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Title: Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Journal Article · · Review of Scientific Instruments
DOI: https://doi.org/10.1063/1.5023258 · OSTI ID:1477657

In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.

Research Organization:
Los Alamos National Laboratory (LANL)
Sponsoring Organization:
USDOE Office of Science (SC); Laboratory Directed Research and Development (LDRD)
Grant/Contract Number:
AC52-06NA25396
OSTI ID:
1477657
Alternate ID(s):
OSTI ID: 1473743
Report Number(s):
LA-UR-18-21199
Journal Information:
Review of Scientific Instruments, Journal Name: Review of Scientific Instruments Journal Issue: 9 Vol. 89; ISSN 0034-6748
Publisher:
American Institute of Physics (AIP)Copyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

High throughput near infrared screening discovers DNA-templated silver clusters with peak fluorescence beyond 950 nm journal January 2018

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