pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose
Abstract
Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.
- Authors:
-
- University of Illinois at Urbana-Champaign, IL (United States)
- Publication Date:
- Research Org.:
- Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1991853
- Alternate Identifier(s):
- OSTI ID: 1484862
- Grant/Contract Number:
- SC0018420
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biotechnology
- Additional Journal Information:
- Journal Volume: 290; Journal ID: ISSN 0168-1656
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Yarrowia lipolytica; Citric acid; Oleaginous yeast; Lipids; Transporter; RNAseq; citric acid; oleaginous yeast; lipids; transporter
Citation Formats
Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, and Rao, Christopher V. pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose. United States: N. p., 2018.
Web. doi:10.1016/j.jbiotec.2018.10.012.
Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, & Rao, Christopher V. pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose. United States. https://doi.org/10.1016/j.jbiotec.2018.10.012
Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, and Rao, Christopher V. Mon .
"pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose". United States. https://doi.org/10.1016/j.jbiotec.2018.10.012. https://www.osti.gov/servlets/purl/1991853.
@article{osti_1991853,
title = {pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose},
author = {Zhang, Shuyan and Jagtap, Sujit Sadashiv and Deewan, Anshu and Rao, Christopher V.},
abstractNote = {Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.},
doi = {10.1016/j.jbiotec.2018.10.012},
journal = {Journal of Biotechnology},
number = ,
volume = 290,
place = {United States},
year = {Mon Nov 26 00:00:00 EST 2018},
month = {Mon Nov 26 00:00:00 EST 2018}
}
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Works referencing / citing this record:
Corrigendum: Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica
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