Exploring variation in phyllosphere microbial communities across four hemlock species
Abstract
Abstract The hemlock woolly adelgid (HWA) is an insect native to Asia and likely western North America. First reported in eastern North America in 1951, it has devastated eastern hemlock ( Tsuga canadensis ) populations. Loss of hemlock will greatly affect the structure and function of eastern forests. Susceptibility to adelgid infestation varies within eastern hemlocks and across other hemlock species. Our study was conducted to determine whether eastern hemlocks share a similar stem (phyllosphere) microbial community with other co‐occurring hemlocks and whether community‐level shifts are associated within trees of the same species based on HWA infestation. Surprisingly, we found no difference in microbial community composition or diversity between trees of the same species based on the level of HWA infestation. However, microbial communities varied significantly across the four hemlock trees sampled, native T. canadensis and three non‐natives: Tsuga chinensis , Tsuga dumosa , and Tsuga sieboldii . Within these tree hosts, microbial communities from T. dumosa and T. chinensis clustered together, and microbial communities from T. canadensis and T. sieboldii clustered separately from all other tree species. Additionally, specific indicator taxa were identified for all the tree species sampled. These results indicate that Asian hemlocks might not fill the same niche in eastern forestsmore »
- Authors:
-
- Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA, Department of Ecology and Evolutionary Biology University of Tennessee Knoxville Tennessee 37996 USA
- Department of Ecology and Evolutionary Biology University of Tennessee Knoxville Tennessee 37996 USA
- Department of Horticultural Science North Carolina State University Mills River North Carolina 28759 USA
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1485188
- Alternate Identifier(s):
- OSTI ID: 1485189; OSTI ID: 1491337
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Ecosphere
- Additional Journal Information:
- Journal Name: Ecosphere Journal Volume: 9 Journal Issue: 12; Journal ID: ISSN 2150-8925
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; hemlock; hemlock woolly adelgid; microbial community; next‐generation sequencing; phyllosphere
Citation Formats
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, and Cregger, Melissa A. Exploring variation in phyllosphere microbial communities across four hemlock species. United States: N. p., 2018.
Web. doi:10.1002/ecs2.2524.
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, & Cregger, Melissa A. Exploring variation in phyllosphere microbial communities across four hemlock species. United States. https://doi.org/10.1002/ecs2.2524
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, and Cregger, Melissa A. Mon .
"Exploring variation in phyllosphere microbial communities across four hemlock species". United States. https://doi.org/10.1002/ecs2.2524.
@article{osti_1485188,
title = {Exploring variation in phyllosphere microbial communities across four hemlock species},
author = {Rogers, Timothy J. and Leppanen, Christy and Brown, Veronica and Fordyce, James A. and LeBude, Anthony and Ranney, Thomas and Simberloff, Daniel and Cregger, Melissa A.},
abstractNote = {Abstract The hemlock woolly adelgid (HWA) is an insect native to Asia and likely western North America. First reported in eastern North America in 1951, it has devastated eastern hemlock ( Tsuga canadensis ) populations. Loss of hemlock will greatly affect the structure and function of eastern forests. Susceptibility to adelgid infestation varies within eastern hemlocks and across other hemlock species. Our study was conducted to determine whether eastern hemlocks share a similar stem (phyllosphere) microbial community with other co‐occurring hemlocks and whether community‐level shifts are associated within trees of the same species based on HWA infestation. Surprisingly, we found no difference in microbial community composition or diversity between trees of the same species based on the level of HWA infestation. However, microbial communities varied significantly across the four hemlock trees sampled, native T. canadensis and three non‐natives: Tsuga chinensis , Tsuga dumosa , and Tsuga sieboldii . Within these tree hosts, microbial communities from T. dumosa and T. chinensis clustered together, and microbial communities from T. canadensis and T. sieboldii clustered separately from all other tree species. Additionally, specific indicator taxa were identified for all the tree species sampled. These results indicate that Asian hemlocks might not fill the same niche in eastern forests as the native eastern hemlock. Further work should be conducted to determine how differences in hemlock species and associated microbial communities might scale up to alter organismal interactions involving hemlocks.},
doi = {10.1002/ecs2.2524},
journal = {Ecosphere},
number = 12,
volume = 9,
place = {United States},
year = {Mon Dec 10 00:00:00 EST 2018},
month = {Mon Dec 10 00:00:00 EST 2018}
}
https://doi.org/10.1002/ecs2.2524
Web of Science
Figures / Tables:
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