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Title: Bioconversion of Pelletized Big Bluestem, Switchgrass, and Low-Diversity Grass Mixtures Into Sugars and Bioethanol

Abstract

Three crops of warm-season grasses are being developed for biomass production on northern rain-fed marginal farmland: big bluestem (BBS), switchgrass (SG), and a low diversity mixture of grasses (LDM). In this study, biomass harvested from established fields were compared for pelletization and subsequent conversion to sugars and ethanol. Each biomass was successfully pelletized to similar bulk densities without adding a binder at a commercial feed operation. Pelletizing increased the bulk density by 407% on average and was equally effective on all three biomass samples (528–554 kg/m 3). Chemical analysis of the samples indicated that glucan and xylan contents were slightly reduced during pelletizing (by 23 and 16 g/kg, respectively), as well as theoretical ethanol yields, which are based upon total carbohydrate contents. Pellets and milled straws were pre-treated with either liquid hot-water or low-moisture ammonium hydroxide (LMA) and subsequently hydrolyzed with cellulases. Glucose and total sugar yields were similar for non-pellets and pellets using either pre-treatment; carbohydrates present in pellets were more efficiently recovered compared to non-pellets. LMA pretreated samples were separately hydrolyzed and fermented to ethanol using Scheffersomyces stipitis yeast. Hydrolysis recovered 69.7–76.8% of the glucose and 66.5–73.3% of the xylose across all samples. Glucose yields were 251–279 g/kg,more » db and were significantly lower for SG as compared to the other biomass samples. Recovered sugars were fermented to ethanol at 77.7–86.7% of theoretical yield. As a result, final ethanol yields (245.9–275.5 L/Mg, db) were similar for all of the grasses and estimated to equate to production levels for BBS, LDM, and SG of 1,952, 2,586, and 2,636 l of ethanol per ha, respectively.« less

Authors:
 [1];  [2];  [3];  [4];  [3];  [4];  [5];  [1]
  1. USDA-ARS, Peoria, IL (United States); Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States)
  2. Univ. of Nebraska, Lincoln, NE (United States)
  3. USDA-ARS, Peoria, IL (United States)
  4. Univ. of Nebraska-East, Lincoln, NE (United States)
  5. Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States)
Publication Date:
Research Org.:
Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States). Center for Advanced Bioenergy and Bioproducts Innovation (CABBI)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1484754
Grant/Contract Number:  
SC0018420
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Energy Research
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2296-598X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bioethanol; bioenergy crops; pellets; sugars; grasses

Citation Formats

Dien, Bruce S., Mitchell, Robert B., Bowman, Michael J., Jin, Virginia L., Quarterman, Joshua, Schmer, Marty R., Singh, Vijay, and Slininger, Patricia J. Bioconversion of Pelletized Big Bluestem, Switchgrass, and Low-Diversity Grass Mixtures Into Sugars and Bioethanol. United States: N. p., 2018. Web. doi:10.3389/fenrg.2018.00129.
Dien, Bruce S., Mitchell, Robert B., Bowman, Michael J., Jin, Virginia L., Quarterman, Joshua, Schmer, Marty R., Singh, Vijay, & Slininger, Patricia J. Bioconversion of Pelletized Big Bluestem, Switchgrass, and Low-Diversity Grass Mixtures Into Sugars and Bioethanol. United States. doi:10.3389/fenrg.2018.00129.
Dien, Bruce S., Mitchell, Robert B., Bowman, Michael J., Jin, Virginia L., Quarterman, Joshua, Schmer, Marty R., Singh, Vijay, and Slininger, Patricia J. Tue . "Bioconversion of Pelletized Big Bluestem, Switchgrass, and Low-Diversity Grass Mixtures Into Sugars and Bioethanol". United States. doi:10.3389/fenrg.2018.00129. https://www.osti.gov/servlets/purl/1484754.
@article{osti_1484754,
title = {Bioconversion of Pelletized Big Bluestem, Switchgrass, and Low-Diversity Grass Mixtures Into Sugars and Bioethanol},
author = {Dien, Bruce S. and Mitchell, Robert B. and Bowman, Michael J. and Jin, Virginia L. and Quarterman, Joshua and Schmer, Marty R. and Singh, Vijay and Slininger, Patricia J.},
abstractNote = {Three crops of warm-season grasses are being developed for biomass production on northern rain-fed marginal farmland: big bluestem (BBS), switchgrass (SG), and a low diversity mixture of grasses (LDM). In this study, biomass harvested from established fields were compared for pelletization and subsequent conversion to sugars and ethanol. Each biomass was successfully pelletized to similar bulk densities without adding a binder at a commercial feed operation. Pelletizing increased the bulk density by 407% on average and was equally effective on all three biomass samples (528–554 kg/m3). Chemical analysis of the samples indicated that glucan and xylan contents were slightly reduced during pelletizing (by 23 and 16 g/kg, respectively), as well as theoretical ethanol yields, which are based upon total carbohydrate contents. Pellets and milled straws were pre-treated with either liquid hot-water or low-moisture ammonium hydroxide (LMA) and subsequently hydrolyzed with cellulases. Glucose and total sugar yields were similar for non-pellets and pellets using either pre-treatment; carbohydrates present in pellets were more efficiently recovered compared to non-pellets. LMA pretreated samples were separately hydrolyzed and fermented to ethanol using Scheffersomyces stipitis yeast. Hydrolysis recovered 69.7–76.8% of the glucose and 66.5–73.3% of the xylose across all samples. Glucose yields were 251–279 g/kg, db and were significantly lower for SG as compared to the other biomass samples. Recovered sugars were fermented to ethanol at 77.7–86.7% of theoretical yield. As a result, final ethanol yields (245.9–275.5 L/Mg, db) were similar for all of the grasses and estimated to equate to production levels for BBS, LDM, and SG of 1,952, 2,586, and 2,636 l of ethanol per ha, respectively.},
doi = {10.3389/fenrg.2018.00129},
journal = {Frontiers in Energy Research},
number = ,
volume = 6,
place = {United States},
year = {2018},
month = {12}
}

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Works referenced in this record:

Effect of process parameters on pelletisation of herbaceous crops
journal, August 2009


Conversion of switchgrass to ethanol using dilute ammonium hydroxide pretreatment: influence of ecotype and harvest maturity
journal, July 2013


Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
journal, February 2018

  • Biswal, Ajaya K.; Atmodjo, Melani A.; Li, Mi
  • Nature Biotechnology, Vol. 36, Issue 3
  • DOI: 10.1038/nbt.4067

Combined effect of pelleting and pretreatment on enzymatic hydrolysis of switchgrass
journal, July 2012


Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading
journal, April 2015

  • Slininger, Patricia J.; Shea-Andersh, Maureen A.; Thompson, Stephanie R.
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0239-6

Effect of pelleting process variables on physical properties and sugar yields of ammonia fiber expansion pretreated corn stover
journal, July 2014


Soil Carbon Storage by Switchgrass Grown for Bioenergy
journal, September 2008


Impact of Harvest Time and Switchgrass Cultivar on Sugar Release Through Enzymatic Hydrolysis
journal, November 2016

  • Serapiglia, Michelle J.; Dien, Bruce S.; Boateng, Akwasi A.
  • BioEnergy Research, Vol. 10, Issue 2
  • DOI: 10.1007/s12155-016-9803-6

Dedicated Herbaceous Biomass Feedstock Genetics and Development
journal, June 2016


Effects of the pelleting conditions on chemical composition and sugar yield of corn stover, big bluestem, wheat straw, and sorghum stalk pellets
journal, October 2011

  • Theerarattananoon, Karnnalin; Xu, Feng; Wilson, Jonathan
  • Bioprocess and Biosystems Engineering, Vol. 35, Issue 4, p. 615-623
  • DOI: 10.1007/s00449-011-0642-8

Cellulosic ethanol: status and innovation
journal, June 2017


Management of warm-season grass mixtures for biomass production in South Dakota USA
journal, February 2008


Big bluestem as a bioenergy crop: A review
journal, December 2015

  • Zhang, Ke; Johnson, Loretta; Vara Prasad, P. V.
  • Renewable and Sustainable Energy Reviews, Vol. 52
  • DOI: 10.1016/j.rser.2015.07.144

Senescence, dormancy and tillering in perennial C4 grasses
journal, March 2014


Pretreatment of corn stover using low-moisture anhydrous ammonia (LMAA) process
journal, November 2011


Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals
journal, January 2016

  • Nghiem, Nhuan P.; Senske, Gerard E.; Kim, Tae Hyun
  • Applied Biochemistry and Biotechnology, Vol. 179, Issue 1
  • DOI: 10.1007/s12010-016-1982-2

Cellulosic biofuel contributions to a sustainable energy future: Choices and outcomes
journal, June 2017

  • Robertson, G. Philip; Hamilton, Stephen K.; Barham, Bradford L.
  • Science, Vol. 356, Issue 6345
  • DOI: 10.1126/science.aal2324

The Effect of Biomass Densification on Structural Sugar Release and Yield in Biofuel Feedstock and Feedstock Blends
journal, January 2017


Switchgrass for forage and bioenergy: harvest and nitrogen rate effects on biomass yields and nutrient composition
journal, April 2010


Large-scale production, harvest and logistics of switchgrass ( Panicum virgatum L. ) - current technology and envisioning a mature technology
journal, March 2009

  • Sokhansanj, Shahab; Mani, Sudhagar; Turhollow, Anthony
  • Biofuels, Bioproducts and Biorefining, Vol. 3, Issue 2
  • DOI: 10.1002/bbb.129

Net energy of cellulosic ethanol from switchgrass
journal, January 2008

  • Schmer, M. R.; Vogel, K. P.; Mitchell, R. B.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 2
  • DOI: 10.1073/pnas.0704767105

Switchgrass Biomass Production in the Midwest USA
journal, January 2002

  • Vogel, Kenneth P.; Brejda, John J.; Walters, Daniel T.
  • Agronomy Journal, Vol. 94, Issue 3
  • DOI: 10.2134/agronj2002.0413

Accelerating the Switchgrass (Panicum virgatum L.) Breeding Cycle Using Genomic Selection Approaches
journal, November 2014


Reduced pretreatment severity and enzyme loading enabled through switchgrass pelleting
journal, August 2014


Perennial warm-season grasses for producing biofuel and enhancing soil properties: an alternative to corn residue removal
journal, March 2017

  • Blanco-Canqui, Humberto; Mitchell, Robert B.; Jin, Virginia L.
  • GCB Bioenergy, Vol. 9, Issue 9
  • DOI: 10.1111/gcbb.12436

Quantifying Actual and Theoretical Ethanol Yields for Switchgrass Strains Using NIRS Analyses
journal, August 2010


Impact of mixed feedstocks and feedstock densification on ionic liquid pretreatment efficiency
journal, January 2013

  • Shi, Jian; Thompson, Vicki S.; Yancey, Neal A.
  • Biofuels, Vol. 4, Issue 1
  • DOI: 10.4155/bfs.12.82

Midwest vision for sustainable fuel production
journal, November 2014


Opportunities and roadblocks in utilizing forages and small grains for liquid fuels
journal, January 2008

  • Sarath, Gautam; Mitchell, Robert B.; Sattler, Scott E.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 35, Issue 5
  • DOI: 10.1007/s10295-007-0296-3

Registration of ‘Liberty’ Switchgrass
journal, January 2014


Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers’ grains and their conversion to ethanol
journal, August 2008


A review of biomass densification systems to develop uniform feedstock commodities for bioenergy application
journal, October 2011

  • Tumuluru, Jaya Shankar; Wright, Christopher T.; Hess, J. Richard
  • Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 6
  • DOI: 10.1002/bbb.324

Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass
journal, October 2006


Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States
journal, June 2005


Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


The updated billion-ton resource assessment
journal, November 2014


Enzymatic hydrolysis of pelletized AFEX™-treated corn stover at high solid loadings: Hydrolysis of Pelletized AFEX Corn Stover
journal, September 2013

  • Bals, Bryan D.; Gunawan, Christa; Moore, Janette
  • Biotechnology and Bioengineering, Vol. 111, Issue 2
  • DOI: 10.1002/bit.25022

Long-term productivity of lowland and upland switchgrass cytotypes as affected by cutting frequency
journal, November 2008


Pseudo-lignin and pretreatment chemistry
journal, January 2011

  • Sannigrahi, Poulomi; Kim, Dong Ho; Jung, Seokwon
  • Energy Environ. Sci., Vol. 4, Issue 4
  • DOI: 10.1039/C0EE00378F

Compositional Analysis of Lignocellulosic Feedstocks. 2. Method Uncertainties
journal, August 2010

  • Templeton, David W.; Scarlata, Christopher J.; Sluiter, Justin B.
  • Journal of Agricultural and Food Chemistry, Vol. 58, Issue 16
  • DOI: 10.1021/jf100807b

Optimization of pH controlled liquid hot water pretreatment of corn stover
journal, December 2005


A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass
journal, January 2016


Comparative study on enzymatic digestibility of switchgrass varieties and harvests processed by leading pretreatment technologies
journal, December 2011


Natural binders and solid bridge type binding mechanisms in briquettes and pellets made from corn stover and switchgrass
journal, February 2010


Harvest Management of Switchgrass for Biomass Feedstock and Forage Production
journal, January 1999


Carbon-Negative Biofuels from Low-Input High-Diversity Grassland Biomass
journal, December 2006


Unique aspects of the grass cell wall
journal, June 2008


Techno-economic analysis of decentralized biomass processing depots
journal, October 2015