Self-Assembly of a Designed Nucleoprotein Architecture through Multimodal Interactions
Abstract
The co-self-assembly of proteins and nucleic acids (NAs) produces complex biomolecular machines (e.g., ribosomes and telomerases) that represent some of the most daunting targets for biomolecular design. Despite significant advances in protein and DNA or RNA nanotechnology, the construction of artificial nucleoprotein complexes has largely been limited to cases that rely on the NA-mediated spatial organization of protein units, rather than a cooperative interplay between protein- and NA-mediated interactions that typify natural nucleoprotein assemblies. We report here a structurally well-defined synthetic nucleoprotein assembly that forms through the synergy of three types of intermolecular interactions: Watson–Crick base pairing, NA–protein interactions, and protein–metal coordination. The fine thermodynamic balance between these interactions enables the formation of a crystalline architecture under highly specific conditions.
- Authors:
-
[1];
[1];
[4]
- Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States
- C−CINA, Biozentrum, University of Basel, Mattenstrasse 26, 4058 Basel, Switzerland
- Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, United States
- Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States, Materials Science and Engineering, University of California, San Diego, La Jolla, California 92093, United States
- Publication Date:
- Research Org.:
- Univ. of California, San Diego, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); National Inst. of Health (NIH) (United States)
- OSTI Identifier:
- 1482287
- Alternate Identifier(s):
- OSTI ID: 1508780
- Grant/Contract Number:
- SC0003844; AC02-76SF00515; DMR-1602537; T32GM112584-01; R01-GM033050
- Resource Type:
- Published Article
- Journal Name:
- ACS Central Science
- Additional Journal Information:
- Journal Name: ACS Central Science Journal Volume: 4 Journal Issue: 11; Journal ID: ISSN 2374-7943
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Subramanian, Rohit H., Smith, Sarah J., Alberstein, Robert G., Bailey, Jake B., Zhang, Ling, Cardone, Giovanni, Suominen, Lauri, Chami, Mohamed, Stahlberg, Henning, Baker, Timothy S., and Tezcan, F. Akif. Self-Assembly of a Designed Nucleoprotein Architecture through Multimodal Interactions. United States: N. p., 2018.
Web. doi:10.1021/acscentsci.8b00745.
Subramanian, Rohit H., Smith, Sarah J., Alberstein, Robert G., Bailey, Jake B., Zhang, Ling, Cardone, Giovanni, Suominen, Lauri, Chami, Mohamed, Stahlberg, Henning, Baker, Timothy S., & Tezcan, F. Akif. Self-Assembly of a Designed Nucleoprotein Architecture through Multimodal Interactions. United States. https://doi.org/10.1021/acscentsci.8b00745
Subramanian, Rohit H., Smith, Sarah J., Alberstein, Robert G., Bailey, Jake B., Zhang, Ling, Cardone, Giovanni, Suominen, Lauri, Chami, Mohamed, Stahlberg, Henning, Baker, Timothy S., and Tezcan, F. Akif. Thu .
"Self-Assembly of a Designed Nucleoprotein Architecture through Multimodal Interactions". United States. https://doi.org/10.1021/acscentsci.8b00745.
@article{osti_1482287,
title = {Self-Assembly of a Designed Nucleoprotein Architecture through Multimodal Interactions},
author = {Subramanian, Rohit H. and Smith, Sarah J. and Alberstein, Robert G. and Bailey, Jake B. and Zhang, Ling and Cardone, Giovanni and Suominen, Lauri and Chami, Mohamed and Stahlberg, Henning and Baker, Timothy S. and Tezcan, F. Akif},
abstractNote = {The co-self-assembly of proteins and nucleic acids (NAs) produces complex biomolecular machines (e.g., ribosomes and telomerases) that represent some of the most daunting targets for biomolecular design. Despite significant advances in protein and DNA or RNA nanotechnology, the construction of artificial nucleoprotein complexes has largely been limited to cases that rely on the NA-mediated spatial organization of protein units, rather than a cooperative interplay between protein- and NA-mediated interactions that typify natural nucleoprotein assemblies. We report here a structurally well-defined synthetic nucleoprotein assembly that forms through the synergy of three types of intermolecular interactions: Watson–Crick base pairing, NA–protein interactions, and protein–metal coordination. The fine thermodynamic balance between these interactions enables the formation of a crystalline architecture under highly specific conditions.},
doi = {10.1021/acscentsci.8b00745},
journal = {ACS Central Science},
number = 11,
volume = 4,
place = {United States},
year = {2018},
month = {11}
}
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https://doi.org/10.1021/acscentsci.8b00745
https://doi.org/10.1021/acscentsci.8b00745
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Cited by: 2 works
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Figures / Tables:
Figure 1: Design of RIDC3−10a/b. (a) Schematic of RIDC3−DNA hybrids. Metal-binding residues are shown as cyan sticks on the RIDC3 surface. (b) Potential self-assembly outcomes for the RIDC3−DNA hybrids.
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Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.