Title: Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging

Abstract

Diffraction before destruction using X-ray free-electron lasers (XFELs) has the potential to determine radiation-damage-free structures without the need for crystallization. This article presents the three-dimensional reconstruction of the Melbournevirus from single-particle X-ray diffraction patterns collected at the LINAC Coherent Light Source (LCLS) as well as reconstructions from simulated data exploring the consequences of different kinds of experimental sources of noise. The reconstruction from experimental data suffers from a strong artifact in the center of the particle. This could be reproduced with simulated data by adding experimental background to the diffraction patterns. In those simulations, the relative density of the artifact increases linearly with background strength. This suggests that the artifact originates from the Fourier transform of the relatively flat background, concentrating all power in a central feature of limited extent. We support these findings by significantly reducing the artifact through background removal before the phase-retrieval step. Large amounts of blurring in the diffraction patterns were also found to introduce diffuse artifacts, which could easily be mistaken as biologically relevant features. Other sources of noise such as sample heterogeneity and variation of pulse energy did not significantly degrade the quality of the reconstructions. Larger data volumes, made possible by the recentmore » inauguration of high repetition-rate XFELs, allow for increased signal-to-background ratio and provide a way to minimize these artifacts. The anticipated development of three-dimensional Fourier-volume-assembly algorithms which are background aware is an alternative and complementary solution, which maximizes the use of data.« less

Authors:

Lundholm, Ida V.; Sellberg, Jonas A.; Ekeberg, Tomas; Hantke, Max F.; Okamoto, Kenta; van der Schot, Gijs; Andreasson, Jakob; Barty, Anton; Bielecki, Johan; Bruza, Petr; Bucher, Max; Carron, Sebastian; Daurer, Benedikt J.; Ferguson, Ken; Hasse, Dirk; Krzywinski, Jacek; Larsson, Daniel S. D.; Morgan, Andrew; Mühlig, Kerstin; Müller, Maria more »; Nettelblad, Carl; Pietrini, Alberto; Reddy, Hemanth K. N.; Rupp, Daniela; Sauppe, Mario; Seibert, Marvin; Svenda, Martin; Swiggers, Michelle; Timneanu, Nicusor; Ulmer, Anatoli; Westphal, Daniel; Williams, Garth; Zani, Alessandro; Faigel, Gyula; Chapman, Henry N.; Möller, Thomas; Bostedt, Christoph; Hajdu, Janos; Gorkhover, Tais; Maia, Filipe R. N. C. « less

Publication Date:

Sat Sep 01 00:00:00 EDT 2018

Research Org.:

SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:

1468673

Alternate Identifier(s):

OSTI ID: 1475539; OSTI ID: 1477402; OSTI ID: 1480964

Report Number(s):

BNL-209371-2018-JAAM
Journal ID: ISSN 2052-2525; IUCRAJ; PII: S2052252518010047

Grant/Contract Number:  

K115504; AC02-76SF00515; AC02-05CH11231; SC0012704

Resource Type:

Published Article

Journal Name:

IUCrJ

Additional Journal Information:

Journal Name: IUCrJ Journal Volume: 5 Journal Issue: 5; Journal ID: ISSN 2052-2525

Publisher:

International Union of Crystallography (IUCr)

Country of Publication:

United Kingdom

Language:

English

Subject:

47 OTHER INSTRUMENTATION; XFELs; Melbournevirus; coherent diffractive imaging; LCLS; image reconstruction; 59 BASIC BIOLOGICAL SCIENCES