Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

Hovde, Blake T.; Hanschen, Erik R.; Steadman Tyler, Christina R.; Lo, Chien-Chi; Kunde, Yuliya; Davenport, Karen; Daligault, Hajnalka; Msanne, Joseph; Canny, Stephanie; Eyun, Seong-il; Riethoven, Jean-Jack M.; Polle, Juergen; Starkenburg, Shawn R.

doi:10.1016/j.algal.2018.09.012

Title: Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

Journal Article · 30 September 2018 · Algal Research

DOI: https://doi.org/10.1016/j.algal.2018.09.012 · OSTI ID:1479995

Hovde, Blake T. ^[1]; Hanschen, Erik R. ^[1]; Steadman Tyler, Christina R. ^[1]; Lo, Chien-Chi ^[1]; Kunde, Yuliya ^[1]; Davenport, Karen ^[1]; Daligault, Hajnalka ^[1];

^[2]; Canny, Stephanie ^[3];

^[4]; Riethoven, Jean-Jack M. ^[5]; Polle, Juergen ^[6]; Starkenburg, Shawn R. ^[1]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Univ. of Nebraska, Lincoln, NE (United States). Center for Plant Science Innovation. School of Natural Resources
Univ. of Nebraska, Lincoln, NE (United States). Center for Biotechnology
Chung-Ang Univ., Seoul (Korea, Republic of). Dept. of Life Science
Univ. of Nebraska, Lincoln, NE (United States). Center for Biotechnology. School of Biological Sciences
CUNY Brooklyn College, New York, NY (United States)

Selection of highly productive algal strains is crucial for establishing economically viable biomass and bioproduct cultivation systems. Characterization of algal genomes, including understanding strain-specific differences in genome content and architecture is a critical step in this process. Using genomic analyses, we demonstrate significant differences between three strains of Chlorella sorokiniana (strain 1228, UTEX 1230, and DOE1412). We found that unique, strain-specific genes comprise a substantial proportion of each genome, and genomic regions with >80% local nucleotide identity constitute <15% of each genome among the strains, indicating substantial strain specific evolution. Furthermore, cataloging of meiosis and other sex-related genes in C. sorokiniana strains suggests strategic breeding could be utilized to improve biomass and bioproduct yields if a sexual cycle can be characterized. Finally, preliminary investigation of epigenetic machinery suggests the presence of potentially unique transcriptional regulation in each strain. Our data demonstrate that these three C. sorokiniana strains represent significantly different genomic content. Based on these findings, we propose individualized assessment of each strain for potential performance in cultivation systems.

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Research Organization:: Los Alamos National Laboratory (LANL)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1479995

Report Number(s):: LA-UR-18-24143

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 35; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (75)

Discovery of a New Bacterial Polyketide Biosynthetic Pathway Moore, Bradley S.; Hopke, Jörn N. ChemBioChem, Vol. 2, Issue 1 https://doi.org/10.1002/1439-7633(20010105)2:1<35::AID-CBIC35>3.0.CO;2-1	journal	January 2001
Animal evolution during domestication: the domesticated fox as a model Trut, Lyudmila; Oskina, Irina; Kharlamova, Anastasiya BioEssays, Vol. 31, Issue 3 https://doi.org/10.1002/bies.200800070	journal	March 2009
Turnover of Mature miRNAs and siRNAs in Plants and Algae Cerutti, Heriberto; Ibrahim, Fadia Advances in Experimental Medicine and Biology https://doi.org/10.1007/978-1-4419-7823-3_11	book	January 2010
Bacterial type III polyketide synthases: phylogenetic analysis and potential for the production of novel secondary metabolites by heterologous expression in pseudomonads Gross, Frank; Luniak, Nora; Perlova, Olena Archives of Microbiology, Vol. 185, Issue 1 https://doi.org/10.1007/s00203-005-0059-3	journal	January 2006
Climate-simulated raceway pond culturing: quantifying the maximum achievable annual biomass productivity of Chlorella sorokiniana in the contiguous USA Huesemann, M.; Chavis, A.; Edmundson, S. Journal of Applied Phycology, Vol. 30, Issue 1 https://doi.org/10.1007/s10811-017-1256-6	journal	September 2017
The relation of recombination to mutational advance Muller, H. J. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 1, Issue 1 https://doi.org/10.1016/0027-5107(64)90047-8	journal	May 1964
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Yields of cultured Pacific oysters Crassostrea gigas Thunberg improved after one generation of selection Langdon, Chris; Evans, Ford; Jacobson, Dave Aquaculture, Vol. 220, Issue 1-4 https://doi.org/10.1016/S0044-8486(02)00621-X	journal	April 2003
Polyketide biosynthesis beyond the type I, II and III polyketide synthase paradigms Shen, B. Current Opinion in Chemical Biology, Vol. 7, Issue 2 https://doi.org/10.1016/S1367-5931(03)00020-6	journal	April 2003
A validated model to predict microalgae growth in outdoor pond cultures subjected to fluctuating light intensities and water temperatures Huesemann, M.; Crowe, B.; Waller, P. Algal Research, Vol. 13 https://doi.org/10.1016/j.algal.2015.11.008	journal	January 2016
Characterization and classification of highly productive microalgae strains discovered for biofuel and bioproduct generation Neofotis, Peter; Huang, Andy; Sury, Kiran Algal Research, Vol. 15 https://doi.org/10.1016/j.algal.2016.01.007	journal	April 2016
Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts Unkefer, Clifford J.; Sayre, Richard T.; Magnuson, Jon K. Algal Research, Vol. 22 https://doi.org/10.1016/j.algal.2016.06.002	journal	March 2017
Simulation of outdoor pond cultures using indoor LED-lighted and temperature-controlled raceway ponds and Phenometrics photobioreactors Huesemann, M.; Dale, T.; Chavis, A. Algal Research, Vol. 21 https://doi.org/10.1016/j.algal.2016.11.016	journal	January 2017
Review of the cultivation program within the National Alliance for Advanced Biofuels and Bioproducts Lammers, Peter J.; Huesemann, Michael; Boeing, Wiebke Algal Research, Vol. 22 https://doi.org/10.1016/j.algal.2016.11.021	journal	March 2017
Excision of 5-hydroxymethylcytosine by DEMETER family DNA glycosylases Jang, Hosung; Shin, Hosub; Eichman, Brandt F. Biochemical and Biophysical Research Communications, Vol. 446, Issue 4 https://doi.org/10.1016/j.bbrc.2014.03.060	journal	April 2014
Gene silencing in microalgae: Mechanisms and biological roles Kim, Eun-Jeong; Ma, Xinrong; Cerutti, Heriberto Bioresource Technology, Vol. 184 https://doi.org/10.1016/j.biortech.2014.10.119	journal	May 2015
Role of the DRM and CMT3 Methyltransferases in RNA-Directed DNA Methylation Cao, Xiaofeng; Aufsatz, Werner; Zilberman, Daniel Current Biology, Vol. 13, Issue 24 https://doi.org/10.1016/j.cub.2003.11.052	journal	December 2003
Causes and effects of nuclear genome reduction Keeling, Patrick J.; Slamovits, Claudio H. Current Opinion in Genetics & Development, Vol. 15, Issue 6 https://doi.org/10.1016/j.gde.2005.09.003	journal	December 2005
Novel Insights into Evolution of Protistan Polyketide Synthases through Phylogenomic Analysis John, Uwe; Beszteri, Bánk; Derelle, Evelyne Protist, Vol. 159, Issue 1 https://doi.org/10.1016/j.protis.2007.08.001	journal	January 2008
Sex determination in Chlamydomonas Goodenough, Ursula; Lin, Huawen; Lee, Jae-Hyeok Seminars in Cell & Developmental Biology, Vol. 18, Issue 3 https://doi.org/10.1016/j.semcdb.2007.02.006	journal	June 2007
Full-length transcriptome assembly from RNA-Seq data without a reference genome Grabherr, Manfred G.; Haas, Brian J.; Yassour, Moran Nature Biotechnology, Vol. 29, Issue 7 https://doi.org/10.1038/nbt.1883	journal	May 2011
Experimental evolution of an alternating uni- and multicellular life cycle in Chlamydomonas reinhardtii Ratcliff, William C.; Herron, Matthew D.; Howell, Kathryn Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms3742	journal	November 2013
Fast gapped-read alignment with Bowtie 2 Langmead, Ben; Salzberg, Steven L. Nature Methods, Vol. 9, Issue 4 https://doi.org/10.1038/nmeth.1923	journal	March 2012
Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data Chin, Chen-Shan; Alexander, David H.; Marks, Patrick Nature Methods, Vol. 10, Issue 6 https://doi.org/10.1038/nmeth.2474	journal	May 2013
De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis Haas, Brian J.; Papanicolaou, Alexie; Yassour, Moran Nature Protocols, Vol. 8, Issue 8 https://doi.org/10.1038/nprot.2013.084	journal	July 2013
RNA-directed DNA methylation: an epigenetic pathway of increasing complexity Matzke, Marjori A.; Mosher, Rebecca A. Nature Reviews Genetics, Vol. 15, Issue 6 https://doi.org/10.1038/nrg3683	journal	May 2014
Polyketide biosynthesis: a millennium review Staunton, James; Weissman, Kira J. Natural Product Reports, Vol. 18, Issue 4 https://doi.org/10.1039/a909079g	journal	January 2001
Diversity, taxonomy and evolution of medium-chain dehydrogenase/reductase superfamily Riveros-Rosas, Hector; Julian-Sanchez, Adriana; Villalobos-Molina, Rafael European Journal of Biochemistry, Vol. 270, Issue 16 https://doi.org/10.1046/j.1432-1033.2003.03704.x	journal	August 2003
The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation Palenik, B.; Grimwood, J.; Aerts, A. Proceedings of the National Academy of Sciences, Vol. 104, Issue 18 https://doi.org/10.1073/pnas.0611046104	journal	April 2007
Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth Trentacoste, E. M.; Shrestha, R. P.; Smith, S. R. Proceedings of the National Academy of Sciences, Vol. 110, Issue 49 https://doi.org/10.1073/pnas.1309299110	journal	November 2013
Chromosome-level genome assembly and transcriptome of the green alga Chromochloris zofingiensis illuminates astaxanthin production Roth, Melissa S.; Cokus, Shawn J.; Gallaher, Sean D. Proceedings of the National Academy of Sciences, Vol. 114, Issue 21 https://doi.org/10.1073/pnas.1619928114	journal	May 2017
The GreenCut2 Resource, a Phylogenomically Derived Inventory of Proteins Specific to the Plant Lineage Karpowicz, Steven J.; Prochnik, Simon E.; Grossman, Arthur R. Journal of Biological Chemistry, Vol. 286, Issue 24 https://doi.org/10.1074/jbc.M111.233734	journal	April 2011
Profile hidden Markov models Eddy, S. R. Bioinformatics, Vol. 14, Issue 9 https://doi.org/10.1093/bioinformatics/14.9.755	journal	October 1998
InterProScan 5: genome-scale protein function classification Jones, P.; Binns, D.; Chang, H. -Y. Bioinformatics, Vol. 30, Issue 9 https://doi.org/10.1093/bioinformatics/btu031	journal	January 2014
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies Stamatakis, Alexandros Bioinformatics, Vol. 30, Issue 9 https://doi.org/10.1093/bioinformatics/btu033	journal	January 2014
BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs Simão, Felipe A.; Waterhouse, Robert M.; Ioannidis, Panagiotis Bioinformatics, Vol. 31, Issue 19 https://doi.org/10.1093/bioinformatics/btv351	journal	June 2015
Chromosome Scale Genome Assembly and Transcriptome Profiling of Nannochloropsis gaditana in Nitrogen Depletion Corteggiani Carpinelli, Elisa; Telatin, Andrea; Vitulo, Nicola Molecular Plant, Vol. 7, Issue 2 https://doi.org/10.1093/mp/sst120	journal	February 2014
A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri Babinger, P. Nucleic Acids Research, Vol. 29, Issue 6 https://doi.org/10.1093/nar/29.6.1261	journal	March 2001
MUSCLE: multiple sequence alignment with high accuracy and high throughput Edgar, R. C. Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797 https://doi.org/10.1093/nar/gkh340	journal	March 2004
AUGUSTUS: a web server for gene prediction in eukaryotes that allows user-defined constraints Stanke, M.; Morgenstern, B. Nucleic Acids Research, Vol. 33, Issue Web Server https://doi.org/10.1093/nar/gki458	journal	July 2005
High-throughput functional annotation and data mining with the Blast2GO suite Gotz, S.; Garcia-Gomez, J. M.; Terol, J. Nucleic Acids Research, Vol. 36, Issue 10 https://doi.org/10.1093/nar/gkn176	journal	April 2008
Gene3D: a domain-based resource for comparative genomics, functional annotation and protein network analysis Lees, J.; Yeats, C.; Perkins, J. Nucleic Acids Research, Vol. 40, Issue D1 https://doi.org/10.1093/nar/gkr1181	journal	December 2011
Pfam: the protein families database Finn, Robert D.; Bateman, Alex; Clements, Jody Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1223	journal	November 2013
Enabling the democratization of the genomics revolution with a fully integrated web-based bioinformatics platform Li, Po-E; Lo, Chien-Chi; Anderson, Joseph J. Nucleic Acids Research, Vol. 45, Issue 1 https://doi.org/10.1093/nar/gkw1027	journal	November 2016
PANTHER version 11: expanded annotation data from Gene Ontology and Reactome pathways, and data analysis tool enhancements Mi, Huaiyu; Huang, Xiaosong; Muruganujan, Anushya Nucleic Acids Research, Vol. 45, Issue D1 https://doi.org/10.1093/nar/gkw1138	journal	November 2016
MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space Ronquist, Fredrik; Teslenko, Maxim; van der Mark, Paul Systematic Biology, Vol. 61, Issue 3 https://doi.org/10.1093/sysbio/sys029	journal	February 2012
Mechanisms and functions of Tet protein-mediated 5-methylcytosine oxidation Wu, H.; Zhang, Y. Genes & Development, Vol. 25, Issue 23 https://doi.org/10.1101/gad.179184.111	journal	December 2011
Velvet: Algorithms for de novo short read assembly using de Bruijn graphs Zerbino, D. R.; Birney, E. Genome Research, Vol. 18, Issue 5 https://doi.org/10.1101/gr.074492.107	journal	February 2008
Base-Calling of Automated Sequencer Traces Using Phred. I. Accuracy Assessment Ewing, Brent; Hillier, LaDeana; Wendl, Michael C. Genome Research, Vol. 8, Issue 3 https://doi.org/10.1101/gr.8.3.175	journal	March 1998
Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities Ewing, Brent; Green, Phil Genome Research, Vol. 8, Issue 3 https://doi.org/10.1101/gr.8.3.186	journal	March 1998
Consed: A Graphical Tool for Sequence Finishing Gordon, David; Abajian, Chris; Green, Phil Genome Research, Vol. 8, Issue 3 https://doi.org/10.1101/gr.8.3.195	journal	March 1998
The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex Blanc, Guillaume; Duncan, Garry; Agarkova, Irina The Plant Cell, Vol. 22, Issue 9 https://doi.org/10.1105/tpc.110.076406	journal	September 2010
Dna Nucleoside Composition and Methylation in Several Species of Microalgae1 Jarvis, Eric E.; Dunahay, Terri G.; Brown, Lewis M. Journal of Phycology, Vol. 28, Issue 3 https://doi.org/10.1111/j.0022-3646.1992.00356.x	journal	June 1992
Meiotic genes and sexual reproduction in the green algal class Trebouxiophyceae (Chlorophyta) Fučíková, Karolina; Pažoutová, Marie; Rindi, Fabio Journal of Phycology, Vol. 51, Issue 3 https://doi.org/10.1111/jpy.12293	journal	April 2015
The Chlamydomonas cell cycle Cross, Frederick R.; Umen, James G. The Plant Journal, Vol. 82, Issue 3 https://doi.org/10.1111/tpj.12795	journal	March 2015
Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga Arriola, Matthew B.; Velmurugan, Natarajan; Zhang, Ying The Plant Journal, Vol. 93, Issue 3 https://doi.org/10.1111/tpj.13789	journal	January 2018
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions Merchant, S. S.; Prochnik, S. E.; Vallon, O. Science, Vol. 318, Issue 5848, p. 245-250 https://doi.org/10.1126/science.1143609	journal	October 2007
Real-Time DNA Sequencing from Single Polymerase Molecules Eid, John; Fehr, Adrian; Gray, Jeremy Science, Vol. 323, Issue 5910, p. 133-138 https://doi.org/10.1126/science.1162986	journal	January 2009
Genomic Analysis of Organismal Complexity in the Multicellular Green Alga Volvox carteri Prochnik, S. E.; Umen, J.; Nedelcu, A. M. Science, Vol. 329, Issue 5988 https://doi.org/10.1126/science.1188800	journal	July 2010
Running with the Red Queen: Host-Parasite Coevolution Selects for Biparental Sex Morran, L. T.; Schmidt, O. G.; Gelarden, I. A. Science, Vol. 333, Issue 6039 https://doi.org/10.1126/science.1206360	journal	July 2011
Relaxed cellular controls and organelle heredity Birky, C. Science, Vol. 222, Issue 4623 https://doi.org/10.1126/science.6353578	journal	November 1983
MAKER2: an annotation pipeline and genome-database management tool for second-generation genome projects Holt, Carson; Yandell, Mark BMC Bioinformatics, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2105-12-491	journal	December 2011
Potential impact of stress activated retrotransposons on genome evolution in a marine diatom Maumus, Florian; Allen, Andrew E.; Mhiri, Corinne BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-624	journal	January 2009
A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae Nozaki, Hisayoshi; Takano, Hiroyoshi; Misumi, Osami BMC Biology, Vol. 5, Issue 1 https://doi.org/10.1186/1741-7007-5-28	journal	July 2007
Versatile and open software for comparing large genomes Kurtz, Stefan; Phillippy, Adam; Delcher, Arthur L. Genome Biology, Vol. 5, Issue 2, R12 https://doi.org/10.1186/gb-2004-5-2-r12	journal	January 2004
Multimodular type I polyketide synthases in algae evolve by module duplications and displacement of AT domains in trans Shelest, Ekaterina; Heimerl, Natalie; Fichtner, Maximilian BMC Genomics, Vol. 16, Issue 1 https://doi.org/10.1186/s12864-015-2222-9	journal	November 2015
An integrative analysis of post-translational histone modifications in the marine diatom Phaeodactylum tricornutum Veluchamy, Alaguraj; Rastogi, Achal; Lin, Xin Genome Biology, Vol. 16, Issue 1 https://doi.org/10.1186/s13059-015-0671-8	journal	May 2015
Recent progress and future challenges in algal biofuel production Shurin, Jonathan B.; Burkart, Michael D.; Mayfield, Stephen P. F1000Research, Vol. 5 https://doi.org/10.12688/f1000research.9217.1	journal	January 2016
Analysis of the Basidiomycete Coprinopsis cinerea Reveals Conservation of the Core Meiotic Expression Program over Half a Billion Years of Evolution Burns, Claire; Stajich, Jason E.; Rechtsteiner, Andreas PLoS Genetics, Vol. 6, Issue 9 https://doi.org/10.1371/journal.pgen.1001135	journal	September 2010
Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin: Metabolic Tools for Enhanced Algal Fitness in the Prominent Order Prymnesiales (Haptophyceae) Hovde, Blake T.; Deodato, Chloe R.; Hunsperger, Heather M. PLOS Genetics, Vol. 11, Issue 9 https://doi.org/10.1371/journal.pgen.1005469	journal	September 2015
Comparative Analyses of Three Chlorella Species in Response to Light and Sugar Reveal Distinctive Lipid Accumulation Patterns in the Microalga C. sorokiniana Rosenberg, Julian N.; Kobayashi, Naoko; Barnes, Austin PLoS ONE, Vol. 9, Issue 4 https://doi.org/10.1371/journal.pone.0092460	journal	April 2014
Solexa Ltd Bennett, Simon Pharmacogenomics, Vol. 5, Issue 4 https://doi.org/10.1517/14622416.5.4.433	journal	June 2004
Role of a Nonselective de Novo DNA Methyltransferase in Maternal Inheritance of Chloroplast Genes in the Green Alga, Chlamydomonas reinhardtii Nishiyama, Rie; Wada, Yuko; Mibu, Momoka Genetics, Vol. 168, Issue 2 https://doi.org/10.1534/genetics.104.030775	journal	October 2004
Artificial Selection: a Powerful tool for Ecologists Conner, Jeffrey K. Ecology, Vol. 84, Issue 7 https://doi.org/10.1890/0012-9658(2003)084[1650:ASAPTF]2.0.CO;2	journal	July 2003
Strategies for Optimizing Algal Biology for Enhanced Biomass Production Barry, Amanda N.; Starkenburg, Shawn R.; Sayre, Richard T. Frontiers in Energy Research, Vol. 3 https://doi.org/10.3389/fenrg.2015.00001	journal	February 2015

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Title: Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

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