well as it is from endogenous food sources such as meat and fish.},
doi = {10.1016/j.nimb.2018.05.042},
journal = {Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms},
number = C,
volume = 438,
place = {United States},
year = {2018},
month = {6}
}

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Figures / Tables:

Table 1 Table 1: Study subject characteristics and doses

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Works referenced in this record:

VITAMIN B12 DEFICIENCY IN THE ELDERLY
journal, July 1999


Plasma vitamin B-12 concentrations relate to intake source in the Framingham Offspring Study
journal, February 2000

  • Tucker, Katherine L.; Rich, Sharron; Rosenberg, Irwin
  • The American Journal of Clinical Nutrition, Vol. 71, Issue 2
  • DOI: 10.1093/ajcn/71.2.514

How common is vitamin B-12 deficiency?
journal, February 2009


Vitamin B12 deficiency
journal, June 2017

  • Green, Ralph; Allen, Lindsay H.; Bjørke-Monsen, Anne-Lise
  • Nature Reviews Disease Primers, Vol. 3, Issue 1
  • DOI: 10.1038/nrdp.2017.40

Human vitamin B12 absorption measurement by accelerator mass spectrometry using specifically labeled 14C-cobalamin
journal, April 2006

  • Carkeet, C.; Dueker, S. R.; Lango, J.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 15
  • DOI: 10.1073/pnas.0601251103

Considering the Case for Vitamin B12 Fortification of Flour
journal, March 2010

  • Allen, Lindsay H.; Rosenberg, Irwin H.; Oakley, Godfrey P.
  • Food and Nutrition Bulletin, Vol. 31, Issue 1_suppl1
  • DOI: 10.1177/15648265100311S104

Long-term kinetic study of β-carotene, using accelerator mass spectrometry in an adult volunteer
journal, November 2000


Absorption and retinol equivalence of β-carotene in humans is influenced by dietary vitamin A intake
journal, June 2003

  • Lemke, Shawna L.; Dueker, Stephen R.; Follett, Jennifer R.
  • Journal of Lipid Research, Vol. 44, Issue 8
  • DOI: 10.1194/jlr.M300116-JLR200

Quantitation of in vivo human folate metabolism
journal, September 2004

  • Lin, Yumei; Dueker, Stephen R.; Follett, Jennifer R.
  • The American Journal of Clinical Nutrition, Vol. 80, Issue 3
  • DOI: 10.1093/ajcn/80.3.680

Tips and traps in the 14C bio-AMS preparation laboratory
journal, October 2000

  • Buchholz, Bruce A.; Freeman, Stewart P. H. T.; Haack, Kurt W.
  • Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 172, Issue 1-4
  • DOI: 10.1016/S0168-583X(00)00142-7

Dual isotope test for assessing β-carotene cleavage to vitamin A in humans
journal, August 2002

  • Hickenbottom, Sabrina J.; Lemke, Shawna L.; Dueker, Stephen R.
  • European Journal of Nutrition, Vol. 41, Issue 4
  • DOI: 10.1007/s00394-002-0368-0

Accelerator Mass Spectrometry Can Be Used to Assess Vitamin A Metabolism Quantitatively in Boys in a Community Setting
journal, September 2010

  • Aklamati, Emmanuel K.; Mulenga, Modest; Dueker, Stephen R.
  • The Journal of Nutrition, Vol. 140, Issue 9
  • DOI: 10.3945/jn.110.125500

GABAA receptor target of tetramethylenedisulfotetramine
journal, May 2014

  • Zhao, C.; Hwang, S. H.; Buchholz, B. A.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 23
  • DOI: 10.1073/pnas.1407379111

Discussion Reporting of 14 C Data
journal, January 1977


Plasma, Tissue and Urinary Radioactivity after Oral Administration of56Co-labelled Vitamin B12
journal, July 1956


Reliability of the Dual-isotope Schilling Test for the Diagnosis of Pernicious Anemia or Malabsorption Syndrome
journal, May 1981

  • Domstad, Peggy A.; Choy, Young C.; Kim, E. Edmund
  • American Journal of Clinical Pathology, Vol. 75, Issue 5
  • DOI: 10.1093/ajcp/75.5.723

Iron, Zinc, Folate, and Vitamin B-12 Status Increased among Women and Children in Yaoundé and Douala, Cameroon, 1 Year after Introducing Fortified Wheat Flour
journal, June 2017

  • Engle-Stone, Reina; Nankap, Martin; Ndjebayi, Alex O.
  • The Journal of Nutrition, Vol. 147, Issue 7
  • DOI: 10.3945/jn.116.245076

Works referencing / citing this record:

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