Proteomic Profiling of Burkholderia thailandensis During Host Infection Using Bio-Orthogonal Noncanonical Amino Acid Tagging (BONCAT)

Franco, Magdalena; D'haeseleer, Patrik M.; Branda, Steven S.; Liou, Megan J.; Haider, Yasmeen; Segelke, Brent W.; El-Etr, Sahar H.

doi:10.3389/fcimb.2018.00370

Title: Proteomic Profiling of Burkholderia thailandensis During Host Infection Using Bio-Orthogonal Noncanonical Amino Acid Tagging (BONCAT)

Journal Article · 22 October 2018 · Frontiers in Cellular and Infection Microbiology

DOI: https://doi.org/10.3389/fcimb.2018.00370 · OSTI ID:1478535

Franco, Magdalena; D'haeseleer, Patrik M.; Branda, Steven S.; Liou, Megan J.; Haider, Yasmeen; Segelke, Brent W.; El-Etr, Sahar H.

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Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE; USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000; AC52-07NA27344

OSTI ID:: 1478535

Report Number(s):: LLNL-JRNL-751478; SAND-2018-12024J; 370

Journal Information:: Frontiers in Cellular and Infection Microbiology, Journal Name: Frontiers in Cellular and Infection Microbiology Vol. 8; ISSN 2235-2988

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

References (81)

Orthogonal Alkynyl Amino Acid Reporter for Selective Labeling of Bacterial Proteomes during Infection Grammel, Markus; Zhang, Mingzi M.; Hang, Howard C. Angewandte Chemie International Edition, Vol. 49, Issue 34 https://doi.org/10.1002/anie.201002050	journal	July 2010
What is the total number of protein molecules per cell volume? A call to rethink some published values: Insights & Perspectives Milo, Ron BioEssays, Vol. 35, Issue 12 https://doi.org/10.1002/bies.201300066	journal	September 2013
A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells Lieber, Michael; Todaro, George; Smith, Barry International Journal of Cancer, Vol. 17, Issue 1 https://doi.org/10.1002/ijc.2910170110	journal	January 1976
Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles Muangsombut, Veerachat; Suparak, Supaporn; Pumirat, Pornpan Archives of Microbiology, Vol. 190, Issue 6 https://doi.org/10.1007/s00203-008-0413-3	journal	July 2008
Burkholderia pseudomallei: animal models of infection Titball, Richard W.; Russell, Paul; Cuccui, Jon Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 102 https://doi.org/10.1016/S0035-9203(08)70026-9	journal	December 2008
Bacterial iron homeostasis Andrews, Simon C.; Robinson, Andrea K.; Rodríguez-Quiñones, Francisco FEMS Microbiology Reviews, Vol. 27, Issue 2-3 https://doi.org/10.1016/S0168-6445(03)00055-X	journal	June 2003
Common Themes in the Design and Function of Bacterial Effectors Galán, Jorge E. Cell Host & Microbe, Vol. 5, Issue 6 https://doi.org/10.1016/j.chom.2009.04.008	journal	June 2009
Antibiotic resistance in Burkholderia species Rhodes, Katherine A.; Schweizer, Herbert P. Drug Resistance Updates, Vol. 28 https://doi.org/10.1016/j.drup.2016.07.003	journal	September 2016
Type III secretion system cluster 3 is required for maximal virulence of Burkholderia pseudomallei in a hamster infection model Warawa, Jonathan; Woods, Donald E. FEMS Microbiology Letters, Vol. 242, Issue 1 https://doi.org/10.1016/j.femsle.2004.10.045	journal	January 2005
Comparative analysis of statistical methods used for detecting differential expression in label-free mass spectrometry proteomics Langley, Sarah R.; Mayr, Manuel Journal of Proteomics, Vol. 129 https://doi.org/10.1016/j.jprot.2015.07.012	journal	November 2015
Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells Kespichayawattana, Wannapa; Intachote, Pakamas; Utaisincharoen, Pongsak Microbial Pathogenesis, Vol. 36, Issue 5 https://doi.org/10.1016/j.micpath.2004.01.001	journal	May 2004
Use of ribosomal promoters from Burkholderia cenocepacia and Burkholderia cepacia for improved expression of transporter protein in Escherichia coli Yu, Manda; Tsang, Jimmy S. H. Protein Expression and Purification, Vol. 49, Issue 2 https://doi.org/10.1016/j.pep.2006.04.004	journal	October 2006
Burkholderia pseudomallei and Burkholderia mallei vaccines: Are we close to clinical trials? Titball, Richard W.; Burtnick, Mary N.; Bancroft, Gregory J. Vaccine, Vol. 35, Issue 44 https://doi.org/10.1016/j.vaccine.2017.03.022	journal	October 2017
Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei -like strains Brett, P. J.; Deshazer, D.; Woods, D. E. Epidemiology and Infection, Vol. 118, Issue 2 https://doi.org/10.1017/S095026889600739X	journal	April 1997
Global Awakening of Cryptic Biosynthetic Gene Clusters in Burkholderia thailandensis Gupta, Ashish; Bedre, Renesh; Thapa, Sudarshan Singh ACS Chemical Biology, Vol. 12, Issue 12 https://doi.org/10.1021/acschembio.7b00681	journal	November 2017
Quorum-Sensing-Regulated Bactobolin Production by Burkholderia thailandensis E264 Seyedsayamdost, Mohammad R.; Chandler, Josephine R.; Blodgett, Joshua A. V. Organic Letters, Vol. 12, Issue 4 https://doi.org/10.1021/ol902751x	journal	February 2010
Tn7: smarter than we thought Peters, Joseph E.; Craig, Nancy L. Nature Reviews Molecular Cell Biology, Vol. 2, Issue 11 https://doi.org/10.1038/35099006	journal	November 2001
Chitinase 3-like-1 enhances bacterial adhesion to colonic epithelial cells through the interaction with bacterial chitin-binding protein Kawada, Mayumi; Chen, Chun-Chuan; Arihiro, Atsuko Laboratory Investigation, Vol. 88, Issue 8 https://doi.org/10.1038/labinvest.2008.47	journal	May 2008
Robust Salmonella metabolism limits possibilities for new antimicrobials Becker, Daniel; Selbach, Matthias; Rollenhagen, Claudia Nature, Vol. 440, Issue 7082 https://doi.org/10.1038/nature04616	journal	March 2006
Revealing the hidden functional diversity of an enzyme family Bastard, Karine; Smith, Adam Alexander Thil; Vergne-Vaxelaire, Carine Nature Chemical Biology, Vol. 10, Issue 1 https://doi.org/10.1038/nchembio.1387	journal	November 2013
Cell-selective metabolic labeling of proteins Ngo, John T.; Champion, Julie A.; Mahdavi, Alborz Nature Chemical Biology, Vol. 5, Issue 10 https://doi.org/10.1038/nchembio.200	journal	August 2009
A Tn7-based broad-range bacterial cloning and expression system Choi, Kyoung-Hee; Gaynor, Jared B.; White, Kimberly G. Nature Methods, Vol. 2, Issue 6 https://doi.org/10.1038/nmeth765	journal	May 2005
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa Choi, Kyoung-Hee; Schweizer, Herbert P. Nature Protocols, Vol. 1, Issue 1 https://doi.org/10.1038/nprot.2006.24	journal	June 2006
mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 Choi, Kyoung-Hee; DeShazer, David; Schweizer, Herbert P. Nature Protocols, Vol. 1, Issue 1 https://doi.org/10.1038/nprot.2006.25	journal	June 2006
Mammalian glycosylation in immunity Marth, Jamey D.; Grewal, Prabhjit K. Nature Reviews Immunology, Vol. 8, Issue 11 https://doi.org/10.1038/nri2417	journal	November 2008
Iron and microbial infection Schaible, Ulrich E.; Kaufmann, Stefan H. E. Nature Reviews Microbiology, Vol. 2, Issue 12 https://doi.org/10.1038/nrmicro1046	journal	December 2004
Salmonellae interplay with host cells Haraga, Andrea; Ohlson, Maikke B.; Miller, Samuel I. Nature Reviews Microbiology, Vol. 6, Issue 1 https://doi.org/10.1038/nrmicro1788	journal	January 2008
Selective enrichment of mycobacterial proteins from infected host macrophages Chande, Ajit G.; Siddiqui, Zaved; Midha, Mukul Kumar Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep13430	journal	August 2015
Haem iron‐transport system in enterohaemorrhagic Escherichia coli O157:H7 Torres, Alfredo G.; Payne, Shelley M. Molecular Microbiology, Vol. 23, Issue 4 https://doi.org/10.1046/j.1365-2958.1997.2641628.x	journal	February 1997
Structure of the Shigella dysenteriae haem transport locus and its phylogenetic distribution in enteric bacteria Wyckoff, Elizabeth E.; Duncan, Donald; Torres, Alfredo G. Molecular Microbiology, Vol. 28, Issue 6 https://doi.org/10.1046/j.1365-2958.1998.00873.x	journal	June 1998
An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen: Role of a TTSS in B. pseudomallei-host cell interactions Stevens, Mark P.; Wood, Michael W.; Taylor, Lowrie A. Molecular Microbiology, Vol. 46, Issue 3 https://doi.org/10.1046/j.1365-2958.2002.03190.x	journal	October 2002
Melioidosis Wiersinga, W. Joost; Currie, Bart J.; Peacock, Sharon J. New England Journal of Medicine, Vol. 367, Issue 11 https://doi.org/10.1056/NEJMra1204699	journal	September 2012
Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo Tanrikulu, I. C.; Schmitt, E.; Mechulam, Y. Proceedings of the National Academy of Sciences, Vol. 106, Issue 36 https://doi.org/10.1073/pnas.0905735106	journal	August 2009
Identification of secreted bacterial proteins by noncanonical amino acid tagging Mahdavi, A.; Szychowski, J.; Ngo, J. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 1 https://doi.org/10.1073/pnas.1301740111	journal	December 2013
Burkholderia bacteria infectiously induce the proto-farming symbiosis of Dictyostelium amoebae and food bacteria DiSalvo, Susanne; Haselkorn, Tamara S.; Bashir, Usman Proceedings of the National Academy of Sciences, Vol. 112, Issue 36 https://doi.org/10.1073/pnas.1511878112	journal	August 2015
Discovery of scmR as a global regulator of secondary metabolism and virulence in Burkholderia thailandensis E264 Mao, Dainan; Bushin, Leah B.; Moon, Kyuho Proceedings of the National Academy of Sciences, Vol. 114, Issue 14 https://doi.org/10.1073/pnas.1619529114	journal	March 2017
Structural Insights into an Oxalate-producing Serine Hydrolase with an Unusual Oxyanion Hole and Additional Lyase Activity Oh, Juntaek; Hwang, Ingyu; Rhee, Sangkee Journal of Biological Chemistry, Vol. 291, Issue 29 https://doi.org/10.1074/jbc.M116.727180	journal	May 2016
Proteomic Analysis of Salmonella enterica Serovar Typhimurium Isolated from RAW 264.7 Macrophages Shi, Liang; Adkins, Joshua N.; Coleman, James R. Journal of Biological Chemistry, Vol. 281, Issue 39 https://doi.org/10.1074/jbc.M604640200	journal	August 2006
A hemolytic pigment of Group B Streptococcus allows bacterial penetration of human placenta Whidbey, Christopher; Harrell, Maria Isabel; Burnside, Kellie The Journal of Experimental Medicine, Vol. 210, Issue 6 https://doi.org/10.1084/jem.20122753	journal	May 2013
The Pseudomallei Group: A Review Howe, C.; Sampath, A.; Spotnitz, M. Journal of Infectious Diseases, Vol. 124, Issue 6 https://doi.org/10.1093/infdis/124.6.598	journal	December 1971
The Protein Data Bank Berman, H. M. Nucleic Acids Research, Vol. 28, Issue 1 https://doi.org/10.1093/nar/28.1.235	journal	January 2000
Operon prediction using both genome-specific and general genomic information Dam, Phuongan; Olman, Victor; Harris, Kyle Nucleic Acids Research, Vol. 35, Issue 1 https://doi.org/10.1093/nar/gkl1018	journal	December 2006
DOOR: a database for prokaryotic operons Mao, Fenglou; Dam, Phuongan; Chou, Jacky Nucleic Acids Research, Vol. 37, Issue suppl_1 https://doi.org/10.1093/nar/gkn757	journal	November 2008
2016 update of the PRIDE database and its related tools Vizcaíno, Juan Antonio; Csordas, Attila; del-Toro, Noemi Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1145	journal	November 2015
VFDB 2016: hierarchical and refined dataset for big data analysis—10 years on Chen, Lihong; Zheng, Dandan; Liu, Bo Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1239	journal	November 2015
The STRING database in 2017: quality-controlled protein–protein association networks, made broadly accessible Szklarczyk, Damian; Morris, John H.; Cook, Helen Nucleic Acids Research, Vol. 45, Issue D1 https://doi.org/10.1093/nar/gkw937	journal	October 2016
Note: Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species Brett, P. J.; DeShazer, D.; Woods, D. E. International Journal of Systematic Bacteriology, Vol. 48, Issue 1 https://doi.org/10.1099/00207713-48-1-317	journal	January 1998
Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei Rainbow, Lucille; Hart, C. Anthony; Winstanley, Craig Journal of Medical Microbiology, Vol. 51, Issue 5 https://doi.org/10.1099/0022-1317-51-5-374	journal	May 2002
Bacterial chitinases and chitin-binding proteins as virulence factors Frederiksen, R. F.; Paspaliari, D. K.; Larsen, T. Microbiology, Vol. 159, Issue Pt_5 https://doi.org/10.1099/mic.0.051839-0	journal	March 2013
Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis Stevens, M. P. Microbiology, Vol. 150, Issue 8 https://doi.org/10.1099/mic.0.27146-0	journal	August 2004
Tn7 recognizes transposition target structures associated with DNA replication using the DNA-binding protein TnsE Peters, J. E. Genes & Development, Vol. 15, Issue 6 https://doi.org/10.1101/gad.870201	journal	March 2001
Tn7: a target site-specific transposon Craig, N. L. Molecular Microbiology, Vol. 5, Issue 11 https://doi.org/10.1111/j.1365-2958.1991.tb01964.x	journal	November 1991
Virulence of clinical and environmental isolates of Burkholderia oklahomensis and Burkholderia thailandensis in hamsters and mice DeShazer, David FEMS Microbiology Letters, Vol. 277, Issue 1 https://doi.org/10.1111/j.1574-6968.2007.00946.x	journal	December 2007
In Vitro Susceptibilities of Burkholderia mallei in Comparison to Those of Other Pathogenic Burkholderia spp. Kenny, D. J.; Russell, P.; Rogers, D. Antimicrobial Agents and Chemotherapy, Vol. 43, Issue 11 https://doi.org/10.1128/AAC.43.11.2773	journal	November 1999
Glyphosate Resistance as a Novel Select-Agent-Compliant, Non-Antibiotic-Selectable Marker in Chromosomal Mutagenesis of the Essential Genes asd and dapB of Burkholderia pseudomallei Norris, M. H.; Kang, Y.; Lu, D. Applied and Environmental Microbiology, Vol. 75, Issue 19 https://doi.org/10.1128/AEM.00820-09	journal	July 2009
Genetic Tools for Select-Agent-Compliant Manipulation of Burkholderia pseudomallei Choi, K. -H.; Mima, T.; Casart, Y. Applied and Environmental Microbiology, Vol. 74, Issue 4 https://doi.org/10.1128/AEM.02430-07	journal	December 2007
Burkholderia thailandensis as a Model System for the Study of the Virulence-Associated Type III Secretion System of Burkholderia pseudomallei Haraga, A.; West, T. E.; Brittnacher, M. J. Infection and Immunity, Vol. 76, Issue 11 https://doi.org/10.1128/IAI.00626-08	journal	September 2008
Role of Host Xanthine Oxidase in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coli Crane, John K.; Naeher, Tonniele M.; Broome, Jacqueline E. Infection and Immunity, Vol. 81, Issue 4 https://doi.org/10.1128/IAI.01124-12	journal	January 2013
The Putative Glycosyltransferase-Encoding Gene cylJ and the Group B Streptococcus (GBS)-Specific Gene cylK Modulate Hemolysin Production and Virulence of GBS Forquin, M. -P.; Tazi, A.; Rosa-Fraile, M. Infection and Immunity, Vol. 75, Issue 4 https://doi.org/10.1128/IAI.01565-06	journal	February 2007
The Animal Pathogen-Like Type III Secretion System Is Required for the Intracellular Survival of Burkholderia mallei within J774.2 Macrophages Ribot, W. J.; Ulrich, R. L. Infection and Immunity, Vol. 74, Issue 7 https://doi.org/10.1128/IAI.01939-05	journal	June 2006
Arabinose assimilation defines a nonvirulent biotype of Burkholderia pseudomallei. Smith, M. D.; Angus, B. J.; Wuthiekanun, V. Infection and immunity, Vol. 65, Issue 10 https://doi.org/10.1128/IAI.65.10.4319-4321.1997	journal	January 1997
PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa. Cota-Gomez, A.; Vasil, A. I.; Kadurugamuwa, J. Infection and immunity, Vol. 65, Issue 7 https://doi.org/10.1128/IAI.65.7.2904-2913.1997	journal	January 1997
Xanthine Oxidase Contributes to Host Defense against Burkholderia cepacia in the p47phox-/- Mouse Model of Chronic Granulomatous Disease Segal, B. H.; Sakamoto, N.; Patel, M. Infection and Immunity, Vol. 68, Issue 4 https://doi.org/10.1128/IAI.68.4.2374-2378.2000	journal	April 2000
Type III Secretion: a Virulence Factor Delivery System Essential for the Pathogenicity of Burkholderia mallei Ulrich, R. L.; DeShazer, D. Infection and Immunity, Vol. 72, Issue 2 https://doi.org/10.1128/IAI.72.2.1150-1154.2004	journal	January 2004
Role of Xanthine Oxidoreductase as an Antimicrobial Agent Martin, H. M.; Hancock, J. T.; Salisbury, V. Infection and Immunity, Vol. 72, Issue 9 https://doi.org/10.1128/IAI.72.9.4933-4939.2004	journal	August 2004
Global Analysis of the Burkholderia thailandensis Quorum Sensing-Controlled Regulon Majerczyk, C.; Brittnacher, M.; Jacobs, M. Journal of Bacteriology, Vol. 196, Issue 7 https://doi.org/10.1128/JB.01405-13	journal	January 2014
Anr and Its Activation by PlcH Activity in Pseudomonas aeruginosa Host Colonization and Virulence Jackson, A. A.; Gross, M. J.; Daniels, E. F. Journal of Bacteriology, Vol. 195, Issue 13 https://doi.org/10.1128/JB.02169-12	journal	May 2013
Actin-Binding Proteins from Burkholderia mallei and Burkholderia thailandensis Can Functionally Compensate for the Actin-Based Motility Defect of a Burkholderia pseudomallei bimA Mutant Stevens, J. M.; Ulrich, R. L.; Taylor, L. A. Journal of Bacteriology, Vol. 187, Issue 22 https://doi.org/10.1128/JB.187.22.7857-7862.2005	journal	November 2005
Genetics and regulation of heme iron transport in Shigella dysenteriae and detection of an analogous system in Escherichia coli O157:H7. Mills, M.; Payne, S. M. Journal of Bacteriology, Vol. 177, Issue 11 https://doi.org/10.1128/jb.177.11.3004-3009.1995	journal	June 1995
Within-Host Evolution of Burkholderia pseudomallei over a Twelve-Year Chronic Carriage Infection Price, Erin P.; Sarovich, Derek S.; Mayo, Mark mBio, Vol. 4, Issue 4 https://doi.org/10.1128/mBio.00388-13	journal	July 2013
The Listeria monocytogenes ChiA Chitinase Enhances Virulence through Suppression of Host Innate Immunity Chaudhuri, Swarnava; Gantner, Benjamin N.; Ye, Richard D. mBio, Vol. 4, Issue 2 https://doi.org/10.1128/mBio.00617-12	journal	March 2013
New Method for the Orthogonal Labeling and Purification of Toxoplasma gondii Proteins While Inside the Host Cell Wier, Gregory M.; McGreevy, Erica M.; Brown, Mark J. mBio, Vol. 6, Issue 2 https://doi.org/10.1128/mBio.01628-14	journal	March 2015
Molecular Insights into Burkholderia pseudomallei and Burkholderia mallei Pathogenesis Galyov, Edouard E.; Brett, Paul J.; DeShazer, David Annual Review of Microbiology, Vol. 64, Issue 1 https://doi.org/10.1146/annurev.micro.112408.134030	journal	October 2010
Strategies of Intracellular Pathogens for Obtaining Iron from the Environment Leon-Sicairos, Nidia; Reyes-Cortes, Ruth; Guadrón-Llanos, Alma M. BioMed Research International, Vol. 2015 https://doi.org/10.1155/2015/476534	journal	January 2015
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Love, Michael I.; Huber, Wolfgang; Anders, Simon Genome Biology, Vol. 15, Issue 12 https://doi.org/10.1186/s13059-014-0550-8	journal	December 2014
Genetic Incorporation of Unnatural Amino Acids into Proteins in Mycobacterium tuberculosis Wang, Feng; Robbins, Scott; Guo, Jiantao PLoS ONE, Vol. 5, Issue 2 https://doi.org/10.1371/journal.pone.0009354	journal	February 2010
The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces Wong, Edmond; Vaaje-Kolstad, Gustav; Ghosh, Avishek PLoS Pathogens, Vol. 8, Issue 1 https://doi.org/10.1371/journal.ppat.1002373	journal	January 2012
Role of spectral counting in quantitative proteomics Lundgren, Deborah H.; Hwang, Sun-Il; Wu, Linfeng Expert Review of Proteomics, Vol. 7, Issue 1 https://doi.org/10.1586/epr.09.69	journal	February 2010
Mechanisms of antibiotic resistance in Burkholderia pseudomallei : implications for treatment of melioidosis Schweizer, Herbert P. Future Microbiology, Vol. 7, Issue 12 https://doi.org/10.2217/fmb.12.116	journal	December 2012
Proteogenomics in Aid of Host–Pathogen Interaction Studies: A Bacterial Perspective Fels, Ursula; Gevaert, Kris; Van Damme, Petra Proteomes, Vol. 5, Issue 4 https://doi.org/10.3390/proteomes5040026	journal	October 2017
Pathogenesis of Burkholderia pseudomallei and Burkholderia mallei Larsen, Joseph C.; Johnson, Nathan H. Military Medicine, Vol. 174, Issue 6 https://doi.org/10.7205/MILMED-D-03-0808	journal	June 2009

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