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Title: Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli

Abstract

Ureolytic bacteria (e.g., Sporosarcina pasteurii) can produce calcium carbonate (CaCO 3). Tailoring the size and shape of biogenic CaCO 3 may increase the range of useful applications for these crystals. However, wild type Sporosarcina pasteurii is difficult to genetically engineer, limiting control of the organism and its crystal precipitates. Therefore, we designed, constructed, and compared different urease operons and expression levels for CaCO 3 production in engineered Escherichia coli strains. We quantified urease expression and calcium uptake and characterized CaCO 3 crystal phase and morphology for 13 engineered strains. There was a weak relationship between urease expression and crystal size, suggesting that genes surrounding the urease gene cluster affect crystal size. However, when evaluating strains with a wider range of urease expression levels, there was a negative relationship between urease activity and polycrystal size (e.g., larger crystals with lower activity). The resulting range of crystal morphologies created by the rationally designed strains demonstrates the potential for controlling biogenic CaCO 3 precipitation.

Authors:
; ; ; ORCiD logo; ;  [1]; ; ; ORCiD logo
  1. National Renewable Energy Laboratory, Golden, Colorado 80401, United States
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
U.S. Department of Defense (DOD), Defense Advanced Research Projects Agency
OSTI Identifier:
1484428
Report Number(s):
NREL/JA-2700-72870
Journal ID: ISSN 2161-5063
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
ACS Synthetic Biology
Additional Journal Information:
Journal Volume: 7; Journal Issue: 11; Journal ID: ISSN 2161-5063
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CaCO3 precipitation; Escherichia coli; MICP; rational control; urease

Citation Formats

Liang, Liya, Heveran, Chelsea, Liu, Rongming, Gill, Ryan T., Nagarajan, Aparna, Cameron, Jeffrey, Hubler, Mija, Srubar, Wil V., and Cook, Sherri M. Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli. United States: N. p., 2018. Web. doi:10.1021/acssynbio.8b00194.
Liang, Liya, Heveran, Chelsea, Liu, Rongming, Gill, Ryan T., Nagarajan, Aparna, Cameron, Jeffrey, Hubler, Mija, Srubar, Wil V., & Cook, Sherri M. Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli. United States. doi:10.1021/acssynbio.8b00194.
Liang, Liya, Heveran, Chelsea, Liu, Rongming, Gill, Ryan T., Nagarajan, Aparna, Cameron, Jeffrey, Hubler, Mija, Srubar, Wil V., and Cook, Sherri M. Fri . "Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli". United States. doi:10.1021/acssynbio.8b00194. https://www.osti.gov/servlets/purl/1484428.
@article{osti_1484428,
title = {Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli},
author = {Liang, Liya and Heveran, Chelsea and Liu, Rongming and Gill, Ryan T. and Nagarajan, Aparna and Cameron, Jeffrey and Hubler, Mija and Srubar, Wil V. and Cook, Sherri M.},
abstractNote = {Ureolytic bacteria (e.g., Sporosarcina pasteurii) can produce calcium carbonate (CaCO3). Tailoring the size and shape of biogenic CaCO3 may increase the range of useful applications for these crystals. However, wild type Sporosarcina pasteurii is difficult to genetically engineer, limiting control of the organism and its crystal precipitates. Therefore, we designed, constructed, and compared different urease operons and expression levels for CaCO3 production in engineered Escherichia coli strains. We quantified urease expression and calcium uptake and characterized CaCO3 crystal phase and morphology for 13 engineered strains. There was a weak relationship between urease expression and crystal size, suggesting that genes surrounding the urease gene cluster affect crystal size. However, when evaluating strains with a wider range of urease expression levels, there was a negative relationship between urease activity and polycrystal size (e.g., larger crystals with lower activity). The resulting range of crystal morphologies created by the rationally designed strains demonstrates the potential for controlling biogenic CaCO3 precipitation.},
doi = {10.1021/acssynbio.8b00194},
journal = {ACS Synthetic Biology},
number = 11,
volume = 7,
place = {United States},
year = {2018},
month = {11}
}

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