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Title: The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy

Abstract

In situ characterization of the chemical and structural properties of black and white sheep hair was performed with a spatial resolution of 25 nm using infrared nano-spectroscopy. Comparing data sets from two types of hair allowed us to isolate the keratin FTIR fingerprint and so mark off chemical properties of the hair's melanosomes. From a polarization sensitive analysis of the nano-FTIR spectra, we showed that keratin intermediate filaments (IFs) present anisotropic molecular ordering. In stark contrast with white hair which does not contain melanosomes, in black hair, we spatially resolved single melanosomes and achieved unprecedented assignment of the vibrational modes of pheomelanin and eumelanin. The in situ experiment presented here avoids harsh chemical extractive methods used in previous studies. Lastly, our findings offer a basis for a better understanding of the keratin chemical and structural packing in different hair phenotypes as well as the involvement of melanosomes in hair color and biological functionality.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3]
  1. Brazilian Synchrotron Light Lab., Campinas (Brazil)
  2. Brazilian National Nanotechnology Lab., Campinas (Brazil)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1477957
Report Number(s):
BNL-209180-2018-JAAM
Journal ID: ISSN 2040-3364; NANOHL
Grant/Contract Number:  
SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
Nanoscale
Additional Journal Information:
Journal Volume: 10; Journal Issue: 29; Journal ID: ISSN 2040-3364
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Stanic, Vesna, Maia, Francisco Carlos Barbosa, Freitas, Raul de Oliveira, Montoro, Fabiano Emmanuel, and Evans-Lutterodt, Kenneth. The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy. United States: N. p., 2018. Web. doi:10.1039/C8NR03146K.
Stanic, Vesna, Maia, Francisco Carlos Barbosa, Freitas, Raul de Oliveira, Montoro, Fabiano Emmanuel, & Evans-Lutterodt, Kenneth. The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy. United States. doi:https://doi.org/10.1039/C8NR03146K
Stanic, Vesna, Maia, Francisco Carlos Barbosa, Freitas, Raul de Oliveira, Montoro, Fabiano Emmanuel, and Evans-Lutterodt, Kenneth. Mon . "The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy". United States. doi:https://doi.org/10.1039/C8NR03146K. https://www.osti.gov/servlets/purl/1477957.
@article{osti_1477957,
title = {The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy},
author = {Stanic, Vesna and Maia, Francisco Carlos Barbosa and Freitas, Raul de Oliveira and Montoro, Fabiano Emmanuel and Evans-Lutterodt, Kenneth},
abstractNote = {In situ characterization of the chemical and structural properties of black and white sheep hair was performed with a spatial resolution of 25 nm using infrared nano-spectroscopy. Comparing data sets from two types of hair allowed us to isolate the keratin FTIR fingerprint and so mark off chemical properties of the hair's melanosomes. From a polarization sensitive analysis of the nano-FTIR spectra, we showed that keratin intermediate filaments (IFs) present anisotropic molecular ordering. In stark contrast with white hair which does not contain melanosomes, in black hair, we spatially resolved single melanosomes and achieved unprecedented assignment of the vibrational modes of pheomelanin and eumelanin. The in situ experiment presented here avoids harsh chemical extractive methods used in previous studies. Lastly, our findings offer a basis for a better understanding of the keratin chemical and structural packing in different hair phenotypes as well as the involvement of melanosomes in hair color and biological functionality.},
doi = {10.1039/C8NR03146K},
journal = {Nanoscale},
number = 29,
volume = 10,
place = {United States},
year = {2018},
month = {7}
}

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