Loss of protein synthesis quality control in host-restricted organisms

Melnikov, Sergey V.; van den Elzen, Antonia; Stevens, David L.; Thoreen, Carson C.; Söll, Dieter

doi:10.1073/pnas.1815992115

Title: Loss of protein synthesis quality control in host-restricted organisms

Journal Article · 19 November 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1815992115 · OSTI ID:1482775

Melnikov, Sergey V. ^[1]; van den Elzen, Antonia ^[2]; Stevens, David L. ^[3]; Thoreen, Carson C. ^[2];

^[4]

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511,
Department of Cellular and Molecular Physiology, Yale University, New Haven, CT 06520,
Department of Chemistry, Yale University, New Haven, CT 06511
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511,, Department of Chemistry, Yale University, New Haven, CT 06511

Significance This case study reveals that parasitic and symbiotic lifestyles affect the structure of essential molecular machineries of a living cell. We provide evidence that intracellular parasitism and endosymbiosis cause degeneration of the editing domains in aminoacyl-tRNA synthetases, a defect that is known to cause inaccurate translation of the genetic code. This finding suggests that most intracellular pathogens, including causative agents of human disease, have an unanticipated proteome diversity caused by inaccurate translation of the genetic code. Our finding may change current approaches to the study of proteomes of intracellular parasites, parasite–host interactions, and parasites’ sensitivity to drugs, which cause errors in transcription, translation, and protein folding.

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Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: FG02-98ER20311

OSTI ID:: 1482775

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 49 Vol. 115; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Muller's ratchet
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mistranslation
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Title: Loss of protein synthesis quality control in host-restricted organisms

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