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Title: Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging

Abstract

The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. Here, the results show the potential of ELDI-MSI to provide spatial informationmore » for a variety of plant metabolites with little sample preparation.« less

Authors:
 [1];  [1];  [1];  [2];  [3];  [1];  [1]
  1. Ames Lab. and Iowa State Univ., Ames, IA (United States)
  2. Ames Lab., Ames, IA (United States)
  3. Ames Lab. and Iowa State Univ., Ames, IA (United States); Kemin Industries Inc., Des Moines, IA (United States)
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1478236
Report Number(s):
IS-J-9768
Journal ID: ISSN 1664-462X
Grant/Contract Number:  
AC02-07CH11358
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; matrix-free imaging; mass spectrometry imaging; electrospray laser desorption ionization; shading; Coleus (Solenostemon scuttellarioides); anthocyanin; flavonoid; source-to-sink

Citation Formats

McVey, Patrick A., Alexander, Liza E., Fu, Xinyu, Xie, Bo, Galayda, Katherine -Jo, Nikolau, Basil J., and Houk, Robert S. Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging. United States: N. p., 2018. Web. doi:10.3389/fpls.2018.01348.
McVey, Patrick A., Alexander, Liza E., Fu, Xinyu, Xie, Bo, Galayda, Katherine -Jo, Nikolau, Basil J., & Houk, Robert S. Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging. United States. doi:10.3389/fpls.2018.01348.
McVey, Patrick A., Alexander, Liza E., Fu, Xinyu, Xie, Bo, Galayda, Katherine -Jo, Nikolau, Basil J., and Houk, Robert S. Wed . "Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging". United States. doi:10.3389/fpls.2018.01348. https://www.osti.gov/servlets/purl/1478236.
@article{osti_1478236,
title = {Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging},
author = {McVey, Patrick A. and Alexander, Liza E. and Fu, Xinyu and Xie, Bo and Galayda, Katherine -Jo and Nikolau, Basil J. and Houk, Robert S.},
abstractNote = {The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. Here, the results show the potential of ELDI-MSI to provide spatial information for a variety of plant metabolites with little sample preparation.},
doi = {10.3389/fpls.2018.01348},
journal = {Frontiers in Plant Science},
number = ,
volume = 9,
place = {United States},
year = {2018},
month = {9}
}

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