Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Swasey, Steven M.; Nicholson, Hunter C.; Copp, Stacy M.; Bogdanov, Petko; Gorovits, Alexander; Gwinn, Elisabeth G.

doi:10.1063/1.5023258

Title: Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Journal Article · 24 September 2018 · Review of Scientific Instruments

DOI: https://doi.org/10.1063/1.5023258 · OSTI ID:1477657

Swasey, Steven M. ^[1]; Nicholson, Hunter C. ^[1];

^[2];

^[3]; Gorovits, Alexander ^[3];

^[1]

Univ. of California, Santa Barbara, CA (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
University at Albany, SUNY, Albany, NY (United States)

In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Los Alamos National Laboratory (LANL)

Sponsoring Organization:: USDOE Office of Science (SC); Laboratory Directed Research and Development (LDRD)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1477657

Alternate ID(s):: OSTI ID: 1473743

Report Number(s):: LA-UR-18-21199

Journal Information:: Review of Scientific Instruments, Journal Name: Review of Scientific Instruments Journal Issue: 9 Vol. 89; ISSN 0034-6748

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

References (33)

High-throughput and ultra-high-throughput screening: solution- and cell-based approaches Sundberg, S. Current Opinion in Biotechnology, Vol. 11, Issue 1 https://doi.org/10.1016/s0958-1669(99)00051-8	journal	February 2000
Transmission in near-infrared optical windows for deep brain imaging Shi, Lingyan; Sordillo, Laura A.; Rodríguez-Contreras, Adrián Journal of Biophotonics, Vol. 9, Issue 1-2 https://doi.org/10.1002/jbio.201500192	journal	November 2015
Design and Synthesis of Near-infrared Fluorescent Probes for Imaging of Biological Nitroxyl Tan, Yi; Liu, Ruochuan; Zhang, Huatang Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep16979	journal	November 2015
A microplate fluorimetric assay for the study of enzyme diversity in soils Marx, M. -C; Wood, M.; Jarvis, S. C. Soil Biology and Biochemistry, Vol. 33, Issue 12-13 https://doi.org/10.1016/s0038-0717(01)00079-7	journal	October 2001
Zebrafish: A Preclinical Model for Drug Screening Parng, Chuenlei; Seng, Wen Lin; Semino, Carlos ASSAY and Drug Development Technologies, Vol. 1, Issue 1 https://doi.org/10.1089/154065802761001293	journal	November 2002
Magic Numbers in DNA-Stabilized Fluorescent Silver Clusters Lead to Magic Colors Copp, Stacy M.; Schultz, Danielle; Swasey, Steven The Journal of Physical Chemistry Letters, Vol. 5, Issue 6 https://doi.org/10.1021/jz500146q	journal	March 2014
Base-Directed Formation of Fluorescent Silver Clusters Sengupta, Bidisha; Ritchie, Caroline M.; Buckman, Jenna G. The Journal of Physical Chemistry C, Vol. 112, Issue 48 https://doi.org/10.1021/jp804031v	journal	November 2008
High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery Pantoliano, M. W.; Petrella, E. C.; Kwasnoski, J. D. Journal of Biomolecular Screening, Vol. 6, Issue 6 https://doi.org/10.1177/108705710100600609	journal	December 2001
A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases Zhou, Mingjie; Diwu, Zhenjun; Panchuk-Voloshina, Nataliya Analytical Biochemistry, Vol. 253, Issue 2 https://doi.org/10.1006/abio.1997.2391	journal	November 1997
High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery Pantoliano, Michael W.; Petrella, Eugene C.; Kwasnoski, Joseph D. Journal of Biomolecular Screening, Vol. 6, Issue 6 https://doi.org/10.1089/108705701753364922	journal	December 2001
Fluorescence Color by Data-Driven Design of Genomic Silver Clusters Copp, Stacy M.; Gorovits, Alexander; Swasey, Steven M. ACS Nano, Vol. 12, Issue 8 https://doi.org/10.1021/acsnano.8b03404	journal	July 2018
Evidence for Rod-Shaped DNA-Stabilized Silver Nanocluster Emitters Schultz, Danielle; Gardner, Kira; Oemrawsingh, Sumant S. R. Advanced Materials, Vol. 25, Issue 20 https://doi.org/10.1002/adma.201204624	journal	February 2013
Biochemical quantification of DNA in human articular and septal cartilage using PicoGreen® and Hoechst 33258 McGowan, K. B.; Kurtis, M. S.; Lottman, L. M. Osteoarthritis and Cartilage, Vol. 10, Issue 7 https://doi.org/10.1053/joca.2002.0794	journal	July 2002
Quantum dots, lighting up the research and development of nanomedicine Wang, Yunqing; Chen, Lingxin Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 7, Issue 4 https://doi.org/10.1016/j.nano.2010.12.006	journal	August 2011
Naphthoquinones inhibit formation and viability of Yersinia enterocolitica biofilm Di Marco, Natalia I.; Páez, Paulina L.; Lucero-Estrada, Cecilia S. M. World Journal of Microbiology and Biotechnology, Vol. 37, Issue 2 https://doi.org/10.1007/s11274-020-02971-7	journal	January 2021
Near-Infrared Fluorescent Nanoprobes for in Vivo Optical Imaging Quek, Chai-Hoon; Leong, Kam W. Nanomaterials, Vol. 2, Issue 2 https://doi.org/10.3390/nano2020092	journal	March 2012
Optically Enhanced, Near-IR, Silver Cluster Emission Altered by Single Base Changes in the DNA Template Petty, Jeffrey T.; Fan, Chaoyang; Story, Sandra P. The Journal of Physical Chemistry B, Vol. 115, Issue 24 https://doi.org/10.1021/jp202024x	journal	June 2011
Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader11Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable. Wang, Hong; Joseph, James A. Free Radical Biology and Medicine, Vol. 27, Issue 5-6 https://doi.org/10.1016/s0891-5849(99)00107-0	journal	September 1999
Near-infrared emitting probes for biological imaging: Organic fluorophores, quantum dots, fluorescent proteins, lanthanide(III) complexes and nanomaterials Martinić, Ivana; Eliseeva, Svetlana V.; Petoud, Stéphane Journal of Luminescence, Vol. 189 https://doi.org/10.1016/j.jlumin.2016.09.058	journal	September 2017
A DFT Study of Linear Gold–Thiolate Superclusters Absorbing in the Therapeutic NIR Window Malola, Sami; Lehtovaara, Lauri; Häkkinen, Hannu The Journal of Physical Chemistry Letters, Vol. 5, Issue 8 https://doi.org/10.1021/jz500449k	journal	March 2014
Sequence-Dependent Fluorescence of DNA-Hosted Silver Nanoclusters Gwinn, E. G.; O'Neill, P.; Guerrero, A. J. Advanced Materials, Vol. 20, Issue 2, p. 279-283 https://doi.org/10.1002/adma.200702380	journal	January 2008
Carbon quantum dots and their applications Lim, Shi Ying; Shen, Wei; Gao, Zhiqiang Chemical Society Reviews, Vol. 44, Issue 1 https://doi.org/10.1039/c4cs00269e	journal	January 2015
Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat Yudasaka, Masako; Yomogida, Yohei; Zhang, Minfang Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep44760	journal	March 2017
Base Motif Recognition and Design of DNA Templates for Fluorescent Silver Clusters by Machine Learning Copp, Stacy M.; Bogdanov, Petko; Debord, Mark Advanced Materials, Vol. 28, Issue 16 https://doi.org/10.1002/adma.201600801	journal	April 2016
NIR dyes for bioimaging applications Escobedo, Jorge O.; Rusin, Oleksandr; Lim, Soojin Current Opinion in Chemical Biology, Vol. 14, Issue 1 https://doi.org/10.1016/j.cbpa.2009.10.022	journal	February 2010
Fluorescence Quantum Yields of a Series of Red and Near-Infrared Dyes Emitting at 600−1000 nm Rurack, Knut; Spieles, Monika Analytical Chemistry, Vol. 83, Issue 4 https://doi.org/10.1021/ac101329h	journal	February 2011
Shortwave-infrared (SWIR) emitters for biological imaging: a review of challenges and opportunities Thimsen, Elijah; Sadtler, Bryce; Berezin, Mikhail Y. Nanophotonics, Vol. 6, Issue 5 https://doi.org/10.1515/nanoph-2017-0039	journal	June 2017
High-Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format Huang, Dejian; Ou, Boxin; Hampsch-Woodill, Maureen Journal of Agricultural and Food Chemistry, Vol. 50, Issue 16 https://doi.org/10.1021/jf0201529	journal	July 2002
Base Motif Recognition and Design of DNA Templates for Fluorescent Silver Clusters by Machine Learning Copp, Stacy M.; Bogdanov, Petko; Debord, Mark Advanced Materials, Vol. 26, Issue 33 https://doi.org/10.1002/adma.201401402	journal	July 2014
Second window for in vivo imaging Smith, Andrew M.; Mancini, Michael C.; Nie, Shuming Nature Nanotechnology, Vol. 4, Issue 11 https://doi.org/10.1038/nnano.2009.326	journal	November 2009
The Rise of Near-Infrared Emitters: Organic Dyes, Porphyrinoids, and Transition Metal Complexes Barbieri, Andrea; Bandini, Elisa; Monti, Filippo Topics in Current Chemistry, Vol. 374, Issue 4 https://doi.org/10.1007/s41061-016-0048-9	journal	July 2016
Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green Carr, Jessica A.; Franke, Daniel; Caram, Justin R. Proceedings of the National Academy of Sciences, Vol. 115, Issue 17 https://doi.org/10.1073/pnas.1718917115	journal	April 2018
The Rise of Near-Infrared Emitters: Organic Dyes, Porphyrinoids, and Transition Metal Complexes Barbieri, Andrea; Bandini, Elisa; Monti, Filippo Topics in Current Chemistry Collections https://doi.org/10.1007/978-3-319-59304-3_9	book	July 2016

Cited By (1)

High throughput near infrared screening discovers DNA-templated silver clusters with peak fluorescence beyond 950 nm Swasey, Steven M.; Copp, Stacy M.; Nicholson, Hunter C. Nanoscale, Vol. 10, Issue 42 https://doi.org/10.1039/c8nr05781h	journal	January 2018

Similar Records

Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs

Journal Article · 2017 · Analytica Chimica Acta · OSTI ID:1378036

Wang, Ying; Li, Zhaohui; Liu, Misha; +3 more

Hydroporphyrin-Doped Near-Infrared-Emitting Polymer Dots for Cellular Fluorescence Imaging

Journal Article · 2022 · ACS Applied Materials and Interfaces · OSTI ID:1868884

Riahin, Connor; Meares, Adam; Esemoto, Nopondo N.; +10 more

A stable double-stranded DNA-ethidium homodimer complex: Application to picogram fluorescence detection of DNA in agarose gels

Journal Article · 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:5324040

Glazer, A N; Mathies, R A; Peck, K

Related Subjects

47 OTHER INSTRUMENTATION
59 BASIC BIOLOGICAL SCIENCES
Material Science

Title: Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Citation Formats

References (33)

Cited By (1)

Similar Records

Related Subjects