Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

doi:10.1039/c5ra23519g

Title: Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Abstract

Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.

Authors:

Tang, Yuting ^[1]; Zhang, Yue ^[2]; Rosenberg, Julian N. ^[2]; Sharif, Nadia ^[2]; Betenbaugh, Michael J. ^[2]; Wang, Fei ^[3]

Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals; Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals

Publication Date:: 2016-03-09

Research Org.:: Johns Hopkins Univ., Baltimore, MD (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1485584

Grant/Contract Number:: SC0012658

Resource Type:: Accepted Manuscript

Journal Name:: RSC Advances

Additional Journal Information:: Journal Volume: 6; Journal Issue: 35; Journal ID: ISSN 2046-2069

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats


                    Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., and Wang, Fei. Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE).  United States: N. p., 2016. 
        Web.  doi:10.1039/c5ra23519g.

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                    Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., & Wang, Fei. Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE).  United States.  doi:10.1039/c5ra23519g.

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                    Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., and Wang, Fei. Wed .  
        "Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)".  United States.  doi:10.1039/c5ra23519g.  https://www.osti.gov/servlets/purl/1485584.

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@article{osti_1485584,

  title        = {Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)},

  author       = {Tang, Yuting and Zhang, Yue and Rosenberg, Julian N. and Sharif, Nadia and Betenbaugh, Michael J. and Wang, Fei},

  abstractNote = {Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.},

  doi          = {10.1039/c5ra23519g},

  journal      = {RSC Advances},

  number       = 35,

  volume       = 6,

  place        = {United States},

  year         = {2016},

  month        = {3}

}

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DOI: 10.1039/c5ra23519g

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Cited by: 6 works

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Figures / Tables:

Fig. 1: Effects of reaction parameters on lipid recovery using the ASE method. (A) Effect of extraction solvents. Reaction conditions: extraction temperature of 100 °C, static time of 5 min, static cycle number of 4. (B) Effect of temperature. Reaction conditions: methanol/ chloroform (2 : 1, v/v), static time ofmore »

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Advances in microalgal lipid extraction for biofuel production: a review: Microalgal lipid extraction for biofuel production
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Harris, Jesse; Viner, Kelsey; Champagne, Pascale
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Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity
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Determination of Microalgal Lipid Content and Fatty Acid for Biofuel Production
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Title: Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Abstract

Citation Formats

Figures / Tables:

Removal of algae and turbidity by floating-media and sand filtration journal, August 2013

Biodiesel yields and fuel quality as criteria for algal-feedstock selection: Effects of CO2-supplementation and nutrient levels in cultures journal, March 2015

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures journal, February 2011

Extraction of Bio‐oils from Microalgae journal, December 2009

Lipid sovent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella journal, July 1988

Recent trends in the impurity profile of pharmaceuticals journal, January 2010

A biorefinery from Nannochloropsis sp. microalga – Extraction of oils and pigments. Production of biohydrogen from the leftover biomass journal, May 2013

Accelerated Solvent Extraction: A Technique for Sample Preparation journal, January 1996

Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Dunaliella salina and kavalactones from Piper methysticum journal, January 2004

Comparative Analyses of Three Chlorella Species in Response to Light and Sugar Reveal Distinctive Lipid Accumulation Patterns in the Microalga C. sorokiniana journal, April 2014

Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production journal, September 2014

Comparison of several methods for effective lipid extraction from microalgae journal, January 2010

Lipid extraction from biomass using co-solvent mixtures of ionic liquids and polar covalent molecules journal, March 2010

Pilot-scale supercritical carbon dioxide extractions for the recovery of triacylglycerols from microalgae: a practical tool for algal biofuels research journal, December 2011

Microalgae for biodiesel production and other applications: A review journal, January 2010

Effect of temperature and moisture on high pressure lipid/oil extraction from microalgae journal, December 2014

Microalgae as a raw material for biofuels production journal, November 2008

Extraction of hydrocarbon contamination from soils using accelerated solvent extraction journal, April 2000

Extraction of Lipids from Municipal Wastewater Plant Microorganisms for Production of Biodiesel journal, January 2007

Biodiesel from microalgae journal, May 2007

Effect of nitrogen limitation on cell growth, lipid accumulation and gene expression in Chlorella sorokiniana journal, June 2015

A comparative study on effective cell disruption methods for lipid extraction from microalgae: Effective cell disruption methods for lipid extraction journal, June 2011

High Photosynthetic Productivity of Green Microalga Chlorella sorokiniana journal, January 2000

The use of Design of Experiments and Response Surface Methodology to optimize biomass and lipid production by the oleaginous marine green alga, Nannochloropsis gaditana in response to light intensity, inoculum size and CO 2 journal, May 2015

Outdoor pilot-scale production of Nannochloropsis gaditana : Influence of culture parameters and lipid production rates in tubular photobioreactors journal, October 2014

Biodiesel quality and biochemical changes of microalgae Chlorella pyrenoidosa and Scenedesmus obliquus in response to nitrate levels journal, October 2014

Lipid storage compounds in raw activated sludge microorganisms for biofuels and oleochemicals production journal, January 2012

Investigation of high pressure steaming (HPS) as a thermal treatment for lipid extraction from Chlorella vulgaris journal, July 2014

A Rapid Method of Total Lipid Extraction and Purification journal, January 1959

Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark journal, February 2005

Evaluation of different solvent mixtures in esterifiable lipids extraction from microalgae Botryococcus braunii for biodiesel production journal, February 2016

Trichosarcina polymorpha Gen. et Sp. Nov. journal, March 1965

Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae journal, July 2011

Comparison of biomass and lipid production under ambient carbon dioxide vigorous aeration and 3% carbon dioxide condition among the lead candidate Chlorella strains screened by various photobioreactor scales journal, December 2015

Influence of fatty acid composition of raw materials on biodiesel properties journal, January 2009

Optimization of accelerated solvent extraction of antioxidants from Spirulina platensis microalga journal, December 2005

Ionic liquid-mediated extraction of lipids from algal biomass journal, April 2012

Pressurized fluid extraction of polyunsaturated fatty acids from the microalga Nannochloropsis oculata journal, December 2012

Accelerated solvent extraction of lipids for determining the fatty acid composition of biological material journal, January 1998

Optimization of an Oil Extraction Process for Algae from the Treatment of Manure Effluent journal, July 2009

Advances in microalgal lipid extraction for biofuel production: a review: Microalgal lipid extraction for biofuel production journal, August 2018

Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity journal, November 2019

Determination of Microalgal Lipid Content and Fatty Acid for Biofuel Production journal, January 2018

Removal of algae and turbidity by floating-media and sand filtration
journal, August 2013

Biodiesel yields and fuel quality as criteria for algal-feedstock selection: Effects of CO2-supplementation and nutrient levels in cultures
journal, March 2015

Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures
journal, February 2011

Extraction of Bio‐oils from Microalgae
journal, December 2009

Lipid sovent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella
journal, July 1988

Recent trends in the impurity profile of pharmaceuticals
journal, January 2010

A biorefinery from Nannochloropsis sp. microalga – Extraction of oils and pigments. Production of biohydrogen from the leftover biomass
journal, May 2013

Accelerated Solvent Extraction: A Technique for Sample Preparation
journal, January 1996

Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Dunaliella salina and kavalactones from Piper methysticum
journal, January 2004

Comparative Analyses of Three Chlorella Species in Response to Light and Sugar Reveal Distinctive Lipid Accumulation Patterns in the Microalga C. sorokiniana
journal, April 2014

Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production
journal, September 2014

Comparison of several methods for effective lipid extraction from microalgae
journal, January 2010

Lipid extraction from biomass using co-solvent mixtures of ionic liquids and polar covalent molecules
journal, March 2010

Pilot-scale supercritical carbon dioxide extractions for the recovery of triacylglycerols from microalgae: a practical tool for algal biofuels research
journal, December 2011

Microalgae for biodiesel production and other applications: A review
journal, January 2010

Effect of temperature and moisture on high pressure lipid/oil extraction from microalgae
journal, December 2014

Microalgae as a raw material for biofuels production
journal, November 2008

Extraction of hydrocarbon contamination from soils using accelerated solvent extraction
journal, April 2000

Extraction of Lipids from Municipal Wastewater Plant Microorganisms for Production of Biodiesel
journal, January 2007

Biodiesel from microalgae
journal, May 2007

Effect of nitrogen limitation on cell growth, lipid accumulation and gene expression in Chlorella sorokiniana
journal, June 2015

A comparative study on effective cell disruption methods for lipid extraction from microalgae: Effective cell disruption methods for lipid extraction
journal, June 2011

High Photosynthetic Productivity of Green Microalga Chlorella sorokiniana
journal, January 2000

The use of Design of Experiments and Response Surface Methodology to optimize biomass and lipid production by the oleaginous marine green alga, Nannochloropsis gaditana in response to light intensity, inoculum size and CO 2
journal, May 2015

Outdoor pilot-scale production of Nannochloropsis gaditana : Influence of culture parameters and lipid production rates in tubular photobioreactors
journal, October 2014

Biodiesel quality and biochemical changes of microalgae Chlorella pyrenoidosa and Scenedesmus obliquus in response to nitrate levels
journal, October 2014

Lipid storage compounds in raw activated sludge microorganisms for biofuels and oleochemicals production
journal, January 2012

Investigation of high pressure steaming (HPS) as a thermal treatment for lipid extraction from Chlorella vulgaris
journal, July 2014

A Rapid Method of Total Lipid Extraction and Purification
journal, January 1959

Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark
journal, February 2005

Evaluation of different solvent mixtures in esterifiable lipids extraction from microalgae Botryococcus braunii for biodiesel production
journal, February 2016

Trichosarcina polymorpha Gen. et Sp. Nov.
journal, March 1965

Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae
journal, July 2011

Comparison of biomass and lipid production under ambient carbon dioxide vigorous aeration and 3% carbon dioxide condition among the lead candidate Chlorella strains screened by various photobioreactor scales
journal, December 2015

Influence of fatty acid composition of raw materials on biodiesel properties
journal, January 2009

Optimization of accelerated solvent extraction of antioxidants from Spirulina platensis microalga
journal, December 2005

Ionic liquid-mediated extraction of lipids from algal biomass
journal, April 2012

Pressurized fluid extraction of polyunsaturated fatty acids from the microalga Nannochloropsis oculata
journal, December 2012

Accelerated solvent extraction of lipids for determining the fatty acid composition of biological material
journal, January 1998

Optimization of an Oil Extraction Process for Algae from the Treatment of Manure Effluent
journal, July 2009

Advances in microalgal lipid extraction for biofuel production: a review: Microalgal lipid extraction for biofuel production
journal, August 2018

Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity
journal, November 2019

Determination of Microalgal Lipid Content and Fatty Acid for Biofuel Production
journal, January 2018