The Local Phosphate Deficiency Response Activates Endoplasmic Reticulum Stress-Dependent Autophagy

Journal Article · 01 February 2019 · Plant Physiology (Bethesda)

DOI: https://doi.org/10.1104/pp.18.01379 · OSTI ID:1484329

Naumann, Christin  ^[1]; Müller, Jens ^[1]; Sakhonwasee, Siriwat ^[1]; Wieghaus, Annika ^[1]; Hause, Gerd ^[2]; Heisters, Marcus ^[1]; Bürstenbinder, Katharina  ^[1]; Abel, Steffen  ^[3]

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1. Leibniz Inst. for Plant Biochemistry, Halle (Germany)
2. Martin Luther Univ., Halle (Germany)
3. Univ. of California, Davis, CA (United States); Leibniz Inst. for Plant Biochemistry, Halle (Germany); Martin Luther Univ., Halle (Germany)

Inorganic phosphate (Pi) is often a limiting plant nutrient. In members of the Brassicaceae family, such as Arabidopsis (Arabidopsis thaliana), Pi deprivation reshapes root system architecture to favor topsoil foraging. It does so by inhibiting primary root extension and stimulating lateral root formation. Root growth inhibition from phosphate (Pi) deficiency is triggered by iron-stimulated, apoplastic reactive oxygen species generation and cell wall modifications, which impair cell-to-cell communication and meristem maintenance. These processes require LOW PHOSPHATE RESPONSE1 (LPR1), a cell wall-targeted ferroxidase, and PHOSPHATE DEFICIENCY RESPONSE2 (PDR2), the single endoplasmic reticulum (ER)-resident P5-type ATPase (AtP5A), which is thought to control LPR1 secretion or activity. Autophagy is a conserved process involving the vacuolar degradation of cellular components. While the function of autophagy is well established under nutrient starvation (C, N, or S), it remains to be explored under Pi deprivation. Because AtP5A/PDR2 likely functions in the ER stress response, we analyzed the effect of Pi limitation on autophagy. Our comparative study of mutants defective in the local Pi deficiency response, ER stress response, and autophagy demonstrated that ER stress-dependent autophagy is rapidly activated as part of the developmental root response to Pi limitation and requires the genetic PDR2-LPR1 module. We conclude that Pi-dependent activation of autophagy in the root apex is a consequence of local Pi sensing and the associated ER stress response, rather than a means for systemic recycling of the macronutrient.

• Research Organization: Univ. of California, Davis, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC); USDOE
• Grant/Contract Number: FG02-03ER15447; DE–FG0203ER15447
• OSTI ID: 1484329
• Alternate ID(s): OSTI ID: 1609429
• Journal Information: Plant Physiology (Bethesda), Vol. 179, Issue 2; ISSN 0032-0889
• Publisher: American Society of Plant BiologistsCopyright Statement
• Country of Publication: United States
• Language: English