Polarization induced contrast X-ray fluorescence at submicrometer resolution reveals nanometer apatite crystal orientations across entire tooth sections
Abstract
For biomedical research, successful imaging of calcified microstructures often relies on absorption differences between features, or on employing dies with selective affinity to areas of interest. When texture is concerned, e.g. for crystal orientation studies, polarization induced contrast is of particular interest. This requires sufficient interaction of the incoming radiation with the volume of interest in the sample to produce orientation-based contrast. Here we demonstrate polarization induced contrast at the calcium K-edge using submicron sized monochromatic synchrotron X-ray beams. We exploit the orientation dependent subtle absorption differences of hydroxyl-apatite crystals in teeth, with respect to the polarization field of the beam. Interaction occurs with the fully mineralized samples, such that differences in density do not contribute to the contrast. Our results show how polarization induced contrast X-ray fluorescence mapping at specific energies of the calcium K-edge reveals the micrometer and submicrometer crystal arrangements in human tooth tissues. This facilitates combining both high spatial resolution and large fields of view, achieved in relatively short acquisition times in reflection geometry. In enamel we observe the varying crystal orientations of the micron sized prisms exposed on our prepared surface. We easily reproduce crystal orientation maps, typically observed in polished thin sections. We evenmore »
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1484326
- Alternate Identifier(s):
- OSTI ID: 1497983
- Report Number(s):
- LLNL-JRNL-756138
Journal ID: ISSN 2156-7085
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Published Article
- Journal Name:
- Biomedical Optics Express
- Additional Journal Information:
- Journal Name: Biomedical Optics Express Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 2156-7085
- Publisher:
- Optical Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Hesse, Bernhard, Stier, Deborah, Cotte, Marine, Forien, Jean-Baptiste, and Zaslansky, Paul. Polarization induced contrast X-ray fluorescence at submicrometer resolution reveals nanometer apatite crystal orientations across entire tooth sections. United States: N. p., 2018.
Web. doi:10.1364/BOE.10.000018.
Hesse, Bernhard, Stier, Deborah, Cotte, Marine, Forien, Jean-Baptiste, & Zaslansky, Paul. Polarization induced contrast X-ray fluorescence at submicrometer resolution reveals nanometer apatite crystal orientations across entire tooth sections. United States. https://doi.org/10.1364/BOE.10.000018
Hesse, Bernhard, Stier, Deborah, Cotte, Marine, Forien, Jean-Baptiste, and Zaslansky, Paul. Mon .
"Polarization induced contrast X-ray fluorescence at submicrometer resolution reveals nanometer apatite crystal orientations across entire tooth sections". United States. https://doi.org/10.1364/BOE.10.000018.
@article{osti_1484326,
title = {Polarization induced contrast X-ray fluorescence at submicrometer resolution reveals nanometer apatite crystal orientations across entire tooth sections},
author = {Hesse, Bernhard and Stier, Deborah and Cotte, Marine and Forien, Jean-Baptiste and Zaslansky, Paul},
abstractNote = {For biomedical research, successful imaging of calcified microstructures often relies on absorption differences between features, or on employing dies with selective affinity to areas of interest. When texture is concerned, e.g. for crystal orientation studies, polarization induced contrast is of particular interest. This requires sufficient interaction of the incoming radiation with the volume of interest in the sample to produce orientation-based contrast. Here we demonstrate polarization induced contrast at the calcium K-edge using submicron sized monochromatic synchrotron X-ray beams. We exploit the orientation dependent subtle absorption differences of hydroxyl-apatite crystals in teeth, with respect to the polarization field of the beam. Interaction occurs with the fully mineralized samples, such that differences in density do not contribute to the contrast. Our results show how polarization induced contrast X-ray fluorescence mapping at specific energies of the calcium K-edge reveals the micrometer and submicrometer crystal arrangements in human tooth tissues. This facilitates combining both high spatial resolution and large fields of view, achieved in relatively short acquisition times in reflection geometry. In enamel we observe the varying crystal orientations of the micron sized prisms exposed on our prepared surface. We easily reproduce crystal orientation maps, typically observed in polished thin sections. We even reveal maps of submicrometer mineralization fronts in spherulites in intertubular dentine. This Ca K-edge polarization sensitive method (XRF-PIC) does not require thin samples for transmission nor extensive sample preparation. It can be used on both fresh, moist samples as well as fossilized samples where the information of interests lies in the crystal orientations and where the crystalline domains extend several micrometers beneath the exposed surface.},
doi = {10.1364/BOE.10.000018},
journal = {Biomedical Optics Express},
number = 1,
volume = 10,
place = {United States},
year = {Mon Dec 03 00:00:00 EST 2018},
month = {Mon Dec 03 00:00:00 EST 2018}
}
https://doi.org/10.1364/BOE.10.000018
Web of Science
Figures / Tables:
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Works referencing / citing this record:
The hidden structure of human enamel
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Figures / Tables found in this record: