Response of bentonite microbial communities to stresses relevant to geodisposal of radioactive waste

doi:10.1016/j.chemgeo.2018.10.004

Title: Response of bentonite microbial communities to stresses relevant to geodisposal of radioactive waste

Abstract

Microbes have been isolated previously from bentonite materials that may be used as barriers for the disposal of radioactive waste. Actively respiring microbes in such barrier materials, within a repository environment, have the potential to adversely affect waste container corrosion rates. Additionally, they could potentially alter the properties of the bentonite barrier itself. This is of significance, since the integrity of the waste container and properties of the bentonite barrier are required to fulfil defined safety functions. Furthermore to help identify the critical factors that affect microbial activity in bentonite materials, this study examines the impact of a range of parameters that could affect microbial metabolism in a geodisposal environment. Several bentonites from different sources (bentonite mined from locations in Spain and the USA, along with commercially-sourced bentonite) were subjected to increased pressure (74 MPa, 30 s), heat (90 °C, 24 h), and irradiation (1000 Gy, 24.17 Gy min ^–1), before incubation in growth media selective for sulfate-reducing bacteria (SRB) or iron-reducing bacteria (IRB).

Authors:

^[1]; Pearce, Carolyn I. ^[2]; Boothman, Chris ^[1]; Lloyd, Jonathan R. ^[1]

Univ. of Manchester, Manchester (United Kingdom)
Univ. of Manchester, Manchester (United Kingdom); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Publication Date:: 2018-10-07

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1480131

Report Number(s):: [PNNL-SA-139606]
[Journal ID: ISSN 0009-2541; PII: S0009254118304996]

Grant/Contract Number:: [861880; NE/H007768/1L; AC05-76RL01830]

Resource Type:: Accepted Manuscript

Journal Name:: Chemical Geology

Additional Journal Information:: [ Journal Volume: 501]; Journal ID: ISSN 0009-2541

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 58 GEOSCIENCES; Bentonite; Sulfate-reducing bacteria; Fe(III)-reducing bacteria; Geological disposal facility

Citation Formats


                    Haynes, Haydn M., Pearce, Carolyn I., Boothman, Chris, and Lloyd, Jonathan R. Response of bentonite microbial communities to stresses relevant to geodisposal of radioactive waste.  United States: N. p., 2018. 
        Web.  doi:10.1016/j.chemgeo.2018.10.004.

Copy to clipboard


                    Haynes, Haydn M., Pearce, Carolyn I., Boothman, Chris, & Lloyd, Jonathan R. Response of bentonite microbial communities to stresses relevant to geodisposal of radioactive waste.  United States.  doi:10.1016/j.chemgeo.2018.10.004.

Copy to clipboard


                    Haynes, Haydn M., Pearce, Carolyn I., Boothman, Chris, and Lloyd, Jonathan R. Sun .  
        "Response of bentonite microbial communities to stresses relevant to geodisposal of radioactive waste".  United States.  doi:10.1016/j.chemgeo.2018.10.004.  https://www.osti.gov/servlets/purl/1480131.

Copy to clipboard


                    
@article{osti_1480131,

  title        = {Response of bentonite microbial communities to stresses relevant to geodisposal of radioactive waste},

  author       = {Haynes, Haydn M. and Pearce, Carolyn I. and Boothman, Chris and Lloyd, Jonathan R.},

  abstractNote = {Microbes have been isolated previously from bentonite materials that may be used as barriers for the disposal of radioactive waste. Actively respiring microbes in such barrier materials, within a repository environment, have the potential to adversely affect waste container corrosion rates. Additionally, they could potentially alter the properties of the bentonite barrier itself. This is of significance, since the integrity of the waste container and properties of the bentonite barrier are required to fulfil defined safety functions. Furthermore to help identify the critical factors that affect microbial activity in bentonite materials, this study examines the impact of a range of parameters that could affect microbial metabolism in a geodisposal environment. Several bentonites from different sources (bentonite mined from locations in Spain and the USA, along with commercially-sourced bentonite) were subjected to increased pressure (74 MPa, 30 s), heat (90 °C, 24 h), and irradiation (1000 Gy, 24.17 Gy min–1), before incubation in growth media selective for sulfate-reducing bacteria (SRB) or iron-reducing bacteria (IRB).},

  doi          = {10.1016/j.chemgeo.2018.10.004},

  journal      = {Chemical Geology},

  number       = ,

  volume       = [501],

  place        = {United States},

  year         = {2018},

  month        = {10}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

DOI: 10.1016/j.chemgeo.2018.10.004

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 1 work

Citation information provided by
Web of Science

Figures / Tables:

Table 1: Key chemical characteristics, including the water content, loss on ignition (LOI), pH, surface area (SA), concentration of key interlayer cations (Na⁺, Ca²⁺, K⁺, Mg²⁺), along with the concentration of SO₄²⁻, the redox state of 0.5M HCl extractable Fe, and the mineralogy.

All figures and tables (6 total)

Save / Share:

Export Metadata

Save to My Library

Figures / Tables found in this record:

Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

Similar Records in DOE PAGES and OSTI.GOV collections:

On the long-term migration of uranyl in bentonite barrier for high-level radioactive waste repositories: The effect of different host rocks

Journal Article Cao, Xiaoyuan ; Zheng, Liange ; Hou, Deyi ; ... - Chemical Geology

Repositories for high level radioactive waste hosted in argillite or granite with bentonite backfill are the two disposal concepts that been extensively studied. A general perception is that granite has very low sorption capacity and therefore is disadvantageous in retarding the migration of radionuclides in comparison with argillite. However, when there is bentonite backfill, does the sorption capacity of granite really matter for the migration of radionuclides? How different host rocks would affect the migration of U(VI) through the bentonite backfill of an engineered barrier system (EBS), and which properties of the host rocks cause those differences in migration, givenmore »« less
DOI: 10.1016/j.chemgeo.2019.07.006
Fe-saponite growth on low-carbon and stainless steel in hydrothermal-bentonite experiments

Journal Article Cheshire, Michael C. ; A. Caporuscio, Florie ; Jove-Colon, Carlos F. ; ... - Journal of Nuclear Materials

Hydrothermal experiments on engineered barrier system (EBS) materials were conducted to characterize high temperature interactions between bentonite and candidate waste container steels (304SS, 316SS, low-C steel) for deep geological disposition of nuclear spent fuel. In this study, hydrothermal experiments were performed using Dickson reaction cells at temperatures and pressure of up to 300 °C and 15–16 MPa, respectively, for four to six weeks. Wyoming bentonite was saturated with a 1900 ppm K-Ca-Na-Cl solution in combination with stainless and low-C steel coupons. Authigenic Fe-saponite precipitated utilizing steel as a growth substrate with Fe being supplied by steel corrosion. Concurrent with Fe-saponitemore »« less
DOI: 10.1016/j.jnucmat.2018.09.038

Full Text Available
Fermentation couples Chloroflexi and sulfate-reducing bacteria to Cyanobacteria in hypersaline microbial mats

Journal Article Lee, Jackson Z. ; Burow, Luke C. ; Woebken, Dagmar ; ... - Frontiers in Microbiology

Past studies of hydrogen cycling in hypersaline microbial mats have shown an active nighttime cycle, with production largely from Cyanobacteria and consumption from sulfate-reducing bacteria (SRB). However, the mechanisms and magnitude of hydrogen cycling have not been extensively studied. Two mats types near Guerrero Negro, Mexico$-$ permanently submerged Microcoleus microbial mat (GN-S), and intertidal Lyngbya microbial mat (GN-I)$-$were used in microcosm diel manipulation experiments with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), molybdate, ammonium addition, and physical disruption to understand the processes responsible for hydrogen cycling between mat microbes. Across microcosms, H ₂ production occurred under dark anoxic conditions with simultaneous production of a suitemore »« less
Cited by 33
DOI: 10.3389/fmicb.2014.00061

Full Text Available
Microbial Ecology on Solar Panels in Berkeley, CA, United States

Journal Article Porcar, Manuel ; Louie, Katherine B. ; Kosina, Suzanne M. ; ... - Frontiers in Microbiology

Solar panels can be found practically all over the world and represent a standard surface that can be colonized by microbial communities that are resistant to harsh environmental conditions, including high irradiation, temperature fluctuations and desiccation. These properties make them not only ideal sources of stress-resistant bacteria, but also standard devices to study the microbial communities and their colonization process from different areas of Earth. We report here a comprehensive description of the microbial communities associated with solar panels in Berkeley, CA, United States. Cultivable bacteria were isolated to characterize their adhesive capabilities, and UV- and desiccation-resistance properties. Furthermore, amore »« less
Cited by 1
DOI: 10.3389/fmicb.2018.03043

Full Text Available
Web of microbes (WoM): a curated microbial exometabolomics database for linking chemistry and microbes

Journal Article Kosina, Suzanne M. ; Greiner, Annette M. ; Lau, Rebecca K. ; ... - BMC Microbiology

Background: As microbiome research becomes increasingly prevalent in the fields of human health, agriculture and biotechnology, there exists a need for a resource to better link organisms and environmental chemistries. Exometabolomics experiments now provide assertions of the metabolites present within specific environments and how the production and depletion of metabolites is linked to specific microbes. This information could be broadly useful, from comparing metabolites across environments, to predicting competition and exchange of metabolites between microbes, and to designing stable microbial consortia. Here, we introduce Web of Microbes (WoM; freely available at: http://webofmicrobes.org), the first exometabolomics data repository and visualization tool.more »« less
Cited by 1
DOI: 10.1186/s12866-018-1256-y

Full Text Available

Similar Records
Related Works
dataset (1)