Enzymatic synthesis of 1-caffeoylglycerol with deep eutectic solvent under continuous microflow conditions

Liu, Xi; Meng, Xiang-Yun; Xu, Yan; Dong, Tao; Zhang, Dong-Yang; Guan, Hui-Xiang; Zhuang, Yu; Wang, Jun

doi:10.1016/j.bej.2018.11.007

Title: Enzymatic synthesis of 1-caffeoylglycerol with deep eutectic solvent under continuous microflow conditions

Journal Article · 15 February 2019 · Biochemical Engineering Journal

DOI: https://doi.org/10.1016/j.bej.2018.11.007 · OSTI ID:1485770

Liu, Xi ^[1]; Meng, Xiang-Yun ^[1]; Xu, Yan ^[2]; Dong, Tao ^[3]; Zhang, Dong-Yang ^[2]; Guan, Hui-Xiang ^[1]; Zhuang, Yu ^[4]; Wang, Jun ^[2]

Jiangsu Univ. of Science and Technology, Zhenjiang (China)
Jiangsu Univ. of Science and Technology, Zhenjiang (China); Chinese Academy of Agricultural Sciences, Zhenjiang (China). Sericultural Research Inst.
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Nantong Yuanshengtang Biological Science and Technology Co., LTD, Haian (China)

1-caffeoylglycerol (1-CG) is a hydrophilic ester with a high solubility, and has a strong activity to prevent skin cancer caused by ultraviolet. However, the current preparation process to synthesize 1-CG via transesterification from methyl caffeate (MC) and glycerin was not economic due to long reaction time or adding extra field. Therefore, an enzymatic synthesis with deep eutectic solvent chloride-urea (ChCl-urea) in a microreactor was firstly applied under continuous microflow condition to improve the productivity. A maximum 1-CG yield of 96.49% was obtained under the optimized conditions: temperature of 65 degrees C, flow rate of 2 $$μ$$L/min, MC concentration of 50 g/L. Compared to the batch reactor, the reaction time reduced by 75%, the $$K_m$$ value decreased by 5/6, the reuse times of lipase increased by 2.29-fold, the external mass and transfer coefficients increased 10 times, which process was verified by numerical simulation. In addition, the produced 1-CG of 2 mg/mL has a high UV damage remediation ability of 75.16% using HaCaT cell model, which reveals 1-CG can repair the damage of cells radiated by the UV. Thus, this approach represents a convenient and cost-saving method to produce 1-CG using microfuidic biocatalysis.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1485770

Report Number(s):: NREL/JA-5100-72937

Journal Information:: Biochemical Engineering Journal, Journal Name: Biochemical Engineering Journal Journal Issue: C Vol. 142; ISSN 1369-703X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Deep eutectic solvents for biocatalytic transformations: focused lipase-catalyzed organic reactions Tan, Jia-Neng; Dou, Yuqing Applied Microbiology and Biotechnology, Vol. 104, Issue 4 https://doi.org/10.1007/s00253-019-10342-y	journal	January 2020
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Title: Enzymatic synthesis of 1-caffeoylglycerol with deep eutectic solvent under continuous microflow conditions

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