Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna
Abstract
The light-harvesting 2 complex (LH2) of the purple phototrophic bacterium Rhodobacter sphaeroides is a highly efficient, light-harvesting antenna that allows growth under a wide-range of light intensities. In order to expand the spectral range of this antenna complex, we first used a series of competition assays to measure the capacity of the non-native pigments 3-acetyl chlorophyll (Chl) a, Chl d, Chl f or bacteriochlorophyll (BChl) b to replace native BChl a in the B800 binding site of LH2. We then adjusted the B800 site and systematically assessed the binding of non-native pigments. We find that Arg–10 of the LH2 β polypeptide plays a crucial role in binding specificity, by providing a hydrogen-bond to the 3-acetyl group of native and non-native pigments. Reconstituted LH2 complexes harbouring the series of (B)Chls were examined by transient absorption and steady-state fluorescence spectroscopies. Although slowed 10-fold to ~6 ps, energy transfer from Chl a to B850 BChl a remained highly efficient. We measured faster energy-transfer time constants for Chl d (3.5 ps) and Chl f (2.7 ps), which have red-shifted absorption maxima compared to Chl a. BChl b, red-shifted from the native BChl a, gave extremely rapid (≤0.1 ps) transfer. These results show that modifiedmore »
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC); Pennsylvania State Univ., University Park, PA (United States); Washington Univ., St. Louis, MO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; Biotechnology and Biological Sciences Research Council (BBSRC); National Science Foundation (NSF); European Commission Marie Skłodowska-Curie Global Fellowship
- OSTI Identifier:
- 1482755
- Alternate Identifier(s):
- OSTI ID: 1566431
- Grant/Contract Number:
- SC0001035; FG02-94ER20137; BB/M000265/1
- Resource Type:
- Published Article
- Journal Name:
- Biochimica et Biophysica Acta - Bioenergetics
- Additional Journal Information:
- Journal Name: Biochimica et Biophysica Acta - Bioenergetics Journal Volume: 1860 Journal Issue: 3; Journal ID: ISSN 0005-2728
- Publisher:
- Elsevier
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; solar (fuels); photosynthesis (natural and artificial); biofuels (including algae and biomass); bio-inspired; charge transport; membrane; synthesis (novel materials); synthesis (self-assembly); Rhodobacter sphaeroides; LH2; bacteriochlorophyll; chlorophyll; light harvesting; ligand binding; protein engineering
Citation Formats
Swainsbury, David J. K., Faries, Kaitlyn M., Niedzwiedzki, Dariusz M., Martin, Elizabeth C., Flinders, Adam J., Canniffe, Daniel P., Shen, Gaozhong, Bryant, Donald A., Kirmaier, Christine, Holten, Dewey, and Hunter, C. Neil. Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna. Netherlands: N. p., 2018.
Web. doi:10.1016/j.bbabio.2018.11.008.
Swainsbury, David J. K., Faries, Kaitlyn M., Niedzwiedzki, Dariusz M., Martin, Elizabeth C., Flinders, Adam J., Canniffe, Daniel P., Shen, Gaozhong, Bryant, Donald A., Kirmaier, Christine, Holten, Dewey, & Hunter, C. Neil. Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna. Netherlands. https://doi.org/10.1016/j.bbabio.2018.11.008
Swainsbury, David J. K., Faries, Kaitlyn M., Niedzwiedzki, Dariusz M., Martin, Elizabeth C., Flinders, Adam J., Canniffe, Daniel P., Shen, Gaozhong, Bryant, Donald A., Kirmaier, Christine, Holten, Dewey, and Hunter, C. Neil. Fri .
"Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna". Netherlands. https://doi.org/10.1016/j.bbabio.2018.11.008.
@article{osti_1482755,
title = {Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna},
author = {Swainsbury, David J. K. and Faries, Kaitlyn M. and Niedzwiedzki, Dariusz M. and Martin, Elizabeth C. and Flinders, Adam J. and Canniffe, Daniel P. and Shen, Gaozhong and Bryant, Donald A. and Kirmaier, Christine and Holten, Dewey and Hunter, C. Neil},
abstractNote = {The light-harvesting 2 complex (LH2) of the purple phototrophic bacterium Rhodobacter sphaeroides is a highly efficient, light-harvesting antenna that allows growth under a wide-range of light intensities. In order to expand the spectral range of this antenna complex, we first used a series of competition assays to measure the capacity of the non-native pigments 3-acetyl chlorophyll (Chl) a, Chl d, Chl f or bacteriochlorophyll (BChl) b to replace native BChl a in the B800 binding site of LH2. We then adjusted the B800 site and systematically assessed the binding of non-native pigments. We find that Arg–10 of the LH2 β polypeptide plays a crucial role in binding specificity, by providing a hydrogen-bond to the 3-acetyl group of native and non-native pigments. Reconstituted LH2 complexes harbouring the series of (B)Chls were examined by transient absorption and steady-state fluorescence spectroscopies. Although slowed 10-fold to ~6 ps, energy transfer from Chl a to B850 BChl a remained highly efficient. We measured faster energy-transfer time constants for Chl d (3.5 ps) and Chl f (2.7 ps), which have red-shifted absorption maxima compared to Chl a. BChl b, red-shifted from the native BChl a, gave extremely rapid (≤0.1 ps) transfer. These results show that modified LH2 complexes, combined with engineered (B)Chl biosynthesis pathways in vivo, have potential for retaining high efficiency whilst acquiring increased spectral range.},
doi = {10.1016/j.bbabio.2018.11.008},
journal = {Biochimica et Biophysica Acta - Bioenergetics},
number = 3,
volume = 1860,
place = {Netherlands},
year = {Fri Nov 09 00:00:00 EST 2018},
month = {Fri Nov 09 00:00:00 EST 2018}
}
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.bbabio.2018.11.008
https://doi.org/10.1016/j.bbabio.2018.11.008
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 29 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
The petite purple photosynthetic powerpack
journal, March 2009
- Jones, Michael R.
- Biochemical Society Transactions, Vol. 37, Issue 2
Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in Rhodobacter sphaeroides and evidence for the existence of a third distinct class of the enzyme
journal, February 2013
- Canniffe, Daniel P.; Jackson, Philip J.; Hollingshead, Sarah
- Biochemical Journal, Vol. 450, Issue 2
Identification of the Upper Exciton Component of the B850 Bacteriochlorophylls of the LH2 Antenna Complex, Using a B800-Free Mutant of Rhodobacter sphaeroides †
journal, April 1998
- Koolhaas, M. H. C.; Frese, R. N.; Fowler, G. J. S.
- Biochemistry, Vol. 37, Issue 14
A Second and Unusual pucBA Operon of Rhodobacter sphaeroides 2.4.1: Genetics and Function of the Encoded Polypeptides
journal, October 2003
- Zeng, X.; Choudhary, M.; Kaplan, S.
- Journal of Bacteriology, Vol. 185, Issue 20
Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides
journal, October 2014
- Cartron, Michaël L.; Olsen, John D.; Sener, Melih
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 10
Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides : LH2 at 6 Å, LH1 and RC-LH1 at 25 Å 1 1Edited by K. Nagai
journal, October 1998
- Walz, Thomas; Jamieson, Stuart J.; Bowers, Claire M.
- Journal of Molecular Biology, Vol. 282, Issue 4
Efficiency of light energy conversion to hydrogen by the photosynthetic bacterium Rhodobacter sphaeroides
journal, January 1987
- Miyake, J.; Kawamura, S.
- International Journal of Hydrogen Energy, Vol. 12, Issue 3
The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes
journal, August 2006
- Cogdell, Richard J.; Gall, Andrew; Köhler, Jürgen
- Quarterly Reviews of Biophysics, Vol. 39, Issue 3
Insertion of chlorophyll a derivatives into the binding sites of B800 bacteriochlorophyll a in light-harvesting complex 2 from the purple photosynthetic bacterium Rhodoblastus acidophilus
journal, February 2018
- Saga, Yoshitaka; Amari, Kenta; Miyagi, Kanji
- Journal of Photochemistry and Photobiology A: Chemistry, Vol. 353
Versatile design of biohybrid light-harvesting architectures to tune location, density, and spectral coverage of attached synthetic chromophores for enhanced energy capture
journal, March 2014
- Harris, Michelle A.; Jiang, Jianbing; Niedzwiedzki, Dariusz M.
- Photosynthesis Research, Vol. 121, Issue 1
B800→B850 Energy Transfer Mechanism in Bacterial LH2 Complexes Investigated by B800 Pigment Exchange
journal, May 2000
- Herek, J. L.; Fraser, N. J.; Pullerits, T.
- Biophysical Journal, Vol. 78, Issue 5
Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria
journal, March 1979
- Stanier, Roger Y.; Deruelles, Josette; Rippka, Rosmarie
- Microbiology, Vol. 111, Issue 1, p. 1-61
Structure of bacteriochlorophyll b
journal, May 1974
- Scheer, Hugo; Svec, Walter A.; Cope, Ben T.
- Journal of the American Chemical Society, Vol. 96, Issue 11
Chlorophyll Biosynthesis in Bacteria: The Origins of Structural and Functional Diversity
journal, October 2007
- Chew, Aline Gomez Maqueo; Bryant, Donald A.
- Annual Review of Microbiology, Vol. 61, Issue 1
Studies on the Chemical and Photochemical Oxidation of Bacteriochlorophyll 1
journal, October 1966
- Smith, John R. Lindsay; Calvin, Melvin
- Journal of the American Chemical Society, Vol. 88, Issue 19
Probing the B800 bacteriochlorophyll binding site of the accessory light-harvesting complex from Rhodobacter sphaeroides using site-directed mutants. II. A low-temperature spectroscopy study of structural aspects of the pigment-protein conformation
journal, January 1994
- Visschers, Ronald W.; Crielaard, Wim; Fowler, Gregory J. S.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1183, Issue 3
B850 pigment-protein complex of Rhodopseudomonas sphaeroides: Extinction coefficients, circular dichroism, and the reversible binding of bacteriochlorophyll
journal, September 1981
- Clayton, R. K.; Clayton, B. J.
- Proceedings of the National Academy of Sciences, Vol. 78, Issue 9
Dimerization of core complexes as an efficient strategy for energy trapping in Rhodobacter sphaeroides
journal, June 2016
- Chenchiliyan, Manoop; Timpmann, Kõu; Jalviste, Erko
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1857, Issue 6
Chlorophyll d as a major pigment
journal, October 1996
- Miyashita, Hideaki; Ikemoto, Hisato; Kurano, Norihide
- Nature, Vol. 383, Issue 6599
Efficient Energy Transfer from the Carotenoid S2 State in a Photosynthetic Light-Harvesting Complex
journal, February 2001
- Macpherson, Alisdair N.; Arellano, Juan B.; Fraser, Niall J.
- Biophysical Journal, Vol. 80, Issue 2
Reconstitution of Core Light-Harvesting Complexes of Photosynthetic Bacteria Using Chemically Synthesized Polypeptides. 2. Determination of Structural Features That Stabilize Complex Formation and Their Implications for the Structure of the Subunit Complex †
journal, March 1998
- Kehoe, John W.; Meadows, Kelley A.; Parkes-Loach, Pamela S.
- Biochemistry, Vol. 37, Issue 10
Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting
journal, November 2017
- Swainsbury, David J. K.; Martin, Elizabeth C.; Vasilev, Cvetelin
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1858, Issue 11
The effects of lithium dodecyl sulfate and sodium borohydride on the absorption spectrum of the B800–850 light-harvesting complex from Rhodopseudomonas acidophila 7750
journal, October 1987
- Chadwick, Barry. W.; Zhang, Chaoying; Cogdell, Richard J.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 893, Issue 3
Mapping the ultrafast flow of harvested solar energy in living photosynthetic cells
journal, October 2017
- Dahlberg, Peter D.; Ting, Po-Chieh; Massey, Sara C.
- Nature Communications, Vol. 8, Issue 1
Trapping Kinetics in Mutants of the Photosynthetic Purple Bacterium Rhodobacter sphaeroides: Influence of the Charge Separation Rate and Consequences for the Rate-Limiting Step in the Light-Harvesting Process
journal, March 1994
- Beekman, Lucas M. P.; van Mourik, Frank; Jones, Michael R.
- Biochemistry, Vol. 33, Issue 11
Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides
journal, November 2016
- Ipekoğlu, Emre M.; Göçmen, Koray; Öz, Mehmet T.
- Journal of Basic Microbiology, Vol. 57, Issue 3
Membrane invagination in Rhodobacter sphaeroides is initiated at curved regions of the cytoplasmic membrane, then forms both budded and fully detached spherical vesicles: Membrane biogenesis in Rba. sphaeroides
journal, April 2010
- Tucker, Jaimey D.; Siebert, C. Alistair; Escalante, Maryana
- Molecular Microbiology, Vol. 76, Issue 4
Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis.
journal, January 1980
- Broglie, R. M.; Hunter, C. N.; Delepelaire, P.
- Proceedings of the National Academy of Sciences, Vol. 77, Issue 1
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
journal, August 1989
- Porra, R. J.; Thompson, W. A.; Kriedemann, P. E.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 975, Issue 3
Temporally and spectrally resolved subpicosecond energy transfer within the peripheral antenna complex (LH2) and from LH2 to the core antenna complex in photosynthetic purple bacteria.
journal, December 1995
- Hess, S.; Chachisvilis, M.; Timpmann, K.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 26
Probing the bacteriochlorophyll binding site by reconstitution of the light-harvesting complex of Rhodospirillum rubrum with bacteriochlorophyll a analogs
journal, March 1990
- Parkes-Loach, Pamela S.; Michalski, Tomasz J.; Bass, Wendy J.
- Biochemistry, Vol. 29, Issue 12
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
Strong Coupling of Localized Surface Plasmons to Excitons in Light-Harvesting Complexes
journal, October 2016
- Tsargorodska, Anna; Cartron, Michaël L.; Vasilev, Cvetelin
- Nano Letters, Vol. 16, Issue 11
Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800–850 of Rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls
journal, May 1998
- Bandilla, Michael; Ücker, Beate; Ram, Marija
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1364, Issue 3
Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway
journal, February 2015
- Chi, Shuang C.; Mothersole, David J.; Dilbeck, Preston
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1847, Issue 2
Triplet State Energy Transfer Between the Primary Donor and the Carotenoid in Rhodobacter sphaeroides R-26.1 Reaction Centers Exchanged with Modified Bacteriochlorophyll Pigments and Reconstituted with Spheroidene
journal, November 1996
- Frank, Harry A.; Chynwat, Veeradej; Posteraro, Anthony
- Photochemistry and Photobiology, Vol. 64, Issue 5
Enhanced rates of subpicosecond energy transfer in blue-shifted light-harvesting LH2 mutants of Rhodobacter sphaeroides.
journal, July 1994
- Hess, S.; Visscher, K. J.; Pullerits, T.
- Biochemistry, Vol. 33, Issue 27
Reconstitution of Chlorophyll d into the Bacterial Photosynthetic Light-harvesting Protein LH2
journal, August 2018
- Saga, Yoshitaka; Yamashita, Madoka; Imanishi, Michie
- Chemistry Letters, Vol. 47, Issue 8
Plasmon-Enhanced Photocurrent of Photosynthetic Pigment Proteins on Nanoporous Silver
journal, December 2015
- Friebe, Vincent M.; Delgado, Juan D.; Swainsbury, David J. K.
- Advanced Functional Materials, Vol. 26, Issue 2
Reversible Changes in the Structural Features of Photosynthetic Light-Harvesting Complex 2 by Removal and Reconstitution of B800 Bacteriochlorophyll a Pigments
journal, June 2017
- Saga, Yoshitaka; Hirota, Keiya; Asakawa, Hitoshi
- Biochemistry, Vol. 56, Issue 27
Probing structure–function relationships in early events in photosynthesis using a chimeric photocomplex
journal, September 2017
- Nagashima, Kenji V. P.; Sasaki, Mai; Hashimoto, Kanako
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 41
Pigment organization of the B800–850 antenna complex of Rhodopseudomonas sphaeroides
journal, May 1984
- Kramer, Herman J. M.; van Grondelle, Rienk; Hunter, C. Neil
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 765, Issue 2
A resonance Raman investigation of the effect of lithium dodecyl sulfate on the B800–850 light-harvesting protein of Rhodopseudomonas acidophila 7750
journal, July 1988
- Robert, Bruno; Frank, Harry A.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 934, Issue 3
Heterologous Production of the Photosynthetic Reaction Center and Light Harvesting 1 Complexes of the Thermophile Thermochromatium tepidum in the Mesophile Rhodobacter sphaeroides and Thermal Stability of a Hybrid Core Complex
journal, August 2017
- Jun, D.; Huang, V.; Beatty, J. T.
- Applied and Environmental Microbiology, Vol. 83, Issue 20
Extinction coefficient for red-shifted chlorophylls: Chlorophyll d and chlorophyll f
journal, August 2012
- Li, Yaqiong; Scales, Nicholas; Blankenship, Robert E.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 8
Direct Imaging of Protein Organization in an Intact Bacterial Organelle Using High-Resolution Atomic Force Microscopy
journal, November 2016
- Kumar, Sandip; Cartron, Michaël L.; Mullin, Nic
- ACS Nano, Vol. 11, Issue 1
Palette of lipophilic bioconjugatable bacteriochlorins for construction of biohybrid light-harvesting architectures
journal, January 2013
- Reddy, Kanumuri Ramesh; Jiang, Jianbing; Krayer, Michael
- Chemical Science, Vol. 4, Issue 5
Cryo-EM structure of the Blastochloris viridis LH1–RC complex at 2.9 Å
journal, April 2018
- Qian, Pu; Siebert, C. Alistair; Wang, Peiyi
- Nature, Vol. 556, Issue 7700
Theoretical study of excitation transfer from modified B800 rings of the LH II antenna complex of Rps. acidophila
journal, June 2002
- Linnanto, J.; Korppi-Tommola, J. E. I.
- Physical Chemistry Chemical Physics, Vol. 4, Issue 14
Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina
journal, February 2016
- Chen, Guangyu E.; Hitchcock, Andrew; Jackson, Philip J.
- Journal of Bacteriology, Vol. 198, Issue 9
Multireference Excitation Energies for Bacteriochlorophylls A within Light Harvesting System 2
journal, February 2016
- Anda, André; Hansen, Thorsten; De Vico, Luca
- Journal of Chemical Theory and Computation, Vol. 12, Issue 3
Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll–Protein Complex
journal, May 2016
- Hitchcock, Andrew; Jackson, Philip J.; Chidgey, Jack W.
- ACS Synthetic Biology, Vol. 5, Issue 9
Reengineering the Optical Absorption Cross-Section of Photosynthetic Reaction Centers
journal, March 2014
- Dutta, Palash K.; Lin, Su; Loskutov, Andrey
- Journal of the American Chemical Society, Vol. 136, Issue 12
Excitation Energy Transfer between the B850 and B875 Antenna Complexes of Rhodobacter sphaeroides †
journal, February 1997
- Nagarajan, V.; Parson, W. W.
- Biochemistry, Vol. 36, Issue 8
Probing the local lipid environment of the cytochrome bc1 and Synechocystis sp. PCC 6803 cytochrome b6f complexes with styrene maleic acid
journal, March 2018
- Swainsbury, David J. K.; Proctor, Matthew S.; Hitchcock, Andrew
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 3
The Structure and Thermal Motion of the B800–850 LH2 Complex from Rps.acidophila at 2.0Å Resolution and 100K: New Structural Features and Functionally Relevant Motions
journal, March 2003
- Papiz, Miroslav Z.; Prince, Steve M.; Howard, Tina
- Journal of Molecular Biology, Vol. 326, Issue 5
A Red-Shifted Chlorophyll: Fig. 1
journal, August 2010
- Chen, Min; Schliep, Martin; Willows, Robert D.
- Science, Vol. 329, Issue 5997
Top-Down Mass Spectrometry Analysis of Membrane-Bound Light-Harvesting Complex 2 from Rhodobacter sphaeroides
journal, November 2015
- Lu, Yue; Zhang, Hao; Cui, Weidong
- Biochemistry, Vol. 54, Issue 49
Modified reaction centers from Rhodobacter sphaeroides R26: 2: Bacteriochlorophylls with modified C-3 substituents at sites B A and B B
journal, July 1990
- Struck, A.; Cmiel, E.; Katheder, I.
- FEBS Letters, Vol. 268, Issue 1
Probing the B800 bacteriochlorophyll binding site of the accessory light-harvesting complex from Rhodobacter sphaeroides using site-directed mutants. I. Mutagenesis, effects on binding, function and electrochromic behaviour of its carotenoids
journal, January 1994
- Crielaard, Wim; Visschers, Ronald W.; Fowler, Gregory J. S.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1183, Issue 3
Bacteriochlorin-protein interactions in native B800-B850, B800 deficient and B800-Bchl a p -reconstituted complexes from Rhodopseudomonas acidophila , strain 10050
journal, April 1999
- Gall, Andrew; Fraser, Niall J.; Bellissent-Funel, Marie-Claire
- FEBS Letters, Vol. 449, Issue 2-3
Overall energy conversion efficiency of a photosynthetic vesicle
journal, August 2016
- Sener, Melih; Strumpfer, Johan; Singharoy, Abhishek
- eLife, Vol. 5
Influence of the Protein Binding Site on the Absorption Properties of the Monomeric Bacteriochlorophyll in Rhodobacter sphaeroides LH2 Complex †
journal, December 1997
- Gall, Andrew; Fowler, Gregory J. S.; Hunter, C. Neil
- Biochemistry, Vol. 36, Issue 51
Interference lithographic nanopatterning of plant and bacterial light-harvesting complexes on gold substrates
journal, August 2015
- Patole, Samson; Vasilev, Cvetelin; El-Zubir, Osama
- Interface Focus, Vol. 5, Issue 4
Biohybrid Photosynthetic Antenna Complexes for Enhanced Light-Harvesting
journal, February 2012
- Springer, Joseph W.; Parkes-Loach, Pamela S.; Reddy, Kanumuri Ramesh
- Journal of the American Chemical Society, Vol. 134, Issue 10
Modified reaction centers from Rhodobacter sphaeroides R26: Exchange of monomeric bacteriochlorophyll with 13 2 -hydroxy-bacteriochlorophyll
journal, February 1990
- Struck, A.; Scheer, H.
- FEBS Letters, Vol. 261, Issue 2
Engineered biosynthesis of bacteriochlorophyll gF in Rhodobacter sphaeroides
journal, July 2018
- Ortega-Ramos, Marcia; Canniffe, Daniel P.; Radle, Matthew I.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 7
PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroides : Photosystem assembly in
journal, November 2015
- Mothersole, David J.; Jackson, Philip J.; Vasilev, Cvetelin
- Molecular Microbiology, Vol. 99, Issue 2
The Role of βArg - 10 in the B800 Bacteriochlorophyll and Carotenoid Pigment Environment within the Light-Harvesting LH2 Complex of Rhodobacter sphaeroides †
journal, September 1997
- Fowler, Gregory J. S.; Hess, Susan; Pullerits, Tonu
- Biochemistry, Vol. 36, Issue 37
Engineered biosynthesis of bacteriochlorophyll b in Rhodobacter sphaeroides
journal, October 2014
- Canniffe, Daniel P.; Hunter, C. Neil
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 10
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum
journal, July 1994
- Schäfer, Andreas; Tauch, Andreas; Jäger, Wolfgang
- Gene, Vol. 145, Issue 1, p. 69-73
Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre
journal, January 2017
- Grayson, Katie J.; Faries, Kaitlyn M.; Huang, Xia
- Nature Communications, Vol. 8, Issue 1
Extension of Light-Harvesting Ability of Photosynthetic Light-Harvesting Complex 2 (LH2) through Ultrafast Energy Transfer from Covalently Attached Artificial Chromophores
journal, September 2015
- Yoneda, Yusuke; Noji, Tomoyasu; Katayama, Tetsuro
- Journal of the American Chemical Society, Vol. 137, Issue 40
Integration of multiple chromophores with native photosynthetic antennas to enhance solar energy capture and delivery
journal, January 2013
- Harris, Michelle A.; Parkes-Loach, Pamela S.; Springer, Joseph W.
- Chemical Science, Vol. 4, Issue 10
Selective Release, Removal, and Reconstitution of Bacteriochlorophyll a Molecules into the B800 Sites of LH2 Complexes from Rhodopseudomonas acidophila 10050 †
journal, July 1999
- Fraser, Niall J.; Dominy, Peter J.; Ücker, Beaté
- Biochemistry, Vol. 38, Issue 30
Probing the binding sites of exchanged chlorophyll a in LH2 by Raman and site-selection fluorescence spectroscopies
journal, February 2001
- Gall, Andrew; Robert, Bruno; Cogdell, Richard J.
- FEBS Letters, Vol. 491, Issue 1-2
The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides A modified Q-cycle mechanism
journal, May 1983
- Crofts, A. R.; Meinhardt, S. W.; Jones, K. R.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 723, Issue 2
Composition and optical properties of reaction centre core complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum
journal, April 2000
- Permentier, Hjalmar P.
- Photosynthesis Research, Vol. 64, Issue 1, p. 27-39