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Title: Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Abstract

In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.

Authors:
 [1];  [1]; ORCiD logo [2]; ORCiD logo [3];  [3]; ORCiD logo [1]
+ Show Author Affiliations
  1. Univ. of California, Santa Barbara, CA (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  3. University at Albany, SUNY, Albany, NY (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1477657
Alternate Identifier(s):
OSTI ID: 1473743
Report Number(s):
LA-UR-18-21199
Journal ID: ISSN 0034-6748
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Accepted Manuscript
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 89; Journal Issue: 9; Journal ID: ISSN 0034-6748
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; Material Science

Citation Formats

Swasey, Steven M., Nicholson, Hunter C., Copp, Stacy M., Bogdanov, Petko, Gorovits, Alexander, and Gwinn, Elisabeth G. Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes. United States: N. p., 2018. Web. doi:10.1063/1.5023258.
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Swasey, Steven M., Nicholson, Hunter C., Copp, Stacy M., Bogdanov, Petko, Gorovits, Alexander, & Gwinn, Elisabeth G. Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes. United States. https://doi.org/10.1063/1.5023258
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Swasey, Steven M., Nicholson, Hunter C., Copp, Stacy M., Bogdanov, Petko, Gorovits, Alexander, and Gwinn, Elisabeth G. Tue . "Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes". United States. https://doi.org/10.1063/1.5023258. https://www.osti.gov/servlets/purl/1477657.
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@article{osti_1477657,
title = {Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes},
author = {Swasey, Steven M. and Nicholson, Hunter C. and Copp, Stacy M. and Bogdanov, Petko and Gorovits, Alexander and Gwinn, Elisabeth G.},
abstractNote = {In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.},
doi = {10.1063/1.5023258},
journal = {Review of Scientific Instruments},
number = 9,
volume = 89,
place = {United States},
year = {2018},
month = {9}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1063/1.5023258
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Cited by: 1 work
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Figures / Tables:

FIG. 1. FIG. 1. : (a) Schematic of the instrument adaptation, here applied to a Tecan Infinite 200 Pro microplate reader. The blue box indicates the chassis of the microplate reader. Orange circles indicate elements added for the NIR adaptation. The excitation and emission filters are placed in the filter holder suppliedmore » with the instrument. The InGaAs PIN photodetector is added to the fluorescence detection path using a simple, home-machined cylindrical adapter that contains a focusing lens. The signal cable from the detector passes through the microplate reader chassis to an envelope filter consisting of a 1N4001 diode with an RC filter (R = 1 MΩ, C = 220 nF) that is mounted on a circuit board. A USB-based digitizer records the signal output of the envelope filter. (b) Typical spectrum of a xenon flashlamp with a UV glass or synthetic silica housing, adapted from Hamamatsu’s online spectra (relative intensities vary depending on flashlamp details).« less
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Works referenced in this record:

High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery
journal, December 2001

  • Pantoliano, M. W.; Petrella, E. C.; Kwasnoski, J. D.
  • Journal of Biomolecular Screening, Vol. 6, Issue 6
  • DOI: 10.1177/108705710100600609

Base Motif Recognition and Design of DNA Templates for Fluorescent Silver Clusters by Machine Learning
journal, July 2014

  • Copp, Stacy M.; Bogdanov, Petko; Debord, Mark
  • Advanced Materials, Vol. 26, Issue 33
  • DOI: 10.1002/adma.201401402

Sequence-Dependent Fluorescence of DNA-Hosted Silver Nanoclusters
journal, January 2008

  • Gwinn, E. G.; O'Neill, P.; Guerrero, A. J.
  • Advanced Materials, Vol. 20, Issue 2, p. 279-283
  • DOI: 10.1002/adma.200702380

Evidence for Rod-Shaped DNA-Stabilized Silver Nanocluster Emitters
journal, February 2013

  • Schultz, Danielle; Gardner, Kira; Oemrawsingh, Sumant S. R.
  • Advanced Materials, Vol. 25, Issue 20
  • DOI: 10.1002/adma.201204624

Base Motif Recognition and Design of DNA Templates for Fluorescent Silver Clusters by Machine Learning
journal, April 2016

  • Copp, Stacy M.; Bogdanov, Petko; Debord, Mark
  • Advanced Materials, Vol. 28, Issue 16
  • DOI: 10.1002/adma.201600801

Transmission in near-infrared optical windows for deep brain imaging
journal, November 2015

  • Shi, Lingyan; Sordillo, Laura A.; Rodríguez-Contreras, Adrián
  • Journal of Biophotonics, Vol. 9, Issue 1-2
  • DOI: 10.1002/jbio.201500192

The Rise of Near-Infrared Emitters: Organic Dyes, Porphyrinoids, and Transition Metal Complexes
journal, July 2016

  • Barbieri, Andrea; Bandini, Elisa; Monti, Filippo
  • Topics in Current Chemistry, Vol. 374, Issue 4
  • DOI: 10.1007/s41061-016-0048-9

NIR dyes for bioimaging applications
journal, February 2010

  • Escobedo, Jorge O.; Rusin, Oleksandr; Lim, Soojin
  • Current Opinion in Chemical Biology, Vol. 14, Issue 1
  • DOI: 10.1016/j.cbpa.2009.10.022

Near-infrared emitting probes for biological imaging: Organic fluorophores, quantum dots, fluorescent proteins, lanthanide(III) complexes and nanomaterials
journal, September 2017

Quantum dots, lighting up the research and development of nanomedicine
journal, August 2011

  • Wang, Yunqing; Chen, Lingxin
  • Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 7, Issue 4
  • DOI: 10.1016/j.nano.2010.12.006

High-throughput and ultra-high-throughput screening: solution- and cell-based approaches
journal, February 2000

Fluorescence Quantum Yields of a Series of Red and Near-Infrared Dyes Emitting at 600−1000 nm
journal, February 2011

  • Rurack, Knut; Spieles, Monika
  • Analytical Chemistry, Vol. 83, Issue 4
  • DOI: 10.1021/ac101329h

Fluorescence Color by Data-Driven Design of Genomic Silver Clusters
journal, July 2018

High-Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format
journal, July 2002

  • Huang, Dejian; Ou, Boxin; Hampsch-Woodill, Maureen
  • Journal of Agricultural and Food Chemistry, Vol. 50, Issue 16
  • DOI: 10.1021/jf0201529

Optically Enhanced, Near-IR, Silver Cluster Emission Altered by Single Base Changes in the DNA Template
journal, June 2011

  • Petty, Jeffrey T.; Fan, Chaoyang; Story, Sandra P.
  • The Journal of Physical Chemistry B, Vol. 115, Issue 24
  • DOI: 10.1021/jp202024x

Base-Directed Formation of Fluorescent Silver Clusters
journal, November 2008

  • Sengupta, Bidisha; Ritchie, Caroline M.; Buckman, Jenna G.
  • The Journal of Physical Chemistry C, Vol. 112, Issue 48
  • DOI: 10.1021/jp804031v

Magic Numbers in DNA-Stabilized Fluorescent Silver Clusters Lead to Magic Colors
journal, March 2014

  • Copp, Stacy M.; Schultz, Danielle; Swasey, Steven
  • The Journal of Physical Chemistry Letters, Vol. 5, Issue 6
  • DOI: 10.1021/jz500146q

A DFT Study of Linear Gold–Thiolate Superclusters Absorbing in the Therapeutic NIR Window
journal, March 2014

  • Malola, Sami; Lehtovaara, Lauri; Häkkinen, Hannu
  • The Journal of Physical Chemistry Letters, Vol. 5, Issue 8
  • DOI: 10.1021/jz500449k

Second window for in vivo imaging
journal, November 2009

  • Smith, Andrew M.; Mancini, Michael C.; Nie, Shuming
  • Nature Nanotechnology, Vol. 4, Issue 11
  • DOI: 10.1038/nnano.2009.326

Design and Synthesis of Near-infrared Fluorescent Probes for Imaging of Biological Nitroxyl
journal, November 2015

  • Tan, Yi; Liu, Ruochuan; Zhang, Huatang
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep16979

Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat
journal, March 2017

  • Yudasaka, Masako; Yomogida, Yohei; Zhang, Minfang
  • Scientific Reports, Vol. 7, Issue 1
  • DOI: 10.1038/srep44760

Carbon quantum dots and their applications
journal, January 2015

  • Lim, Shi Ying; Shen, Wei; Gao, Zhiqiang
  • Chemical Society Reviews, Vol. 44, Issue 1
  • DOI: 10.1039/c4cs00269e

Biochemical quantification of DNA in human articular and septal cartilage using PicoGreen® and Hoechst 33258
journal, July 2002

  • McGowan, K. B.; Kurtis, M. S.; Lottman, L. M.
  • Osteoarthritis and Cartilage, Vol. 10, Issue 7
  • DOI: 10.1053/joca.2002.0794

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green
journal, April 2018

  • Carr, Jessica A.; Franke, Daniel; Caram, Justin R.
  • Proceedings of the National Academy of Sciences, Vol. 115, Issue 17
  • DOI: 10.1073/pnas.1718917115

High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery
journal, December 2001

  • Pantoliano, Michael W.; Petrella, Eugene C.; Kwasnoski, Joseph D.
  • Journal of Biomolecular Screening, Vol. 6, Issue 6
  • DOI: 10.1089/108705701753364922

Zebrafish: A Preclinical Model for Drug Screening
journal, November 2002

  • Parng, Chuenlei; Seng, Wen Lin; Semino, Carlos
  • ASSAY and Drug Development Technologies, Vol. 1, Issue 1
  • DOI: 10.1089/154065802761001293

Shortwave-infrared (SWIR) emitters for biological imaging: a review of challenges and opportunities
journal, June 2017

Near-Infrared Fluorescent Nanoprobes for in Vivo Optical Imaging
journal, March 2012

Naphthoquinones inhibit formation and viability of Yersinia enterocolitica biofilm
journal, January 2021

  • Di Marco, Natalia I.; Páez, Paulina L.; Lucero-Estrada, Cecilia S. M.
  • World Journal of Microbiology and Biotechnology, Vol. 37, Issue 2
  • DOI: 10.1007/s11274-020-02971-7
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    Works referencing / citing this record:

    High throughput near infrared screening discovers DNA-templated silver clusters with peak fluorescence beyond 950 nm
    journal, January 2018

    • Swasey, Steven M.; Copp, Stacy M.; Nicholson, Hunter C.
    • Nanoscale, Vol. 10, Issue 42
    • DOI: 10.1039/c8nr05781h
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