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Title: Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Abstract

Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.

Authors:
 [1];  [2];  [2];  [2];  [2];  [3]
  1. Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals; Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
  2. Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
  3. Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals
Publication Date:
Research Org.:
Johns Hopkins Univ., Baltimore, MD (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1485584
Grant/Contract Number:  
SC0012658
Resource Type:
Accepted Manuscript
Journal Name:
RSC Advances
Additional Journal Information:
Journal Volume: 6; Journal Issue: 35; Journal ID: ISSN 2046-2069
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., and Wang, Fei. Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE). United States: N. p., 2016. Web. doi:10.1039/c5ra23519g.
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., & Wang, Fei. Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE). United States. doi:10.1039/c5ra23519g.
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., and Wang, Fei. Wed . "Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)". United States. doi:10.1039/c5ra23519g. https://www.osti.gov/servlets/purl/1485584.
@article{osti_1485584,
title = {Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)},
author = {Tang, Yuting and Zhang, Yue and Rosenberg, Julian N. and Sharif, Nadia and Betenbaugh, Michael J. and Wang, Fei},
abstractNote = {Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.},
doi = {10.1039/c5ra23519g},
journal = {RSC Advances},
number = 35,
volume = 6,
place = {United States},
year = {2016},
month = {3}
}

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Cited by: 6 works
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Figures / Tables:

Fig. 1 Fig. 1: Effects of reaction parameters on lipid recovery using the ASE method. (A) Effect of extraction solvents. Reaction conditions: extraction temperature of 100 °C, static time of 5 min, static cycle number of 4. (B) Effect of temperature. Reaction conditions: methanol/ chloroform (2 : 1, v/v), static time ofmore » 5 min, static cycle number of 4. (C) Effect of static time. Reaction conditions: methanol/chloroform (2 : 1, v/v), extraction temperature of 100 °C, static cycle number of 4. (D) Effect of static cycles. Reaction conditions: methanol/chloroform (2 : 1, v/v), extraction temperature of 100 °C, static time of 5 min. Error bars designate standard deviation from the average of duplicates.« less

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    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.