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Title: Viscous control of cellular respiration by membrane lipid composition

Abstract

Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coli allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.


Citation Formats

Budin, Itay, de Rond, Tristan, Chen, Yan, Chan, Leanne Jade G., Petzold, Christopher J., and Keasling, Jay D. Viscous control of cellular respiration by membrane lipid composition. United States: N. p., 2018. Web. doi:10.1126/science.aat7925.
Budin, Itay, de Rond, Tristan, Chen, Yan, Chan, Leanne Jade G., Petzold, Christopher J., & Keasling, Jay D. Viscous control of cellular respiration by membrane lipid composition. United States. doi:10.1126/science.aat7925.
Budin, Itay, de Rond, Tristan, Chen, Yan, Chan, Leanne Jade G., Petzold, Christopher J., and Keasling, Jay D. Thu . "Viscous control of cellular respiration by membrane lipid composition". United States. doi:10.1126/science.aat7925.
@article{osti_1484838,
title = {Viscous control of cellular respiration by membrane lipid composition},
author = {Budin, Itay and de Rond, Tristan and Chen, Yan and Chan, Leanne Jade G. and Petzold, Christopher J. and Keasling, Jay D.},
abstractNote = {Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coli allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.},
doi = {10.1126/science.aat7925},
journal = {Science},
number = 6419,
volume = 362,
place = {United States},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aat7925

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Cited by: 9 works
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Works referenced in this record:

Tuning genetic control through promoter engineering
journal, August 2005

  • Alper, H.; Fischer, C.; Nevoigt, E.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 36, p. 12678-12683
  • DOI: 10.1073/pnas.0504604102