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Title: Carbon use efficiency diagnostics in Nannochloropsis salina

Abstract

We report that carbon dioxide (CO 2) can be a significant resource input affecting the cost of algae biomass production on an industrial scale. Improvements in biofuel productivity therefore require characterization of CO 2 use efficiency (CUE). RuBisCO saturation with CO 2 is an important factor influencing biomass productivity. During CO2 fixation by RuBisCO, fractionation of carbon isotopes occurs, with preferential fixation of 12CO 2, resulting in assimilation of the lighter isotope in algae. This photosynthetic discrimination (Δ DIC-algae), approximated by the difference between the δ 13C of external medium and that of algae, is a function of the proportion of CO 2 fixed relative to supply. Δ DIC-algae has been applied to the study of photosynthesis in algae over the past few decades and we have adopted the tool to characterize improvements in conditions optimized for biofuel productivity, such as controlled photobioreactors. We report the use of Δ DIC-algae as a tool for characterizing CO 2 dynamics and RuBisCO saturation in Nannochloropsis salina CCMP 1776, a benchmark strain in algal biofuels research. We use Δ DIC-algae to describe the conditions under which RuBisCO saturation can be achieved to maximize biomass productivity. Finally, our results suggest that determination of Δmore » DIC-algae can provide important feedback to support engineering and cultural improvements that can impact carbon use efficiency and biomass productivity.« less

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1477645
Alternate Identifier(s):
OSTI ID: 1547154
Report Number(s):
LA-UR-16-23459
Journal ID: ISSN 2211-9264
Grant/Contract Number:  
AC52-06NA25396; 20150226ER
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 31; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; Biofuels; Carbon concentrating mechanisms; isotope fractionation; Nannochloropsis salina; carbon use efficiency; RuBisCO

Citation Formats

Zidenga, Tawanda, Teshima, Munehiro, Perkins, George B., Rahn, Thomas A., Twary, Scott Nicholas, and Heikoop, Jeffrey Martin. Carbon use efficiency diagnostics in Nannochloropsis salina. United States: N. p., 2018. Web. doi:10.1016/j.algal.2018.01.011.
Zidenga, Tawanda, Teshima, Munehiro, Perkins, George B., Rahn, Thomas A., Twary, Scott Nicholas, & Heikoop, Jeffrey Martin. Carbon use efficiency diagnostics in Nannochloropsis salina. United States. doi:10.1016/j.algal.2018.01.011.
Zidenga, Tawanda, Teshima, Munehiro, Perkins, George B., Rahn, Thomas A., Twary, Scott Nicholas, and Heikoop, Jeffrey Martin. Sat . "Carbon use efficiency diagnostics in Nannochloropsis salina". United States. doi:10.1016/j.algal.2018.01.011. https://www.osti.gov/servlets/purl/1477645.
@article{osti_1477645,
title = {Carbon use efficiency diagnostics in Nannochloropsis salina},
author = {Zidenga, Tawanda and Teshima, Munehiro and Perkins, George B. and Rahn, Thomas A. and Twary, Scott Nicholas and Heikoop, Jeffrey Martin},
abstractNote = {We report that carbon dioxide (CO2) can be a significant resource input affecting the cost of algae biomass production on an industrial scale. Improvements in biofuel productivity therefore require characterization of CO2 use efficiency (CUE). RuBisCO saturation with CO2 is an important factor influencing biomass productivity. During CO2 fixation by RuBisCO, fractionation of carbon isotopes occurs, with preferential fixation of 12CO2, resulting in assimilation of the lighter isotope in algae. This photosynthetic discrimination (ΔDIC-algae), approximated by the difference between the δ13C of external medium and that of algae, is a function of the proportion of CO2 fixed relative to supply. ΔDIC-algae has been applied to the study of photosynthesis in algae over the past few decades and we have adopted the tool to characterize improvements in conditions optimized for biofuel productivity, such as controlled photobioreactors. We report the use of ΔDIC-algae as a tool for characterizing CO2 dynamics and RuBisCO saturation in Nannochloropsis salina CCMP 1776, a benchmark strain in algal biofuels research. We use ΔDIC-algae to describe the conditions under which RuBisCO saturation can be achieved to maximize biomass productivity. Finally, our results suggest that determination of ΔDIC-algae can provide important feedback to support engineering and cultural improvements that can impact carbon use efficiency and biomass productivity.},
doi = {10.1016/j.algal.2018.01.011},
journal = {Algal Research},
number = C,
volume = 31,
place = {United States},
year = {2018},
month = {2}
}

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