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Title: Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component

There is an increasing appreciation for structural diversity of DNA that is of interest to both DNA nanotechnology and basic biology. Here, we have explored how DNA responds to torsional stress by building on a previously reported two-turn DNA tensegrity triangle and demonstrating that we could introduce an extra nucleotide pair (np) into the original sequence without affecting assembly and crystallization. The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice. Furthermore, the work reveals a near-atomic structure of naked DNA under a torsional stress of approximately 14%, and thus provides an example of DNA distortions that occur without a requirement for either an external energy source or the free energy available from protein or drug binding.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1]
  1. New York Univ., New York, NY (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States)
  3. Purdue Univ., West Lafayette, IN (United States)
Publication Date:
Report Number(s):
BNL-203483-2018-JAAM
Journal ID: ISSN 2451-9456
Grant/Contract Number:
SC0012704; SC0007991
Type:
Published Article
Journal Name:
Cell Chemical Biology
Additional Journal Information:
Journal Volume: 24; Journal Issue: 11; Journal ID: ISSN 2451-9456
Publisher:
Cell Press - Elsevier
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; torsionally stressed DNA; structural DNA nanotechnology; self-assembled 3D; DNA crystals; X-ray diffraction; designed crystals
OSTI Identifier:
1482398
Alternate Identifier(s):
OSTI ID: 1435732

Hernandez, Carina, Birktoft, Jens J., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Abdallah, Hatem, Sha, Ruojie, Stojanoff, Vivian, Mao, Chengde, and Seeman, Nadrian C.. Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component. United States: N. p., Web. doi:10.1016/j.chembiol.2017.08.018.
Hernandez, Carina, Birktoft, Jens J., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Abdallah, Hatem, Sha, Ruojie, Stojanoff, Vivian, Mao, Chengde, & Seeman, Nadrian C.. Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component. United States. doi:10.1016/j.chembiol.2017.08.018.
Hernandez, Carina, Birktoft, Jens J., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Abdallah, Hatem, Sha, Ruojie, Stojanoff, Vivian, Mao, Chengde, and Seeman, Nadrian C.. 2017. "Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component". United States. doi:10.1016/j.chembiol.2017.08.018.
@article{osti_1482398,
title = {Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component},
author = {Hernandez, Carina and Birktoft, Jens J. and Ohayon, Yoel P. and Chandrasekaran, Arun Richard and Abdallah, Hatem and Sha, Ruojie and Stojanoff, Vivian and Mao, Chengde and Seeman, Nadrian C.},
abstractNote = {There is an increasing appreciation for structural diversity of DNA that is of interest to both DNA nanotechnology and basic biology. Here, we have explored how DNA responds to torsional stress by building on a previously reported two-turn DNA tensegrity triangle and demonstrating that we could introduce an extra nucleotide pair (np) into the original sequence without affecting assembly and crystallization. The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice. Furthermore, the work reveals a near-atomic structure of naked DNA under a torsional stress of approximately 14%, and thus provides an example of DNA distortions that occur without a requirement for either an external energy source or the free energy available from protein or drug binding.},
doi = {10.1016/j.chembiol.2017.08.018},
journal = {Cell Chemical Biology},
number = 11,
volume = 24,
place = {United States},
year = {2017},
month = {10}
}