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Title: Drought increases the frequencies of fungal functional genes related to carbon and nitrogen acquisition

Abstract

Although water is a critical resource for organisms, microbially-mediated processes such as decomposition and nitrogen (N) transformations can endure within ecosystems even when water is scarce. To identify underlying mechanisms, we examined the genetic potential for fungi to contribute to specific aspects of carbon (C) and N cycling in a drought manipulation in Southern California grassland. In particular, we measured the frequency of fungal functional genes encoding enzymes that break down cellulose and chitin, and take up ammonium and amino acids, in decomposing litter. Furthermore, we used “microbial cages” to reciprocally transplant litter and microbes between control and drought plots. This approach allowed us to distinguish direct effects of drought in the plot environment versus indirect effects via shifts in the microbial community or changes in litter chemistry. For every fungal functional gene we examined, the frequency of that gene within the microbial community increased significantly in drought plots compared to control plots. In contrast, when plot environment was held constant, frequencies of these fungal functional genes did not differ significantly between control-derived microbes versus drought-derived microbes, or between control-derived litter versus drought-derived litter. It appears that drought directly selects for fungi with the genetic capacity to acquire these specificmore » C- and N-containing compounds. This genetic trait may allow fungi to take advantage of ephemeral water supplies. Altogether, proliferation of fungi with the genetic capacity for C and N acquisition may contribute to the maintenance of biogeochemical cycling under drought.« less

Authors:
ORCiD logo; ; ; ;
Publication Date:
Research Org.:
Univ. of California, Irvine, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
OSTI Identifier:
1483023
Alternate Identifier(s):
OSTI ID: 1612542
Grant/Contract Number:  
PS02-09ER09-25; SC001641; SC0016410; EAR-1411942; DEB-1457160
Resource Type:
Published Article
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Name: PLoS ONE Journal Volume: 13 Journal Issue: 11; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Treseder, Kathleen K., Berlemont, Renaud, Allison, Steven D., Martiny, Adam C., and Hu, ed., Shuijin. Drought increases the frequencies of fungal functional genes related to carbon and nitrogen acquisition. United States: N. p., 2018. Web. doi:10.1371/journal.pone.0206441.
Treseder, Kathleen K., Berlemont, Renaud, Allison, Steven D., Martiny, Adam C., & Hu, ed., Shuijin. Drought increases the frequencies of fungal functional genes related to carbon and nitrogen acquisition. United States. doi:10.1371/journal.pone.0206441.
Treseder, Kathleen K., Berlemont, Renaud, Allison, Steven D., Martiny, Adam C., and Hu, ed., Shuijin. Wed . "Drought increases the frequencies of fungal functional genes related to carbon and nitrogen acquisition". United States. doi:10.1371/journal.pone.0206441.
@article{osti_1483023,
title = {Drought increases the frequencies of fungal functional genes related to carbon and nitrogen acquisition},
author = {Treseder, Kathleen K. and Berlemont, Renaud and Allison, Steven D. and Martiny, Adam C. and Hu, ed., Shuijin},
abstractNote = {Although water is a critical resource for organisms, microbially-mediated processes such as decomposition and nitrogen (N) transformations can endure within ecosystems even when water is scarce. To identify underlying mechanisms, we examined the genetic potential for fungi to contribute to specific aspects of carbon (C) and N cycling in a drought manipulation in Southern California grassland. In particular, we measured the frequency of fungal functional genes encoding enzymes that break down cellulose and chitin, and take up ammonium and amino acids, in decomposing litter. Furthermore, we used “microbial cages” to reciprocally transplant litter and microbes between control and drought plots. This approach allowed us to distinguish direct effects of drought in the plot environment versus indirect effects via shifts in the microbial community or changes in litter chemistry. For every fungal functional gene we examined, the frequency of that gene within the microbial community increased significantly in drought plots compared to control plots. In contrast, when plot environment was held constant, frequencies of these fungal functional genes did not differ significantly between control-derived microbes versus drought-derived microbes, or between control-derived litter versus drought-derived litter. It appears that drought directly selects for fungi with the genetic capacity to acquire these specific C- and N-containing compounds. This genetic trait may allow fungi to take advantage of ephemeral water supplies. Altogether, proliferation of fungi with the genetic capacity for C and N acquisition may contribute to the maintenance of biogeochemical cycling under drought.},
doi = {10.1371/journal.pone.0206441},
journal = {PLoS ONE},
number = 11,
volume = 13,
place = {United States},
year = {2018},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1371/journal.pone.0206441

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Cited by: 1 work
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