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Title: Botulinum Neurotoxin-Producing Bacteria. Isn’t It Time that We Called a Species a Species?

Abstract

Botulinum neurotoxins (BoNTs) are produced by a diverse set of seven clostridial species, though alternate naming systems have developed over the last 100 years. Starting in the 1950s, a single-species taxonomy where any bacterium producing BoNT would be designated Clostridium botulinum was introduced. As the extreme diversity of these strains was recognized, a secondary system of taxonomic “groups” evolved. It became clear that these groups also had members that did not produce BoNT, and in some cases, they were given formal species names. Genomic analysis now clearly identifies species affiliations whether an isolate is toxigenic or not. It is clear that C. botulinum group nomenclature is no longer appropriate and that there are recognized species names for each clostridium. We advocate for the use of the scientific binomials and that the single-species group nomenclature be abandoned.

Authors:
 [1];  [1];  [2];  [1]; ORCiD logo [1]
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  1. Northern Arizona Univ., Flagstaff, AZ (United States). The Pathogen and Microbiome Inst.
  2. Northern Arizona Univ., Flagstaff, AZ (United States). The Pathogen and Microbiome Inst.; Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1477671
Report Number(s):
LA-UR-18-27452
Journal ID: ISSN 2150-7511
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Accepted Manuscript
Journal Name:
mBio (Online)
Additional Journal Information:
Journal Name: mBio (Online); Journal Volume: 9; Journal Issue: 5; Journal ID: ISSN 2150-7511
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Clostridium botulinum; botulism; botulinum neurotoxin; phylogenetic analysis; taxonomy

Citation Formats

Smith, Theresa, Williamson, Charles H. D., Hill, Karen, Sahl, Jason, and Keim, Paul. Botulinum Neurotoxin-Producing Bacteria. Isn’t It Time that We Called a Species a Species?. United States: N. p., 2018. Web. doi:10.1128/mBio.01469-18.
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Smith, Theresa, Williamson, Charles H. D., Hill, Karen, Sahl, Jason, & Keim, Paul. Botulinum Neurotoxin-Producing Bacteria. Isn’t It Time that We Called a Species a Species?. United States. https://doi.org/10.1128/mBio.01469-18
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Smith, Theresa, Williamson, Charles H. D., Hill, Karen, Sahl, Jason, and Keim, Paul. Tue . "Botulinum Neurotoxin-Producing Bacteria. Isn’t It Time that We Called a Species a Species?". United States. https://doi.org/10.1128/mBio.01469-18. https://www.osti.gov/servlets/purl/1477671.
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@article{osti_1477671,
title = {Botulinum Neurotoxin-Producing Bacteria. Isn’t It Time that We Called a Species a Species?},
author = {Smith, Theresa and Williamson, Charles H. D. and Hill, Karen and Sahl, Jason and Keim, Paul},
abstractNote = {Botulinum neurotoxins (BoNTs) are produced by a diverse set of seven clostridial species, though alternate naming systems have developed over the last 100 years. Starting in the 1950s, a single-species taxonomy where any bacterium producing BoNT would be designated Clostridium botulinum was introduced. As the extreme diversity of these strains was recognized, a secondary system of taxonomic “groups” evolved. It became clear that these groups also had members that did not produce BoNT, and in some cases, they were given formal species names. Genomic analysis now clearly identifies species affiliations whether an isolate is toxigenic or not. It is clear that C. botulinum group nomenclature is no longer appropriate and that there are recognized species names for each clostridium. We advocate for the use of the scientific binomials and that the single-species group nomenclature be abandoned.},
doi = {10.1128/mBio.01469-18},
journal = {mBio (Online)},
number = 5,
volume = 9,
place = {United States},
year = {Tue Sep 25 00:00:00 EDT 2018},
month = {Tue Sep 25 00:00:00 EDT 2018}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1128/mBio.01469-18
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Cited by: 30 works
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Figures / Tables:

TABLE 1 TABLE 1: Phenotypic characteristics of BoNT-producing clostridia
All figures and tables (2 total)
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Works referenced in this record:

Beiträge zur Kenntnis des Toxins und Antitoxins des Bacillus botulinus
journal, December 1910

  • Leuchs, J.
  • Zeitschrift für Hygiene und Infektionskrankheiten, Vol. 65, Issue 1
  • DOI: 10.1007/bf02284114

The Occurrence of Nontoxic Strains of CL. Parabotulinum. XXXIV
journal, August 1929

Phylogeny and taxonomy of the food-borne pathogen Clostridium botulinum and its neurotoxins
journal, January 1998

ModelFinder: fast model selection for accurate phylogenetic estimates
journal, May 2017

  • Kalyaanamoorthy, Subha; Minh, Bui Quang; Wong, Thomas K. F.
  • Nature Methods, Vol. 14, Issue 6
  • DOI: 10.1038/nmeth.4285

Preliminary Note on a Toxin-Producing Anaerobe Isolated from the Larvæ of Lucilia Cæsar
journal, January 1922

  • Bengtson, Ida A.
  • Public Health Reports (1896-1970), Vol. 37, Issue 4
  • DOI: 10.2307/4576258

Pathogenicity of Clostridium Botulinum
journal, April 1925

QIIME allows analysis of high-throughput community sequencing data
journal, April 2010

  • Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
  • Nature Methods, Vol. 7, Issue 5
  • DOI: 10.1038/nmeth.f.303

Batch procedure for thermal elution of DNA from hydroxyapatite
journal, January 1969

Notes on Bacillus Botulinus
journal, January 1919

Clostridium botulinum group III: a group with dual identity shaped by plasmids, phages and mobile elements
journal, April 2011

Two Cases of Type E Infant Botulism Caused by Neurotoxigenic Clostridium butyricum in Italy
journal, August 1986

MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004

  • Edgar, R. C.
  • Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
  • DOI: 10.1093/nar/gkh340

Genomic insights that advance the species definition for prokaryotes
journal, February 2005

  • Konstantinidis, K. T.; Tiedje, J. M.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 7
  • DOI: 10.1073/pnas.0409727102

Bacterial evolution.
journal, January 1987

Botulism
book, January 1988

Ecology of Neurotoxigenic Strains of Clostridia
book, January 1995

IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies
journal, November 2014

  • Nguyen, Lam-Tung; Schmidt, Heiko A.; von Haeseler, Arndt
  • Molecular Biology and Evolution, Vol. 32, Issue 1
  • DOI: 10.1093/molbev/msu300

European Strains of CL. Botulinum. XXXVI
journal, August 1929

Mash: fast genome and metagenome distance estimation using MinHash
journal, June 2016

Bacteriophages of Clostridium botulinum types A, B, E, and F and nontoxigenic strains resembling type E.
journal, January 1969

Genetic Diversity among Botulinum Neurotoxin-Producing Clostridial Strains
journal, November 2006

  • Hill, K. K.; Smith, T. J.; Helma, C. H.
  • Journal of Bacteriology, Vol. 189, Issue 3
  • DOI: 10.1128/JB.01180-06

Phylogenetic basis for a taxonomic dissection of the genus Clostridium
journal, July 1999

Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism.
journal, January 1985

3 16 S Ribosomal RNA Oligonucleotide Cataloguing
book, January 1985

Bacterial evolution
journal, June 1987

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    Works referencing / citing this record:

    The Copy Number of the spoVA 2mob Operon Determines Pressure Resistance of Bacillus Endospores
    journal, August 2019

    • Li, Zhen; Schottroff, Felix; Simpson, David J.
    • Applied and Environmental Microbiology, Vol. 85, Issue 19
    • DOI: 10.1128/aem.01596-19

    Public Health Risk Associated with Botulism as Foodborne Zoonoses
    journal, December 2019

    • Rasetti-Escargueil, Christine; Lemichez, Emmanuel; Popoff, Michel R.
    • Toxins, Vol. 12, Issue 1
    • DOI: 10.3390/toxins12010017
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      Figures / Tables found in this record:

      TABLE 1 (p. 3)table
      FIG 1 (p. 5)figure
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