Title: Computational multifocal microscopy

Abstract

Despite recent advances, high performance single-shot 3D microscopy remains an elusive task. By introducing designed diffractive optical elements (DOEs), one is capable of converting a microscope into a 3D "kaleidoscope", in which case the snapshot image consists of an array of tiles and each tile focuses on different depths. However, the acquired multifocal microscopic (MFM) image suffers from multiple sources of degradation, which prevents MFM from further applications. We propose a unifying computational framework which simplifies the imaging system and achieves 3D reconstruction via computation. Our optical configuration omits chromatic correction grating and redesigns the multifocal grating to enlarge the tracking area. Our proposed setup features only one single grating in addition to a regular microscope.The aberration correction, along with Poisson and background denoising, are incorporated in our deconvolution-based fully-automated algorithm, which requires no empirical parameter-tuning. In experiments, we achieve the spatial resolutions of 0:35um (lateral) and 0:5um (axial), which are comparable to the resolution that can be achieved with confocal deconvolution microscopy. We demonstrate a 3D video of moving bacteria recorded at 25 frames per second using our proposed computational multifocal microscopy technique.

Authors:

He, Kuan; Wang, Zihao ;

• Search DOE PAGES for author "Wang, Zihao"
• Search DOE PAGES for ORCID "0000-0003-4162-7849"

Huang, Xiang; Wang, Xiaolei; Yoo, Seunghwan; Ruiz, Pablo; Gdor, Itay ;

• Search DOE PAGES for author "Gdor, Itay"
• Search DOE PAGES for ORCID "0000-0002-5900-6082"

Selewa, Alan; Ferrier, Nicola J.; Scherer, Norbert; Hereld, Mark ;

• Search DOE PAGES for author "Hereld, Mark"
• Search DOE PAGES for ORCID "0000-0002-0268-2880"

Katsaggelos, Aggelos K.; Cossairt, Oliver

Publication Date:

Wed Nov 28 00:00:00 EST 2018

Research Org.:

Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

OSTI Identifier:

1483720

Alternate Identifier(s):

OSTI ID: 1493888

Grant/Contract Number:  

AC02-06CH11357

Resource Type:

Published Article

Journal Name:

Biomedical Optics Express

Additional Journal Information:

Journal Name: Biomedical Optics Express Journal Volume: 9 Journal Issue: 12; Journal ID: ISSN 2156-7085

Publisher:

Optical Society of America

Country of Publication:

United States

Language:

English

Subject:

36 MATERIALS SCIENCE; 97 MATHEMATICS AND COMPUTING