Isolation and Whole-Genome Sequencing of Environmental Campylobacter
Abstract
As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter has a significant impact on human health. In the developed world, most campylobacteriosis cases are attributed to the consumption of undercooked, contaminated poultry; however, it has been shown that Campylobacter can be transmitted to humans through contaminated water and other types of food, including beef and milk. As such, high-resolution microbial source-tracking is essential for health department officials to determine the source(s) of Campylobacter outbreaks. For these reasons, this paper provides the techniques needed for isolation of Campylobacter from agricultural and environmental sources, as well as human clinical specimens. Additionally, we describe a simple method for preparing high-quality genomic DNA that can be used for whole-genome sequencing and downstream bioinformatics analyses of Campylobacter genotypes.
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Univ. of Tennessee, Knoxville, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); ORNL Laboratory Directed Research and Development (LDRD) Program; Univ. of Tennessee Joint Directed Research and Development (JDRD) Program
- OSTI Identifier:
- 1481691
- Alternate Identifier(s):
- OSTI ID: 1479577
- Grant/Contract Number:
- AC05-00OR22725; R013318090
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Current Protocols in Microbiology
- Additional Journal Information:
- Journal Volume: 51; Journal Issue: 1; Journal ID: ISSN 1934-8525
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Campylobacter; environmental isolation; whole-genome sequencing
Citation Formats
Kelley, Brittni R., Ellis, J. Christopher, Hyatt, Doug, Jacobson, Dan, and Johnson, Jeremiah. Isolation and Whole-Genome Sequencing of Environmental Campylobacter. United States: N. p., 2018.
Web. doi:10.1002/cpmc.64.
Kelley, Brittni R., Ellis, J. Christopher, Hyatt, Doug, Jacobson, Dan, & Johnson, Jeremiah. Isolation and Whole-Genome Sequencing of Environmental Campylobacter. United States. doi:https://doi.org/10.1002/cpmc.64
Kelley, Brittni R., Ellis, J. Christopher, Hyatt, Doug, Jacobson, Dan, and Johnson, Jeremiah. Thu .
"Isolation and Whole-Genome Sequencing of Environmental Campylobacter". United States. doi:https://doi.org/10.1002/cpmc.64. https://www.osti.gov/servlets/purl/1481691.
@article{osti_1481691,
title = {Isolation and Whole-Genome Sequencing of Environmental Campylobacter},
author = {Kelley, Brittni R. and Ellis, J. Christopher and Hyatt, Doug and Jacobson, Dan and Johnson, Jeremiah},
abstractNote = {As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter has a significant impact on human health. In the developed world, most campylobacteriosis cases are attributed to the consumption of undercooked, contaminated poultry; however, it has been shown that Campylobacter can be transmitted to humans through contaminated water and other types of food, including beef and milk. As such, high-resolution microbial source-tracking is essential for health department officials to determine the source(s) of Campylobacter outbreaks. For these reasons, this paper provides the techniques needed for isolation of Campylobacter from agricultural and environmental sources, as well as human clinical specimens. Additionally, we describe a simple method for preparing high-quality genomic DNA that can be used for whole-genome sequencing and downstream bioinformatics analyses of Campylobacter genotypes.},
doi = {10.1002/cpmc.64},
journal = {Current Protocols in Microbiology},
number = 1,
volume = 51,
place = {United States},
year = {2018},
month = {10}
}
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