Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi

Henske, John K.; Gilmore, Sean P.; Haitjema, Charles H.; Solomon, Kevin V.; O'Malley, Michelle A.

doi:10.1002/aic.16395

Title: Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi

Journal Article · 30 August 2018 · AIChE Journal

DOI: https://doi.org/10.1002/aic.16395 · OSTI ID:1485151

Henske, John K. ^[1]; Gilmore, Sean P. ^[2]; Haitjema, Charles H. ^[2]; Solomon, Kevin V. ^[2];

^[2]

Univ. of California, Santa Barbara, CA (United States). Dept. of Chemical Engineering; UCSB
Univ. of California, Santa Barbara, CA (United States). Dept. of Chemical Engineering

Anaerobic fungi are among the most active plant-degrading microbes in nature. Increased insight into the mechanisms and environmental cues that regulate fungal hydrolysis would better inform bioprocessing strategies to depolymerize lignocellulose. In this work, we compare the response of three strains of anaerobic fungi (Piromyces finnis, Anaeromyces robustus, and Neocallimastix californiae) to catabolite regulation by simple carbohydrates. Anaerobic fungi exhibited high enzymatic activity against crystalline cellulose, which was repressed upon incubation with free sugars. Cellulolytic degradation was also inhibited when fungi were exposed to sugars they did not metabolize, suggesting a general mode of catabolite repression. RNA-Seq experiments in the presence of excess glucose confirmed repression of carbohydrate active enzymes during sugar uptake, and offer a path towards unmasking the function of co-regulated genes that could be involved in biomass degradation. Lastly, these results suggest that sugar-rich hydrolysates tune the behavior of anaerobic fungi by dampening production of their biomass-degrading enzymes.

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Research Organization:: Univ. of California, Santa Barbara, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division

Grant/Contract Number:: SC0010352; AC02-05CH11231; AC05-76RL01830

OSTI ID:: 1485151

Journal Information:: AIChE Journal, Journal Name: AIChE Journal Journal Issue: 12 Vol. 64; ISSN 0001-1541

Publisher:: American Institute of Chemical EngineersCopyright Statement

Country of Publication:: United States

Language:: English

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