Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)
Abstract
Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.
- Authors:
-
- Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals; Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
- Johns Hopkins Univ., Baltimore, MD (United States). Department of Chemical & Biomolecular Engineering
- Nanjing Forestry Univ., Nanjing (China). College of Chemical Engineering, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals
- Publication Date:
- Research Org.:
- Johns Hopkins Univ., Baltimore, MD (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1485584
- Grant/Contract Number:
- SC0012658
- Resource Type:
- Accepted Manuscript
- Journal Name:
- RSC Advances
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 35; Journal ID: ISSN 2046-2069
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., and Wang, Fei. Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE). United States: N. p., 2016.
Web. doi:10.1039/c5ra23519g.
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., & Wang, Fei. Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE). United States. https://doi.org/10.1039/c5ra23519g
Tang, Yuting, Zhang, Yue, Rosenberg, Julian N., Sharif, Nadia, Betenbaugh, Michael J., and Wang, Fei. Wed .
"Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)". United States. https://doi.org/10.1039/c5ra23519g. https://www.osti.gov/servlets/purl/1485584.
@article{osti_1485584,
title = {Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)},
author = {Tang, Yuting and Zhang, Yue and Rosenberg, Julian N. and Sharif, Nadia and Betenbaugh, Michael J. and Wang, Fei},
abstractNote = {Accelerated solvent extraction (ASE), a commercially available pressurized fluid extraction technique and conventional manual extraction were compared to identify the most effective chloroform–methanol extraction method for algal lipids. Using optimal ASE operating conditions (methanol/chloroform = 2 : 1 by vol, 100 °C, static time of 5 min, and four static cycles), the lipid contents of Chlorella vulgaris, C. sorokiniana, C. zofingiensis and Nannochloropsis gaditana were 27.5%, 25.8%, 15.2%, 29.8% of dry biomass, respectively. Here, the total fatty acid methyl ester (FAME) content of dry biomass from ASE extraction was found to be 1.3–2.7 fold higher than that from conventional manual extract from these species, demonstrating that ASE exhibited significant improvement for lipid and FAME recovery. Furthermore, ASE showed the capacity to extract all-cis-5,8,11,14,17-eicosapentaenoic acid (EPA) as 3.0% of dry biomass from Nannochloropsis gaditana suggesting that ASE has the potential to obtain polyunsaturated fatty acids (PUFAs) as well.},
doi = {10.1039/c5ra23519g},
journal = {RSC Advances},
number = 35,
volume = 6,
place = {United States},
year = {2016},
month = {3}
}
Web of Science
Figures / Tables:

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