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Title: Exploring variation in phyllosphere microbial communities across four hemlock species

Abstract

Here, the hemlock woolly adelgid (HWA) is an insect native to Asia and likely western North America. First reported in eastern North America in 1951, it has devastated eastern hemlock ( Tsuga canadensis) populations. Loss of hemlock will greatly affect the structure and function of eastern forests. Susceptibility to adelgid infestation varies within eastern hemlocks and across other hemlock species. Our study was conducted to determine whether eastern hemlocks share a similar stem (phyllosphere) microbial community with other co–occurring hemlocks and whether community–level shifts are associated within trees of the same species based on HWA infestation. Surprisingly, we found no difference in microbial community composition or diversity between trees of the same species based on the level of HWA infestation. However, microbial communities varied significantly across the four hemlock trees sampled, native T. canadensis and three non–natives: Tsuga chinensis, Tsuga dumosa, and Tsuga sieboldii. Within these tree hosts, microbial communities from T. dumosa and T. chinensis clustered together, and microbial communities from T. canadensis and T. sieboldii clustered separately from all other tree species. Additionally, specific indicator taxa were identified for all the tree species sampled. These results indicate that Asian hemlocks might not fill the same niche in easternmore » forests as the native eastern hemlock. Further work should be conducted to determine how differences in hemlock species and associated microbial communities might scale up to alter organismal interactions involving hemlocks.« less

Authors:
 [1];  [2];  [2];  [2];  [3];  [3];  [2]; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. North Carolina State Univ., Mills River, NC (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1485188
Alternate Identifier(s):
OSTI ID: 1485189; OSTI ID: 1491337
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Ecosphere
Additional Journal Information:
Journal Volume: 9; Journal Issue: 12; Journal ID: ISSN 2150-8925
Publisher:
Ecological Society of America
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; hemlock; hemlock woolly adelgid; microbial community; next‐generation sequencing; phyllosphere

Citation Formats

Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, and Cregger, Melissa A. Exploring variation in phyllosphere microbial communities across four hemlock species. United States: N. p., 2018. Web. doi:10.1002/ecs2.2524.
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, & Cregger, Melissa A. Exploring variation in phyllosphere microbial communities across four hemlock species. United States. doi:10.1002/ecs2.2524.
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, and Cregger, Melissa A. Mon . "Exploring variation in phyllosphere microbial communities across four hemlock species". United States. doi:10.1002/ecs2.2524.
@article{osti_1485188,
title = {Exploring variation in phyllosphere microbial communities across four hemlock species},
author = {Rogers, Timothy J. and Leppanen, Christy and Brown, Veronica and Fordyce, James A. and LeBude, Anthony and Ranney, Thomas and Simberloff, Daniel and Cregger, Melissa A.},
abstractNote = {Here, the hemlock woolly adelgid (HWA) is an insect native to Asia and likely western North America. First reported in eastern North America in 1951, it has devastated eastern hemlock (Tsuga canadensis) populations. Loss of hemlock will greatly affect the structure and function of eastern forests. Susceptibility to adelgid infestation varies within eastern hemlocks and across other hemlock species. Our study was conducted to determine whether eastern hemlocks share a similar stem (phyllosphere) microbial community with other co–occurring hemlocks and whether community–level shifts are associated within trees of the same species based on HWA infestation. Surprisingly, we found no difference in microbial community composition or diversity between trees of the same species based on the level of HWA infestation. However, microbial communities varied significantly across the four hemlock trees sampled, native T. canadensis and three non–natives: Tsuga chinensis, Tsuga dumosa, and Tsuga sieboldii. Within these tree hosts, microbial communities from T. dumosa and T. chinensis clustered together, and microbial communities from T. canadensis and T. sieboldii clustered separately from all other tree species. Additionally, specific indicator taxa were identified for all the tree species sampled. These results indicate that Asian hemlocks might not fill the same niche in eastern forests as the native eastern hemlock. Further work should be conducted to determine how differences in hemlock species and associated microbial communities might scale up to alter organismal interactions involving hemlocks.},
doi = {10.1002/ecs2.2524},
journal = {Ecosphere},
number = 12,
volume = 9,
place = {United States},
year = {2018},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/ecs2.2524

Citation Metrics:
Cited by: 2 works
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Figures / Tables:

Fig. 1 Fig. 1: Distance-based redundancy analysis plots of (a) bacterial and (b) fungal communities across four hemlock species.

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      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.