DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Exploring variation in phyllosphere microbial communities across four hemlock species

Abstract

Abstract The hemlock woolly adelgid (HWA) is an insect native to Asia and likely western North America. First reported in eastern North America in 1951, it has devastated eastern hemlock ( Tsuga canadensis ) populations. Loss of hemlock will greatly affect the structure and function of eastern forests. Susceptibility to adelgid infestation varies within eastern hemlocks and across other hemlock species. Our study was conducted to determine whether eastern hemlocks share a similar stem (phyllosphere) microbial community with other co‐occurring hemlocks and whether community‐level shifts are associated within trees of the same species based on HWA infestation. Surprisingly, we found no difference in microbial community composition or diversity between trees of the same species based on the level of HWA infestation. However, microbial communities varied significantly across the four hemlock trees sampled, native T. canadensis and three non‐natives: Tsuga chinensis , Tsuga dumosa , and Tsuga sieboldii . Within these tree hosts, microbial communities from T. dumosa and T. chinensis clustered together, and microbial communities from T. canadensis and T. sieboldii clustered separately from all other tree species. Additionally, specific indicator taxa were identified for all the tree species sampled. These results indicate that Asian hemlocks might not fill the same niche in eastern forestsmore » as the native eastern hemlock. Further work should be conducted to determine how differences in hemlock species and associated microbial communities might scale up to alter organismal interactions involving hemlocks.« less

Authors:
 [1];  [2];  [2];  [2];  [3];  [3];  [2];  [1]
  1. Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA, Department of Ecology and Evolutionary Biology University of Tennessee Knoxville Tennessee 37996 USA
  2. Department of Ecology and Evolutionary Biology University of Tennessee Knoxville Tennessee 37996 USA
  3. Department of Horticultural Science North Carolina State University Mills River North Carolina 28759 USA
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1485188
Alternate Identifier(s):
OSTI ID: 1485189; OSTI ID: 1491337
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Ecosphere
Additional Journal Information:
Journal Name: Ecosphere Journal Volume: 9 Journal Issue: 12; Journal ID: ISSN 2150-8925
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; hemlock; hemlock woolly adelgid; microbial community; next‐generation sequencing; phyllosphere

Citation Formats

Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, and Cregger, Melissa A. Exploring variation in phyllosphere microbial communities across four hemlock species. United States: N. p., 2018. Web. doi:10.1002/ecs2.2524.
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, & Cregger, Melissa A. Exploring variation in phyllosphere microbial communities across four hemlock species. United States. https://doi.org/10.1002/ecs2.2524
Rogers, Timothy J., Leppanen, Christy, Brown, Veronica, Fordyce, James A., LeBude, Anthony, Ranney, Thomas, Simberloff, Daniel, and Cregger, Melissa A. Mon . "Exploring variation in phyllosphere microbial communities across four hemlock species". United States. https://doi.org/10.1002/ecs2.2524.
@article{osti_1485188,
title = {Exploring variation in phyllosphere microbial communities across four hemlock species},
author = {Rogers, Timothy J. and Leppanen, Christy and Brown, Veronica and Fordyce, James A. and LeBude, Anthony and Ranney, Thomas and Simberloff, Daniel and Cregger, Melissa A.},
abstractNote = {Abstract The hemlock woolly adelgid (HWA) is an insect native to Asia and likely western North America. First reported in eastern North America in 1951, it has devastated eastern hemlock ( Tsuga canadensis ) populations. Loss of hemlock will greatly affect the structure and function of eastern forests. Susceptibility to adelgid infestation varies within eastern hemlocks and across other hemlock species. Our study was conducted to determine whether eastern hemlocks share a similar stem (phyllosphere) microbial community with other co‐occurring hemlocks and whether community‐level shifts are associated within trees of the same species based on HWA infestation. Surprisingly, we found no difference in microbial community composition or diversity between trees of the same species based on the level of HWA infestation. However, microbial communities varied significantly across the four hemlock trees sampled, native T. canadensis and three non‐natives: Tsuga chinensis , Tsuga dumosa , and Tsuga sieboldii . Within these tree hosts, microbial communities from T. dumosa and T. chinensis clustered together, and microbial communities from T. canadensis and T. sieboldii clustered separately from all other tree species. Additionally, specific indicator taxa were identified for all the tree species sampled. These results indicate that Asian hemlocks might not fill the same niche in eastern forests as the native eastern hemlock. Further work should be conducted to determine how differences in hemlock species and associated microbial communities might scale up to alter organismal interactions involving hemlocks.},
doi = {10.1002/ecs2.2524},
journal = {Ecosphere},
number = 12,
volume = 9,
place = {United States},
year = {Mon Dec 10 00:00:00 EST 2018},
month = {Mon Dec 10 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/ecs2.2524

Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

Figures / Tables:

Fig. 1 Fig. 1: Distance-based redundancy analysis plots of (a) bacterial and (b) fungal communities across four hemlock species.

Save / Share:

Works referenced in this record:

Cutadapt removes adapter sequences from high-throughput sequencing reads
journal, May 2011


Associations between species and groups of sites: indices and statistical inference
journal, December 2009

  • Cáceres, Miquel De; Legendre, Pierre
  • Ecology, Vol. 90, Issue 12
  • DOI: 10.1890/08-1823.1

Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
journal, July 2006

  • DeSantis, T. Z.; Hugenholtz, P.; Larsen, N.
  • Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5069-5072
  • DOI: 10.1128/AEM.03006-05

Tree phyllosphere bacterial communities: exploring the magnitude of intra- and inter-individual variation among host species
journal, January 2016

  • Laforest-Lapointe, Isabelle; Messier, Christian; Kembel, Steven W.
  • PeerJ, Vol. 4
  • DOI: 10.7717/peerj.2367

Growth and Infestation by Hemlock Woolly Adelgid of Two Exotic Hemlock Species in a New England Forest
journal, May 2008


Importance of species interactions to community heritability: a genetic basis to trophic-level interactions
journal, November 2005


Ancient and modern colonization of North America by hemlock woolly adelgid, Adelges tsugae (Hemiptera: Adelgidae), an invasive insect from East Asia
journal, March 2016

  • Havill, Nathan P.; Shiyake, Shigehiko; Lamb Galloway, Ashley
  • Molecular Ecology, Vol. 25, Issue 9
  • DOI: 10.1111/mec.13589

Acremonium Endophyte Interactions with Enhanced Plant Resistance to Insects
journal, January 1994


Evaluation of Hemlock (<I>Tsuga</I>) Species and Hybrids for Resistance to <I>Adelges tsugae</I> (Hemiptera: Adelgidae) Using Artificial Infestation
journal, June 2009

  • Montgomery, Michael E.; Bentz, S. E.; Olsen, Richard T.
  • Journal of Economic Entomology, Vol. 102, Issue 3
  • DOI: 10.1603/029.102.0351

Fungal Endophytes in Stems and Leaves: From Latent Pathogen to Mutualistic Symbiont
journal, February 1988


Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum
journal, September 2007

  • Ventura, M.; Canchaya, C.; Tauch, A.
  • Microbiology and Molecular Biology Reviews, Vol. 71, Issue 3
  • DOI: 10.1128/MMBR.00005-07

Terpene Chemistry of Eastern Hemlocks Resistant to Hemlock Woolly Adelgid
journal, September 2014

  • McKenzie, E. Alexa; Elkinton, Joseph S.; Casagrande, Richard A.
  • Journal of Chemical Ecology, Vol. 40, Issue 9
  • DOI: 10.1007/s10886-014-0495-0

Degradation of Pollen by Phycomycetes
journal, July 1960


Community Heritability Measures the Evolutionary Consequences of Indirect Genetic Effects on Community Structure
journal, May 2006


The effect of fungi on the interaction between host plants and their herbivores
journal, December 1987


Plant Genetics Affects Arthropod Community Richness and Composition: Evidence from a Synthetic Eucalypt Hybrid Population
journal, December 2000


Host species identity, site and time drive temperate tree phyllosphere bacterial community structure
journal, June 2016

  • Laforest-Lapointe, Isabelle; Messier, Christian; Kembel, Steven W.
  • Microbiome, Vol. 4, Issue 1
  • DOI: 10.1186/s40168-016-0174-1

Microbiology of the Phyllosphere
journal, April 2003


Resilience of the Natural Phyllosphere Microbiota of the Grapevine to Chemical and Biological Pesticides
journal, March 2014

  • Perazzolli, Michele; Antonielli, Livio; Storari, Michelangelo
  • Applied and Environmental Microbiology, Vol. 80, Issue 12
  • DOI: 10.1128/AEM.00415-14

Plant Genetic Determinants of Arthropod Community Structure and Diversity
journal, January 2005


Fungal community on decomposing leaf litter undergoes rapid successional changes
journal, October 2012


Bacterial Community Assemblages Associated with the Phyllosphere, Dermosphere, and Rhizosphere of Tree Species of the Atlantic Forest are Host Taxon Dependent
journal, June 2014

  • Lambais, Marcio R.; Lucheta, Adriano R.; Crowley, David E.
  • Microbial Ecology, Vol. 68, Issue 3
  • DOI: 10.1007/s00248-014-0433-2

Small mammal habitat associations in poletimber and sawtimber stands of four forest cover types
journal, December 1991

  • DeGraaf, Richard M.; Snyder, Dana P.; Hill, Barbara J.
  • Forest Ecology and Management, Vol. 46, Issue 3-4
  • DOI: 10.1016/0378-1127(91)90234-M

Hemlock Woolly Adelgid in New England Forests: Canopy Impacts Transforming Ecosystem Processes and Landscapes
journal, April 2005


Improving indicator species analysis by combining groups of sites
journal, September 2010


Relationships between phyllosphere bacterial communities and plant functional traits in a neotropical forest
journal, September 2014

  • Kembel, S. W.; O'Connor, T. K.; Arnold, H. K.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 38
  • DOI: 10.1073/pnas.1216057111

Foliar Chemistry Linked to Infestation and Susceptibility to Hemlock Woolly Adelgid (Homoptera: Adelgidae)
journal, February 2006

  • Pontius, Jennifer A.; Hallett, Richard A.; Jenkins, Jennifer C.
  • Environmental Entomology, Vol. 35, Issue 1
  • DOI: 10.1603/0046-225X-35.1.112

Friedmanniella endophytica sp. nov., an endophytic actinobacterium isolated from bark of Kandelia candel
journal, August 2016

  • Tuo, Li; Pan, Zhen; Li, Fei-Na
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 66, Issue 8
  • DOI: 10.1099/ijsem.0.001146

A Comparison of Arborists’ and Horticulturists’ Preferences of Tsuga chinensis to T. canadensis in the Urban Landscape
journal, January 2015

  • Dampier, Jason; Harper, Richard; Schwartzberg, Lora
  • Arboriculture & Urban Forestry, Vol. 41, Issue 1
  • DOI: 10.48044/jauf.2015.005

Loss of foundation species: consequences for the structure and dynamics of forested ecosystems
journal, November 2005


Phyllosphere microbiology with special reference to diversity and plant genotype
journal, December 2008


Mitochondrial DNA from Hemlock Woolly Adelgid (Hemiptera: Adelgidae) Suggests Cryptic Speciation and Pinpoints the Source of the Introduction to Eastern North America
journal, March 2006


Ecological Implications of Anti-Pathogen Effects of Tropical Fungal Endophytes and Mycorrhizae
journal, March 2007

  • Herre, Edward Allen; Mejía, Luis C.; Kyllo, Damond A.
  • Ecology, Vol. 88, Issue 3
  • DOI: 10.1890/05-1606

Comparison of Microbial Community Structure in Kiwifruit Pollens
journal, April 2018


The Populus holobiont: dissecting the effects of plant niches and genotype on the microbiome
journal, February 2018


Microbial nitrification in throughfall of a Japanese cedar associated with archaea from the tree canopy
journal, September 2016


Dispersal of Bacillus subtilis and its effect on strawberry phyllosphere microbiota under open field and protection conditions
journal, March 2016

  • Wei, Feng; Hu, Xiaoping; Xu, Xiangming
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep22611

Entropy and diversity
journal, May 2006


Protection of Arabidopsis thaliana against Leaf-Pathogenic Pseudomonas syringae by Sphingomonas Strains in a Controlled Model System
journal, March 2011

  • Innerebner, Gerd; Knief, Claudia; Vorholt, Julia A.
  • Applied and Environmental Microbiology, Vol. 77, Issue 10
  • DOI: 10.1128/AEM.00133-11

Plant traits and taxonomy drive host associations in tropical phyllosphere fungal communities
journal, April 2014


Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi
journal, January 2005


Endophytic fungi as biocontrol agents of Theobroma cacao pathogens
journal, July 2008


Geographical Location Determines the Population Structure in Phyllosphere Microbial Communities of a Salt-Excreting Desert Tree
journal, September 2011

  • Finkel, Omri M.; Burch, Adrien Y.; Lindow, Steven E.
  • Applied and Environmental Microbiology, Vol. 77, Issue 21
  • DOI: 10.1128/AEM.05565-11

Hemlock woolly adelgid impacts on community structure and N cycling rates in eastern hemlock forests
journal, May 1999

  • Jenkins, Jennifer C.; Aber, John D.; Canham, Charles D.
  • Canadian Journal of Forest Research, Vol. 29, Issue 5
  • DOI: 10.1139/x99-034

UNITE: a database providing web-based methods for the molecular identification of ectomycorrhizal fungi: Methods
journal, March 2005


Phyllosphere microbiology
journal, June 2002


Ecology of Actinomycetes
journal, October 1983


The Geographical and Ecological Distribution of Arboreal Psocoptera
journal, January 1985


Plant Genotype and Environment Interact to Shape a Diverse Arthropod Community on Evening Primrose (Oenothera Biennis)
journal, April 2005

  • Johnson, Marc T. J.; Agrawal, Anurag A.
  • Ecology, Vol. 86, Issue 4
  • DOI: 10.1890/04-1068

Figures / Tables found in this record:

    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.