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Title: Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2

Abstract

We present that most reliable transformation protocols for cereal crops, including sorghum (Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium- or particle-bombardment-mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T0 plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. In this study, we describe a system that makes use of the differential expression of the morphogenic regulators Baby Boom (Bbm) and Wuschel2 (Wus2) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods.

Authors:
 [1];  [1];  [2];  [2];  [1]
+ Show Author Affiliations
  1. Univ. of Rhode Island, Kingston, RI (United States)
  2. Univ. of Missouri, Columbia, MO (United States)
Publication Date:
Research Org.:
Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Rhode Island, Kingston, RI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1530823
Alternate Identifier(s):
OSTI ID: 1479566
Grant/Contract Number:  
SC0018277
Resource Type:
Accepted Manuscript
Journal Name:
Current Protocols in Plant Biology
Additional Journal Information:
Journal Volume: 3; Journal Issue: 4; Journal ID: ISSN 2379-8068
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Agrobacterium tumefaciens; morphogenic regulators; Sorghum bicolor; sorghum transformation

Citation Formats

Nelson-Vasilchik, Kimberly, Hague, Joel, Mookkan, Muruganantham, Zhang, Zhanyuan J., and Kausch, Albert. Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2. United States: N. p., 2018. Web. doi:10.1002/cppb.20076.
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Nelson-Vasilchik, Kimberly, Hague, Joel, Mookkan, Muruganantham, Zhang, Zhanyuan J., & Kausch, Albert. Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2. United States. https://doi.org/10.1002/cppb.20076
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Nelson-Vasilchik, Kimberly, Hague, Joel, Mookkan, Muruganantham, Zhang, Zhanyuan J., and Kausch, Albert. Wed . "Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2". United States. https://doi.org/10.1002/cppb.20076. https://www.osti.gov/servlets/purl/1530823.
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@article{osti_1530823,
title = {Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2},
author = {Nelson-Vasilchik, Kimberly and Hague, Joel and Mookkan, Muruganantham and Zhang, Zhanyuan J. and Kausch, Albert},
abstractNote = {We present that most reliable transformation protocols for cereal crops, including sorghum (Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium- or particle-bombardment-mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T0 plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. In this study, we describe a system that makes use of the differential expression of the morphogenic regulators Baby Boom (Bbm) and Wuschel2 (Wus2) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods.},
doi = {10.1002/cppb.20076},
journal = {Current Protocols in Plant Biology},
number = 4,
volume = 3,
place = {United States},
year = {Wed Oct 17 00:00:00 EDT 2018},
month = {Wed Oct 17 00:00:00 EDT 2018}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/cppb.20076
Copyright Statement
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Figures / Tables:

Figure 1 Figure 1: Diagrammatic representation of the morphogenic regulator construct PHP78891. The PHP78891 vector contains four expression cassettes: (i) Zm-RAB17pro::CRE; (ii) NOS pro::WUS2; (iii); Zm-UBIpro::BBM and (iv) Zm-UBIpro::GFP. Lox P sites bracket the CRE/WUS2/BBM cassette. After a dessication stress the RAB17 promoter activates CRE expression, resulting in the excision of themore » cassette bracketed by the loxP sites, leaving one lox site and Zm-UBIpro::GFP« less
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