DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short‐lifetime near‐infrared dyes and time‐gated imaging

ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [3]
  1. Department of Biomedical Engineering Rensselaer Polytechnic Institute Troy New York
  2. Department of Molecular and Cellular Physiology Albany Medical College Albany New York
  3. Department of Chemistry and Biochemistry University of California at Los Angeles Los Angeles California
Publication Date:
Sponsoring Org.:
OSTI Identifier:
Grant/Contract Number:  
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Journal of Biophotonics
Additional Journal Information:
Journal Name: Journal of Biophotonics Journal Volume: 12 Journal Issue: 3; Journal ID: ISSN 1864-063X
Wiley Blackwell (John Wiley & Sons)
Country of Publication:

Citation Formats

Chen, Sez‐Jade, Sinsuebphon, Nattawut, Rudkouskaya, Alena, Barroso, Margarida, Intes, Xavier, and Michalet, Xavier. In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short‐lifetime near‐infrared dyes and time‐gated imaging. Germany: N. p., 2018. Web. doi:10.1002/jbio.201800185.
Chen, Sez‐Jade, Sinsuebphon, Nattawut, Rudkouskaya, Alena, Barroso, Margarida, Intes, Xavier, & Michalet, Xavier. In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short‐lifetime near‐infrared dyes and time‐gated imaging. Germany.
Chen, Sez‐Jade, Sinsuebphon, Nattawut, Rudkouskaya, Alena, Barroso, Margarida, Intes, Xavier, and Michalet, Xavier. Sun . "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short‐lifetime near‐infrared dyes and time‐gated imaging". Germany.
title = {In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short‐lifetime near‐infrared dyes and time‐gated imaging},
author = {Chen, Sez‐Jade and Sinsuebphon, Nattawut and Rudkouskaya, Alena and Barroso, Margarida and Intes, Xavier and Michalet, Xavier},
abstractNote = {},
doi = {10.1002/jbio.201800185},
journal = {Journal of Biophotonics},
number = 3,
volume = 12,
place = {Germany},
year = {2018},
month = {12}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 12 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Time-resolved fluorescence microscopy
journal, January 2005

  • Suhling, Klaus; French, Paul M. W.; Phillips, David
  • Photochemical & Photobiological Sciences, Vol. 4, Issue 1
  • DOI: 10.1039/b412924p

Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale
journal, December 1993

Full-field time-resolved fluorescence tomography of small animals
journal, January 2010

  • Venugopal, Vivek; Chen, Jin; Lesage, Frederic
  • Optics Letters, Vol. 35, Issue 19
  • DOI: 10.1364/OL.35.003189

Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures
journal, March 2017

  • Hato, Takashi; Winfree, Seth; Day, Richard
  • Journal of the American Society of Nephrology, Vol. 28, Issue 8
  • DOI: 10.1681/ASN.2016101153

Phasor imaging with a widefield photon-counting detector
journal, January 2012

  • Colyer, Ryan A.; Siegmund, Oswald H. W.; Tremsin, Anton S.
  • Journal of Biomedical Optics, Vol. 17, Issue 1
  • DOI: 10.1117/1.JBO.17.1.016008

Non fitting based FRET–FLIM analysis approaches applied to quantify protein–protein interactions in live cells
journal, May 2011

  • Padilla-Parra, Sergi; Auduge, Nicolas; Coppey-Moisan, Maite
  • Biophysical Reviews, Vol. 3, Issue 2
  • DOI: 10.1007/s12551-011-0047-6

Investigations of protein–protein interactions using time-resolved fluorescence and phasors
journal, March 2013

Quantitative tomographic imaging of intermolecular FRET in small animals
journal, January 2012

  • Venugopal, Vivek; Chen, Jin; Barroso, Margarida
  • Biomedical Optics Express, Vol. 3, Issue 12
  • DOI: 10.1364/BOE.3.003161

Mesoscopic Fluorescence Tomography of a Photosensitizer (HPPH) 3D Biodistribution in Skin Cancer
journal, February 2014

Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging
journal, January 2017

  • Cutrale, Francesco; Trivedi, Vikas; Trinh, Le A.
  • Nature Methods, Vol. 14, Issue 2
  • DOI: 10.1038/nmeth.4134

Receptor Complexes Cotransported via Polarized Endocytic Pathways Form Clusters with Distinct Organizations
journal, June 2007

  • Wallrabe, H.; Bonamy, G.; Periasamy, A.
  • Molecular Biology of the Cell, Vol. 18, Issue 6
  • DOI: 10.1091/mbc.E06-08-0700

FRET imaging
journal, October 2003

  • Jares-Erijman, Elizabeth A.; Jovin, Thomas M.
  • Nature Biotechnology, Vol. 21, Issue 11
  • DOI: 10.1038/nbt896

Non-Euclidean phasor analysis for quantification of oxidative stress in ex vivo human skin exposed to sun filters using fluorescence lifetime imaging microscopy
journal, December 2017

  • Osseiran, Sam; Roider, Elisabeth M.; Wang, Hequn
  • Journal of Biomedical Optics, Vol. 22, Issue 12
  • DOI: 10.1117/1.JBO.22.12.125004

The transferrin receptor part I: Biology and targeting with cytotoxic antibodies for the treatment of cancer
journal, November 2006

μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure
journal, December 2017

In vivo near-infrared fluorescence imaging
journal, October 2003

The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
journal, January 1984

  • Jameson, David M.; Gratton, Enrico; Hall, Robert D.
  • Applied Spectroscopy Reviews, Vol. 20, Issue 1
  • DOI: 10.1080/05704928408081716

Mesoscopic Fluorescence Molecular Tomography for Evaluating Engineered Tissues
journal, December 2015

  • Ozturk, Mehmet S.; Chen, Chao-Wei; Ji, Robin
  • Annals of Biomedical Engineering, Vol. 44, Issue 3
  • DOI: 10.1007/s10439-015-1511-4

Sensitivity and specificity of PET/CT regarding the detection of lymph node metastases in prostate cancer recurrence
journal, July 2014

The EPR effect: Unique features of tumor blood vessels for drug delivery, factors involved, and limitations and augmentation of the effect
journal, March 2011

The transferrin receptor and the targeted delivery of therapeutic agents against cancer
journal, March 2012

  • Daniels, Tracy R.; Bernabeu, Ezequiel; Rodríguez, José A.
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1820, Issue 3
  • DOI: 10.1016/j.bbagen.2011.07.016

The Phasor Approach to Fluorescence Lifetime Imaging Analysis
journal, January 2008

Phasor analysis of multiphoton spectral images distinguishes autofluorescence components of in vivo human skin : Phasor analysis of skin images
journal, April 2013

  • Fereidouni, Farzad; Bader, Arjen N.; Colonna, Anne
  • Journal of Biophotonics, Vol. 7, Issue 8
  • DOI: 10.1002/jbio.201200244

Molecular imaging in drug development
journal, July 2008

  • Willmann, Jürgen K.; van Bruggen, Nicholas; Dinkelborg, Ludger M.
  • Nature Reviews Drug Discovery, Vol. 7, Issue 7
  • DOI: 10.1038/nrd2290

Predicting in vivo fluorescence lifetime behavior of near-infrared fluorescent contrast agents using in vitro measurements
journal, January 2008

  • Akers, Walter J.; Berezin, Mikhail Y.; Lee, Hyeran
  • Journal of Biomedical Optics, Vol. 13, Issue 5
  • DOI: 10.1117/1.2982535

A novel fluorescence lifetime imaging system that optimizes photon efficiency
journal, January 2008

  • Colyer, Ryan A.; Lee, Claudia; Gratton, Enrico
  • Microscopy Research and Technique, Vol. 71, Issue 3
  • DOI: 10.1002/jemt.20540

Fluorescence-lifetime determination in tissues or other scattering media from measurement of excitation and emission kinetics
journal, January 1996

  • Hutchinson, Christina L.; Troy, Tamara L.; Sevick-Muraca, Eva M.
  • Applied Optics, Vol. 35, Issue 13
  • DOI: 10.1364/AO.35.002325

827Spatio-Temporal Quantification of FRET in Living Cells by Fast Time-Domain FLIM: A Comparative Study of Non-Fitting Methods
journal, July 2013

Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM
journal, December 2012

  • Hinde, E.; Digman, M. A.; Hahn, K. M.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 1
  • DOI: 10.1073/pnas.1211882110

Water in Biomolecular Fluorescence Spectroscopy and Imaging: Side Effects and Remedies
journal, February 2017

  • Fürstenberg, Alexandre
  • CHIMIA International Journal for Chemistry, Vol. 71, Issue 1
  • DOI: 10.2533/chimia.2017.26

The effects of self-absorption and detection geometry on fluorescence intensity and decay lifetime
conference, March 2005

  • Cormier, Jean-Francois; Fortin, Michel; Frechette, Julie
  • Biomedical Optics 2005, SPIE Proceedings
  • DOI: 10.1117/12.591026

Improved Super-Resolution Microscopy with Oxazine Fluorophores in Heavy Water
journal, July 2013

  • Lee, Steven F.; Vérolet, Quentin; Fürstenberg, Alexandre
  • Angewandte Chemie International Edition, Vol. 52, Issue 34
  • DOI: 10.1002/anie.201302341

Spatial light modulator based active wide-field illumination for ex vivo and in vivo quantitative NIR FRET imaging
journal, January 2014

  • Zhao, Lingling; Abe, Ken; Rajoria, Shilpi
  • Biomedical Optics Express, Vol. 5, Issue 3
  • DOI: 10.1364/BOE.5.000944

Near-infrared fluorescence: application to in vivo molecular imaging
journal, February 2010

Development of an optical imaging platform for functional imaging of small animals using wide-field excitation
journal, January 2010

  • Venugopal, Vivek; Chen, Jin; Intes, Xavier
  • Biomedical Optics Express, Vol. 1, Issue 1
  • DOI: 10.1364/BOE.1.000143

Fluorescence Lifetime Techniques in Medical Applications
journal, January 2012

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study
journal, September 2017

  • Fereidouni, Farzad; Gorpas, Dimitris; Ma, Dinglong
  • Methods and Applications in Fluorescence, Vol. 5, Issue 3
  • DOI: 10.1088/2050-6120/aa7b62

A modified phasor approach for analyzing time-gated fluorescence lifetime images: TIME-GATED PHASOR APPROACH
journal, September 2011

Adriamycin Conjugates of Human Transferrin Bind Transferrin Receptors and Kill K562 and HL60-Cells
journal, January 1993

  • Berczi, A.; Barabas, K.; Sizensky, J. A.
  • Archives of Biochemistry and Biophysics, Vol. 300, Issue 1
  • DOI: 10.1006/abbi.1993.1048

Multi-component quantitative magnetic resonance imaging by phasor representation
journal, April 2017

Targeted Drug Delivery via the Transferrin Receptor-Mediated Endocytosis Pathway
journal, December 2002

Optical properties of biological tissues: a review
journal, May 2013

Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data
journal, January 2004

Application of phasor plot and autofluorescence correction for study of heterogeneous cell population
journal, April 2014

  • Szmacinski, Henryk; Toshchakov, Vladimir; Lakowicz, Joseph R.
  • Journal of Biomedical Optics, Vol. 19, Issue 4
  • DOI: 10.1117/1.JBO.19.4.046017

Receptor–ligand binding assays: Technologies and Applications
journal, December 2005

Resolution of the fluorescence lifetimes in a heterogeneous system by phase and modulation measurements
journal, April 1981

Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue
journal, August 2011

  • Stringari, C.; Cinquin, A.; Cinquin, O.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 33
  • DOI: 10.1073/pnas.1108161108

Assessment of Gate Width Size on Lifetime-Based Förster Resonance Energy Transfer Parameter Estimation
journal, September 2015

Contributions of fluorescence techniques to understanding G protein-coupled receptor dimerisation
journal, April 2012

To exploit the tumor microenvironment: Passive and active tumor targeting of nanocarriers for anti-cancer drug delivery
journal, December 2010

Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy
journal, August 2011

  • Hinde, Elizabeth; Digman, Michelle A.; Welch, Christopher
  • Microscopy Research and Technique, Vol. 75, Issue 3
  • DOI: 10.1002/jemt.21054

Quantitative imaging of receptor-ligand engagement in intact live animals
journal, September 2018

Polar Plot Representation for Frequency-Domain Analysis of Fluorescence Lifetimes
journal, September 2005

Active wide-field illumination for high-throughput fluorescence lifetime imaging
journal, January 2013

  • Zhao, Lingling; Abe, Ken; Barroso, Margarida
  • Optics Letters, Vol. 38, Issue 19
  • DOI: 10.1364/OL.38.003976

In Vivo Photoacoustic Tomography of Chemicals: High-Resolution Functional and Molecular Optical Imaging at New Depths
journal, May 2010

  • Kim, Chulhong; Favazza, Christopher; Wang, Lihong V.
  • Chemical Reviews, Vol. 110, Issue 5
  • DOI: 10.1021/cr900266s

A Molecular Imaging Primer: Modalities, Imaging Agents, and Applications
journal, April 2012

Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis
journal, January 2018

Applications of phasor plots to in vitro protein studies
journal, March 2011

  • James, Nicholas G.; Ross, Justin A.; Štefl, Martin
  • Analytical Biochemistry, Vol. 410, Issue 1
  • DOI: 10.1016/j.ab.2010.11.011