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Title: Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi

Abstract

Anaerobic fungi are among the most active plant-degrading microbes in nature. Increased insight into the mechanisms and environmental cues that regulate fungal hydrolysis would better inform bioprocessing strategies to depolymerize lignocellulose. In this work, we compare the response of three strains of anaerobic fungi (Piromyces finnis, Anaeromyces robustus, and Neocallimastix californiae) to catabolite regulation by simple carbohydrates. Anaerobic fungi exhibited high enzymatic activity against crystalline cellulose, which was repressed upon incubation with free sugars. Cellulolytic degradation was also inhibited when fungi were exposed to sugars they did not metabolize, suggesting a general mode of catabolite repression. RNA-Seq experiments in the presence of excess glucose confirmed repression of carbohydrate active enzymes during sugar uptake, and offer a path towards unmasking the function of co-regulated genes that could be involved in biomass degradation. Lastly, these results suggest that sugar-rich hydrolysates tune the behavior of anaerobic fungi by dampening production of their biomass-degrading enzymes.

Authors:
 [1];  [1];  [1];  [1]; ORCiD logo [1]
  1. Univ. of California, Santa Barbara, CA (United States). Dept. of Chemical Engineering
Publication Date:
Research Org.:
Univ. of California, Santa Barbara, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division; USDOE
OSTI Identifier:
1485151
Alternate Identifier(s):
OSTI ID: 1475228
Grant/Contract Number:  
SC0010352; AC02-05CH11231; AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
AIChE Journal
Additional Journal Information:
Journal Volume: 64; Journal Issue: 12; Journal ID: ISSN 0001-1541
Publisher:
American Institute of Chemical Engineers
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; anaerobic fungi; cellulases; biofuel; regulation; Neocallimastigales

Citation Formats

Henske, John K., Gilmore, Sean P., Haitjema, Charles H., Solomon, Kevin V., and O'Malley, Michelle A. Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi. United States: N. p., 2018. Web. doi:10.1002/aic.16395.
Henske, John K., Gilmore, Sean P., Haitjema, Charles H., Solomon, Kevin V., & O'Malley, Michelle A. Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi. United States. doi:10.1002/aic.16395.
Henske, John K., Gilmore, Sean P., Haitjema, Charles H., Solomon, Kevin V., and O'Malley, Michelle A. Thu . "Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi". United States. doi:10.1002/aic.16395. https://www.osti.gov/servlets/purl/1485151.
@article{osti_1485151,
title = {Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi},
author = {Henske, John K. and Gilmore, Sean P. and Haitjema, Charles H. and Solomon, Kevin V. and O'Malley, Michelle A.},
abstractNote = {Anaerobic fungi are among the most active plant-degrading microbes in nature. Increased insight into the mechanisms and environmental cues that regulate fungal hydrolysis would better inform bioprocessing strategies to depolymerize lignocellulose. In this work, we compare the response of three strains of anaerobic fungi (Piromyces finnis, Anaeromyces robustus, and Neocallimastix californiae) to catabolite regulation by simple carbohydrates. Anaerobic fungi exhibited high enzymatic activity against crystalline cellulose, which was repressed upon incubation with free sugars. Cellulolytic degradation was also inhibited when fungi were exposed to sugars they did not metabolize, suggesting a general mode of catabolite repression. RNA-Seq experiments in the presence of excess glucose confirmed repression of carbohydrate active enzymes during sugar uptake, and offer a path towards unmasking the function of co-regulated genes that could be involved in biomass degradation. Lastly, these results suggest that sugar-rich hydrolysates tune the behavior of anaerobic fungi by dampening production of their biomass-degrading enzymes.},
doi = {10.1002/aic.16395},
journal = {AIChE Journal},
number = 12,
volume = 64,
place = {United States},
year = {2018},
month = {8}
}

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