Fluvastatin protects cochleae from damage by high-level noise

Richter, Claus-Peter; Young, Hunter; Richter, Sonja V.; Smith-Bronstein, Virginia; Stock, Stuart R.; Xiao, Xianghui; Soriano, Carmen; Whitlon, Donna S.

doi:10.1038/s41598-018-21336-7

Title: Fluvastatin protects cochleae from damage by high-level noise

Journal Article · 14 February 2018 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-018-21336-7 · OSTI ID:1481415

^[1]; Young, Hunter ^[2]; Richter, Sonja V. ^[2]; Smith-Bronstein, Virginia ^[2]; Stock, Stuart R. ^[3]; Xiao, Xianghui ^[4]; Soriano, Carmen ^[4]; Whitlon, Donna S. ^[5]

Northwestern University Feinberg School of Medicine, Chicago, IL (United States). Department of Otolaryngology; Northwestern Univ., Evanston, IL (United States). Department of Biomedical Engineering and The Hugh Knowles Center, Department of Communication Sciences and Disorders
Northwestern University Feinberg School of Medicine, Chicago, IL (United States). Department of Otolaryngology
Northwestern University Feinberg School of Medicine, Chicago, IL (United States). Department of Cell and Molecular Biology
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Northwestern University Feinberg School of Medicine, Chicago, IL (United States). Department of Otolaryngology; Northwestern Univ., Evanston, IL (United States). The Hugh Knowles Center, Department of Communication Sciences and Disorders and Interdepartmental Neurosciences Program

Exposure to noise and ototoxic drugs are responsible for much of the debilitating hearing loss experienced by about 350 million people worldwide. Beyond hearing aids and cochlear implants, there have been no other FDA approved drug interventions established in the clinic that would either protect or reverse the effects of hearing loss. Using Auditory Brainstem Responses (ABR) in a guinea pig model, we demonstrate that fluvastatin, an inhibitor of HMG-CoA reductase, the rate-limiting enzyme of the mevalonate pathway, protects against loss of cochlear function initiated by high intensity noise. A novel synchrotron radiation based X-ray tomographic method that imaged soft tissues at micrometer resolution in unsectioned cochleae, allowed an efficient, qualitative evaluation of the three-dimensional internal structure of the intact organ. For quantitative measures, plastic embedded cochleae were sectioned followed by hair cell counting. Protection in noise-exposed cochleae is associated with retention of inner and outer hair cells. This study demonstrates the potential of HMG-CoA reductase inhibitors, already vetted in human medicine for other purposes, to protect against noise induced hearing loss.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); US Department of the Navy, Office of Naval Research (ONR)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1481415

Journal Information:: Scientific Reports, Vol. 8, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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References (118)

The genetics of deafness Nance, Walter E. Mental Retardation and Developmental Disabilities Research Reviews, Vol. 9, Issue 2 https://doi.org/10.1002/mrdd.10067	journal	January 2003
The anatomical consequences of acoustic injury: A review and tutorial Saunders, James C.; Dear, Steven P.; Schneider, Mark E. The Journal of the Acoustical Society of America, Vol. 78, Issue 3 https://doi.org/10.1121/1.392915	journal	September 1985
Histology and synchrotron radiation-based microtomography of the inner ear in a molecularly confirmed case of CHARGE syndrome Glueckert, Rudolf; Rask-Andersen, Helge; Sergi, Consolato American Journal of Medical Genetics Part A, Vol. 152A, Issue 3 https://doi.org/10.1002/ajmg.a.33321	journal	March 2010
Prestin-Based Outer Hair Cell Motility Is Necessary for Mammalian Cochlear Amplification Dallos, Peter; Wu, Xudong; Cheatham, Mary Ann Neuron, Vol. 58, Issue 3 https://doi.org/10.1016/j.neuron.2008.02.028	journal	May 2008
Pravastatin attenuates noise-induced cochlear injury in mice Park, J. S.; Kim, S. W.; Park, K. Neuroscience, Vol. 208 https://doi.org/10.1016/j.neuroscience.2012.02.010	journal	April 2012
Simvastatin treatment induces morphology alterations and apoptosis in murine cochlear neuronal cells Park, Do-Sim; So, Hong-Seob; Lee, Jeong-Han Acta Oto-Laryngologica, Vol. 129, Issue 2 https://doi.org/10.1080/00016480802163358	journal	January 2009
Acoustic Damage of the Cochlea: A Scanning and Transmission Electron Microscopic Observation Lim, D. J.; Melnick, W. Archives of Otolaryngology - Head and Neck Surgery, Vol. 94, Issue 4 https://doi.org/10.1001/archotol.1971.00770070486002	journal	October 1971
Intracellular labeling of auditory nerve fibers in guinea pig: central and peripheral projections Tsuji, J.; Liberman, M. C. The Journal of Comparative Neurology, Vol. 381, Issue 2 https://doi.org/10.1002/(SICI)1096-9861(19970505)381:2<188::AID-CNE6>3.0.CO;2-#	journal	May 1997
Electrokinetic shape changes of cochlear outer hair cells Kachar, Bechara; Brownell, William E.; Altschuler, Richard Nature, Vol. 322, Issue 6077 https://doi.org/10.1038/322365a0	journal	July 1986
Auditory and non-auditory effects of noise on health Basner, Mathias; Babisch, Wolfgang; Davis, Adrian The Lancet, Vol. 383, Issue 9925 https://doi.org/10.1016/S0140-6736(13)61613-X	journal	April 2014
The Prevalence of Hearing Impairment and Associated Risk Factors: The Beaver Dam Offspring Study Nash, Scott D.; Cruickshanks, Karen J.; Klein, Ronald Archives of Otolaryngology–Head & Neck Surgery, Vol. 137, Issue 5 https://doi.org/10.1001/archoto.2011.15	journal	May 2011
Noise-induced hearing loss: an occupational medicine perspective Stucken, Emily Z.; Hong, Robert S. Current Opinion in Otolaryngology & Head and Neck Surgery, Vol. 22, Issue 5 https://doi.org/10.1097/MOO.0000000000000079	journal	January 2014
Free Radicals in the Guinea Pig Inner Ear following Gentamicin Exposure Takumida, Masaya; Popa, Raul; Anniko, Matti ORL, Vol. 61, Issue 2 https://doi.org/10.1159/000027643	journal	January 1999
Early investigational drugs for hearing loss Mukherjea, Debashree; Ghosh, Sumana; Bhatta, Puspanjali Expert Opinion on Investigational Drugs, Vol. 24, Issue 2 https://doi.org/10.1517/13543784.2015.960076	journal	September 2014
An Overview of Hereditary Hearing Loss Bayazit, Yildirim A.; Yılmaz, Metin ORL, Vol. 68, Issue 2 https://doi.org/10.1159/000091090	journal	January 2006
Contribution of genetic factors to noise-induced hearing loss: A human studies review Sliwinska-Kowalska, Mariola; Pawelczyk, Malgorzata Mutation Research/Reviews in Mutation Research, Vol. 752, Issue 1 https://doi.org/10.1016/j.mrrev.2012.11.001	journal	January 2013
Renexin as a rescue regimen for noise-induced hearing loss Park, ShiNae; Yeo, SangWon; Back, SangA Noise and Health, Vol. 16, Issue 72 https://doi.org/10.4103/1463-1741.140497	journal	January 2014
Surdités d’origine génétique Marcolla, A.; Bouchetemble, P.; Lerosey, Y. Annales d'Otolaryngologie et de Chirurgie Cervico-faciale, Vol. 123, Issue 3 https://doi.org/10.1016/S0003-438X(06)76655-5	journal	June 2006
Quantitative X-ray Tomography of the Mouse Cochlea Rau, Christoph; Hwang, Margaret; Lee, Wah-Keat PLoS ONE, Vol. 7, Issue 4 https://doi.org/10.1371/journal.pone.0033568	journal	April 2012
Novel High Content Screen Detects Compounds That Promote Neurite Regeneration from Cochlear Spiral Ganglion Neurons Whitlon, Donna S.; Grover, Mary; Dunne, Sara F. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep15960	journal	November 2015
Atorvastatin slows down the deterioration of inner ear function with age in mice Syka, Josef; Ouda, Ladislav; Nachtigal, Petr Neuroscience Letters, Vol. 411, Issue 2 https://doi.org/10.1016/j.neulet.2006.10.032	journal	January 2007
Transduction of the contralateral ear after adenovirus-mediated cochlear gene transfer Stöver, T.; Yagi, M.; Raphael, Y. Gene Therapy, Vol. 7, Issue 5 https://doi.org/10.1038/sj.gt.3301108	journal	February 2000
How the ear's works work: mechanoelectrical transduction and amplification by hair cells Hudspeth, A. J. Comptes Rendus Biologies, Vol. 328, Issue 2 https://doi.org/10.1016/j.crvi.2004.12.003	journal	February 2005
Evoked mechanical responses of isolated cochlear outer hair cells Brownell, W.; Bader, C.; Bertrand, D. Science, Vol. 227, Issue 4683 https://doi.org/10.1126/science.3966153	journal	January 1985
Morphological Characterisation of Unstained and Intact Tissue Micro-architecture by X-ray Computed Micro- and Nano-Tomography Walton, Lucy A.; Bradley, Robert S.; Withers, Philip J. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep10074	journal	May 2015
Effect of Atorvastatin on Progression of Sensorineural Hearing Loss and Tinnitus in the Elderly: Results of a Prospective, Randomized, Double-Blind Clinical Trial Olzowy, Bernhard; Canis, Martin; Hempel, John-Martin Otology & Neurotology, Vol. 28, Issue 4 https://doi.org/10.1097/01.mao.0000271673.33683.7b	journal	January 2007
Functional and morphological changes in the cochlea of cholesterolfed guinea pigs. [高脂食負荷モルモット内耳か牛の機能的，形態学的変化] Kashiwako, Izumi; Hattori, Yasuo; Qiao, Yan Journal of Nippon Medical School, Vol. 61, Issue 4 https://doi.org/10.1272/jnms1923.61.321	journal	January 1994
Alterations of inner ear morphology in experimental hypercholesterolemia Gratton, Michael Anne; Wright, Charles G. Hearing Research, Vol. 61, Issue 1-2 https://doi.org/10.1016/0378-5955(92)90040-T	journal	August 1992
Properties of auditory nerve responses in absence of outer hair cells Dallos, P.; Harris, D. Journal of Neurophysiology, Vol. 41, Issue 2 https://doi.org/10.1152/jn.1978.41.2.365	journal	March 1978
High-resolution X-ray tomography of the human inner ear: synchrotron radiation-based study of nerve fibre bundles, membranes and ganglion cells Lareida, A.; Beckmann, F.; Schrott-Fischer, A. Journal of Microscopy, Vol. 234, Issue 1 https://doi.org/10.1111/j.1365-2818.2009.03143.x	journal	April 2009
Communication pathways to and from the inner ear and their contributions to drug delivery Salt, Alec N.; Hirose, Keiko Hearing Research, Vol. 362 https://doi.org/10.1016/j.heares.2017.12.010	journal	May 2018
Genetic diseases of hearing Spillmann, Thomas Current Opinion in Neurology, Vol. 7, Issue 1 https://doi.org/10.1097/00019052-199402000-00015	journal	January 1994
Cochlear pathology of long term neomycin induced deafness in cats Leake, Patricia A.; Hradek, Gary T. Hearing Research, Vol. 33, Issue 1 https://doi.org/10.1016/0378-5955(88)90018-4	journal	April 1988
The remarkable cochlear amplifier Ashmore, J.; Avan, P.; Brownell, W. E. Hearing Research, Vol. 266, Issue 1-2 https://doi.org/10.1016/j.heares.2010.05.001	journal	July 2010
Emerging treatments for noise-induced hearing loss Oishi, Naoki; Schacht, Jochen Expert Opinion on Emerging Drugs, Vol. 16, Issue 2 https://doi.org/10.1517/14728214.2011.552427	journal	January 2011
Acoustic injury and the physiology of hearing Schmiedt, Richard A. The Journal of the Acoustical Society of America, Vol. 76, Issue 5 https://doi.org/10.1121/1.391446	journal	November 1984
Genetic investigations in childhood deafness Parker, Michael; Bitner-Glindzicz, Maria Archives of Disease in Childhood, Vol. 100, Issue 3 https://doi.org/10.1136/archdischild-2014-306099	journal	October 2014
TomoPy: a framework for the analysis of synchrotron tomographic data Gürsoy, Dogˇa; De Carlo, Francesco; Xiao, Xianghui Journal of Synchrotron Radiation, Vol. 21, Issue 5 https://doi.org/10.1107/S1600577514013939	journal	August 2014
Imaging of cochlear tissue with a grating interferometer and hard X-rays Richter, Claus-Peter; Shintani-Smith, Stephanie; Fishman, Andrew Microscopy Research and Technique, Vol. 72, Issue 12 https://doi.org/10.1002/jemt.20728	journal	December 2009
Time Sequence of Degeneration Pattern of the Organ of Corti after Acoustic Overstimulation: A Transmission Electron Microscopy Study Fredelius, Lars Acta Oto-Laryngologica, Vol. 106, Issue 5-6 https://doi.org/10.3109/00016488809122260	journal	January 1988
Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object Paganin, D.; Mayo, S. C.; Gureyev, T. E. Journal of Microscopy, Vol. 206, Issue 1 https://doi.org/10.1046/j.1365-2818.2002.01010.x	journal	April 2002
Gentamicin uptake in the chinchilla inner ear Roehm, Pamela; Hoffer, Michael; Balaban, Carey D. Hearing Research, Vol. 230, Issue 1-2 https://doi.org/10.1016/j.heares.2007.04.005	journal	August 2007
Direct MicroCT imaging of non-mineralized connective tissues at high resolution Naveh, Gili R. S.; Brumfeld, Vlad; Dean, Mason Connective Tissue Research, Vol. 55, Issue 1 https://doi.org/10.3109/03008207.2013.867333	journal	January 2014
Primary Neural Degeneration in the Guinea Pig Cochlea After Reversible Noise-Induced Threshold Shift Lin, Harrison W.; Furman, Adam C.; Kujawa, Sharon G. Journal of the Association for Research in Otolaryngology, Vol. 12, Issue 5 https://doi.org/10.1007/s10162-011-0277-0	journal	June 2011
Comparative Adverse Effect Profiles of Platinum Drugs McKeage, Mark J. Drug Safety, Vol. 13, Issue 4 https://doi.org/10.2165/00002018-199513040-00003	journal	January 1995
Reactive Oxygen Species, Apoptosis, and Mitochondrial Dysfunction in Hearing Loss Kamogashira, Teru; Fujimoto, Chisato; Yamasoba, Tatsuya BioMed Research International, Vol. 2015 https://doi.org/10.1155/2015/617207	journal	January 2015
Toxicology of blast overpressure Elsayed, Nabil M. Toxicology, Vol. 121, Issue 1 https://doi.org/10.1016/S0300-483X(97)03651-2	journal	July 1997
Music exposure and hearing disorders: An overview Zhao, Fei; Manchaiah, Vinaya K. C.; French, David International Journal of Audiology, Vol. 49, Issue 1 https://doi.org/10.3109/14992020903202520	journal	December 2009
Nitrones as therapeutics Floyd, Robert A.; Kopke, Richard D.; Choi, Chul-Hee Free Radical Biology and Medicine, Vol. 45, Issue 10 https://doi.org/10.1016/j.freeradbiomed.2008.08.017	journal	November 2008
Safety of Adeno-Associated Virus as Cochlear Gene Transfer Vector: Analysis of Distant Spread Beyond Injected Cochleae Kho, Soochuen T.; Pettis, Robert M.; Mhatre, Anand N. Molecular Therapy, Vol. 2, Issue 4 https://doi.org/10.1006/mthe.2000.0129	journal	October 2000
Possible role of HMG-CoA reductase inhibitors for the treatment of sudden sensorineural hearing loss (SSHL) Borghi, C.; Modugno, G. C.; Pirodda, A. Medical Hypotheses, Vol. 58, Issue 5 https://doi.org/10.1054/mehy.2001.1535	journal	May 2002
Sensorineural hearing loss in combined modality treatment of nasopharyngeal carcinoma Chen, William C.; Jackson, Andrew; Budnick, Amy S. Cancer, Vol. 106, Issue 4 https://doi.org/10.1002/cncr.21683	journal	January 2006
Sex-linked deafness Petersen, Mb; Wang, Q.; Willems, Pj Clinical Genetics, Vol. 73, Issue 1 https://doi.org/10.1111/j.1399-0004.2007.00913.x	journal	November 2007
Visualizing soft tissue in the mammalian cochlea with coherent hard X-rays Rau, C.; Robinson, I. K.; Richter, C. -P. Microscopy Research and Technique, Vol. 69, Issue 8 https://doi.org/10.1002/jemt.20336	journal	January 2006
The structural and functional consequences of acoustic injury in the cochlea and peripheral auditory system: A five year update Saunders, James C.; Cohen, Yale E.; Szymko, Yvonne M. The Journal of the Acoustical Society of America, Vol. 90, Issue 1 https://doi.org/10.1121/1.401307	journal	July 1991
Targeted Mutation of the Gene for Cellular Glutathione Peroxidase (Gpx1) Increases Noise-Induced Hearing Loss in Mice Ohlemiller, Kevin K.; McFadden, Sandra L.; Ding, Da-Lian Journal of the Association for Research in Otolaryngology, Vol. 1, Issue 3 https://doi.org/10.1007/s101620010043	journal	September 2000
Is the din really harmless? Long-term effects of non-traumatic noise on the adult auditory system Gourévitch, Boris; Edeline, Jean-Marc; Occelli, Florian Nature Reviews Neuroscience, Vol. 15, Issue 7 https://doi.org/10.1038/nrn3744	journal	June 2014
Death pathways in noise-damaged outer hair cells Bohne, Barbara A.; Harding, Gary W.; Lee, Steve C. Hearing Research, Vol. 223, Issue 1-2 https://doi.org/10.1016/j.heares.2006.10.004	journal	January 2007
ANKAphase : software for single-distance phase retrieval from inline X-ray phase-contrast radiographs Weitkamp, T.; Haas, D.; Wegrzynek, D. Journal of Synchrotron Radiation, Vol. 18, Issue 4 https://doi.org/10.1107/S0909049511002895	journal	March 2011
Genetics of hearing loss: where are we standing now? Mahboubi, Hossein; Dwabe, Sami; Fradkin, Matthew European Archives of Oto-Rhino-Laryngology, Vol. 269, Issue 7 https://doi.org/10.1007/s00405-011-1910-6	journal	January 2012
Time course of efferent fiber and spiral ganglion cell degeneration following complete hair cell loss in the chinchilla McFadden, Sandra L.; Ding, Dalian; Jiang, Haiyan Brain Research, Vol. 997, Issue 1 https://doi.org/10.1016/j.brainres.2003.10.031	journal	January 2004
Damage of the Auditory System Associated with Acute Blast Trauma Roberto, Michele; Hamernik, Roger P.; Turrentine, George A. Annals of Otology, Rhinology & Laryngology, Vol. 98, Issue 5_suppl https://doi.org/10.1177/00034894890980S506	journal	May 1989
Prestin, the Motor Protein of Outer Hair Cells Zheng, Jing; Madison, Laird D.; Oliver, Dominik Audiology and Neurotology, Vol. 7, Issue 1 https://doi.org/10.1159/000046855	journal	January 2002
How the ear's works work Hudspeth, A. J. Nature, Vol. 341, Issue 6241 https://doi.org/10.1038/341397a0	journal	October 1989
Identifying components of the hair-cell interactome involved in cochlear amplification Zheng, Jing; Anderson, Charles T.; Miller, Katharine K. BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-127	journal	January 2009
Simvastatin protects auditory hair cells from gentamicin-induced toxicity and activates Akt signaling in vitro Brand, Yves; Setz, Cristian; Levano, Soledad BMC Neuroscience, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2202-12-114	journal	January 2011
Noise-Induced Hearing loss Wheeler, D. E. Archives of Otolaryngology - Head and Neck Surgery, Vol. 51, Issue 3 https://doi.org/10.1001/archotol.1950.00700020366006	journal	March 1950
Effect of Atorvastatin on Progression of Sensorineural Hearing Loss and Tinnitus in the Elderly: Results of a Prospective, Randomized, Double-Blind Clinical Trial Olzowy, Bernhard; Canis, Martin; Hempel, John-Martin Otology & Neurotology, Vol. PAP https://doi.org/10.1097/01.mao.0000265195.16507.08	journal	January 2007
TomoPy: A framework for the analysis of synchrotron tomographic data Gürsoy, Doğa; De Carlo, Francesco; Xiao, Xianghui SPIE Optical Engineering + Applications, SPIE Proceedings https://doi.org/10.1117/12.2061373	conference	September 2014
Noise-Induced Hearing Loss: Prevention in the Work Environment Tortora, Mary Lou AAOHN Journal, Vol. 35, Issue 6 https://doi.org/10.1177/216507998703500606	journal	June 1987
The genetics of deafness Nance, Walter E. American Journal of Medical Genetics, Vol. 130A, Issue 1 https://doi.org/10.1002/ajmg.a.30055	journal	January 2004
The Prevalence of Hearing Impairment and Associated Risk Factors: The Beaver Dam Offspring Study Nash, Scott D.; Cruickshanks, Karen J.; Klein, Ronald Archives of Otolaryngology–Head & Neck Surgery, Vol. 137, Issue 5 https://doi.org/10.1001/archoto.2011.15	journal	May 2011
Acoustic Damage of the Cochlea: A Scanning and Transmission Electron Microscopic Observation Lim, D. J.; Melnick, W. Archives of Otolaryngology - Head and Neck Surgery, Vol. 94, Issue 4 https://doi.org/10.1001/archotol.1971.00770070486002	journal	October 1971
The genetics of deafness Nance, Walter E. American Journal of Medical Genetics, Vol. 130A, Issue 1 https://doi.org/10.1002/ajmg.a.30055	journal	January 2004
Histology and synchrotron radiation-based microtomography of the inner ear in a molecularly confirmed case of CHARGE syndrome Glueckert, Rudolf; Rask-Andersen, Helge; Sergi, Consolato American Journal of Medical Genetics Part A, Vol. 152A, Issue 3 https://doi.org/10.1002/ajmg.a.33321	journal	March 2010
Sensorineural hearing loss in combined modality treatment of nasopharyngeal carcinoma Chen, William C.; Jackson, Andrew; Budnick, Amy S. Cancer, Vol. 106, Issue 4 https://doi.org/10.1002/cncr.21683	journal	January 2006
Visualizing soft tissue in the mammalian cochlea with coherent hard X-rays Rau, C.; Robinson, I. K.; Richter, C. -P. Microscopy Research and Technique, Vol. 69, Issue 8 https://doi.org/10.1002/jemt.20336	journal	January 2006
Imaging of cochlear tissue with a grating interferometer and hard X-rays Richter, Claus-Peter; Shintani-Smith, Stephanie; Fishman, Andrew Microscopy Research and Technique, Vol. 72, Issue 12 https://doi.org/10.1002/jemt.20728	journal	December 2009
Safety of Adeno-Associated Virus as Cochlear Gene Transfer Vector: Analysis of Distant Spread Beyond Injected Cochleae Kho, Soochuen T.; Pettis, Robert M.; Mhatre, Anand N. Molecular Therapy, Vol. 2, Issue 4 https://doi.org/10.1006/mthe.2000.0129	journal	October 2000
Genetics of hearing loss: where are we standing now? Mahboubi, Hossein; Dwabe, Sami; Fradkin, Matthew European Archives of Oto-Rhino-Laryngology, Vol. 269, Issue 7 https://doi.org/10.1007/s00405-011-1910-6	journal	January 2012
Profound hearing loss in the cat following the single co-administration of kanamycin and ethacrynic acid Xu, Shi-Ang; Shepherd, Robert K.; Chen, Yin Hearing Research, Vol. 70, Issue 2 https://doi.org/10.1016/0378-5955(93)90159-x	journal	November 1993
How the ear's works work: mechanoelectrical transduction and amplification by hair cells Hudspeth, A. J. Comptes Rendus Biologies, Vol. 328, Issue 2 https://doi.org/10.1016/j.crvi.2004.12.003	journal	February 2005
Death pathways in noise-damaged outer hair cells Bohne, Barbara A.; Harding, Gary W.; Lee, Steve C. Hearing Research, Vol. 223, Issue 1-2 https://doi.org/10.1016/j.heares.2006.10.004	journal	January 2007
Gentamicin uptake in the chinchilla inner ear Roehm, Pamela; Hoffer, Michael; Balaban, Carey D. Hearing Research, Vol. 230, Issue 1-2 https://doi.org/10.1016/j.heares.2007.04.005	journal	August 2007
Contribution of genetic factors to noise-induced hearing loss: A human studies review Sliwinska-Kowalska, Mariola; Pawelczyk, Malgorzata Mutation Research/Reviews in Mutation Research, Vol. 752, Issue 1 https://doi.org/10.1016/j.mrrev.2012.11.001	journal	January 2013
Atorvastatin slows down the deterioration of inner ear function with age in mice Syka, Josef; Ouda, Ladislav; Nachtigal, Petr Neuroscience Letters, Vol. 411, Issue 2 https://doi.org/10.1016/j.neulet.2006.10.032	journal	January 2007
Pravastatin attenuates noise-induced cochlear injury in mice Park, J. S.; Kim, S. W.; Park, K. Neuroscience, Vol. 208 https://doi.org/10.1016/j.neuroscience.2012.02.010	journal	April 2012
Sound advice for public health The Lancet, Vol. 383, Issue 9925 https://doi.org/10.1016/s0140-6736(14)60631-0	journal	April 2014
Electrokinetic shape changes of cochlear outer hair cells Kachar, Bechara; Brownell, William E.; Altschuler, Richard Nature, Vol. 322, Issue 6077 https://doi.org/10.1038/322365a0	journal	July 1986
How the ear's works work Hudspeth, A. J. Nature, Vol. 341, Issue 6241 https://doi.org/10.1038/341397a0	journal	October 1989
Is the din really harmless? Long-term effects of non-traumatic noise on the adult auditory system Gourévitch, Boris; Edeline, Jean-Marc; Occelli, Florian Nature Reviews Neuroscience, Vol. 15, Issue 7 https://doi.org/10.1038/nrn3744	journal	June 2014
Transduction of the contralateral ear after adenovirus-mediated cochlear gene transfer Stöver, T.; Yagi, M.; Raphael, Y. Gene Therapy, Vol. 7, Issue 5 https://doi.org/10.1038/sj.gt.3301108	journal	February 2000
Novel High Content Screen Detects Compounds That Promote Neurite Regeneration from Cochlear Spiral Ganglion Neurons Whitlon, Donna S.; Grover, Mary; Dunne, Sara F. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep15960	journal	November 2015
Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object Paganin, D.; Mayo, S. C.; Gureyev, T. E. Journal of Microscopy, Vol. 206, Issue 1 https://doi.org/10.1046/j.1365-2818.2002.01010.x	journal	April 2002
Possible role of HMG-CoA reductase inhibitors for the treatment of sudden sensorineural hearing loss (SSHL) Borghi, C.; Modugno, G. C.; Pirodda, A. Medical Hypotheses, Vol. 58, Issue 5 https://doi.org/10.1054/mehy.2001.1535	journal	May 2002
Genetic diseases of hearing Spillmann, Thomas Current Opinion in Neurology, Vol. 7, Issue 1 https://doi.org/10.1097/00019052-199402000-00015	journal	January 1994
Genetic Studies on Noise-Induced Hearing Loss: A Review Konings, Annelies; Laer, Lut Van; Camp, Guy Van Ear and Hearing, Vol. 30, Issue 2 https://doi.org/10.1097/aud.0b013e3181987080	journal	January 2009
Drug Delivery for Treatment of Inner Ear Disease: Current State of Knowledge McCall, Andrew A.; Swan, Erin E. Leary; Borenstein, Jeffrey T. Ear and Hearing, Vol. 31, Issue 2 https://doi.org/10.1097/aud.0b013e3181c351f2	journal	January 2010
Cellular mechanisms of aminoglycoside ototoxicity Warchol, Mark E. Current Opinion in Otolaryngology & Head and Neck Surgery, Vol. 18, Issue 5 https://doi.org/10.1097/moo.0b013e32833e05ec	journal	January 2010
High-resolution X-ray tomography of the human inner ear: synchrotron radiation-based study of nerve fibre bundles, membranes and ganglion cells Lareida, A.; Beckmann, F.; Schrott-Fischer, A. Journal of Microscopy, Vol. 234, Issue 1 https://doi.org/10.1111/j.1365-2818.2009.03143.x	journal	April 2009
Sex-linked deafness Petersen, Mb; Wang, Q.; Willems, Pj Clinical Genetics, Vol. 73, Issue 1 https://doi.org/10.1111/j.1399-0004.2007.00913.x	journal	November 2007
Acoustic injury and the physiology of hearing Schmiedt, Richard A. The Journal of the Acoustical Society of America, Vol. 76, Issue 5 https://doi.org/10.1121/1.391446	journal	November 1984
The anatomical consequences of acoustic injury: A review and tutorial Saunders, James C.; Dear, Steven P.; Schneider, Mark E. The Journal of the Acoustical Society of America, Vol. 78, Issue 3 https://doi.org/10.1121/1.392915	journal	September 1985
The structural and functional consequences of acoustic injury in the cochlea and peripheral auditory system: A five year update Saunders, James C.; Cohen, Yale E.; Szymko, Yvonne M. The Journal of the Acoustical Society of America, Vol. 90, Issue 1 https://doi.org/10.1121/1.401307	journal	July 1991
Evoked mechanical responses of isolated cochlear outer hair cells Brownell, W.; Bader, C.; Bertrand, D. Science, Vol. 227, Issue 4683 https://doi.org/10.1126/science.3966153	journal	January 1985
Genetic investigations in childhood deafness Parker, Michael; Bitner-Glindzicz, Maria Archives of Disease in Childhood, Vol. 100, Issue 3 https://doi.org/10.1136/archdischild-2014-306099	journal	October 2014
Properties of auditory nerve responses in absence of outer hair cells Dallos, P.; Harris, D. Journal of Neurophysiology, Vol. 41, Issue 2 https://doi.org/10.1152/jn.1978.41.2.365	journal	March 1978
Identifying components of the hair-cell interactome involved in cochlear amplification Zheng, Jing; Anderson, Charles T.; Miller, Katharine K. BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-127	journal	January 2009
Functional and morphological changes in the cochlea of cholesterolfed guinea pigs. [高脂食負荷モルモット内耳か牛の機能的，形態学的変化] Kashiwako, Izumi; Hattori, Yasuo; Qiao, Yan Journal of Nippon Medical School, Vol. 61, Issue 4 https://doi.org/10.1272/jnms1923.61.321	journal	January 1994
Early investigational drugs for hearing loss Mukherjea, Debashree; Ghosh, Sumana; Bhatta, Puspanjali Expert Opinion on Investigational Drugs, Vol. 24, Issue 2 https://doi.org/10.1517/13543784.2015.960076	journal	September 2014
Emerging treatments for noise-induced hearing loss Oishi, Naoki; Schacht, Jochen Expert Opinion on Emerging Drugs, Vol. 16, Issue 2 https://doi.org/10.1517/14728214.2011.552427	journal	January 2011
Comparative Adverse Effect Profiles of Platinum Drugs McKeage, Mark J. Drug Safety, Vol. 13, Issue 4 https://doi.org/10.2165/00002018-199513040-00003	journal	January 1995
A Mechano-Electrical Theory of Cochlear Action Davis, Hallowell Acta Oto-Laryngologica, Vol. 50, Issue 1-2 https://doi.org/10.3109/00016485909129164	journal	January 1959
Structural Changes in the Cochlea Following Overstimulation by Noise Engström, H.; Engström, B. Acta Oto-Laryngologica, Vol. 86, Issue sup360 https://doi.org/10.3109/00016487809123479	journal	January 1978
Time Sequence of Degeneration Pattern of the Organ of Corti after Acoustic Overstimulation: A Transmission Electron Microscopy Study Fredelius, Lars Acta Oto-Laryngologica, Vol. 106, Issue 5-6 https://doi.org/10.3109/00016488809122260	journal	January 1988
Direct MicroCT imaging of non-mineralized connective tissues at high resolution Naveh, Gili R. S.; Brumfeld, Vlad; Dean, Mason Connective Tissue Research, Vol. 55, Issue 1 https://doi.org/10.3109/03008207.2013.867333	journal	January 2014
Music exposure and hearing disorders: An overview Zhao, Fei; Manchaiah, Vinaya K. C.; French, David International Journal of Audiology, Vol. 49, Issue 1 https://doi.org/10.3109/14992020903202520	journal	December 2009
Renexin as a rescue regimen for noise-induced hearing loss Park, ShiNae; Yeo, SangWon; Back, SangA Noise and Health, Vol. 16, Issue 72 https://doi.org/10.4103/1463-1741.140497	journal	January 2014

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