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Title: Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.
Authors:
 [1] ;  [1] ; ORCiD logo [2] ; ORCiD logo [3] ;  [3] ; ORCiD logo [1]
  1. Univ. of California, Santa Barbara, CA (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  3. University at Albany, SUNY, Albany, NY (United States)
Publication Date:
Report Number(s):
LA-UR-18-21199
Journal ID: ISSN 0034-6748
Grant/Contract Number:
AC52-06NA25396
Type:
Accepted Manuscript
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 89; Journal Issue: 9; Journal ID: ISSN 0034-6748
Publisher:
American Institute of Physics (AIP)
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1477657
Alternate Identifier(s):
OSTI ID: 1473743

Swasey, Steven M., Nicholson, Hunter C., Copp, Stacy M., Bogdanov, Petko, Gorovits, Alexander, and Gwinn, Elisabeth G.. Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes. United States: N. p., Web. doi:10.1063/1.5023258.
Swasey, Steven M., Nicholson, Hunter C., Copp, Stacy M., Bogdanov, Petko, Gorovits, Alexander, & Gwinn, Elisabeth G.. Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes. United States. doi:10.1063/1.5023258.
Swasey, Steven M., Nicholson, Hunter C., Copp, Stacy M., Bogdanov, Petko, Gorovits, Alexander, and Gwinn, Elisabeth G.. 2018. "Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes". United States. doi:10.1063/1.5023258. https://www.osti.gov/servlets/purl/1477657.
@article{osti_1477657,
title = {Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes},
author = {Swasey, Steven M. and Nicholson, Hunter C. and Copp, Stacy M. and Bogdanov, Petko and Gorovits, Alexander and Gwinn, Elisabeth G.},
abstractNote = {In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.},
doi = {10.1063/1.5023258},
journal = {Review of Scientific Instruments},
number = 9,
volume = 89,
place = {United States},
year = {2018},
month = {9}
}

Works referenced in this record:

Sequence-Dependent Fluorescence of DNA-Hosted Silver Nanoclusters
journal, January 2008
  • Gwinn, E.?G.; O'Neill, P.; Guerrero, A.?J.
  • Advanced Materials, Vol. 20, Issue 2, p. 279-283
  • DOI: 10.1002/adma.200702380