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Title: Metabolomics and Isotope Tracing

We report great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. Additionally, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.
Authors:
 [1] ;  [1] ;  [1]
  1. Princeton Univ., NJ (United States)
Publication Date:
Grant/Contract Number:
SC0018420; SC0018260
Type:
Accepted Manuscript
Journal Name:
Cell
Additional Journal Information:
Journal Volume: 173; Journal Issue: 4; Journal ID: ISSN 0092-8674
Publisher:
Elsevier
Research Org:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1487276

Jang, Cholsoon, Chen, Li, and Rabinowitz, Joshua D. Metabolomics and Isotope Tracing. United States: N. p., Web. doi:10.1016/j.cell.2018.03.055.
Jang, Cholsoon, Chen, Li, & Rabinowitz, Joshua D. Metabolomics and Isotope Tracing. United States. doi:10.1016/j.cell.2018.03.055.
Jang, Cholsoon, Chen, Li, and Rabinowitz, Joshua D. 2018. "Metabolomics and Isotope Tracing". United States. doi:10.1016/j.cell.2018.03.055.
@article{osti_1487276,
title = {Metabolomics and Isotope Tracing},
author = {Jang, Cholsoon and Chen, Li and Rabinowitz, Joshua D.},
abstractNote = {We report great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. Additionally, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.},
doi = {10.1016/j.cell.2018.03.055},
journal = {Cell},
number = 4,
volume = 173,
place = {United States},
year = {2018},
month = {5}
}