Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids

Song, Yang; Wang, Mingming; Li, Suiqiong; Jin, Haibao; Cai, Xiaoli; Du, Dan; Li, He; Chen, Chun‐Long; Lin, Yuehe

doi:10.1002/smll.201803544

Title: Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids

Journal Article · 22 November 2018 · Small

DOI: https://doi.org/10.1002/smll.201803544 · OSTI ID:1488353

Song, Yang ^[1]; Wang, Mingming ^[2]; Li, Suiqiong ^[1]; Jin, Haibao ^[2]; Cai, Xiaoli ^[1]; Du, Dan ^[1]; Li, He ^[1]; Chen, Chun‐Long ^[2];

^[1]

Department of Mechanical and Materials Engineering Washington State University Pullman WA 99164 USA
Division of Physical Sciences Pacific Northwest National Laboratory Richland WA 99352 USA

Abstract The design and synthesis of biocompatible nanomaterials as cargoes for the intracellular delivery of therapeutic proteins or genes have attracted intense attention because of their potential for use in therapeutics. Despite the advances in this area, very few nanomaterials can be efficiently delivered to the cytosol. To address these challenges, crystalline nanoflower‐like particles are designed and synthesized from fluorinated sequence‐defined peptoids; the crystallinity and fluorination of these particles enable highly efficient cytosolic delivery with minimal cytotoxicity. A cytosol delivery rate of 80% has been achieved for the fluorinated peptoid nanoflowers. Furthermore, these nanocrystals can carry therapeutic genes, such as mRNA and effectively deliver the payload into the cytosol, demonstrating the universal delivery capability of the nanocrystals. The results indicate that self‐assembly of crystalline nanomaterials from fluorinated peptoids paves a new way toward development of nanocargoes with efficient cytosolic gene delivery capability.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830

OSTI ID:: 1488353

Journal Information:: Small, Journal Name: Small Journal Issue: 52 Vol. 14; ISSN 1613-6810

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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