The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy
Abstract
In situ characterization of the chemical and structural properties of black and white sheep hair was performed with a spatial resolution of 25 nm using infrared nano-spectroscopy. Comparing data sets from two types of hair allowed us to isolate the keratin FTIR fingerprint and so mark off chemical properties of the hair's melanosomes. From a polarization sensitive analysis of the nano-FTIR spectra, we showed that keratin intermediate filaments (IFs) present anisotropic molecular ordering. In stark contrast with white hair which does not contain melanosomes, in black hair, we spatially resolved single melanosomes and achieved unprecedented assignment of the vibrational modes of pheomelanin and eumelanin. The in situ experiment presented here avoids harsh chemical extractive methods used in previous studies. Lastly, our findings offer a basis for a better understanding of the keratin chemical and structural packing in different hair phenotypes as well as the involvement of melanosomes in hair color and biological functionality.
- Authors:
-
- Brazilian Synchrotron Light Lab., Campinas (Brazil)
- Brazilian National Nanotechnology Lab., Campinas (Brazil)
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Publication Date:
- Research Org.:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1477957
- Report Number(s):
- BNL-209180-2018-JAAM
Journal ID: ISSN 2040-3364; NANOHL
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nanoscale
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 29; Journal ID: ISSN 2040-3364
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Stanic, Vesna, Maia, Francisco Carlos Barbosa, Freitas, Raul de Oliveira, Montoro, Fabiano Emmanuel, and Evans-Lutterodt, Kenneth. The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy. United States: N. p., 2018.
Web. doi:10.1039/C8NR03146K.
Stanic, Vesna, Maia, Francisco Carlos Barbosa, Freitas, Raul de Oliveira, Montoro, Fabiano Emmanuel, & Evans-Lutterodt, Kenneth. The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy. United States. https://doi.org/10.1039/C8NR03146K
Stanic, Vesna, Maia, Francisco Carlos Barbosa, Freitas, Raul de Oliveira, Montoro, Fabiano Emmanuel, and Evans-Lutterodt, Kenneth. Mon .
"The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy". United States. https://doi.org/10.1039/C8NR03146K. https://www.osti.gov/servlets/purl/1477957.
@article{osti_1477957,
title = {The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy},
author = {Stanic, Vesna and Maia, Francisco Carlos Barbosa and Freitas, Raul de Oliveira and Montoro, Fabiano Emmanuel and Evans-Lutterodt, Kenneth},
abstractNote = {In situ characterization of the chemical and structural properties of black and white sheep hair was performed with a spatial resolution of 25 nm using infrared nano-spectroscopy. Comparing data sets from two types of hair allowed us to isolate the keratin FTIR fingerprint and so mark off chemical properties of the hair's melanosomes. From a polarization sensitive analysis of the nano-FTIR spectra, we showed that keratin intermediate filaments (IFs) present anisotropic molecular ordering. In stark contrast with white hair which does not contain melanosomes, in black hair, we spatially resolved single melanosomes and achieved unprecedented assignment of the vibrational modes of pheomelanin and eumelanin. The in situ experiment presented here avoids harsh chemical extractive methods used in previous studies. Lastly, our findings offer a basis for a better understanding of the keratin chemical and structural packing in different hair phenotypes as well as the involvement of melanosomes in hair color and biological functionality.},
doi = {10.1039/C8NR03146K},
journal = {Nanoscale},
number = 29,
volume = 10,
place = {United States},
year = {Mon Jul 16 00:00:00 EDT 2018},
month = {Mon Jul 16 00:00:00 EDT 2018}
}
Web of Science
Works referenced in this record:
Infrared spectroscopy of proteins
journal, September 2007
- Barth, Andreas
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1767, Issue 9
Near-field probing of vibrational absorption for chemical microscopy
journal, May 1999
- Knoll, B.; Keilmann, F.
- Nature, Vol. 399, Issue 6732
Degradation of the ethyl glucuronide content in hair by hydrogen peroxide and a non-destructive assay for oxidative hair treatment using infra-red spectroscopy
journal, November 2014
- Ammann, Dominic; Becker, Roland; Kohl, Anka
- Forensic Science International, Vol. 244
Contributions to the chemistry of human hair. 1. Analyses of cystine, cysteine and cystine oxides in untreated human hair
journal, June 1987
- Hilterhaus-Bong, Sabine; Zahn, Helmut
- International Journal of Cosmetic Science, Vol. 9, Issue 3
Colour-producing β-keratin nanofibres in blue penguin ( Eudyptula minor ) feathers
journal, February 2011
- D'Alba, Liliana; Saranathan, Vinodkumar; Clarke, Julia A.
- Biology Letters, Vol. 7, Issue 4
3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography
journal, August 2013
- Martin, Michael C.; Dabat-Blondeau, Charlotte; Unger, Miriam
- Nature Methods, Vol. 10, Issue 9
Broadband near-field infrared spectromicroscopy using photothermal probes and synchrotron radiation
journal, January 2016
- Donaldson, Paul M.; Kelley, Chris S.; Frogley, Mark D.
- Optics Express, Vol. 24, Issue 3
Bacteria or melanosomes? A geochemical analysis of micro-bodies on a tadpole from the Oligocene Enspel Formation of Germany
journal, November 2014
- Barden, Holly E.; Bergmann, Uwe; Edwards, Nicholas P.
- Palaeobiodiversity and Palaeoenvironments, Vol. 95, Issue 1
Fossil Evidence for Evolution of the Shape and Color of Penguin Feathers
journal, September 2010
- Clarke, J. A.; Ksepka, D. T.; Salas-Gismondi, R.
- Science, Vol. 330, Issue 6006
Tip-enhanced infrared nanospectroscopy via molecular expansion force detection
journal, January 2014
- Lu, Feng; Jin, Mingzhou; Belkin, Mikhail A.
- Nature Photonics, Vol. 8, Issue 4
Scanning Interferometric Apertureless Microscopy: Optical Imaging at 10 Angstrom Resolution
journal, August 1995
- Zenhausern, F.; Martin, Y.; Wickramasinghe, H. K.
- Science, Vol. 269, Issue 5227
Abnormal pigmentation within cutaneous scars: A complication of wound healing
journal, May 2012
- Chadwick, Sarah; Heath, Rebecca; Shah, Mamta
- Indian Journal of Plastic Surgery, Vol. 45, Issue 02
Infrared-spectroscopic nanoimaging with a thermal source
journal, April 2011
- Huth, F.; Schnell, M.; Wittborn, J.
- Nature Materials, Vol. 10, Issue 5
Polarization Properties in Apertureless-Type Scanning Near-Field Optical Microscopy
journal, September 2015
- Ishibashi, Takayuki; Cai, Yongfu
- Nanoscale Research Letters, Vol. 10, Issue 1
Low-aberration beamline optics for synchrotron infrared nanospectroscopy
journal, January 2018
- Freitas, Raul O.; Deneke, Christoph; Maia, Francisco C. B.
- Optics Express, Vol. 26, Issue 9
Nanoscale simultaneous chemical and mechanical imaging via peak force infrared microscopy
journal, June 2017
- Wang, Le; Wang, Haomin; Wagner, Martin
- Science Advances, Vol. 3, Issue 6
Evidence for various calcium sites in human hair shaft revealed by sub-micrometer X-ray fluorescence
journal, January 2003
- Mérigoux, C.; Briki, F.; Sarrot-Reynauld, F.
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1619, Issue 1
Infrared Fingerprints of Natural 2D Talc and Plasmon–Phonon Coupling in Graphene–Talc Heterostructures
journal, April 2018
- Barcelos, Ingrid D.; Cadore, Alisson R.; Alencar, Ananias B.
- ACS Photonics, Vol. 5, Issue 5
Near-field imaging and nano-Fourier-transform infrared spectroscopy using broadband synchrotron radiation
journal, January 2013
- Hermann, Peter; Hoehl, Arne; Patoka, Piotr
- Optics Express, Vol. 21, Issue 3
Local structure of human hair spatially resolved by sub-micron X-ray beam
journal, November 2015
- Stanić, Vesna; Bettini, Jefferson; Montoro, Fabiano Emmanuel
- Scientific Reports, Vol. 5, Issue 1
Three-dimensional architecture of macrofibrils in the human scalp hair cortex
journal, March 2014
- Harland, Duane P.; Walls, Richard J.; Vernon, James A.
- Journal of Structural Biology, Vol. 185, Issue 3
Synchrotron X-ray muprobe and its application to human hair analysis
journal, July 1993
- Iida, Atsuo; Noma, Takashi
- Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 82, Issue 1
Nano-FTIR chemical mapping of minerals in biological materials
journal, January 2012
- Amarie, Sergiu; Zaslansky, Paul; Kajihara, Yusuke
- Beilstein Journal of Nanotechnology, Vol. 3
Microspectroscopic soft X-ray analysis of keratin based biofibers
journal, March 2015
- Späth, Andreas; Meyer, Markus; Semmler, Sonja
- Micron, Vol. 70
Hyperspectral infrared nanoimaging of organic samples based on Fourier transform infrared nanospectroscopy
journal, February 2017
- Amenabar, Iban; Poly, Simon; Goikoetxea, Monika
- Nature Communications, Vol. 8, Issue 1
Protein Secondary Structure Imaging with Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy
journal, January 2012
- Bito, Kotatsu; Okuno, Masanari; Kano, Hideaki
- The Journal of Physical Chemistry B, Vol. 116, Issue 4
Secondary structure estimation and properties analysis of stretched Asian and Caucasian hair
journal, July 2014
- Zhou, A. J.; Liu, H. L.; Du, Z. Q.
- Skin Research and Technology, Vol. 21, Issue 1
Graphene/h-BN plasmon–phonon coupling and plasmon delocalization observed by infrared nano-spectroscopy
journal, January 2015
- Barcelos, Ingrid D.; Cadore, Alisson R.; Campos, Leonardo C.
- Nanoscale, Vol. 7, Issue 27
Chemical imaging of biological tissue with synchrotron infrared light
journal, July 2006
- Miller, Lisa M.; Dumas, Paul
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 7
Investigating the origins of nanostructural variations in differential ethnic hair types using X-ray scattering techniques
journal, June 2013
- Wade, M.; Tucker, I.; Cunningham, P.
- International Journal of Cosmetic Science, Vol. 35, Issue 5
The Structure of Hair, Muscle, and Related Proteins
journal, May 1951
- Pauling, L.; Corey, R. B.
- Proceedings of the National Academy of Sciences, Vol. 37, Issue 5
Nano-Scale Morphology of Melanosomes Revealed by Small-Angle X-Ray Scattering
journal, March 2014
- Gorniak, Thomas; Haraszti, Tamas; Garamus, Vasyl M.
- PLoS ONE, Vol. 9, Issue 3
Local infrared microspectroscopy with subwavelength spatial resolution with an atomic force microscope tip used as a photothermal sensor
journal, January 2005
- Dazzi, A.; Prazeres, R.; Glotin, F.
- Optics Letters, Vol. 30, Issue 18
Theory of infrared nanospectroscopy by photothermal induced resonance
journal, June 2010
- Dazzi, A.; Glotin, F.; Carminati, R.
- Journal of Applied Physics, Vol. 107, Issue 12
Oxygen impact on the electronic and vibrational properties of black phosphorus probed by synchrotron infrared nanospectroscopy
journal, August 2017
- Grasseschi, D.; Bahamon, D. A.; Maia, F. C. B.
- 2D Materials, Vol. 4, Issue 3
Fifty years of fibrous protein research: A personal retrospective
journal, June 2014
- Parry, David A. D.
- Journal of Structural Biology, Vol. 186, Issue 3
Infrared and Water Sorption Studies of the Hydration Structure and Mechanism in Natural and Synthetic Melanin
journal, July 2010
- Bridelli, Maria Grazia; Crippa, Pier Raimondo
- The Journal of Physical Chemistry B, Vol. 114, Issue 29
Scanning probe optical microscopy using a metallic probe tip
journal, February 1995
- Kawata, S.; Inouye, Y.
- Ultramicroscopy, Vol. 57, Issue 2-3
Vibrational nano-spectroscopic imaging correlating structure with intermolecular coupling and dynamics
journal, April 2014
- Pollard, Benjamin; Muller, Eric A.; Hinrichs, Karsten
- Nature Communications, Vol. 5, Issue 1
Isolation of melanosomes from keratinous structures: current state of the art
journal, November 1986
- Borovansk�, J.; Hach, P.
- Archives of Dermatological Research, Vol. 279, Issue 1
Structural analysis and mapping of individual protein complexes by infrared nanospectroscopy
journal, December 2013
- Amenabar, Iban; Poly, Simon; Nuansing, Wiwat
- Nature Communications, Vol. 4, Issue 1
Infrared vibrational nanocrystallography and nanoimaging
journal, October 2016
- Muller, Eric A.; Pollard, Benjamin; Bechtel, Hans A.
- Science Advances, Vol. 2, Issue 10
Nanoscale plasmonic phenomena in CVD-grown MoS_2 monolayer revealed by ultra-broadband synchrotron radiation based nano-FTIR spectroscopy and near-field microscopy
journal, January 2016
- Patoka, Piotr; Ulrich, Georg; Nguyen, Ariana E.
- Optics Express, Vol. 24, Issue 2
Characterization of semiconductor materials using synchrotron radiation-based near-field infrared microscopy and nano-FTIR spectroscopy
journal, January 2014
- Hermann, Peter; Hoehl, Arne; Ulrich, Georg
- Optics Express, Vol. 22, Issue 15
Chemical, experimental, and morphological evidence for diagenetically altered melanin in exceptionally preserved fossils
journal, September 2015
- Colleary, Caitlin; Dolocan, Andrei; Gardner, James
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 41
Precise colocalization of interacting structural and pigmentary elements generates extensive color pattern variation in Phelsuma lizards
journal, January 2013
- Saenko, Suzanne V.; Teyssier, Jérémie; van der Marel, Dirk
- BMC Biology, Vol. 11, Issue 1
Lipids in keratinized tissues
journal, July 1963
- Fraser, R. D. B.; MacRae, T. P.; Rogers, G. E.
- Journal of Molecular Biology, Vol. 7, Issue 1
Nano-FTIR Absorption Spectroscopy of Molecular Fingerprints at 20 nm Spatial Resolution
journal, July 2012
- Huth, Florian; Govyadinov, Alexander; Amarie, Sergiu
- Nano Letters, Vol. 12, Issue 8
Ultrabroadband infrared nanospectroscopic imaging
journal, May 2014
- Bechtel, H. A.; Muller, E. A.; Olmon, R. L.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 20
Midinfrared absorption measured at a λ/400 resolution with an atomic force microscope
journal, January 2009
- Houel, Julien; Homeyer, Estelle; Sauvage, Sébastien
- Optics Express, Vol. 17, Issue 13
Structure, function, and self-assembly of single network gyroid (I4132) photonic crystals in butterfly wing scales
journal, June 2010
- Saranathan, V.; Osuji, C. O.; Mochrie, S. G. J.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 26
Opto-nanomechanical spectroscopic material characterization
journal, August 2015
- Tetard, L.; Passian, A.; Farahi, R. H.
- Nature Nanotechnology, Vol. 10, Issue 10
Elemental characterisation of melanin in feathers via synchrotron X-ray imaging and absorption spectroscopy
journal, September 2016
- Edwards, Nicholas P.; van Veelen, Arjen; Anné, Jennifer
- Scientific Reports, Vol. 6, Issue 1
Infrared Vibrational Nanospectroscopy by Self-Referenced Interferometry
journal, December 2015
- Pollard, Benjamin; Maia, Francisco C. B.; Raschke, Markus B.
- Nano Letters, Vol. 16, Issue 1
Near-field microscopy by elastic light scattering from a tip
journal, April 2004
- Keilmann, Fritz; Hillenbrand, Rainer
- Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences, Vol. 362, Issue 1817
Amplitude- and Phase-Resolved Nanospectral Imaging of Phonon Polaritons in Hexagonal Boron Nitride
journal, June 2015
- Shi, Zhiwen; Bechtel, Hans A.; Berweger, Samuel
- ACS Photonics, Vol. 2, Issue 7
Photonic crystals cause active colour change in chameleons
journal, March 2015
- Teyssier, Jérémie; Saenko, Suzanne V.; van der Marel, Dirk
- Nature Communications, Vol. 6, Issue 1
Individual colour patches as multicomponent signals
journal, January 1999
- Grether, Gregory F.; Kolluru, Gita R.; Nersissian, Karen
- Biological Reviews, Vol. 79, Issue 3
Nano-FTIR chemical mapping of minerals in biological materials
journal, July 2012
- Amarie, S.; Keilmann, F.; Zaslansky, P.
- Microscopy and Microanalysis, Vol. 18, Issue S2
Nano-scale morphology of melanosomes revealed by small-angle X-ray scattering
text, January 2014
- Gorniak, T.; Haraszti, T.; Garamus, V. M.
- Karlsruhe
Works referencing / citing this record:
Modern Scattering‐Type Scanning Near‐Field Optical Microscopy for Advanced Material Research
journal, April 2019
- Chen, Xinzhong; Hu, Debo; Mescall, Ryan
- Advanced Materials