Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli
Abstract
Ureolytic bacteria (e.g., Sporosarcina pasteurii) can produce calcium carbonate (CaCO3). Tailoring the size and shape of biogenic CaCO3 may increase the range of useful applications for these crystals. However, wild type Sporosarcina pasteurii is difficult to genetically engineer, limiting control of the organism and its crystal precipitates. Therefore, we designed, constructed, and compared different urease operons and expression levels for CaCO3 production in engineered Escherichia coli strains. We quantified urease expression and calcium uptake and characterized CaCO3 crystal phase and morphology for 13 engineered strains. There was a weak relationship between urease expression and crystal size, suggesting that genes surrounding the urease gene cluster affect crystal size. However, when evaluating strains with a wider range of urease expression levels, there was a negative relationship between urease activity and polycrystal size (e.g., larger crystals with lower activity). The resulting range of crystal morphologies created by the rationally designed strains demonstrates the potential for controlling biogenic CaCO3 precipitation.
- Authors:
-
[3];
[2]
- Renewable and Sustainable Energy Inst. (RASEI), Boulder, CO (United States)
- Univ. of Colorado, Boulder, CO (United States). Dept. of Civil, Environmental, and Architectural Engineering
- Renewable and Sustainable Energy Inst. (RASEI), Boulder, CO (United States); Univ. of Colorado, Boulder, CO (United States). Dept. of Chemical and Biological Engineering
- Renewable and Sustainable Energy Inst. (RASEI), Boulder, CO (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States). Dept. of Chemistry and Biochemistry
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- U.S. Department of Defense (DOD), Defense Advanced Research Projects Agency
- OSTI Identifier:
- 1484428
- Report Number(s):
- NREL/JA-2700-72870
Journal ID: ISSN 2161-5063
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Synthetic Biology
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 11; Journal ID: ISSN 2161-5063
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; CaCO3 precipitation; Escherichia coli; MICP; rational control; urease
Citation Formats
Liang, Liya, Heveran, Chelsea, Liu, Rongming, Gill, Ryan T., Nagarajan, Aparna, Cameron, Jeffrey, Hubler, Mija, Srubar, Wil V., and Cook, Sherri M. Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli. United States: N. p., 2018.
Web. doi:10.1021/acssynbio.8b00194.
Liang, Liya, Heveran, Chelsea, Liu, Rongming, Gill, Ryan T., Nagarajan, Aparna, Cameron, Jeffrey, Hubler, Mija, Srubar, Wil V., & Cook, Sherri M. Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli. United States. https://doi.org/10.1021/acssynbio.8b00194
Liang, Liya, Heveran, Chelsea, Liu, Rongming, Gill, Ryan T., Nagarajan, Aparna, Cameron, Jeffrey, Hubler, Mija, Srubar, Wil V., and Cook, Sherri M. Fri .
"Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli". United States. https://doi.org/10.1021/acssynbio.8b00194. https://www.osti.gov/servlets/purl/1484428.
@article{osti_1484428,
title = {Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli},
author = {Liang, Liya and Heveran, Chelsea and Liu, Rongming and Gill, Ryan T. and Nagarajan, Aparna and Cameron, Jeffrey and Hubler, Mija and Srubar, Wil V. and Cook, Sherri M.},
abstractNote = {Ureolytic bacteria (e.g., Sporosarcina pasteurii) can produce calcium carbonate (CaCO3). Tailoring the size and shape of biogenic CaCO3 may increase the range of useful applications for these crystals. However, wild type Sporosarcina pasteurii is difficult to genetically engineer, limiting control of the organism and its crystal precipitates. Therefore, we designed, constructed, and compared different urease operons and expression levels for CaCO3 production in engineered Escherichia coli strains. We quantified urease expression and calcium uptake and characterized CaCO3 crystal phase and morphology for 13 engineered strains. There was a weak relationship between urease expression and crystal size, suggesting that genes surrounding the urease gene cluster affect crystal size. However, when evaluating strains with a wider range of urease expression levels, there was a negative relationship between urease activity and polycrystal size (e.g., larger crystals with lower activity). The resulting range of crystal morphologies created by the rationally designed strains demonstrates the potential for controlling biogenic CaCO3 precipitation.},
doi = {10.1021/acssynbio.8b00194},
journal = {ACS Synthetic Biology},
number = 11,
volume = 7,
place = {United States},
year = {Fri Nov 02 00:00:00 EDT 2018},
month = {Fri Nov 02 00:00:00 EDT 2018}
}
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Works referencing / citing this record:
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Engineered Ureolytic Microorganisms Can Tailor the Morphology and Nanomechanical Properties of Microbial-Precipitated Calcium Carbonate
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